LOCUS       LR-CR(QUAS:mNeon        8323 bp    DNA     circular     25-JUN-2025
DEFINITION  attB1-attB2-attB3-attB4.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-16030674|
COMMENT     VNTDBDATE|-16030674|
COMMENT     LSOWNER|
COMMENT     VNTNAME|LR-CR(QUAS:mNeonGreen-NES-P2A-mKate2-NLS)|
COMMENT     VNTAUTHORNAME|Enyedi Balázs|
COMMENT     VNTAUTHOREML|enyedib@eok.sote.hu|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "LR-CR(QUAS:mNeonGreen-NES-P2A-mKate2-NLS)" 0 8323 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 121 0 "" 0 "" "" "" "" "" "" "pDestTol2CR" "" 0 0 1 
COMMENT             6156 1 6156 2788 1104 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 2788 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 1104 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 163251064 0 "" 0 "" "" "" "" "" "" 
COMMENT             "p5E-QUAS_(L4-R1)" "" 0 0 1 212 6157 6368 1327 1538 0 "" "" "" "" 
COMMENT             "" "" (CObList) (CTerm 2 0 1327 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 1538 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 161776912 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pDONR221 mNeonGreen-NES-noSTOP" "" 0 0 1 801 6369 7169 2 802 0 "" 
COMMENT             "" "" "" "" "" (CObList) (CTerm 2 0 2 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 802 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #3=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "p3E_P2A-mKate2-3NLS_pA" 
COMMENT             "" 0 0 1 1154 7170 8323 735 1888 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 735 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 1888 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "Acc65I" "AflII" "AgeI" "ApaI" "ApaLI" "AseI" "AvaII" 
COMMENT         "BamHI" "BbsI" "BclI" "BglII" "BsaI" "BsmBI" "BspEI" "BsrGI" "BstBI" 
COMMENT         "BstXI" "Bsu36I" "ClaI" "DpnI" "EcoRI" "EcoRV" "HindIII" "KpnI" "MfeI" 
COMMENT         "MluI" "NcoI" "NdeI" "NheI" "NotI" "NruI" "PacI" "PmeI" "PspOMI" "PstI" 
COMMENT         "PvuI" "PvuII" "SacI" "SalI" "SmaI" "SpeI" "XbaI" "XhoI") 
COMMENT        (CStringList "atg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 1 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 8323 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #4=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 2 39 0 8064 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 2735 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 6396 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "ApaI" "gggccc" 6 0 3 564 0 6107 0 8076 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 1263 0 3563 0 4809 0 
COMMENT            7329 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AseI" "attaat" 3 0 4 1685 0 3020 0 3079 0 4314 
COMMENT            0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 9 2450 0 4280 0 4502 0 
COMMENT             5309 0 5445 0 6597 0 6814 0 6953 0 7246 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 4 6294 0 6390 0 7186 0 
COMMENT             8079 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "BbsI" "gaagac" 9 13 9 340 1 741 1 809 1 1042 
COMMENT             1 6359 0 6871 0 7550 0 7783 0 7851 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "BclI" "tgatca" 2 0 1 542 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "BglII" "agatct" 2 0 3 304 0 2260 0 7957 0 0 
COMMENT             "") 
COMMENT        #15=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 6 722 0 911 0 4203 1 7151 
COMMENT             1 7681 1 7870 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 6311 0 7220 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "BspEI" "tccgga" 2 0 2 641 0 7189 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 6 1300 0 2003 0 6368 0 
COMMENT             7109 0 7172 0 7292 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 7975 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 5 591 0 1555 0 1963 
COMMENT             0 2847 0 6978 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 3 1085 0 1988 0 7508 0 
COMMENT             0 "") #22=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 46 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "DpnI" "gatc" 3 0 40 55 0 292 0 301 0 306 0 
COMMENT             544 0 805 0 831 0 981 0 2262 0 2293 0 2588 0 2817 0 3817 0 3892 0 
COMMENT             3903 0 3911 0 3989 0 4001 0 4106 0 4447 0 4465 0 4511 0 4769 0 4786 
COMMENT             0 4822 0 5483 0 6296 0 6392 0 7145 0 7188 0 7615 0 7765 0 7791 0 
COMMENT             7959 0 7991 0 8015 0 8039 0 8081 0 8090 0 8121 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 605 0 6336 0 7977 0 0 
COMMENT             "") #25=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 597 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "HindIII" "aagctt" 2 0 3 2253 0 5764 0 7970 0 
COMMENT             0 "") 
COMMENT        #27=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 2 43 0 8068 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "MfeI" "caattg" 2 0 6 197 0 1442 0 1818 0 2548 
COMMENT             0 5336 0 8263 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 6356 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 1351 0 6407 0 6668 0 0 
COMMENT             "") #31=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 578 0 2834 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "NruI" "tcgcga" 4 0 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 1 555 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "PspOMI" "gggccc" 2 0 3 560 0 6103 0 8072 0 0 
COMMENT             "") 
COMMENT        #38=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 604 0 1467 0 5981 0 7124 
COMMENT             0 0 "") 
COMMENT        #39=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 4512 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 2327 0 3073 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "SacI" "gagctc" 6 0 6 2207 0 2855 0 6326 0 
COMMENT             7034 0 7055 0 7968 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 6271 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "SmaI" "cccggg" 4 0 2 7984 0 8077 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 2821 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "XbaI" "tctaga" 2 0 5 296 0 566 0 2827 0 6277 
COMMENT             0 8091 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 4 572 0 6108 0 7154 0 7961 
COMMENT             0 0 "")) 
COMMENT       (CObList 
COMMENT        #47=(CFSignal (CObList) "attB1" 21 0 0 6361 6381 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #48=(CFSignal (CObList) "attB2" 21 0 1 7162 7182 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #49=(CFSignal (CObList) "attB3" 21 0 1 8316 13 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #50=(CFSignal (CObList) "attB4" 21 0 0 6149 6169 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #51=(CFSignal (CObList) "MCS" 21 0 1 551 603 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3338..3390)") 
COMMENT        #52=(CFSignal (CObList) "BGHpA" 21 0 1 309 533 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3096..3320)") 
COMMENT        #53=(CFSignal (CObList) "insertion" 21 0 1 1336 1344 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4123..4131)") 
COMMENT        #54=(CFSignal (CObList) "mKate2" 21 0 1 646 1353 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3433..4140)") 
COMMENT        #55=(CFSignal (CObList) "cmlc2 promoter" 21 0 1 1360 2258 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4147..5045)") 
COMMENT        #56=(CFSignal (CObList) "medaka tyrosinase ex5 frag" 88 0 1 2782 2818 1 
COMMENT             (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#006600" 
COMMENT              "/ApEinfo_revcolor=#006600") 1 1 1 1 "complement(5569..5605)") 
COMMENT        #57=(CFSignal (CObList) "Tol2 exon4 136-482" 88 0 1 5755 6101 1 
COMMENT             (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#aa0000" 
COMMENT              "/ApEinfo_revcolor=#aa0000") 1 1 1 1 "complement(703..1049)") 
COMMENT        #58=(CFSignal (CObList) "Tol2 exon1 bp1-335 of 461" 88 0 1 2265 2599 1 
COMMENT             (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#aa0000" 
COMMENT              "/ApEinfo_revcolor=#aa0000") 1 1 1 1 "complement(5052..5386)") 
COMMENT        #59=(CFSignal (CObList) "Tol2 5' TIR" 19 0 1 2763 2781 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#ff0000" 
COMMENT              "/ApEinfo_revcolor=#ff0000") 1 1 1 1 "complement(5550..5568)") 
COMMENT        #60=(CFSignal (CObList) "Tol2 3' TIR" 19 0 1 5566 5582 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#ff0000" 
COMMENT              "/ApEinfo_revcolor=#ff0000") 1 1 1 1 "complement(514..530)") 
COMMENT        #61=(CFSignal (CObList) "T3" 30 0 1 2867 2886 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#777777" 
COMMENT              "/ApEinfo_revcolor=#777777") 1 1 1 1 "complement(5654..5673)") 
COMMENT        #62=(CFSignal (CObList) "M13F" 28 0 1 22 37 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#777777" 
COMMENT              "/ApEinfo_revcolor=#777777") 1 1 1 1 "complement(2809..2824)") 
COMMENT        #63=(CFSignal (CObList) "M13R(1)" 28 0 1 2902 2923 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_label=M13R" "/ApEinfo_fwdcolor=#777777" 
COMMENT              "/ApEinfo_revcolor=#777777") 1 1 1 1 "complement(5689..5710)") 
COMMENT        #64=(CFSignal (CObList) "M13R" 28 0 0 6113 6134 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#777777" 
COMMENT              "/ApEinfo_revcolor=#777777") 1 1 1 1 "1061..1082") 
COMMENT        #65=(CFSignal (CObList) "ampR" 4 0 1 4071 4928 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#ff7f00" 
COMMENT              "/ApEinfo_revcolor=#ff7f00") 1 1 1 1 "complement(6858..7715)") 
COMMENT        #66=(CFSignal (CObList) "colE1 ori" 33 0 1 3291 3973 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#999999" 
COMMENT              "/ApEinfo_revcolor=#999999") 1 1 1 1 "complement(6078..6760)") 
COMMENT        #67=(CFSignal (CObList) "pUC ori" 33 0 1 3250 3923 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#999999" 
COMMENT              "/ApEinfo_revcolor=#999999") 1 1 1 1 "complement(6037..6710)") 
COMMENT        #68=(CFSignal (CObList) "SV40 late polyA (from pCS2+)" 25 0 0 53 250 1 
COMMENT             (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=#ffff7f" 
COMMENT              "/ApEinfo_revcolor=#ffff7f") 1 1 1 1 "2840..3037") 
COMMENT        #69=(CFSignal (CObList) "CEBP_site" 21 0 0 6168 6176 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_revcolor=#f58a5e" 
COMMENT              "/ApEinfo_fwdcolor=#f58a5e") 1 1 1 1 "1338..1346") 
COMMENT        #70=(CFSignal (CObList) "QUAS" 21 0 0 6188 6203 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_revcolor=#d6b295" 
COMMENT              "/ApEinfo_fwdcolor=#d6b295") 1 1 1 1 "1358..1373") 
COMMENT        #71=(CFSignal (CObList) "QUAS" 21 0 0 6170 6185 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_revcolor=#faac61" 
COMMENT              "/ApEinfo_fwdcolor=#faac61") 1 1 1 1 "1340..1355") 
COMMENT        #72=(CFSignal (CObList) "QUAS" 21 0 0 6228 6243 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_revcolor=#faac61" 
COMMENT              "/ApEinfo_fwdcolor=#faac61") 1 1 1 1 "1398..1413") 
COMMENT        #73=(CFSignal (CObList) "QUAS" 21 0 0 6246 6261 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_revcolor=#faac61" 
COMMENT              "/ApEinfo_fwdcolor=#faac61") 1 1 1 1 "1416..1431") 
COMMENT        #74=(CFSignal (CObList) "QUAS" 21 0 0 6208 6223 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_revcolor=#faac61" 
COMMENT              "/ApEinfo_fwdcolor=#faac61") 1 1 1 1 "1378..1393") 
COMMENT        #75=(CFSignal (CObList) "NES" 21 0 0 7119 7148 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "752..781") 
COMMENT        #76=(CFSignal (CObList) "mNeonGreen" 21 0 0 6408 7115 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "41..748") 
COMMENT        #77=(CFSignal (CObList) "mKate2" 21 0 0 7251 7946 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "816..1511") 
COMMENT        #78=(CFSignal (CObList) "3NLS" 21 0 0 7989 8060 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1554..1625") 
COMMENT        #79=(CFSignal (CObList) "P2A" 21 0 0 7185 7250 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "750..815")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #80=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
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COMMENT                    "LR-CR(QUAS:mNeonGreen-NES-P2A-mKate2-NLS)" 2) 5 "" 0 4) 
COMMENT              #82=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
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COMMENT                          "DNA 'LR-CR(QUAS:mNeonGreen-NES-P2A-mKate2-NLS)'" 1) 
COMMENT                     #84=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "attB1-attB2-attB3-attB4." 1) 
COMMENT                     #85=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #86=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 06/25/25 09:09de." 1) 
COMMENT                     #87=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/25/25 09:09de." 1) 
COMMENT                     #88=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8323 bp" 1) 
COMMENT                     #89=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                     #90=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #93=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 24-JUN-2025" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                            #100=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
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COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/pDestTol2CR?sel=2788-1104\">pDestTol2CR</a> ") 
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COMMENT                           "#2: FRAGMENT of <a href=\"vnti:dnarna/p5E-QUAS_%28L4-R1%29?sel=1327-1538\">p5E-QUAS_(L4-R1)</a> ") 
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COMMENT                           "#3: FRAGMENT of <a href=\"vnti:dnarna/pDONR221%20mNeonGreen-NES-noSTOP?sel=2-802\">pDONR221 mNeonGreen-NES-noSTOP</a> ") 
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COMMENT                                    "M13R"))) "Primer Binding Site (3 total)") 
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COMMENT                                    "T3"))) "Promoter Prokaryotic (1 total)") 
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COMMENT                                    "pUC ori") 
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COMMENT                                    "colE1 ori"))) 
COMMENT                            "Replication Origin (2 total)") 
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COMMENT                                    "Tol2 exon1 bp1-335 of 461") 
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COMMENT                                    "medaka tyrosinase ex5 frag") 
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COMMENT                                            "Original Location Description:" 1) 
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COMMENT                                    "Tol2 exon4 136-482"))) 
COMMENT                            "Misc. Structure (3 total)"))) "Feature Map") 
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COMMENT                                    "Gene_1"))) "Gene (1 total)"))) 
COMMENT                    "Imported Features Not Shown on Map") 
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COMMENT                     (CObjectList 
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COMMENT                                            " N1: 39 " 1) 
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COMMENT                                            " N2: 8064 " 1))))) 
COMMENT                             "Acc65I: 2 sites") 1 5 "GGTACC" "CCATGG") 
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COMMENT                            1 5 "CTTAAG" "GAATTC") 
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COMMENT                            1 5 "ACCGGT" "TGGCCA") 
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COMMENT                                            " N1: 564 " 1) 
COMMENT                                      #343=(CLinePar 
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COMMENT                                      #344=(CLinePar 
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COMMENT                                            " N3: 8076 " 1))))) "ApaI: 3 sites") 
COMMENT                            5 1 "GGGCCC" "CCCGGG") 
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COMMENT                                            " N1: 1263 " 1) 
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COMMENT                                            " N2: 3563 " 1) 
COMMENT                                      #349=(CLinePar 
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COMMENT                                            " N3: 4809 " 1) 
COMMENT                                      #350=(CLinePar 
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COMMENT                                            " N4: 7329 " 1))))) "ApaLI: 4 sites") 
COMMENT                            1 5 "GTGCAC" "CACGTG") 
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COMMENT                                            " N1: 1685 " 1) 
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COMMENT                                      #355=(CLinePar 
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COMMENT                                            " N3: 3079 " 1) 
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COMMENT                                            " N4: 4314 " 1))))) "AseI: 4 sites") 
COMMENT                            2 4 "ATTAAT" "TAATTA") 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 2450 " 1) 
COMMENT                                      #360=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4280 " 1) 
COMMENT                                      #361=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4502 " 1) 
COMMENT                                      #362=(CLinePar 
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COMMENT                                            " N4: 5309 " 1) 
COMMENT                                      #363=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 5445 " 1) 
COMMENT                                      #364=(CLinePar 
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COMMENT                                            " N6: 6597 " 1) 
COMMENT                                      #365=(CLinePar 
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COMMENT                                            " N7: 6814 " 1) 
COMMENT                                      #366=(CLinePar 
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COMMENT                                            " N8: 6953 " 1) 
COMMENT                                      #367=(CLinePar 
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COMMENT                                            " N9: 7246 " 1))))) "AvaII: 9 sites") 
COMMENT                            1 4 "GGWCC" "CCWGG") 
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COMMENT                              (CObjectList 
COMMENT                               #369=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #11# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #11# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 6294 " 1) 
COMMENT                                      #371=(CLinePar 
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COMMENT                                            " N2: 6390 " 1) 
COMMENT                                      #372=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 7186 " 1) 
COMMENT                                      #373=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 8079 " 1))))) "BamHI: 4 sites") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
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COMMENT                                     (CObjectList 
COMMENT                                      #376=(CLinePar 
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COMMENT                                            " N1: 340 (on complementary strand)" 
COMMENT                                            1) 
COMMENT                                      #377=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 741 (on complementary strand)" 
COMMENT                                            1) 
COMMENT                                      #378=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 809 (on complementary strand)" 
COMMENT                                            1) 
COMMENT                                      #379=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 1042 (on complementary strand)" 
COMMENT                                            1) 
COMMENT                                      #380=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 6359 " 1) 
COMMENT                                      #381=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 6871 " 1) 
COMMENT                                      #382=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 7) 1 2 2 0 191) 
COMMENT                                            " N7: 7550 " 1) 
COMMENT                                      #383=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 8) 1 2 2 0 191) 
COMMENT                                            " N8: 7783 " 1) 
COMMENT                                      #384=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 9) 1 2 2 0 191) 
COMMENT                                            " N9: 7851 " 1))))) "BbsI: 9 sites") 
COMMENT                            8 12 "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #387=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 542 " 1))))) "BclI: 1 site") 1 
COMMENT                            5 "TGATCA" "ACTAGT") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 45836488 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #389=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #14# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #14# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 304 " 1) 
COMMENT                                      #391=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2260 " 1) 
COMMENT                                      #392=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 7957 " 1))))) "BglII: 3 sites") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 45836904 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #394=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #15# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #395=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 722 " 1) 
COMMENT                                      #396=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 911 " 1) 
COMMENT                                      #397=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4203 (on complementary strand)" 
COMMENT                                            1) 
COMMENT                                      #398=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 7151 (on complementary strand)" 
COMMENT                                            1) 
COMMENT                                      #399=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 7681 (on complementary strand)" 
COMMENT                                            1) 
COMMENT                                      #400=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 7870 (on complementary strand)" 
COMMENT                                            1))))) "BsaI: 6 sites") 7 11 
COMMENT                            "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 45837256 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #402=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #16# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #403=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #16# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 6311 " 1) 
COMMENT                                      #404=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #16# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 7220 (on complementary strand)" 
COMMENT                                            1))))) "BsmBI: 2 sites") 7 11 
COMMENT                            "CGTCTCNNNNNN" "GCAGAGNNNNNN") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 45836968 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #406=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #17# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #407=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 641 " 1) 
COMMENT                                      #408=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 7189 " 1))))) "BspEI: 2 sites") 
COMMENT                            1 5 "TCCGGA" "AGGCCT") 
COMMENT                      #409=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 45837512 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #410=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #18# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #411=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1300 " 1) 
COMMENT                                      #412=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2003 " 1) 
COMMENT                                      #413=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 6368 " 1) 
COMMENT                                      #414=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 7109 " 1) 
COMMENT                                      #415=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 7172 " 1) 
COMMENT                                      #416=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 7292 " 1))))) "BsrGI: 6 sites") 
COMMENT                            1 5 "TGTACA" "ACATGT") 
COMMENT                      #417=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 45836936 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #418=(CGroupPar 
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COMMENT                                     (CObjectList 
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COMMENT                            2 4 "TTCGAA" "AAGCTT") 
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COMMENT                            8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
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COMMENT                            4 "ATCGAT" "TAGCTA") 
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COMMENT                                      #463=(CLinePar 
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COMMENT                                      #464=(CLinePar 
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COMMENT                                      #465=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 29) 1 2 2 0 
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COMMENT                                      #466=(CLinePar 
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COMMENT                                      #476=(CLinePar 
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COMMENT                             "DpnI: 40 sites") 2 2 "GATC" "CTAG") 
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COMMENT                            1 5 "GAATTC" "CTTAAG") 
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COMMENT                            3 3 "GATATC" "CTATAG") 
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COMMENT                             "HindIII: 3 sites") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                            5 1 "GGTACC" "CCATGG") 
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COMMENT                                      #501=(CLinePar 
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COMMENT                            1 5 "CAATTG" "GTTAAC") 
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COMMENT                            1 5 "ACGCGT" "TGCGCA") 
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COMMENT                            1 5 "CCATGG" "GGTACC") 
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COMMENT                            "GTATAC") 
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COMMENT                            "CGATCG") 
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COMMENT                                      #515=(CLinePar 
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COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                            "AGCGCT") 
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COMMENT                            "AATTAATT") 
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COMMENT                            4 "GTTTAAAC" "CAAATTTG") 
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COMMENT                                            " N1: 560 " 1) 
COMMENT                                      #524=(CLinePar 
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COMMENT                                            " N2: 6103 " 1) 
COMMENT                                      #525=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #37# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 8072 " 1))))) 
COMMENT                             "PspOMI: 3 sites") 1 5 "GGGCCC" "CCCGGG") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 45837672 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 604 " 1) 
COMMENT                                      #529=(CLinePar 
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COMMENT                                            " N2: 1467 " 1) 
COMMENT                                      #530=(CLinePar 
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COMMENT                                            " N3: 5981 " 1) 
COMMENT                                      #531=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #38# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 7124 " 1))))) "PstI: 4 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
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COMMENT                                     (CObjectList 
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COMMENT                            4 2 "CGATCG" "GCTAGC") 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #40# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2327 " 1) 
COMMENT                                      #538=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #40# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3073 " 1))))) "PvuII: 2 sites") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #541=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2207 " 1) 
COMMENT                                      #542=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2855 " 1) 
COMMENT                                      #543=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 6326 " 1) 
COMMENT                                      #544=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 7034 " 1) 
COMMENT                                      #545=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 7055 " 1) 
COMMENT                                      #546=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 7968 " 1))))) "SacI: 6 sites") 
COMMENT                            5 1 "GAGCTC" "CTCGAG") 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 6271 " 1))))) "SalI: 1 site") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 7984 " 1) 
COMMENT                                      #553=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #43# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 8077 " 1))))) "SmaI: 2 sites") 
COMMENT                            3 3 "CCCGGG" "GGGCCC") 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                            1 5 "ACTAGT" "TGATCA") 
COMMENT                      #557=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #45# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 296 " 1) 
COMMENT                                      #560=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #45# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 566 " 1) 
COMMENT                                      #561=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #45# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2827 " 1) 
COMMENT                                      #562=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #45# 4) 1 2 2 0 191) 
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FEATURES             Location/Qualifiers
     misc_feature    6361..6381
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(7162..7182)
                     /vntifkey="21"
                     /label=attB2
     misc_feature    complement(8316..13)
                     /vntifkey="21"
                     /label=attB3
     misc_feature    6149..6169
                     /vntifkey="21"
                     /label=attB4
     misc_feature    complement(551..603)
                     /vntifkey="21"
                     /label=MCS
     misc_feature    complement(309..533)
                     /vntifkey="21"
                     /label=BGHpA
     misc_feature    complement(1336..1344)
                     /vntifkey="21"
                     /label=insertion
     misc_feature    complement(646..1353)
                     /vntifkey="21"
                     /label=mKate2
     misc_feature    complement(1360..2258)
                     /vntifkey="21"
                     /label=cmlc2\promoter
     gene            5280..5566
                     /ApEinfo_fwdcolor=#006600
                     /ApEinfo_revcolor=#006600
                     /vntifkey="60"
     misc_structure  complement(2782..2818)
                     /ApEinfo_fwdcolor=#006600
                     /ApEinfo_revcolor=#006600
                     /vntifkey="88"
                     /label=medaka\tyrosinase\ex5\frag
     misc_structure  complement(5755..6101)
                     /ApEinfo_fwdcolor=#aa0000
                     /ApEinfo_revcolor=#aa0000
                     /vntifkey="88"
                     /label=Tol2\exon4\136-482
     misc_structure  complement(2265..2599)
                     /ApEinfo_fwdcolor=#aa0000
                     /ApEinfo_revcolor=#aa0000
                     /vntifkey="88"
                     /label=Tol2\exon1\bp1-335\of\461
     LTR             complement(2763..2781)
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /vntifkey="19"
                     /label=Tol2\5'\TIR
     LTR             complement(5566..5582)
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /vntifkey="19"
                     /label=Tol2\3'\TIR
     promoter        complement(2867..2886)
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="30"
                     /label=T3
     primer_bind     complement(22..37)
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13F
     primer_bind     complement(2902..2923)
                     /ApEinfo_label=M13R
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13R(1)
     primer_bind     6113..6134
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13R
     CDS             complement(4071..4928)
                     /ApEinfo_fwdcolor=#ff7f00
                     /ApEinfo_revcolor=#ff7f00
                     /vntifkey="4"
                     /label=ampR
     rep_origin      complement(3291..3973)
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
                     /vntifkey="33"
                     /label=colE1\ori
     rep_origin      complement(3250..3923)
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
                     /vntifkey="33"
                     /label=pUC\ori
     polyA_signal    53..250
                     /ApEinfo_fwdcolor=#ffff7f
                     /ApEinfo_revcolor=#ffff7f
                     /vntifkey="25"
                     /label=SV40\late\polyA\(from\pCS2+)
     misc_feature    6168..6176
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
                     /vntifkey="21"
                     /label=CEBP_site
     misc_feature    6188..6203
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
                     /vntifkey="21"
                     /label=QUAS
     misc_feature    6170..6185
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
                     /vntifkey="21"
                     /label=QUAS
     misc_feature    6228..6243
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
                     /vntifkey="21"
                     /label=QUAS
     misc_feature    6246..6261
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
                     /vntifkey="21"
                     /label=QUAS
     misc_feature    6208..6223
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
                     /vntifkey="21"
                     /label=QUAS
     misc_feature    7119..7148
                     /vntifkey="21"
                     /label=NES
     misc_feature    6408..7115
                     /vntifkey="21"
                     /label=mNeonGreen
     misc_feature    7251..7946
                     /vntifkey="21"
                     /label=mKate2
     misc_feature    7989..8060
                     /vntifkey="21"
                     /label=3NLS
     misc_feature    7185..7250
                     /vntifkey="21"
                     /label=P2A
BASE COUNT     2257 a      1965 c      1988 g      2113 t 
ORIGIN
        1 ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg atgatccaga 
       61 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg 
      121 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa 
      181 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga 
      241 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga 
      301 tcagatctcc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc 
      361 ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg 
      421 cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa 
      481 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc 
      541 tgatcagcgg gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct 
      601 gcagaattca cctgctcctc ctgctcccgc tgctgcgcct ccggacagtt tgtgccccag 
      661 tttgctaggg aggtcgcagt atctggccac agccacctcg tgctgctcga cgtaggtctc 
      721 tttgtcggcc tccttgattc tttccagtct tctgtccaca tagtagacgc cgggcatctt 
      781 gaggttctta gcgggtttct tggatctgta tgtggtcttc aagttgcaga tcaggtggcc 
      841 cccgcccacg agcttcaggg ccatgtcggc tctgccttcc aggccgccgt cagcggggta 
      901 cagggtctcg gtggaggcct cccagccgag tgttttcttc tgcatcacag ggccgttgga 
      961 tgggaagttc acccctctga tcttgacgtt gtagatgagg cagccgtcct ggaggctggt 
     1021 gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa 
     1081 gccctcaggg aaggactgct taaagaagtc ggggatgccc tgggtgtggt tgatgaaggt 
     1141 tttgctgccg tacatgaagc tggtagccag gatgtcgaag gcgaagggga gagggccgcc 
     1201 ctcgacggcc ttgattctca tggtctgggt gccctcgtag ggcttgcctt cgccctcgga 
     1261 tgtgcacttg aagtggtggt tgttcacggt gccctccatg tacagcttca tgtgcatgtt 
     1321 ctccttaatc agctcttcgc ccttagacac catggtggcg gtcactgtct gctttgctgt 
     1381 tggtctgggc tcctgggtca ctggcttact aatggagtct ttatgtatga ggactcttat 
     1441 caattgttct tctataaagg tctgcagtgt ttctgttcgt cccctacatg gacacccaga 
     1501 gcctcctaaa tacaggagcc ctgataactg cacaagtgct cagattccag cagggtggaa 
     1561 aatgagataa agtgtgcaga tggggagggg gacgtgaatg agagatttga gggatgaaaa 
     1621 ggatggatga acgcattgaa aatagcccct ttcacaaaat aataccagta aattgtcata 
     1681 taattaatga ccagaccttt acctgtaaat gatgtgatgt tcatatacag aagaatgcca 
     1741 acaatttaca gatgatttta caaattctta attttccttt tctaaatgga tttgttcaca 
     1801 cattatctct tcaaagcaat tgaattttct ggaaaagatt ggctgtgtgt gtaaaaggtg 
     1861 ctactgagga atttaatgtg acatggaatg aagcaaacag cagcctaaga caggatggca 
     1921 ggaaaaatgt cacatgttta aaataagagt gaaaccaacg acctggactt atagagactg 
     1981 tgtgtcctta ggcaaagctc ttgtacaaac agtgcttgtg actgtgaaat tcggggtttg 
     2041 cctggattgt gttaaatgtg tgcttggatg tatcataaaa aagaatcggt tctctgtatt 
     2101 tgacattttc tgtaatttat ctaaagttac aactggctat gcctgatttt tatcactatt 
     2161 gaatcaatca ttttaaagaa tgttaagtct gctgatttgg agagctcatt tacatttatt 
     2221 ttgaatgtct cttatttaac acaactgatt taagctttag atctgcgaag atacggccac 
     2281 gggtgctctt gatcctgtgg ctgattttgg actgtgctgc tcgcagctgc tgatgaatca 
     2341 catacttcct ccattttctt ccactgattg actgttataa tttccctaat ttccaggtca 
     2401 aggtgctgtg cattgtggta atagatgtga catgacgtca cttccaaagg accaatgaac 
     2461 atgtctgacc aatttcatat aatgtgaaaa cgattttcat aggcagaata aataacattt 
     2521 aaattaaact gggcatcagc gcaattcaat tggtttggta atagcaaggg aaaatagaat 
     2581 gaagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa 
     2641 gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat ttttaattgt 
     2701 actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca agtaaaatta 
     2761 ctcaagtact ttacacctct ggttcttgac cccctacctt cagcaagccc agcagatcca 
     2821 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg 
     2881 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 
     2941 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 
     3001 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 
     3061 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 
     3121 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     3181 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 
     3241 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     3301 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     3361 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     3421 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     3481 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 
     3541 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     3601 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     3661 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     3721 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 
     3781 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     3841 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     3901 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     3961 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     4021 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     4081 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     4141 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     4201 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     4261 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     4321 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     4381 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     4441 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     4501 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     4561 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     4621 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     4681 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     4741 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     4801 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     4861 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     4921 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     4981 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     5041 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt 
     5101 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 
     5161 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 
     5221 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 
     5281 gctggctaag aactcatcag cctccccggt ccatctaccc acgtaccaat gcaccaattg 
     5341 gccacaatga cggctactac atggtgccat tccttcctct ttataggaat ggagactacc 
     5401 tcctgtccaa caaggctctt ggatacgagt acgcctacct gttggaccca ggtcattgca 
     5461 caacaccaga aatgccctct gatctgcaaa agacgtgaat atctgttcag acacccatat 
     5521 ccactctgtt ccacacaggt cagaggtttg tccaggagtt cttgacagag gtgtaaaaag 
     5581 tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt actcaattaa 
     5641 aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac tccattaaaa 
     5701 ttgtacttga gtattaagga agtaaaagta aaagcaagaa agaaaactag agattcttgt 
     5761 ttaagctttt aatctcaaaa aacattaaat gaaatgcata caaggtttta tcctgcttta 
     5821 gaactgtttg tatttaatta tcaaactata agacagacaa tctaatgcca gtacacgcta 
     5881 ctcaaagttg taaaacctca gatttaactt cagtagaagc tgattctcaa aattgttagt 
     5941 gtcaagccta gctcttttgg ggctgaaaag caatcctgca gtgctgaaaa gcctctcaca 
     6001 ggcagccgat gcgggaagag gtgtattagt cttgatagag aggctgcaaa tagcaggaaa 
     6061 cgtgagcaga gactccctgg tgtctgaaac acaggccaga tgggccctcg agcaggaaac 
     6121 agctatgacc atgattacgc caagctatca actttgtata gaaaagttgg ggtaatcgct 
     6181 tatcctcgga taaacaatta tcctcacggg taatcgctta tccgctcggg taatcgctta 
     6241 tcctcgggta atcgcttatc cttgcaaggg tcgactctag agggtatata atggatccca 
     6301 tcgcgtctca gcctcacttt gagctcctcc acacgaattc cctcgacctc gaagacgcgt 
     6361 caagtttgta caaaaaagca ggctggacgg atccaccggt cgccaccatg gtgagcaagg 
     6421 gcgaggagga taacatggcc tctctcccag cgacacatga gttacacatc tttggctcca 
     6481 tcaacggtgt ggactttgac atggtgggtc agggcaccgg caatccaaat gatggttatg 
     6541 aggagttaaa cctgaagtcc accaagggtg acctccagtt ctccccctgg attctggtcc 
     6601 ctcatatcgg gtatggcttc catcagtacc tgccctaccc tgacgggatg tcgcctttcc 
     6661 aggccgccat ggtagatggc tccggctacc aagtccatcg cacaatgcag tttgaagatg 
     6721 gtgcctccct tactgttaac taccgctaca cctacgaggg aagccacatc aaaggagagg 
     6781 cccaggtgaa ggggactggt ttccctgctg acggtcctgt gatgaccaac tcgctgaccg 
     6841 ctgcggactg gtgcaggtcg aagaagactt accccaacga caaaaccatc atcagtacct 
     6901 ttaagtggag ttacaccact ggaaatggca agcgctaccg gagcactgcg cggaccacct 
     6961 acacctttgc caagccaatg gcggctaact atctgaagaa ccagccgatg tacgtgttcc 
     7021 gtaagacgga gctcaagcac tccaagaccg agctcaactt caaggagtgg caaaaggcct 
     7081 ttaccgatgt gatgggcatg gacgagctgt acaaggagct gcagaacaag ctggaagagt 
     7141 tggatctgga ctctcgagac ccagctttct tgtacaaagt ggggggatcc ggagccacga 
     7201 acttctctct gttaaagcaa gcaggagacg tggaagaaaa ccccggtcct atggtgtctg 
     7261 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc 
     7321 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa 
     7381 tcaaggccgt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca 
     7441 tgtacggcag caaaaccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt 
     7501 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga 
     7561 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag 
     7621 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct 
     7681 ccaccgagac cctgtacccc gctgacggcg gcctggaagg cagagccgac atggccctga 
     7741 agctcgtggg cgggggccac ctgatctgca acttgaagac cacatacaga tccaagaaac 
     7801 ccgctaagaa cctcaagatg cccggcgtct actatgtgga cagaagactg gaaagaatca 
     7861 aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg 
     7921 acctccctag caaactgggg cacagaggcg gtggaagatc tcgagctcaa gcttcgaatt 
     7981 cccgggtcga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtagatc 
     8041 caaaaaagaa gagaaaggta taggtaccgc gggcccggga tccaccggat ctagaactat 
     8101 agtgagtcgt attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc 
     8161 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 
     8221 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 
     8281 tttcaggttc agggggaggt gtgggaggtt ttttccaact tta 
//
