LOCUS       pAAV_CMV-HBA_Rim        8055 bp    DNA     circular SYN 25-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     156..174
                     /label=Primer 7
     misc_feature    265..408
                     /label=ITR_AAV2
     enhancer        451..756
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    772..1154
                     /label=HBA
     primer_bind     903..920
                     /label=Primer 1
     misc_feature    1146..1148
                     /label=P2A Lang (Kim et al. 2011)
                     /note="P2A Lang (Kim et al. 2011)"
     CDS             1166..4435
                     /codon_start=1
                     /gene="Rimbp2"
                     /gene_synonym="A930033C01Rik; mKIAA0318"
                     /product="RIMS-binding protein 2"
                     /label=Rimbp2
                     /note="RIM-BP2"
                     /note="/dnas_title=RIMS-binding protein 2"
                     /db_xref="GI:124487059"
                     /db_xref="CCDS:CCDS39288.1"
                     /db_xref="GeneID:231760"
                     /db_xref="MGI:2443235"
                     /protein_id="NP_001074857.1"
                     /translation="MREAAERRQQLELEHEQALAFLNAKQQEIQLLQQAQVEAKKEHEG
                     AVQLLENTLDCMQSKVRELEEKCRVQSEQFNLLSRDLEKFRQHTGSIDLLGSSSVALLD
                     VPLAPGKPFPQYMNGLATSIHKGHEGPTGHYSVIGDYIPLSGDKLESPCVKPSFLLRSS
                     SPRCRFESEMDNDRNSNNSKQSSSGKVHLCVARYSYNPFDGPNENPEAELPLTAGKYLY
                     VYGDMDEDGFYEGELLDGQRGLVPSNFVDFIQDNESRLAGTLGSEQDQNFLNHSGISLE
                     RDSILHLHSPTQVDSGITDNGGGTLDVNIDDIGEDTVPYPRKITLIKQLAKSVIVGWEP
                     PAVPPGWGTVSSYNVLVDKETRMSLALGRRTKALIEKLNTAACTYRISVQCVTSRGNSD
                     ELQCTLLVGKDVVVAPSQLRVDNITQISAQLSWLPTNSNYSHIIFLNEEELDIVKAARY
                     KYQFFNLRPNMAYKVKVLAQPHQMPWQLPLEQREKKEACVEFSTLPAGPPAPPQDVTVH
                     AGATAASVQVSWKPPALTPTGLSNGANVTGYGVYAKGQRVAEVIAPTADGTAVELIRLR
                     SLEAKAVSVRTLSVQGESMDSALAAIPPDLLVPPAPHPRTAPPPKPLASDMDTKDQHLG
                     PHVKVDESWEQSRSPGPAHGHMLEPPDMHSAGPGRRSPSPSRILPQPQGAPVSTTVAKA
                     MAREAAQRVAESNRLEKRSLFLEQSSAGQYTNSDEEDGYASPEVKRRGTSVDDFLKGSE
                     LGKQPHCCHGDEYHTESSRGSDLSDIMEEDEEELYSEMQLEDGGRRRPSGTSHNALKIL
                     GNSTLMGRADRMEHVSRRYSHSGGGSHRHRPAMAPSIDEYTGRDHLSPDFYDESETDPG
                     AEELPARIFVALFDYDPLTMSPNPDAAEEELPFKEGQIIKVYGDKDADGFYRGETCARL
                     GLIPCNMVSEIHADDEEMMDQLLRQGFLPLNTPVEKIERSRRSGRGHSVPTRRMVALYD
                     YDPRESSPNVDVEAELPFCTGDIITVFGEIDEDGFYYGELNGQKGLVPSNFLEEVPDDV
                     EVHLSDAPPHYSHDPPMRSKAKRKKSVHFTPEQKLISEEDL"
     misc_feature    1166..1267
                     /label=exon4
                     /note="exon4"
     misc_feature    complement(1224..1243)
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="RBP2-59rev"
     misc_feature    1225..1267
                     /label=start sequence deleted in KO
                     /note="start sequence deleted in KO"
     misc_feature    1268..1318
                     /label=exon5
                     /note="exon5"
     conflict        1317..1337
                     /label=sequence not in cDNA sequence
                     /note="sequence not in cDNA sequence"
     misc_feature    1319..1544
     misc_feature    1340..1544
                     /label=exon6
                     /note="exon6"
     misc_feature    complement(1355..1374)
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="RBP2-189rev"
     primer_bind     1421..1440
                     /label=Primer 2
     CDS             1742..1744
                     /label=additionalCys
     misc_feature    1745..1915
                     /label=SH3.1
                     /note="SH3.1"
     primer_bind     1897..1915
                     /label=Primer 3
     V_region        2636..2773
                     /label=Schoch SH3.2
                     /note="Schoch SH3.2"
     primer_bind     2916..2935
                     /label=Primer 4
     primer_bind     3410..3429
                     /label=Primer 5
     misc_feature    3666..3684
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="RT fw primer"
                     /note="/current=0"
     misc_feature    complement(3714..3731)
                     /label=PCR_primer
                     /label=nonstandard type:  PCR_primer
                     /note="RT reverse primer"
                     /note="/current=0"
     misc_feature    3740..3742
                     /gene="Rimbp2"
                     /gene_synonym="A930033C01Rik; mKIAA0318"
                     /note="Phosphoserine; propagated fromUniProtKB/Swiss-Prot 
                     (Q80U40.2); phosphorylation site"
                     /experiment="experimental evidence, no additional 
                     detailsrecorded"
     V_region        3797..3985
                     /label=SH3.2
                     /note="SH3.2"
     primer_bind     4092..4111
                     /label=Primer 6
     V_region        4115..4291
                     /label=SH3.3
                     /note="SH3.3"
     misc_signal     4403..4432
                     /label=Myc Tag
                     /note="Myc Tag"
     misc_feature    4456..5044
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(4927..4938)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    5084..5289
                     /label=bGH poly(A) signal
     misc_feature    5299..5441
                     /label=ITR_AAV2
     rep_origin      5544..5999
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6331..6435
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6436..6504,6505..7296)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7467..8055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 gctgcagggg gggggggggg ggggttggcc actccctctc tgcgcgctcg ctcgctcact
      301 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
      361 gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttccta gatctaggat
      421 cacgcgttct agatcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      481 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      541 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      601 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      661 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      721 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcggccgggc
      781 ccagcacccc aaggcggcca acgccaaaac tctcccccct cctcttcctc aatctcgctc
      841 tcgctctttt tttttttcgc aaaaggaggg gagagggggt aaaaaaatgc tgcactgtgc
      901 ggcgaagccg gtgagtgagc ggcgcggggc caatcagcgt gcgccgttcc gaaagttgcc
      961 ttttatggct cgagcggccg gccgcggcgg cgccctataa aacccagcgg cgcgacgcgc
     1021 caccaccgcc gagaccgcgt ccgccccgcg agcacagagc ctcgcctttg ccgatccgcc
     1081 gcccgtccac acccgccgcc aggtaagccc ggccagccga ccggggcagg cggctcacgg
     1141 cccggccgca ggcggccgcg ccaccatgcg agaggctgct gagcggcggc aacagctgga
     1201 gttggagcat gagcaagccc tggccttcct caatgccaag cagcaggaga tccagctact
     1261 gcagcaggct caggttgaag ctaagaaaga gcatgaaggt gctgtgcagc tgttagagaa
     1321 caccctggac tgcatgcagt ccaaggtccg agagctggaa gagaaatgcc gtgtgcagag
     1381 tgagcagttt aacttgttgt cccgggacct ggaaaagttc cggcaacaca ctgggagtat
     1441 tgacctgctg ggcagcagct cagtggccct gctggatgtt cccttggccc ctggcaagcc
     1501 tttccctcag tacatgaatg gactagccac ctccatccac aaaggtcacg agggccccac
     1561 tggacactac tctgtgattg gtgactatat tccgctgtct ggggacaagc tggagtctcc
     1621 gtgtgtgaag ccctccttcc tcttgcgatc cagcagccca agatgcagat ttgagtccga
     1681 gatggacaat gaccggaact ctaacaactc caagcaaagc agctcgggga aggtgcacct
     1741 gtgtgtggcc cgctacagtt acaacccctt cgatgggccc aatgagaacc cagaagctga
     1801 gctgcctctc acggcgggaa agtacctcta tgtctatggg gacatggatg aagatgggtt
     1861 ctatgaagga gagcttctgg atgggcaaag gggcttggtg ccctccaact ttgtggattt
     1921 tatccaggac aatgagtcgc ggctggctgg tactctgggg agtgagcagg accagaactt
     1981 tctcaaccac tctggcatca gtctggagcg cgacagcatc cttcaccttc actccccaac
     2041 tcaagtggac tcggggatca ccgacaatgg tggggggacc ctggacgtga acatcgacga
     2101 catcggagaa gacaccgtgc cttaccctag gaaaatcacc cttatcaaac agcttgccaa
     2161 aagtgtcatc gtgggctggg agccccctgc tgtgcctccc ggctggggca ccgtgagcag
     2221 ttataatgtg ctggtggaca aagagacacg catgagcctt gccctgggta ggagaactaa
     2281 ggcgctgatc gagaaactta acacagctgc ctgcacctac cgcatctccg tgcagtgcgt
     2341 cacgagccgg ggaaactcag acgagctgca gtgcactctg ctggtgggca aggatgtggt
     2401 ggtggcaccg tcccagctgc gtgtggacaa catcacacag atctctgccc agctctcgtg
     2461 gctgccgacc aacagtaact acagccatat catcttcctc aacgaagaag aactggacat
     2521 cgtgaaggca gccaggtaca agtaccaatt cttcaacctc aggcctaata tggcctacaa
     2581 ggtgaaggtc ttggcccagc cacaccagat gccctggcag ctgccgctgg agcagagaga
     2641 aaagaaggag gcctgtgtgg agttctccac gctgcctgca ggacctccag ccccaccaca
     2701 agatgtcact gtccacgctg gggccacagc cgcctctgtt caggtctcct ggaagccccc
     2761 tgcactgact cccactgggt tgtccaatgg agcaaatgtc acaggatacg gcgtgtacgc
     2821 caaagggcag agggtggctg aggtcatcgc ccccacggca gatggcacgg cagtggagct
     2881 gatccggctg cgaagcctag aggccaaggc cgtgagtgtg cgtaccctgt ctgtacaggg
     2941 agagtccatg gattctgccc tcgctgccat cccccctgac ctcctggtgc ctccagcccc
     3001 ccacccgagg actgctcccc caccaaagcc attagcaagt gacatggata ccaaagacca
     3061 acacctgggg ccccacgtca aagtggatga gtcctgggag cagagccggt caccgggccc
     3121 tgcacatggc cacatgttgg aaccacctga catgcacagc gctggcccag gcagaaggtc
     3181 accctcgccc agccggatcc ttcctcagcc acaaggagcc cccgtgtcta ccactgtcgc
     3241 caaggccatg gcccgtgaag ctgcacagag ggtggctgag agcaacaggt tagagaaaag
     3301 gagcctcttt ctagagcaaa gcagtgcggg gcaatatacc aactcggacg aggaggacgg
     3361 ctacgcctcc cccgaggtca agaggagagg cacctcagtg gatgacttcc tcaaagggtc
     3421 agagctgggc aagcagcccc actgttgcca tggagatgag taccacacag agagcagccg
     3481 ggggtcagac ctgtcggaca tcatggagga ggatgaggag gagctatact cagagatgca
     3541 gctggaggat gggggccgcc gtcggcccag cggtacctct cacaacgccc tcaagatttt
     3601 aggaaactcc acgttgatgg gacgagcaga ccggatggaa cacgtgagcc gaaggtattc
     3661 acacagtggc ggagggtctc ataggcaccg cccagcgatg gctccatcca ttgatgaata
     3721 caccgggcga gaccatcttt ctccagactt ctatgatgag tccgaaactg accctggtgc
     3781 tgaggagctc ccagcccgta tctttgtggc tctgtttgac tatgacccac tgaccatgtc
     3841 cccaaaccca gacgctgctg aagaggagct tcccttcaaa gaaggacaga tcatcaaggt
     3901 atatggagac aaagacgcag atggcttcta ccgtggggag acctgtgcca ggctcggcct
     3961 cattccctgt aacatggtct ctgagatcca tgcggatgat gaggagatga tggatcagct
     4021 gctgaggcaa ggcttcctcc ctctgaacac gcctgtggag aaaatagaga gaagtagaag
     4081 aagcggccgg ggtcactctg tacccacacg aagaatggtg gctctctacg actatgatcc
     4141 tagggaaagc tctcctaacg tggatgttga ggctgaactt ccattttgca caggagacat
     4201 tattactgtt tttggtgaaa tcgatgaaga tggattttat tatggagagc tgaatgggca
     4261 aaaaggcctc gtgccttcca atttcctgga agaagtgcct gatgatgtgg aggtccacct
     4321 ttctgatgct ccgccccact actcccacga cccgcccatg cgctccaagg ccaaaaggaa
     4381 gaagagtgtt catttcacac ccgaacaaaa acttatttct gaagaagatc tgtaagcggc
     4441 cgcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4501 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4561 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4621 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4681 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4741 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4801 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4861 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgcc cttctgctac
     4921 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4981 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5041 ccgcatcgat accgtcgact agagctcgct gatcagcctc gactgtgcct tctagttgcc
     5101 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     5161 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     5221 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     5281 atgctgggga gagatctagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     5341 tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc
     5401 ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aacccccccc cccccccccc
     5461 tgcagccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     5521 tagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5581 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5641 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5701 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5761 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5821 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5881 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5941 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc
     6001 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     6061 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     6121 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     6181 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     6241 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     6301 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6361 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6421 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6481 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6541 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6601 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6661 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6721 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6781 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6841 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6901 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6961 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7021 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7081 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7141 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7201 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7261 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7321 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7381 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7441 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7501 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7561 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     7621 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7681 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7741 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7801 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     7861 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7921 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7981 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8041 cggagcctat ggaaa
//
