LOCUS       pAC005-pesumo-nu        7223 bp DNA     circular SYN 05-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 29, 2025 from SnapGene Viewer 7.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(756..1838)
                     /label=lacI
     promoter        complement(1839..1916)
                     /label=lacI promoter
     promoter        2225..2243
                     /label=T7 promoter
     protein_bind    2244..2268
                     /label=lac operator
     RBS             2283..2305
                     /label=RBS
     CDS             2312..2635
                     /codon_start=1
                     /label=Translation 2312-2635
                     /translation="MGHHHHHHGSLQDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEI
                     FFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHRE
                     QIGG"
     CDS             2318..2335
                     /label=6xHis
     CDS             2348..2635
                     /label=SUMO
     misc_feature    2636..4123
                     /label=NusA
     CDS             2636..4123
                     /codon_start=1
                     /label=Translation 2636-4123
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDV
                     RVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDR
                     ITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVIL
                     REDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIE
                     IKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPA
                     QFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMT
                     VDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLD
                     EPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLED
                     LAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     CDS             4175..4192
                     /label=6xHis
     terminator      4259..4306
                     /label=T7 terminator
     rep_origin      4343..4798
                     /label=f1 ori
     promoter        4825..4929
                     /label=AmpR promoter
     CDS             4930..4998
                     /label=AmpR
     CDS             4999..5790
                     /label=AmpR
     rep_origin      5961..6549
                     /label=ori
     misc_feature    6735..6877
                     /label=bom
ORIGIN
        1 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
       61 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
      121 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
      181 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
      241 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
      301 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
      361 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
      421 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
      481 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
      541 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
      601 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
      661 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
      721 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      781 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      841 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
      901 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
      961 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     1021 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     1081 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     1141 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     1201 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     1261 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     1321 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     1381 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     1441 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     1501 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     1561 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     1621 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     1681 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     1741 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     1801 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     1861 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     1921 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     1981 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     2041 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     2101 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     2161 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     2221 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     2281 attttgttta actttaagaa ggagatatac catgggtcat caccatcatc atcacgggtc
     2341 cctgcaggac tcagaagtca atcaagaagc taagccagag gtcaagccag aagtcaagcc
     2401 tgagactcac atcaatttaa aggtgtccga tggatcttca gagatcttct tcaagatcaa
     2461 aaagaccact cctttaagaa ggctgatgga agcgttcgct aaaagacagg gtaaggaaat
     2521 ggactcctta agattcttgt acgacggtat tagaattcaa gctgatcagg cccctgaaga
     2581 tttggacatg gaggataacg atattattga ggctcaccgc gaacagattg gaggtatgaa
     2641 caaagaaatt ttggctgtag ttgaagccgt atccaatgaa aaggcgctac ctcgcgagaa
     2701 gattttcgaa gcattggaaa gcgcgctggc gacagcaaca aagaaaaaat atgaacaaga
     2761 gatcgacgtc cgcgtacaga tcgatcgcaa aagcggtgat tttgacactt tccgtcgctg
     2821 gttagttgtt gatgaagtca cccagccgac caaggaaatc acccttgaag ccgcacgtta
     2881 tgaagatgaa agcctgaacc tgggcgatta cgttgaagat cagattgagt ctgttacctt
     2941 tgaccgtatc actacccaga cggcaaaaca ggttatcgtg cagaaagtgc gtgaagccga
     3001 acgtgcgatg gtggttgatc agttccgtga acacgaaggt gaaatcatca ccggcgtggt
     3061 gaaaaaagta aaccgcgaca acatctctct ggatctgggc aacaacgctg aagccgtgat
     3121 cctgcgcgaa gatatgctgc cgcgtgaaaa cttccgccct ggcgaccgcg ttcgtggcgt
     3181 gctctattcc gttcgcccgg aagcgcgtgg cgcgcaactg ttcgtcactc gttccaagcc
     3241 ggaaatgctg atcgaactgt tccgtattga agtgccagaa atcggcgaag aagtgattga
     3301 aattaaagca gcggctcgcg atccgggttc tcgtgcgaaa atcgcggtga aaaccaacga
     3361 taaacgtatc gatccggtag gtgcttgcgt aggtatgcgt ggcgcgcgtg ttcaggcggt
     3421 gtctactgaa ctgggtggcg agcgtatcga tatcgtcctg tgggatgata acccggcgca
     3481 gttcgtgatt aacgcaatgg caccggcaga cgttgcttct atcgtggtgg atgaagataa
     3541 acacaccatg gatatcgccg ttgaagccgg taacctggcg caggcgattg gccgtaacgg
     3601 tcagaacgtg cgtctggctt cgcagctgag cggttgggaa ctcaacgtga tgaccgttga
     3661 cgacctgcag gctaagcatc aggcggaagc gcacgcagcg atcgacacct tcaccaaata
     3721 tctcgacatc gacgaagact tcgcgactgt tctggtagaa gaaggcttct cgacgctgga
     3781 agaattggcc tatgtgccga tgaaagagct gttggaaatc gaaggccttg atgagccgac
     3841 cgttgaagca ctgcgcgagc gtgctaaaaa tgcactggcc accattgcac aggcccagga
     3901 agaaagcctc ggtgataaca aaccggctga cgatctgctg aaccttgaag gggtagatcg
     3961 tgatttggca ttcaaactgg ccgcccgtgg cgtttgtacg ctggaagatc tcgccgaaca
     4021 gggcattgat gatctggctg atatcgaagg gttgaccgac gaaaaagccg gagcactgat
     4081 tatggctgcc cgtaatattt gctggttcgg tgacgaagcg taactagagg atccgaattc
     4141 gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
     4201 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     4261 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     4321 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4381 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4441 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4501 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4561 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4621 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4681 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4741 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttca
     4801 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4861 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4921 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4981 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5041 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5101 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5161 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5221 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5281 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5341 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5401 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5461 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5521 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5581 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5641 cgtggttctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5701 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5761 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5821 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5881 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5941 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6001 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6061 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6121 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6181 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6241 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6301 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6361 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6421 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6481 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6541 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6601 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6661 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6721 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     6781 cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     6841 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     6901 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     6961 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     7021 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     7081 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     7141 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     7201 catgggggta atgataccga tga
//
