LOCUS       #59_pAAAV_CMV-HB        8548 bp    DNA     circular SYN 25-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..2999
                     /label=Ribeye
     misc_feature    33..1712
                     /label=Unique N terminus
     CDS             complement(378..389)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(3018..3020,3021..3023,3024..3737)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    3759..4347
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(4230..4241)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      4847..5302
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5634..5738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5739..5807,5808..6599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6770..7358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        7809..8114
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    8130..8512
                     /label=HBA
ORIGIN
        1 ccccacctct gctgtattgc tttgctctgc caatgccggt tcccagcagg cacatcaaca
       61 ttggccgttc tcagagctgg gatgccgctg gctggtatga gggcccttgg gagaatgctg
      121 gccctccagg gaggagaagt tctctgactt atggccctgg agaaggggtc tggtgtgagc
      181 tggtaaacca ccgtgcccag gacactgagt cctgcctgtc ccgggaagct ttttataact
      241 cactggcatc aagaaagggc tccgtgcctg acttcacctt ctacgacagc aggcaggcag
      301 tgatgtctgg ccgtggctcc gtgctgccac aagactacta tggcgaccca tccaggggca
      361 ccagggtacc caaggagcct cccttctatc gggacccagg aaccagccgg ccggtgccca
      421 gctacggagt gctgggcagc agaataccat gggagcaggt acaaggccag ttacctgctc
      481 tgcaggatgc tggccacctg taccgggaat ctgggagtaa aactgtcctg catgggcagc
      541 ggacacactg cagggctcca tctcctggga ggtatggaag ggaacaacct gattctagat
      601 tagggataga ggtacccacc tattccccca actccagcca ggtctacaac gatatctgcg
      661 aaagacccgt tgactctacc catgccagac aggtggcccc aacatgcctg gttgtggacc
      721 ccagctcgac cgcccctact gagaacagca cgggggtggc cccaggttcc ctgaatagag
      781 gctacgggcc tacccgagaa agcatccatt caaagctggc ttatgagaat tatgaagccg
      841 acttgtcaac attccaagga cccgggggca agaggaccgt ctaccctgag tttttggccc
      901 tgctgagggc agagggtgtg gcagaggcta ccctggcagc cctgttacaa caaggctttg
      961 actccccagc tgtcttggcc accctagagg atgctgacat caagtctgtt gcccccaacc
     1021 tgggccaggc ccgtgtcctg agccgcctgg tcagcagctg caggacagag atgcagtttc
     1081 ggcgacagga ccggacaggc cctccacccc gccatcggtc cagtagcttc agccaccgaa
     1141 gtgagctgct gcccaatgac acggcctcgc tggggaccac agcactgcag tttcacccag
     1201 ctggaccttt gcagacaccc tctccccgag gtggagatct aggtcgccgc ccctccagtg
     1261 caccttccca gcacctcctg gagacagcag ctacttactc tgccccagtg gtggggtccc
     1321 agactcccca cttaccctcc aactctggat atagctctcc taccccttgt gccctaacag
     1381 cacgactggc ctcctcctac ccttcacaag ctggggtagc cttgactgct aacccaggcc
     1441 cctctgtccc ccttcactca agtcccagaa cagcctattc cacctcctac acggtgccca
     1501 tggaactgct taagagggaa cgcagtgtga ccgcctctcc gctgcccagc ccccatgcca
     1561 gcccccagct tctgcggaaa cctggagcag cccccgtgga gccggctgcc ctgccaccag
     1621 tcaggcaaag tctccacaca cctcatccgc cataccagaa ggtggcccgg cggacagggg
     1681 ctcccatcat cgtgtcaacc atgctaacac cagaaccaag tatccgcccc cagatcatga
     1741 acggccccct gcacccccgc cccctagtgg cactgctgga tggcagagac tgcactgtgg
     1801 aaatgcccat cctgaaggac ctggccactg tggccttctg tgatgcacag tccactcagg
     1861 aaatccatga gaaggtattg aatgaggctg tgggcgccat gatgtaccac accatcactc
     1921 tcacccggga agacctggag aagttcaagg ccctgagagt gattgttcga atcggtagtg
     1981 gctacgacaa tgtggacatc aaggctgctg gcgaactcgg aatcgccgtg tgcaacatcc
     2041 catctgctgc agtggaggag acagcagatt cgacagtctg ccatatcctc aatctgtatc
     2101 ggcggaacac atggctgtac caggccctac gagaaggcac gcgggtacaa agcgtggaac
     2161 agatccgtga ggtcgcctca ggagctgctc ggatccgagg ggaaacactg ggcctcattg
     2221 gctttggtcg cactgggcag gcagttgctg ttcgagccaa ggcctttgga ttcagcgtca
     2281 tattttatga cccctactta caggatggga tagagcggtc tctgggcgtg caaagggtct
     2341 acaccctgca ggacttgtta tatcagagcg actgtgtctc cttgcactgc aatctgaatg
     2401 aacacaacca ccacctcatc aatgacttca ccatcaagca gatgaggcaa ggagcattcc
     2461 ttgtgaatgc agcccgaggt ggcctggtgg atgagaaagc cttagctcag gccctcaaag
     2521 agggcaggat acgaggcgct gctctggatg tgcatgagtc tgagcccttc agctttgctc
     2581 agggcccatt gaaggatgct ccaaatctca tctgcacacc acacacagcc tggtacagcg
     2641 aacaagcatc actagagatg agggaagcag ctgccacgga gatccgccga gcaatcacag
     2701 gtcgcatccc agaaagctta cgaaactgtg tcaacaaaga attctttgtt acttcaactc
     2761 cttggtcagt cattgaccag caagcaattc atcctgagct caatggtgcc acatacaggt
     2821 atccaccagg cattgtaggt gtggctccag gagggcttcc tccagctatg gaagggatca
     2881 tccctggagg catccctgtg actcacaacc tccccactgt ggcacaccct tcccaagctc
     2941 cctccccaaa ccagcccaca aaacacgggg acaatcgaga gcaccccaac gagcaaatgg
     3001 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3061 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3121 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     3181 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     3241 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     3301 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     3361 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     3421 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     3481 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     3541 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     3601 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     3661 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     3721 acgagctgta caagtaaagc ggccgcaagc ttatcgataa tcaacctctg gattacaaaa
     3781 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3841 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3901 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3961 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4021 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4081 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4141 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     4201 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4261 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4321 ggatctccct ttgggccgcc tccccgcatc gataccgtcg actagagctc gctgatcagc
     4381 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4441 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4501 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4561 ggattgggaa gacaatagca ggcatgctgg ggagagatct aggaacccct agtgatggag
     4621 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg
     4681 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
     4741 gccaaccccc cccccccccc ccctgcagcc agctggcgta atagcgaaga ggcccgcacc
     4801 gatcgccctt cccaacagtt gcgtagcctg aatggcgaat ggcgcgacgc gccctgtagc
     4861 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4921 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4981 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5041 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5101 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5161 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5221 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5281 aaaatattaa cgtttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     5341 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5401 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5461 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5521 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5581 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5641 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5701 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5761 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5821 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5881 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5941 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6001 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6061 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6121 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6181 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6241 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6301 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6361 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6421 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6481 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6541 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6601 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6661 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6721 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6781 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6841 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6901 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6961 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7021 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7081 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7141 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7201 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7261 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7321 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7381 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7441 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7501 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7561 ctctccccgc gcgttggccg attcattaat gcagctgggc tgcagggggg gggggggggg
     7621 ggttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     7681 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agagagggag
     7741 tggccaactc catcactagg ggttcctaga tctaggatca cgcgttctag atcccatata
     7801 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     7861 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     7921 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     7981 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     8041 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     8101 tcgctattac catggtgatg cggttttggc ggccgggccc agcaccccaa ggcggccaac
     8161 gccaaaactc tcccccctcc tcttcctcaa tctcgctctc gctctttttt tttttcgcaa
     8221 aaggagggga gagggggtaa aaaaatgctg cactgtgcgg cgaagccggt gagtgagcgg
     8281 cgcggggcca atcagcgtgc gccgttccga aagttgcctt ttatggctcg agcggccggc
     8341 cgcggcggcg ccctataaaa cccagcggcg cgacgcgcca ccaccgccga gaccgcgtcc
     8401 gccccgcgag cacagagcct cgcctttgcc gatccgccgc ccgtccacac ccgccgccag
     8461 gtaagcccgg ccagccgacc ggggcaggcg gctcacggcc cggccgcagg cggccgccca
     8521 aagaattcgg cacgaggaaa ggcctcct
//
