LOCUS       Exported                4763 bp ds-DNA     circular SYN 03-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   mqbssrl3
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 25, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            complement(34..498)
                     /label=pcaV
     RBS             506..514
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             924..1640
                     /codon_start=1
                     /label=sfGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1683..1777
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1850..1941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1942..2802
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2806..2845
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2992..3100
                     /note="incP origin of transfer"
     CDS             complement(3111..3773)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      3774..4545
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      4660..4746
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggataacaat ttcacacagg tttttttcct ccttcaaccc ggtgcaactg ccggttcagc
       61 cggattacgc aggccttctg ctgcatctgc aacacgacga atcagatcaa aaaaaactgc
      121 ctgttcatct gctgccagcg gtgccagaaa aacctgattc atacgtgcaa tacgaacacc
      181 cagacgacga tgaacacgca gaccttcatc ggtcagacgc agcagtgaac gacgaccatc
      241 ctgcggatca cgaactttat ccagcagacc acgacgaccc agacggctaa caacttctgc
      301 aatggtgcta cgatccaggc caacacgttc accaacggta cgctgatcca gacccggttc
      361 tgcaaccagt gcattcagaa ctgcatactg cggactggtg gtttcttcgc taaccattgt
      421 attccacagc agataatgtg cctgttgcag acgacgtgcc agatgacccg gatgggttgc
      481 cagatcaact gctgccattt gtattacctc cttatttcaa agttaaacaa aattatttgt
      541 agagggaaac cgttgtggtc tccctgaata tatcgtacga gccttatgca tgcccgtaaa
      601 gttatccagc aaccactcat agacctaggg cagcagatag ggacgacgtg gtgttagctg
      661 tgggcaaaaa acattatcca gaacgggagt gcgccttgag cgacacgaat tatgcagtga
      721 tttacgacct gcacagccat accacagctt ccgatggctg cctgacgcca gaagcattgg
      781 tgcaccgtgc agtcgatgat aagctgtcaa acgcatgcaa aatttatcaa aaagagtgtt
      841 gactatactc agtgccctga ctatgatact tagattcata ctcagtgccc tgactatttt
      901 aactttaaga aggagatata catatgagca aaggtgaaga actgtttacc ggtgttgttc
      961 cgattctggt tgaactggat ggtgatgtta atggccacaa attttcagtt cgtggtgaag
     1021 gcgaaggtga tgcaaccaat ggtaaactga ccctgaaatt tatctgtacc accggcaaac
     1081 tgccggttcc gtggccgacc ctggttacca ccctgaccta tggtgttcag tgttttagcc
     1141 gttatccgga tcacatgaaa cgccacgatt ttttcaaaag cgcaatgccg gaaggttatg
     1201 ttcaagaacg taccatcagc ttcaaagatg atggcaccta taaaacccgt gccgaagtta
     1261 aatttgaagg tgataccctg gtgaatcgca ttgaactgaa aggcatcgat tttaaagaag
     1321 atggcaatat cctgggccac aaactggaat ataatttcaa tagccacaac gtgtatatca
     1381 ccgcagacaa acagaaaaat ggcatcaaag ccaacttcaa aattcggcat aatgtggaag
     1441 atggtagcgt tcagctggca gatcattatc agcagaatac cccgattggt gatggtccgg
     1501 tgctgctgcc ggataatcat tatctgagca cccagagcgt tctgagcaaa gatccgaacg
     1561 aaaaacgtga tcacatggtt ctgctggaat ttgttaccgc agcaggtatt acccatggta
     1621 tggatgaact gtataaataa gcggccgcgt cgtgactggg aaaaccctgg cgactagtct
     1681 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
     1741 gctcggttgc cgccgggcgt tttttattgg tgagaatcca ggggtcccca ataattacga
     1801 tttaaattag tagcccgcct aatgagcggg ctttttttta attcccctat ttgtttattt
     1861 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1921 taatattgaa aaaggaagag tatgagcatt cagcattttc gtgtggcgct gattccgttt
     1981 tttgcggcgt tttgcctgcc ggtgtttgcg catccggaaa ccctggtgaa agtgaaagat
     2041 gcggaagatc aactgggtgc gcgcgtgggc tatattgaac tggatctgaa cagcggcaaa
     2101 attctggaat cttttcgtcc ggaagaacgt tttccgatga tgagcacctt taaagtgctg
     2161 ctgtgcggtg cggttctgag ccgtgtggat gcgggccagg aacaactggg ccgtcgtatt
     2221 cattatagcc agaacgatct ggtggaatat agcccggtga ccgaaaaaca tctgaccgat
     2281 ggcatgaccg tgcgtgaact gtgcagcgcg gcgattacca tgagcgataa caccgcggcg
     2341 aacctgctgc tgacgaccat tggcggtccg aaagaactga ccgcgtttct gcataacatg
     2401 ggcgatcatg tgacccgtct ggatcgttgg gaaccggaac tgaacgaagc gattccgaac
     2461 gatgaacgtg ataccaccat gccggcagca atggcgacca ccctgcgtaa actgctgacg
     2521 ggtgagctgc tgaccctggc aagccgccag caactgattg attggatgga agcggataaa
     2581 gtggcgggtc cgctgctgcg tagcgcgctg ccggctggct ggtttattgc ggataaaagc
     2641 ggtgcgggcg aacgtggcag ccgtggcatt attgcggcgc tgggcccgga tggtaaaccg
     2701 agccgtattg tggtgattta taccaccggc agccaggcga cgatggatga acgtaaccgt
     2761 cagattgcgg aaattggcgc gagcctgatt aaacattggt aaaccgatac aattaaaggc
     2821 tccttttgga gccttttttt ttggacgacc cttgtccttt tccgctgcat aaccctgctt
     2881 cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt
     2941 ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat
     3001 aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct
     3061 ggcggtgctc aacgggaatc ctgctctgcg aggctggccg taggccggcc ctaccggcgc
     3121 ggcagcgtta cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa acacggctca
     3181 tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc ggtcaaggct
     3241 ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc gccggggccg
     3301 gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc gccatgcccc
     3361 aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa caccgacagg
     3421 cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta ggccgacacg
     3481 gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa gtccttgact
     3541 gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt ctggctgacc
     3601 accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat tgccgccgtg
     3661 ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt ttgcacccag
     3721 tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc catgcttatc
     3781 tccatgcggt aggggtgccg cacggttgcg gcaccatgcg caatcagctg caacttttcg
     3841 gcagcgcgac aacaattatg cgttgcgtaa aagtggcagt caattacaga ttttctttaa
     3901 cctacgcaat gagctattgc ggggggtgcc gcaatgagct gttgcgtacc cccctttttt
     3961 aagttgttga tttttaagtc tttcgcattt cgccctatat ctagttcttt ggtgcccaaa
     4021 gaagggcacc cctgcggggt tcccccacgc cttcggcgcg gctccccctc cggcaaaaag
     4081 tggcccctcc ggggcttgtt gatcgactgc gcggccttcg gccttgccca aggtggcgct
     4141 gcccccttgg aacccccgca ctcgccgccg tgaggctcgg ggggcaggcg ggcgggcttc
     4201 gcccttcgac tgcccccact cgcataggct tgggtcgttc caggcgcgtc aaggccaagc
     4261 cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac ttggtgtcca accggcaagc
     4321 gaagcgcgca ggccgcaggc cggaggcttt tccccagaga aaattaaaaa aattgatggg
     4381 gcaaggccgc aggccgcgca gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg
     4441 gtgggcaatc cctgtggtca agctcgtggg caggcgcagc ctgtccatca gcttgtccag
     4501 cagggttgtc cacgggccga gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc
     4561 acatatccac gggctggcaa gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa
     4621 gcccgtaggg ggggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc
     4681 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     4741 tatttgatgc ctttaattaa agc
//
