LOCUS       Exported                6523 bp ds-DNA     circular SYN 24-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    VEC1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 25, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    64..213
                     /label=spacer
     misc_feature    276..309
                     /label=Prom -0.7
     misc_feature    357..376
                     /label=RBS
     misc_feature    377..1138
                     /label=TphR (TB4)
     misc_feature    1139..1178
                     /label=rrnC
     misc_feature    1195..1222
                     /label=Prom
     misc_feature    1225..1250
                     /label=RBS -1
     CDS             1251..2591
                     /codon_start=1
                     /label=TphK
                     /translation="MLPESKPLQLPQFVDERRVSPFQYGVIILCGLVMFLDGFDTQAIS
                     YMAPYIAQEWSLSKQMLGPIFSSSLAGLMVGYLVLTPLSERFGHKKAIIVSTIVFSLCT
                     LASVWATNVTELMVLRFITGIGLGTAAPSAIAMTGEYSPKRLRATFVLAIYCGFSLGFI
                     AAGLAAGWFIPHYGWRSMFWVGALAPLILVPVLFRFLPESMVFMIKNKVPPQQILNVFR
                     KIDASVSQQSKPVFVVEALEQGQHGALASLFTRDRIMGTVLLWLVFAINLGEFYALQSW
                     LPSIMTGLNYPMSTVVMATTLTTVGGIAAAFITGPSMDRLGAYKTVGILYLVGFVFVAL
                     TGMAFNSPLWVLLSANFLAGCCISGGQKSLIALAAVFYPAPMRSTGVGWALGIGRIGGI
                     AGPIVVGAALGIGWSPSAVFYGMAIPMLIAGLIVLFLGRRYGNSDKV"
     terminator      2643..2729
                     /label=rrnB T1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     rep_origin      2844..3615
                     /label=pBBR1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.3"
                     /note="/other=rep_origin"
     CDS             3616..4278
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     oriT            complement(4289..4397)
                     /direction=LEFT
                     /label=oriT
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.1"
                     /note="/other=oriT"
     terminator      complement(4544..4583)
                     /label=fd terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             complement(4590..5447)
                     /codon_start=1
                     /label=bla
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5448..5539)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     terminator      complement(5612..5706)
                     /label=lambda t0 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             complement(5717..5741)
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=14.4"
                     /note="/other=CDS"
                     /translation="RRDWENPG"
     CDS             complement(5752..6465)
                     /codon_start=1
                     /label=GFP
                     /label=Superfolder GFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    6466..6491
                     /label=RBS +1
     misc_feature    join(6492..6523,1..57)
                     /label=Prom -0.3
ORIGIN
        1 cgccacaagc tagatgtgcg ccattcgaaa acttctgctg caacgcaagg cctcgccctc
       61 gaggtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc
      121 atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa
      181 ggcgcactcc cgttctggat aatgtttttt gcccacagct aacaccacgt cgtccctatc
      241 tgctgcccta ggtctatgag tggttgctgg ataactttac ggcatgcata aggctcgtag
      301 tatatattca gggagaccac aacggtttcc ctctacaaat aattttgttt aactttgaaa
      361 taaggaggta atacaaatgt cggataaaga tttcgtcacc gccctgcgta aaggtctgga
      421 taccttgacc tgcttttccc gtgtgcattc ccggctgacc ctgagcgaag tggcacgtct
      481 caccggctgt acccccgcta gcgctcgtcg ctccttgcgg accctctggc acttgggtta
      541 tctggagtgt gacgggaagc attactggat gaaccataag tgcttgctgg tcgcgaatgc
      601 ctatctcgct tcgcggccct tgcctagcct ggcgcaaccg ttgctcgatg gcctcagcga
      661 gcggacccgg gagagcgcta gcttggctaa attgctcgat ggtgaggcac tgattatcgc
      721 acggagcacg gctcggcgct ccctctcggt cgggctcggc attggctcgc ggttgcccgc
      781 ctattgttcc gcgctcggtc gggtcttgct ggcatcgttg ccacgctccg aggtgcgcgc
      841 tcgggtccgg cagatgaccc tccaggcgtt gacgccacgc accgtcacgg acgcaagcga
      901 ggtgatcgcc cgtgtcgacg aggcacgcga gcgtgggttc gcaacctcgg acggggaatt
      961 ggaaattggc gtgcgttcca tggcagtgcc cgtctttaac cgggcggggg cggtggtggc
     1021 gggcctgagc attgctgtgc gcgctgatcg catgggtctg tcggaattta tcgagggctt
     1081 tctgccaatg ctggggcggg cacgggaccg tctcggggag cagatctatc aggactaaca
     1141 gataaaaaaa atccttagct ttcgctaagg atgatttctg gctggtttaa ttaactaggt
     1201 tgacatggat ataatgtatg tacctttaac tttaagatag tcatatacat atgctcccgg
     1261 agtccaagcc gctgcagctc ccacagtttg tcgatgagcg tcgcgtgtcc ccttttcaat
     1321 atggggtcat catcctgtgt ggcttggtca tgttcctgga tggcttcgat acccaagcaa
     1381 ttagctatat ggcgccttat atcgcgcagg aatggtccct ctcgaagcag atgttgggcc
     1441 caattttctc cagctccttg gctggtctga tggtcgggta cctcgtcctg acgcctttgt
     1501 cggagcgttt tgggcataag aaggccatta ttgtgtcgac gatcgtgttt tccctctgca
     1561 ccctcgcttc ggtgtgggca acgaacgtca cggaattgat ggtcttgcgg ttcattacgg
     1621 gtatcgggtt gggcacggcc gccccttcgg caatcgcaat gaccggtgag tactccccga
     1681 agcggctgcg cgctaccttc gtcctggcaa tctattgcgg tttctccctg ggcttcatcg
     1741 cagcagggtt ggccgcaggc tggtttatcc cgcattatgg ttggcggtcg atgttttggg
     1801 tcggtgctct ggccccgctg atcttggtgc ccgtcctgtt tcgttttctc ccagagtcga
     1861 tggtgtttat gatcaaaaat aaggtccccc cacaacaaat cttgaacgtc tttcgtaaga
     1921 tcgatgcaag cgtctcgcag cagagcaaac ctgtcttcgt ggtcgaagca ctcgaacagg
     1981 gccaacacgg cgctctcgcg agcctgttca cccgtgaccg catcatgggt acggtgctgt
     2041 tgtggttggt gttcgcaatc aacctgggtg aattttatgc cctccagtcg tggctgccga
     2101 gcatcatgac ggggttgaat tacccgatga gcacggtcgt catggcgacc accttgacca
     2161 cggtcggggg tattgcggca gctttcatta cggggccaag catggaccgc ttgggtgcct
     2221 ataaaaccgt gggtattctc tacttggtcg gcttcgtctt tgtggccctg accggcatgg
     2281 catttaactc gccgttgtgg gtcttgttga gcgctaattt tctggctggg tgctgcattt
     2341 cgggtggtca gaaaagcctc attgcactcg ctgcagtgtt ctacccggcg ccaatgcggt
     2401 ccaccggtgt cggttgggct ctgggtattg gccggatcgg cgggatcgcg gggcctattg
     2461 tcgtgggcgc agctttgggt attggctggt cgccgagcgc ggtgttttac gggatggcaa
     2521 tcccaatgct gattgccggt ttgatcgtgc tctttctcgg tcgccgctat gggaactccg
     2581 ataaggtgta aggcctaggc ggcctcctgt gtgaaattgt tatccgcttt aattaaaggc
     2641 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2701 cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacagctggg cgcgcccccc
     2761 ctacgggctt gctctccggg cttcgccctg cgcggtcgct gcgctccctt gccagcccgt
     2821 ggatatgtgg acgatggccg cgagcggcca ccggctggct cgcttcgctc ggcccgtgga
     2881 caaccctgct ggacaagctg atggacaggc tgcgcctgcc cacgagcttg accacaggga
     2941 ttgcccaccg gctacccagc cttcgaccac atacccaccg gctccaactg cgcggcctgc
     3001 ggccttgccc catcaatttt tttaattttc tctggggaaa agcctccggc ctgcggcctg
     3061 cgcgcttcgc ttgccggttg gacaccaagt ggaaggcggg tcaaggctcg cgcagcgacc
     3121 gcgcagcggc ttggccttga cgcgcctgga acgacccaag cctatgcgag tgggggcagt
     3181 cgaagggcga agcccgcccg cctgcccccc gagcctcacg gcggcgagtg cgggggttcc
     3241 aagggggcag cgccaccttg ggcaaggccg aaggccgcgc agtcgatcaa caagccccgg
     3301 aggggccact ttttgccgga gggggagccg cgccgaaggc gtgggggaac cccgcagggg
     3361 tgcccttctt tgggcaccaa agaactagat atagggcgaa atgcgaaaga cttaaaaatc
     3421 aacaacttaa aaaagggggg tacgcaacag ctcattgcgg caccccccgc aatagctcat
     3481 tgcgtaggtt aaagaaaatc tgtaattgac tgccactttt acgcaacgca taattgttgt
     3541 cgcgctgccg aaaagttgca gctgattgcg catggtgccg caaccgtgcg gcacccctac
     3601 cgcatggaga taagcatggc cacgcagtcc agagaaatcg gcattcaagc caagaacaag
     3661 cccggtcact gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg gcttattgcg
     3721 aggaaaccca cggcggcaat gctgctgcat cacctcgtgg cgcagatggg ccaccagaac
     3781 gccgtggtgg tcagccagaa gacactttcc aagctcatcg gacgttcttt gcggacggtc
     3841 caatacgcag tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa gctcaacggc
     3901 cccggcaccg tgtcggccta cgtggtcaat gaccgcgtgg cgtggggcca gccccgcgac
     3961 cagttgcgcc tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga ccaggacgaa
     4021 tcgctgttgg ggcatggcga cctgcgccgc atcccgaccc tgtatccggg cgagcagcaa
     4081 ctaccgaccg gccccggcga ggagccgccc agccagcccg gcattccggg catggaacca
     4141 gacctgccag ccttgaccga aacggaggaa tgggaacggc gcgggcagca gcgcctgccg
     4201 atgcccgatg agccgtgttt tctggacgat ggcgagccgt tggagccgcc gacacgggta
     4261 acgctgccgc gccggtaggg ccggcctacg gccagcctcg cagagcagga ttcccgttga
     4321 gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta
     4381 cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc
     4441 tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa
     4501 tgaccccgaa gcagggttat gcagcggaaa aggacaaggg tcgtccaaaa aaaaaggctc
     4561 caaaaggagc ctttaattgt atcggtttac caatgtttaa tcaggctcgc gccaatttcc
     4621 gcaatctgac ggttacgttc atccatcgtc gcctggctgc cggtggtata aatcaccaca
     4681 atacggctcg gtttaccatc cgggcccagc gccgcaataa tgccacggct gccacgttcg
     4741 cccgcaccgc ttttatccgc aataaaccag ccagccggca gcgcgctacg cagcagcgga
     4801 cccgccactt tatccgcttc catccaatca atcagttgct ggcggcttgc cagggtcagc
     4861 agctcacccg tcagcagttt acgcagggtg gtcgccattg ctgccggcat ggtggtatca
     4921 cgttcatcgt tcggaatcgc ttcgttcagt tccggttccc aacgatccag acgggtcaca
     4981 tgatcgccca tgttatgcag aaacgcggtc agttctttcg gaccgccaat ggtcgtcagc
     5041 agcaggttcg ccgcggtgtt atcgctcatg gtaatcgccg cgctgcacag ttcacgcacg
     5101 gtcatgccat cggtcagatg tttttcggtc accgggctat attccaccag atcgttctgg
     5161 ctataatgaa tacgacggcc cagttgttcc tggcccgcat ccacacggct cagaaccgca
     5221 ccgcacagca gcactttaaa ggtgctcatc atcggaaaac gttcttccgg acgaaaagat
     5281 tccagaattt tgccgctgtt cagatccagt tcaatatagc ccacgcgcgc acccagttga
     5341 tcttccgcat ctttcacttt caccagggtt tccggatgcg caaacaccgg caggcaaaac
     5401 gccgcaaaaa acggaatcag cgccacacga aaatgctgaa tgctcatact cttccttttt
     5461 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5521 atttagaaaa ataaacaaat aggggaatta aaaaaaagcc cgctcattag gcgggctact
     5581 aatttaaatc gtaattattg gggacccctg gattctcacc aataaaaaac gcccggcggc
     5641 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     5701 ggagtccaag actagtcgcc agggttttcc cagtcacgac gcggccgctt atttatacag
     5761 ttcatccata ccatgggtaa tacctgctgc ggtaacaaat tccagcagaa ccatgtgatc
     5821 acgtttttcg ttcggatctt tgctcagaac gctctgggtg ctcagataat gattatccgg
     5881 cagcagcacc ggaccatcac caatcggggt attctgctga taatgatctg ccagctgaac
     5941 gctaccatct tccacattat gccgaatttt gaagttggct ttgatgccat ttttctgttt
     6001 gtctgcggtg atatacacgt tgtggctatt gaaattatat tccagtttgt ggcccaggat
     6061 attgccatct tctttaaaat cgatgccttt cagttcaatg cgattcacca gggtatcacc
     6121 ttcaaattta acttcggcac gggttttata ggtgccatca tctttgaagc tgatggtacg
     6181 ttcttgaaca taaccttccg gcattgcgct tttgaaaaaa tcgtggcgtt tcatgtgatc
     6241 cggataacgg ctaaaacact gaacaccata ggtcagggtg gtaaccaggg tcggccacgg
     6301 aaccggcagt ttgccggtgg tacagataaa tttcagggtc agtttaccat tggttgcatc
     6361 accttcgcct tcaccacgaa ctgaaaattt gtggccatta acatcaccat ccagttcaac
     6421 cagaatcgga acaacaccgg taaacagttc ttcacctttg ctcatatgta tatgactatc
     6481 ttaaagttaa aggggccttt gaagtatagt cgccggtttg acc
//
