LOCUS       Exported                7752 bp ds-DNA     circular SYN 28-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   mqbssrl3
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 25, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             686..1501
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(1594..2049)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     terminator      2086..2133
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     gene            complement(2239..2955)
                     /label=sfGFP
     RBS             3365..3373
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     gene            3381..3857
                     /label=ferC
     terminator      3858..3901
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             4056..4064
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     gene            4072..6201
                     /label=ferA
     terminator      6202..6245
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             7107..7298
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    7400..7542
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(join(7728..7752,1..564))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
       61 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      121 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      181 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      241 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      301 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      361 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      421 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      481 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      541 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      601 cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta
      661 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag
      721 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
      781 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
      841 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
      901 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
      961 tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga
     1021 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     1081 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     1141 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     1201 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     1261 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     1321 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     1381 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     1441 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     1501 agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     1561 ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa
     1621 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     1681 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     1741 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     1801 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     1861 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     1921 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     1981 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     2041 agggcgcgtc ccattcgcca atccggatat agttcctcct ttcagcaaaa aacccctcaa
     2101 gacccgttta gaggccccaa ggggttatgc tagttattgc tcagcggtgg cagcagccaa
     2161 ctcagcttcc tttcgggctt tgttacatgc ggtaccgaga aatcatcctt agcgaaagct
     2221 aaggattttt tttatctgtt atttatacag ttcatccata ccatgggtaa tacctgctgc
     2281 ggtaacaaat tccagcagaa ccatgtgatc acgtttttcg ttcggatctt tgctcagaac
     2341 gctctgggtg ctcagataat gattatccgg cagcagcacc ggaccatcac caatcggggt
     2401 attctgctga taatgatctg ccagctgaac gctaccatct tccacattat gccgaatttt
     2461 gaagttggct ttgatgccat ttttctgttt gtctgcggtg atatacacgt tgtggctatt
     2521 gaaattatat tccagtttgt ggcccaggat attgccatct tctttaaaat cgatgccttt
     2581 cagttcaatg cgattcacca gggtatcacc ttcaaattta acttcggcac gggttttata
     2641 ggtgccatca tctttgaagc tgatggtacg ttcttgaaca taaccttccg gcattgcgct
     2701 tttgaaaaaa tcgtggcgtt tcatgtgatc cggataacgg ctaaaacact gaacaccata
     2761 ggtcagggtg gtaaccaggg tcggccacgg aaccggcagt ttgccggtgg tacagataaa
     2821 tttcagggtc agtttaccat tggttgcatc accttcgcct tcaccacgaa ctgaaaattt
     2881 gtggccatta acatcaccat ccagttcaac cagaatcgga acaacaccgg taaacagttc
     2941 ttcacctttg ctcatatgta tatctccttc ttaaagttaa atcggtcagt gcgtcatgct
     3001 ataagccata gcatacctag gactgagcta gctgtcaaat gctataagcc atagcattgc
     3061 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg gcgtcaggca
     3121 gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct
     3181 caaggcgcac tcccgttctg gataatgttt tttgcccaca gctaacacca cgtcgtccct
     3241 atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc
     3301 gtacaatata ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt
     3361 gaaataagga ggtaatacaa atgggtgaac gtatgcgtca ggatgatcag gatgcaggca
     3421 ccggtagcac cgatgcaccg gctgcactgc tgccgagtcc gctggataat atgctgggtt
     3481 atcgtattcg tcgtgcaagc atggcaatga tgcatgatct ggctcatgca ctgcgtccgt
     3541 ttgatattag cgttggtgaa gcaagcctgc tgattatggt tggtgcaaat ccgggttgtc
     3601 gtcagagcga tctgggtcgt gcactggaaa ttaaacgtgc aaatatgaca ccgctggttg
     3661 gtcgtctgaa agcacgtggt ctgattacag atgcaccgat tgatggtcgt agcctgagcc
     3721 tgaccctgac cgaagaaggt gaagcactgc agaaagttct gctggcacat gcccagacca
     3781 gcaatgatcg ttttcgtaaa cgtctggcac tggatgaaaa agatctgatt gcagcaatgg
     3841 cagcactggc acgttgaata aaacgaaagg ctcagtcgga agactgggcc tttcgtttta
     3901 ttctagacac agctaacacc acgtcgtccc tatctgctgc cctaggtcta tgagtggttg
     3961 ctggataact ttacgggcat gcataaggct cgtacaatat attcagggag accacaacgg
     4021 tttccctcta caaataattt tgtttaactt tgaaataagg aggtaataca aatggccgtt
     4081 gaagccggtg ttcgtcctca ggcaggcgca cgtgatatta atcgtctgct gcgtccgcgt
     4141 agcattgcaa ttgttggtgc aagcgaaaca ccgggtagcc tgggtgcaag cgttctggca
     4201 aatctggttc gtaatgaatt tccgggtgat attcatctgg ttaatccgaa acgtgaaacc
     4261 attagcggtc gtccggcagt tccgagcgtt gatgcactgc cggaaggtgt tgattgtgca
     4321 attctggcaa ttccgcgtgt tgcagttctg gataccatgc gtcagctggc agcacgtaaa
     4381 gccggtgcag caattatctt tgcagcaggt tttgccgaag gtggtgaaca gggtatggca
     4441 gatcagcaag aaattggtcg tattgcacat gaagcaggta ttgttgttga aggtccgaat
     4501 tgtctgggta gcgttaatta tctggatcgt attccgctga cctttatcga taccgatatt
     4561 aaagcaccga gtccgggtgg tgttggtatt gttagccaga gcggtgcaat ggcagccgtt
     4621 ctggcagtta tgctggaaag ccgtaccctg gatctgacct atagcgttag caccggtaat
     4681 gaagccggta gcggtgttga agattatgtg gaatttatga tcgccgatga aaaaacccgt
     4741 attattgcca tgattgtgga acagtttcgt gatccggctc gttttctggc actgatggat
     4801 aaagcaaatg cagcaggtaa actggttgtt ctgctgcatc cgggtaaaag cagcgcagca
     4861 cgtgaaagcg cagcaaccca tacaggcgca atggcaggcg attataaact gatgcgtgca
     4921 aaagttgaac gtgccggtgt tgttgttgca gaaaccctgg aagaactggg tgatattacc
     4981 gaaattgcag cacgttgtcc ggcactgccg agcggtggca cagcagttct gggtgaaagc
     5041 ggtgcactga aagcactgac actggatctg gcagaagaac tggaactggc actgccgacc
     5101 ctggatgatg gtaatgcacc ggcactgcgt gcagccctgc cggaatttgt tccggttagc
     5161 aatccgctgg atctgacagc acagggtctg gttgatccgg atatgtatta tcgtaccctg
     5221 gcagcactgt ttgaagatga tcgtgttggc accatttttg ccggtattat tcagaccaat
     5281 ccggcaacca ttggtattaa actgccaccg tttctgaaag cagttcgtga actgaaagca
     5341 accaaaccgg ttatttttgg tggtgttgat gaaggtgcag atgttccggc agattggatt
     5401 gaacagctgc gtgcagaagg tattccgtat tttccgacca ccgaacgtgc actgcgtgcc
     5461 attcgtcgtc tgagcgcagc gggtgcacgt gatgcaagcc gtaccgatgc agcaccggca
     5521 agcgttccgg cactggcaag cgaaaaaggt gttgttccgg aatataaagc aaaagcactg
     5581 ctggcaccgc tgggtattag ctttccgaaa ggtcagtttg cagcaaccgt tgaagatgca
     5641 attgcagcag ccgaagcaat tggtggtccg gttgttatga aagcacaggc agcagcactg
     5701 agccataaaa gtgatgccgg tggtgttgca ctgaatctgg ttggtgcgga agcaattcgt
     5761 gcagcatggg ataaaatgtt tgcagatgtg aaacgttatg acgccagcat tattctggat
     5821 ggtgttctga tcgaagcaat gggtgcccgt ggtctggaac tgattgttgg tgccaaaaat
     5881 gatccgcagt ggggtccggt tattctggca ggttttggtg gcgttaccgc agaaattctg
     5941 caggatgttc gtctgctgtc accggatatg accaaagaag caattgttgc agaactgggt
     6001 aaactgaaac aggcagcact gctgcatggt tatcgtggta gtccggcact ggatgttggt
     6061 gcagttgccg aactgattgg tcaggtgggt cgcctgctgc gtggcgaacc gcgtattcaa
     6121 gaactggatc tgaatccggt tgttgtttat ccggaaggtc agggtgcaat tgccctggat
     6181 gcactgatgc tggttgattg aataaaacga aaggctcagt cggaagactg ggcctttcgt
     6241 tttatggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6301 ctgacgcaat taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct
     6361 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     6421 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
     6481 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
     6541 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
     6601 tcggcgagaa gcaggccatt atcgccggca tggcggcccc acgggtgcgc atgatcgtgc
     6661 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
     6721 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
     6781 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc
     6841 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac
     6901 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc
     6961 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat
     7021 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca
     7081 gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg
     7141 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg
     7201 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct
     7261 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     7321 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     7381 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     7441 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt
     7501 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     7561 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7621 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7681 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7741 cgcccccctg ac
//
