LOCUS       LR-CG(QUAS:cPLA2        9561 bp    DNA     circular      6-DEC-2019
DEFINITION  attB1-attB2-attB3-attB4.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13111288|
COMMENT     VNTDBDATE|-13111288|
COMMENT     LSOWNER|
COMMENT     VNTNAME|LR-CG(QUAS:cPLA2-mKate2:pA)|
COMMENT     VNTAUTHORNAME|Enyedi Balázs|
COMMENT     VNTAUTHOREML|enyedib@eok.sote.hu|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                                           "   /ApEinfo_fwdcolor=#006600" 1) 
COMMENT                                     #276=(CLinePar 
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COMMENT                                   "medaka tyrosinase ex5 frag") 
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COMMENT                                           "Start: 5712  End: 6058 (Complementary)" 
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COMMENT                                   "Tol2 exon4 136-482"))) 
COMMENT                           "Misc. Structure (4 total)"))) "Feature Map") 
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COMMENT                                    "Gene_1"))) "Gene (1 total)"))) 
COMMENT                    "Imported Features Not Shown on Map") 
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COMMENT                            1 5 "GGTACC" "CCATGG") 
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COMMENT                            "TGGCCA") 
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COMMENT                                            " N2: 4658 " 1) 
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COMMENT                                            " N3: 7808 " 1) 
COMMENT                                      #303=(CLinePar 
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COMMENT                                            " N4: 8701 " 1))))) "ApaLI: 4 sites") 
COMMENT                            1 5 "GTGCAC" "CACGTG") 
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COMMENT                                            " N1: 6251 " 1) 
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COMMENT                                            " N2: 6583 " 1) 
COMMENT                                      #308=(CLinePar 
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COMMENT                                            " N3: 9357 " 1))))) "BamHI: 3 sites") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
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COMMENT                                     (CObjectList 
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COMMENT                                      #313=(CLinePar 
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COMMENT                                            " N3: 9155 " 1) 
COMMENT                                      #314=(CLinePar 
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COMMENT                                            " N4: 9223 " 1))))) "BbsI: 4 sites") 
COMMENT                            8 12 "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 6799 " 1))))) "BclI: 1 site") 
COMMENT                            1 5 "TGATCA" "ACTAGT") 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 304 " 1) 
COMMENT                                      #321=(CLinePar 
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COMMENT                                            " N2: 2109 " 1))))) "BglII: 2 sites") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
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COMMENT                              (CObjectList 
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COMMENT                                            " N1: 7918 " 1) 
COMMENT                                      #325=(CLinePar 
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COMMENT                                            " N2: 8271 " 1))))) "BspEI: 2 sites") 
COMMENT                            1 5 "TCCGGA" "AGGCCT") 
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COMMENT                                            " N1: 495 " 1) 
COMMENT                                      #329=(CLinePar 
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COMMENT                                            " N2: 1852 " 1) 
COMMENT                                      #330=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 6325 " 1) 
COMMENT                                      #331=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 8664 " 1) 
COMMENT                                      #332=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 9344 " 1))))) "BsrGI: 5 sites") 
COMMENT                            1 5 "TGTACA" "ACATGT") 
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COMMENT                             (CGroupPar (CParagraph 75637920 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 1404 " 1) 
COMMENT                                      #336=(CLinePar 
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COMMENT                                            " N2: 1812 " 1) 
COMMENT                                      #337=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2696 " 1) 
COMMENT                                      #338=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 6604 " 1))))) "BstXI: 4 sites") 
COMMENT                            8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 46 " 1) 
COMMENT                                      #342=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6354 " 1))))) "ClaI: 2 sites") 
COMMENT                            2 4 "ATCGAT" "TAGCTA") 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 6293 " 1) 
COMMENT                                      #346=(CLinePar 
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COMMENT                                            " N2: 8598 " 1))))) "EcoRI: 2 sites") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75637344 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EcoRV: 0 sites") 3 3 "GATATC" 
COMMENT                            "CTATAG") 
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COMMENT                              (CObjectList 
COMMENT                               #349=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #350=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2102 " 1) 
COMMENT                                      #351=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5721 " 1) 
COMMENT                                      #352=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 6857 " 1))))) 
COMMENT                             "HindIII: 3 sites") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #355=(CLinePar 
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COMMENT                                            " N1: 43 " 1))))) "KpnI: 1 site") 5 
COMMENT                            1 "GGTACC" "CCATGG") 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 197 " 1) 
COMMENT                                      #359=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1291 " 1) 
COMMENT                                      #360=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1667 " 1) 
COMMENT                                      #361=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 2397 " 1) 
COMMENT                                      #362=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 5293 " 1) 
COMMENT                                      #363=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 9501 " 1))))) "MfeI: 6 sites") 
COMMENT                            1 5 "CAATTG" "GTTAAC") 
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COMMENT                              (CObjectList 
COMMENT                               #365=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #366=(CLinePar 
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COMMENT                                            " N1: 6313 " 1))))) "MluI: 1 site") 
COMMENT                            1 5 "ACGCGT" "TGCGCA") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75638048 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CParagraph 0 (10 #19# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #369=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #19# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1206 " 1))))) "NcoI: 1 site") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75638112 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #371=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #372=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #20# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7015 " 1))))) "NdeI: 1 site") 
COMMENT                            2 4 "CATATG" "GTATAC") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75638016 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
COMMENT                            "CGATCG") 
COMMENT                      #374=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
COMMENT                               #375=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #376=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #22# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2683 " 1))))) "NotI: 1 site") 
COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                      #377=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75638592 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #378=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #379=(CLinePar 
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COMMENT                                            " N1: 1316 " 1) 
COMMENT                                      #380=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5938 " 1) 
COMMENT                                      #381=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 7693 " 1))))) "PstI: 3 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
COMMENT                      #382=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75638944 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #383=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #31# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #384=(CLinePar 
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COMMENT                                            " N1: 4361 " 1))))) "PvuI: 1 site") 
COMMENT                            4 2 "CGATCG" "GCTAGC") 
COMMENT                      #385=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75638848 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #386=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #24# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #387=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #24# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2176 " 1) 
COMMENT                                      #388=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #24# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2922 " 1) 
COMMENT                                      #389=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #24# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 7731 " 1))))) "PvuII: 3 sites") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75638464 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #391=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #392=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #29# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2056 " 1) 
COMMENT                                      #393=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #29# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2704 " 1) 
COMMENT                                      #394=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #29# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 6283 " 1) 
COMMENT                                      #395=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #29# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 7113 " 1) 
COMMENT                                      #396=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #29# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 7289 " 1) 
COMMENT                                      #397=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #29# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 7371 " 1))))) "SacI: 6 sites") 
COMMENT                            5 1 "GAGCTC" "CTCGAG") 
COMMENT                      #398=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75638720 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #399=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #25# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #400=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #25# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 6228 " 1) 
COMMENT                                      #401=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #25# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 8039 " 1))))) "SalI: 2 sites") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
COMMENT                      #402=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75638208 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG" 
COMMENT                            "GGGCCC") 
COMMENT                      #403=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75638368 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #404=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #27# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #405=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #27# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 296 " 1) 
COMMENT                                      #406=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #27# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 466 " 1) 
COMMENT                                      #407=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #27# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2676 " 1) 
COMMENT                                      #408=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #27# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 6234 " 1))))) "XbaI: 4 sites") 
COMMENT                            1 5 "TCTAGA" "AGATCT") 
COMMENT                      #409=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 75638912 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #410=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #28# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #411=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #28# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 474 " 1) 
COMMENT                                      #412=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #28# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6065 " 1) 
COMMENT                                      #413=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #28# 3) 1 2 2 0 191) 
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FEATURES             Location/Qualifiers
     misc_feature    6318..6338
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(9334..9354)
                     /vntifkey="21"
                     /label=attB2
     misc_feature    complement(9554..13)
                     /vntifkey="21"
                     /label=attB3
     misc_feature    6106..6126
                     /vntifkey="21"
                     /label=attB4
     gene            5238..5523
                     /ApEinfo_fwdcolor=#006600
                     /ApEinfo_revcolor=#006600
                     /vntifkey="60"
     misc_structure  complement(2631..2667)
                     /ApEinfo_fwdcolor=#006600
                     /ApEinfo_revcolor=#006600
                     /vntifkey="88"
                     /label=medaka\tyrosinase\ex5\frag
     misc_structure  complement(5712..6058)
                     /ApEinfo_fwdcolor=#aa0000
                     /ApEinfo_revcolor=#aa0000
                     /vntifkey="88"
                     /label=Tol2\exon4\136-482
     misc_structure  complement(2114..2448)
                     /ApEinfo_fwdcolor=#aa0000
                     /ApEinfo_revcolor=#aa0000
                     /vntifkey="88"
                     /label=Tol2\exon1\bp1-335\of\461
     LTR             complement(2612..2630)
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /vntifkey="19"
                     /label=Tol2\5'\TIR
     LTR             complement(5523..5539)
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /vntifkey="19"
                     /label=Tol2\3'\TIR
     promoter        complement(2716..2735)
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="30"
                     /label=T3
     primer_bind     complement(22..37)
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13F
     primer_bind     complement(2751..2772)
                     /ApEinfo_label=M13R
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13R(1)
     primer_bind     6070..6091
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13R
     CDS             complement(3920..4777)
                     /ApEinfo_fwdcolor=#ff7f00
                     /ApEinfo_revcolor=#ff7f00
                     /vntifkey="4"
                     /label=ampR
     rep_origin      complement(3099..3772)
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
                     /vntifkey="33"
                     /label=pUC\ori
     misc_structure  complement(1004..1017)
                     /ApEinfo_fwdcolor=#00cc00
                     /ApEinfo_revcolor=#00cc00
                     /vntifkey="88"
                     /label=EGFP-like\chromophore
     CDS             complement(492..1208)
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /vntifkey="4"
                     /label=EGFP
     promoter        complement(1209..2107)
                     /ApEinfo_fwdcolor=#ffffbf
                     /ApEinfo_revcolor=#ffffbf
                     /vntifkey="30"
                     /label=cmlc2\promoter
     polyA_signal    complement(309..441)
                     /ApEinfo_label=SV40 late polyA (from pCS2+)
                     /ApEinfo_fwdcolor=#ffff7f
                     /ApEinfo_revcolor=#ffff7f
                     /vntifkey="25"
                     /label=SV40\late\polyA\(from\pCS2+)(1)
     polyA_signal    53..250
                     /ApEinfo_fwdcolor=#ffff7f
                     /ApEinfo_revcolor=#ffff7f
                     /vntifkey="25"
                     /label=SV40\late\polyA\(from\pCS2+)
     misc_feature    6125..6133
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
                     /vntifkey="21"
                     /label=CEBP_site
     misc_feature    6145..6160
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
                     /vntifkey="21"
                     /label=QUAS
     misc_feature    6127..6142
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
                     /vntifkey="21"
                     /label=QUAS
     misc_feature    6185..6200
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
                     /vntifkey="21"
                     /label=QUAS
     misc_feature    6203..6218
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
                     /vntifkey="21"
                     /label=QUAS
     misc_feature    6165..6180
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
                     /vntifkey="21"
                     /label=QUAS
     misc_feature    6564..6564
                     /vntifkey="21"
                     /label=PCR\mut\A-->G
     misc_feature    6364..8586
                     /vntifkey="21"
                     /label=cPLA2
     misc_feature    6364..9324
                     /vntifkey="21"
                     /label=cPLA2-mKate2
     misc_feature    8614..9321
                     /vntifkey="21"
                     /label=mKate2
     polyA_signal    9357..9552
                     /ApEinfo_fwdcolor=#ffff7f
                     /ApEinfo_revcolor=#ffff7f
                     /vntifkey="25"
                     /label=SV40\late\polyA\(from\pCS2+)
     misc_feature    9554..7
                     /ApEinfo_fwdcolor=#4259cc
                     /ApEinfo_revcolor=#4259cc
                     /vntifkey="21"
                     /label=att3_shared
BASE COUNT     2620 a      2216 c      2256 g      2469 t 
ORIGIN
        1 ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg atgatccaga 
       61 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg 
      121 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa 
      181 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga 
      241 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga 
      301 tcagatctct tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
      361 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
      421 gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga ggctcgagag 
      481 gggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac 
      541 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg 
      601 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg 
      661 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc 
      721 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac 
      781 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg 
      841 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg 
      901 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag 
      961 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg 
     1021 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc 
     1081 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt 
     1141 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg 
     1201 ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct cctgggtcac tggcttacta 
     1261 atggagtctt tatgtatgag gactcttatc aattgttctt ctataaaggt ctgcagtgtt 
     1321 tctgttcgtc ccctacatgg acacccagag cctcctaaat acaggagccc tgataactgc 
     1381 acaagtgctc agattccagc agggtggaaa atgagataaa gtgtgcagat ggggaggggg 
     1441 acgtgaatga gagatttgag ggatgaaaag gatggatgaa cgcattgaaa atagcccctt 
     1501 tcacaaaata ataccagtaa attgtcatat aattaatgac cagaccttta cctgtaaatg 
     1561 atgtgatgtt catatacaga agaatgccaa caatttacag atgattttac aaattcttaa 
     1621 ttttcctttt ctaaatggat ttgttcacac attatctctt caaagcaatt gaattttctg 
     1681 gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa tttaatgtga catggaatga 
     1741 agcaaacagc agcctaagac aggatggcag gaaaaatgtc acatgtttaa aataagagtg 
     1801 aaaccaacga cctggactta tagagactgt gtgtccttag gcaaagctct tgtacaaaca 
     1861 gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg ttaaatgtgt gcttggatgt 
     1921 atcataaaaa agaatcggtt ctctgtattt gacattttct gtaatttatc taaagttaca 
     1981 actggctatg cctgattttt atcactattg aatcaatcat tttaaagaat gttaagtctg 
     2041 ctgatttgga gagctcattt acatttattt tgaatgtctc ttatttaaca caactgattt 
     2101 aagctttaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga 
     2161 ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga 
     2221 ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac 
     2281 atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac 
     2341 gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt 
     2401 ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg 
     2461 actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag 
     2521 taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa 
     2581 tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc 
     2641 ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga 
     2701 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat 
     2761 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 
     2821 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 
     2881 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 
     2941 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 
     3001 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 
     3061 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
     3121 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     3181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     3241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     3301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     3361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     3421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     3481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     3541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 
     3601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     3661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     3721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     3781 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 
     3841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 
     3901 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 
     3961 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 
     4021 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 
     4081 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 
     4141 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 
     4201 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 
     4261 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 
     4321 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 
     4381 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 
     4441 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 
     4501 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 
     4561 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 
     4621 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 
     4681 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 
     4741 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 
     4801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 
     4861 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt 
     4921 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata 
     4981 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 
     5041 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 
     5101 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 
     5161 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 
     5221 ttgacgggga aagccgggct ggctaagaac tcatcagcct ccccggtcca tctacccacg 
     5281 taccaatgca ccaattggcc acaatgacgg ctactacatg gtgccattcc ttcctcttta 
     5341 taggaatgga gactacctcc tgtccaacaa ggctcttgga tacgagtacg cctacctgtt 
     5401 ggacccaggt cattgcacaa caccagaaat gccctctgat ctgcaaaaga cgtgaatatc 
     5461 tgttcagaca cccatatcca ctctgttcca cacaggtcag aggtttgtcc aggagttctt 
     5521 gacagaggtg taaaaagtac tcaaaaattt tactcaagtg aaagtacaag tacttaggga 
     5581 aaattttact caattaaaag taaaagtatc tggctagaat cttacttgag taaaagtaaa 
     5641 aaagtactcc attaaaattg tacttgagta ttaaggaagt aaaagtaaaa gcaagaaaga 
     5701 aaactagaga ttcttgttta agcttttaat ctcaaaaaac attaaatgaa atgcatacaa 
     5761 ggttttatcc tgctttagaa ctgtttgtat ttaattatca aactataaga cagacaatct 
     5821 aatgccagta cacgctactc aaagttgtaa aacctcagat ttaacttcag tagaagctga 
     5881 ttctcaaaat tgttagtgtc aagcctagct cttttggggc tgaaaagcaa tcctgcagtg 
     5941 ctgaaaagcc tctcacaggc agccgatgcg ggaagaggtg tattagtctt gatagagagg 
     6001 ctgcaaatag caggaaacgt gagcagagac tccctggtgt ctgaaacaca ggccagatgg 
     6061 gccctcgagc aggaaacagc tatgaccatg attacgccaa gctatcaact ttgtatagaa 
     6121 aagttggggt aatcgcttat cctcggataa acaattatcc tcacgggtaa tcgcttatcc 
     6181 gctcgggtaa tcgcttatcc tcgggtaatc gcttatcctt gcaagggtcg actctagagg 
     6241 gtatataatg gatcccatcg cgtctcagcc tcactttgag ctcctccaca cgaattccct 
     6301 cgacctcgaa gacgcgtcaa gtttgtacaa aaaagcaggc tggacggatc tatcgatgcc 
     6361 acaatgtcca acattatagt tgagcatcag tattctcata gactgaaatt gactgtggtg 
     6421 agagctgaaa atgtaactaa aggagcattt ggggatttat tggacacccc ggacccttat 
     6481 gttgagctgt cagtgccaac cacaccagag tcaagaaaac gaacccgcca cattaacaat 
     6541 gacattaacc ccaagtggaa cgagaccttt gagttcatat tggatcccaa tcaatccaat 
     6601 gttttggagg tgacactgat ggatgccaat tatgtcatgg atgaaactct tggcacagca 
     6661 aaatattccc tctcaaaact caaagtggca caaatggagc atgtgaccct ctctatcggc 
     6721 aagacaacca aagtgttcct cgacttgctc ttggaagttt gcgcatccac agacctgcgc 
     6781 ttcagcatga cactgtgtga tcaagagaag ctgttcatgc agacacgcag agacagggtc 
     6841 atgctcagca taaagaagct tcttaaaatg gaaaaccctc gtttccttcc atcctcacca 
     6901 agagaggttc ccaccatcgc tattctgggc tctggaggag gttttcgtgc aatggtgggc 
     6961 ttctctggtg tgatgaaggc tttgtatgaa tctggcgtgt ttgactgtgc aacatatgtg 
     7021 gcaggacttt caggatcaac atggtatatg tctatgctgt actctcatcc agagtttcct 
     7081 gctaaaggcc caggcgacat taataaagag ctcatgaaca gagtcagtaa taacccactc 
     7141 aaactgctcc tgccccaaaa catcaaccgc tacgtcaaag cactgtggaa gaagaaatct 
     7201 gctggacagc cggtcacctt cactgacatc ttcgggatgt tgataggaga gacgcttatt 
     7261 ccagggagaa tgaacattaa gctgagctca ttgaaaggga aaattaatga aggtcagagc 
     7321 cctcttcctc tcttcacctg tctgcacgtc aaacctgacg tctctgagct catgtttgca 
     7381 gattgggtgg agttcagccc ttatgaaatt ggcatggcca aatatggcac cttcatgtct 
     7441 cctgggctgt ttggcagcaa attcttcatg ggaagtgttg tgaaacaata tgaggaaaac 
     7501 cccttacatt tcctgatggg tgtgtgggga agtgcttttt ccatcctctt taaccgggtt 
     7561 ttgggtgtga aggagacgac cagcagtagc actatggagg aggagctgga gcaaattaaa 
     7621 ccagagcaca ttgtgggtga cgattctgct gataacgagg aggaaactca aagaggaggc 
     7681 acagagtctg cagatgcaga ggacgagcgc cagcgtcatg cccaggccag ctgggtacag 
     7741 cgcatgttga cctccatcat gggcgacacc actctgttca ccactcgtga gggccgcgca 
     7801 ggcaaagtgc acaacttcat gctgggactg aacctgaact caaccctgcc cttctctccc 
     7861 ttcagcggca tcacacacca gacctcgctg gaggaggaag tggacgctgt tactgatccg 
     7921 gatgagtttg agcgaatcta tgagcctctg gatgtgaaga gcaagaaaat ccatgtggtg 
     7981 gacagtggct tgacctttaa cctgccctac cctctcattc tgcggtgtca gagaggagtc 
     8041 gacctgatta tttcattcga cttctcagcc cgacccagtg actccagccc accgttcaaa 
     8101 gagttactgc tggctgaaaa atgggcccgt atgaataagc tgcccttccc gaagattgat 
     8161 tcgaaagtat tcgacagaga gggtctaaaa gagtgctatg tgttcaagcc tgctaaagga 
     8221 gacaagaact gccccaccat cattcatttt gtcttggcca acatcaactt ccggaatttt 
     8281 aaagcccctg gagttcctcg agattcagac aaggacatag agtttggtga ctttgacata 
     8341 tttgacgagc cagcctcccc ttactccact ttcaacttca aatacaacaa ccaggccttc 
     8401 aagagacttc atgacctcat ggagttcaat acgcttaaca acatcgaggt tatcaaagaa 
     8461 gccattaaag acagcattct gctgagacga gagaacccag cgcgatgttc ggtttccctt 
     8521 tcgctcagtg aaatcgaaaa caagaagttc ctgaaacgag acaacagcat tgcaaaacga 
     8581 cccaccggag ctggaggaat tcgtcctgga tctatggtgt ctaagggcga agagctgatt 
     8641 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag 
     8701 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcc 
     8761 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc 
     8821 agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct 
     8881 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc 
     8941 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac 
     9001 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag 
     9061 accctgtacc ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg 
     9121 ggcgggggcc acctgatctg caacttgaag accacataca gatccaagaa acccgctaag 
     9181 aacctcaaga tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc 
     9241 gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct 
     9301 agcaaactgg ggcacaaact gtaactcgag acccagcttt cttgtacaaa gtgggggatc 
     9361 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 
     9421 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 
     9481 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt 
     9541 gggaggtttt ttccaacttt a 
//
