LOCUS       pLlacO1-DP6             7499 bp    DNA     circular SYN 24-JUN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Nicholas Southern
  TITLE     Direct Submission
  JOURNAL   Exported Jun 24, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7499
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="recombinant plasmid"
     modified_base   complement(1..16)
                     /label=USER junction
                     /note="USER junction"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(17..114)
                     /note="cat promoter"
                     /note="from pACYCDuet-1"
                     /note="color: #ffffff; direction: LEFT"
     CDS             115..774
                     /label=cat (CmR)
                     /note="cat (CmR)"
                     /note="from pACYCDuet-1"
                     /note="color: #993366; direction: RIGHT"
     modified_base   699
                     /label=mutation
                     /note="mutation"
                     /note="annotated as a G in pACYCDuet cat markerannotation,
                     here it is an A, but this mutation is silent from codon GTC
                     (Val, 25% codon usage) to GTT (Val, 21% usage), so it 
                     should not be of functional relevance"
                     /note="color: #a6acb3"
     modified_base   complement(775..787)
                     /label=USER junction
                     /note="USER junction"
                     /note="color: #a6acb3; direction: LEFT"
     terminator      788..823
                     /note="P14/tonB bidirectional terminator"
                     /note="termination of cat transcript is slightly weakerthan
                     in opposite direction"
                     /note="color: #ffffff"
     modified_base   complement(831..847)
                     /label=USER junction
                     /note="USER junction"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(848..1474)
                     /label=pmCDA1 (opt)
                     /note="pmCDA1 (opt)"
                     /note="color: #993366; direction: LEFT"
     RBS             complement(1475..1495)
                     /label=dnaE RBS
                     /note="dnaE RBS"
                     /note="color: #a6acb3; direction: LEFT"
     modified_base   complement(1481..1499)
                     /label=USER Junction
                     /note="USER Junction"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(1504..1758)
                     /label=PBS2 UGI
                     /note="PBS2 UGI"
                     /note="color: #993366; direction: LEFT"
     RBS             complement(1759..1779)
                     /label=Native UGI RBS
                     /note="Native UGI RBS"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(1787..2317)
                     /label=emrR (wt)
                     /note="emrR (wt)"
                     /note="color: #993366; direction: LEFT"
     RBS             complement(2318..2337)
                     /label=native emrR RBS
                     /note="native emrR RBS"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(2346..2891)
                     /label=seqA (wt)
                     /note="seqA (wt)"
                     /note="color: #993366; direction: LEFT"
     RBS             complement(2892..2911)
                     /label=seqA Native RBS
                     /note="seqA Native RBS"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(2920..3756)
                     /label=dam (wt)
                     /note="dam (wt)"
                     /note="color: #993366; direction: LEFT"
     RBS             complement(3757..3775)
                     /label=Modified mutS RBS
                     /note="Modified mutS RBS"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(3785..4516)
                     /codon_start=1
                     /label=dnaQ926
                     /note="/dnas_title=dnaQ926"
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MSTAITRQIVLATATTGMNQIGAHYEGHKIIEIGAVEVVNRRLTG
                     NNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAFDI
                     GFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRTLHG
                     ALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFATDEEI
                     AAHEARLDLVQKKGGSCLWRA"
     conflict        4475..4477
                     /label=E14A
                     /note="E14A"
                     /note="color: #a6acb3"
     conflict        4481..4483
                     /label=D12A
                     /note="D12A"
                     /note="color: #a6acb3"
     RBS             4523..4536
                     /label=B0031
                     /note="color: #a6acb3"
     promoter        complement(4543..4617)
                     /label=pLlacO-1
                     /label=pLlac
                     /note="Modified phage lambda PL promoter with LacI operator
                     sites (Lutz and Bujard, 1997).
                     Placed under control of Mutagenesis. (Broedel et al, 2020) 
                     "
                     /note="color: #3366ff; direction: LEFT"
     protein_bind    4575..4591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     protein_bind    4597..4613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     modified_base   complement(4618..4632)
                     /label=USER junction
                     /note="USER junction"
                     /note="color: #a6acb3; direction: LEFT"
     terminator      4633..5057
                     /note="rrnB1 transcriptional terminator"
                     /note="color: #ffffff"
     misc_feature    complement(5065..5077)
                     /label=USER linker
                     /note="USER linker"
                     /note="color: #a6acb3; direction: LEFT"
     enhancer        5078..5125
                     /note="Protected by pspF"
                     /note="color: #a6acb3"
     protein_bind    5078..5092
                     /note="UAS II"
                     /note="color: #31849b"
     protein_bind    5080..5089
                     /label=pspF
                     /note="/%_Identity=100"
                     /note="/Motif=TGAAATTCGC"
                     /note="/annotation_group=TGAAATTCGC: 6,220 -> 6,229"
                     /note="/modified_by=tinawang"
                     /note="color: #31849b"
     RBS             5085..5094
                     /label=pspF RBS
                     /note="pspF RBS"
                     /note="color: #a6acb3"
     prim_transcript 5094
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5096..5114
                     /note="UAS I"
                     /note="color: #31849b"
     promoter        complement(5098..5125)
                     /note="pspF P2"
                     /note="color: #ffffff; direction: LEFT"
     -10_signal      complement(5098..5103)
                     /label=-10
                     /note="-10"
                     /note="color: #a6acb3; direction: LEFT"
     protein_bind    5100..5109
                     /label=pspF
                     /note="/%_Identity=100"
                     /note="/Motif=TGTAATTCGC"
                     /note="/annotation_group=TGTAATTCGC: 6,240 -> 6,249"
                     /note="/modified_by=tinawang"
                     /note="color: #31849b"
     prim_transcript 5110
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        complement(5116..5144)
                     /note="pspF P1"
                     /note="color: #ffffff; direction: LEFT"
     -10_signal      complement(5116..5121)
                     /label=-10
                     /note="-10"
                     /note="color: #a6acb3; direction: LEFT"
     -35_signal      complement(5120..5125)
                     /label=-35
                     /note="-35"
                     /note="color: #a6acb3; direction: LEFT"
     -35_signal      complement(5139..5144)
                     /label=-35
                     /note="-35"
                     /note="color: #a6acb3; direction: LEFT"
     protein_bind    5142..5174
                     /note="High Affinity IHF Site"
                     /note="color: #31849b"
     promoter        5176..5192
                     /note="Sigma54 Core Promoter"
                     /note="color: #ffffff"
     -35_signal      5176..5183
                     /label=-35
                     /note="/created_by=tinawang"
                     /note="color: #a6acb3"
     -35_signal      5178..5179
                     /label=-24
                     /note="-24"
                     /note="color: #a6acb3"
     -10_signal      5188..5192
                     /label=-10
                     /note="/created_by=tinawang"
                     /note="color: #a6acb3"
     -10_signal      5190..5191
                     /label=-12
                     /note="-12"
                     /note="color: #a6acb3"
     prim_transcript 5203
                     /label=Transcription Start Site
                     /note="Transcription Start Site"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5204..5222
                     /note="tetR binding site"
                     /note="color: #31849b"
     prim_transcript 5224
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        complement(5231..5258)
                     /note="pspF P3"
                     /note="color: #ffffff; direction: LEFT"
     -10_signal      complement(5231..5236)
                     /label=-10
                     /note="-10"
                     /note="color: #a6acb3; direction: LEFT"
     -35_signal      complement(5253..5258)
                     /label=-35
                     /note="-35"
                     /note="color: #a6acb3; direction: LEFT"
     modified_base   5263..5277
                     /label=USER junction
                     /note="USER junction"
                     /note="color: #a6acb3"
     RBS             5278..5291
                     /label=sd8 RBS (from Ringquist and Gold  Mol. Micro.1992)
                     /note="sd8 RBS (from Ringquist and Gold  Mol. Micro.1992)"
                     /note="color: #a6acb3"
     CDS             5292..6566
                     /codon_start=1
                     /label=III
                     /note="/dnas_title=III"
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     misc_feature    6434..6479
                     /label=modified to remove internal promoter
                     /note="modified to remove internal promoter"
                     /note="color: #a6acb3"
     modified_base   complement(6567..6581)
                     /label=USER junction
                     /note="USER junction"
                     /note="color: #a6acb3; direction: LEFT"
     misc_difference complement(6582..6588)
                     /label=cloning scar
                     /note="cloning scar"
                     /note="color: #a6acb3; direction: LEFT"
     terminator      complement(6589..6633)
                     /note="rrnB1 transcriptional terminator"
                     /note="color: #ffffff; direction: LEFT"
     modified_base   complement(6634..6648)
                     /label=USER junction
                     /note="USER junction"
                     /note="color: #a6acb3; direction: LEFT"
     modified_base   complement(6694..6711)
                     /label=User Junction
                     /note="User Junction"
                     /note="color: #a6acb3; direction: LEFT"
     rep_origin      6723..7461
                     /label=cloDF13
                     /note="/dnas_title=cloDF13"
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
ORIGIN
        1 acggcctatt tggcttggca cgtaagaggt tccaactttc accataatga aataagatca
       61 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
      121 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
      181 gaggcatttc agtcagttgc taaatgtacc tataaccaga ccgttcagct ggatattacg
      241 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
      301 cttgcccgcc tgatgaatgc tcatccggag ttccgtatgg caatgaaaga cggtgagctg
      361 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
      421 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
      481 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
      541 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
      601 atggacaact tcttcgcccc cgttttcact atgggcaaat attatacgca aggcgacaag
      661 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
      721 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcca
      781 tgggcatgta gtcaaaagcc tccggtcgga ggcttttgac ttggctgagg agtgccgtta
      841 attaagttta aaccgccgga gatttggtgg tgtgcaggat tttaacctgg atcatgatag
      901 acagttcaga acgacgtttt tccgcacgtt tcagggtttt ttccagccaa cggttttcgt
      961 tcagctggtt gtgagaagac tggatgaaga ttttacggca gcactggtag tgttcagaaa
     1021 ccataacgtt cagaccaaca ccgttgtcac gcaggttcca cagaccgatc tggttacgcg
     1081 cgtttttttc gtagtacagt ttgcacgccc agattttcag ggtgtgaccg ttaccacgca
     1141 gttcctggtt gtaccattcc aggatttttt ccgcgcagtc cgcgcacgga gaccaagaag
     1201 agtaccagtt gatggtgaac tgacccgggt tgtcacgcag gtattcttca actttacgga
     1261 tagagaagat ttccgcgtgg ataccacgtt cggtaccaga ctgcggtttg ttaaccgcgt
     1321 aaccccagaa gcacgcacga cgttcaccac gacgtttcag ttcgaacaga acgtagcaac
     1381 ggtgagaaac agattttttg ttgttgaaga actgtttttt gaaggtgtag atgtccagtt
     1441 tttcgtggat acgaacgtat tccgcgtcgg tcatcttcag attccggttt acttaatctc
     1501 gacttataac attttaattt tattttctcc attactgtct tgaattacta aagcccaagg
     1561 tttatattct ggagcatctg aagttaatag cattacattt tcatctgtac tttcatcata
     1621 agcagtatga actaaaatat cactttctgg tttattccca attacttcct ctacttcttc
     1681 tggtaacatt agaattgatt cttgaatcac tagttgtttt cctgtttctt tttcaatgat
     1741 gtcagataaa tttgtcatgt tgaaattcct cctaattttt gtattattag ctcatcgctt
     1801 cgagaaccac accgtcttgt tccatctggt cgagacggga gagcaatttg cgggtgattt
     1861 gctcgagctg atctttttct gttgtgctga gcgcggacca gagttgatgc aggcagttat
     1921 gctgcggtgg taaaacctcg cgcaaaaact cgtgaccttt ttccgttaat tgcagatgca
     1981 ggcagcggcg atcgttatcg ctttcacgac gttcgatcca accgcgtttt tccagttcat
     2041 cggcaatacg cgtcgcgttg gtacgggatg atccaagagc acaacttaat tcagaaggct
     2101 gaatactgtg gttttcctga gactccagcg taatcaacgc cataaacaac gtctcgttaa
     2161 ttccctgagc cttcagcatt ttattgcggt tctccagcag cttgctttgc atgtgcatgc
     2221 aaagacgagt cagaaggatc tcctgataag gaaaatcttc gtggcggctg gcgcgaaatt
     2281 ttagcatttg ttcaatgggc gtaaacgaac tatccatttg ggtatgacct cattaatttc
     2341 agccgttaga tagttccgca aaccttctca atcaattccg ccgggaattg catcgactgc
     2401 atgatgtgtt cgatcatgct gcatttacgg ccggtgttgg tgttggtgat cacccaatac
     2461 ggcgtgcctg gcacatgttt cggcttggtc tgattaccat ttttcagcag cgtttgttca
     2521 tctgccgcaa agtaaacgcg tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc
     2581 tgggcgtcaa gagaatatag tgtagacaac agcagcataa agcgattgac cgctcgcttt
     2641 tgctctgcgt attcatccga aagcagaagt tcacgcattg cgcgaacctt gtctttaatc
     2701 gttttgaccg gcttcgcttc gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc
     2761 ggagcagcag gctgtgatgc ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg
     2821 ctctcgccga tatgcttagt gtggctggca atatagctgt agagttcatc atcaacttca
     2881 atcgttttca tcttaatcca gtgcagtgtc ttagctgaat tattttttcg cgggtgaaac
     2941 gactcctggt ttgtacaaag ccagcagttc gtccaccttt ttacgtgtgc cgccgttgct
     3001 gcttatactg cgtcgaactt tgacgacatg caattttgcg cgctgatacc actcacgcgt
     3061 taacatcgta tcgtgattgg agatcagcac tggaatatgg cgctcaacca gaccttcggc
     3121 gatctccgcc agatgcgctt gttgttcaag cgtaaaactg tttgtgtgat acgccgtaaa
     3181 gttggcggtc gcagacagcg gtgcataagg cggatcgcaa tagacgacgg atgcatcatc
     3241 tgcgcgcgcc atgctatcgg cgtaagactc acaatagaaa aaggcattct gcgctttttc
     3301 agcgaagtga tacaactctg cttccgggaa atagggtttt ttgtagcggc cgaacggcac
     3361 gttaaactca ccgcgcagat tgtaacgaca caggccgttg taaccgtagc ggttcaaata
     3421 taaaaacagt accgcccgac ggaacggatc ctggcttttg ttgaactctt cgcggaactg
     3481 atagtaaacc tcggcgcaat ttgtttcggg aacaaacagc tcgcgtgcgg cctgtacgta
     3541 ctcatcagta cgcatcttca caatgttata gagactgatc aggtcgctat tgatatcggc
     3601 aaggatataa cgagaaaagt cggtgttgag aaacaccgac ccggcaccta caaaaggctc
     3661 aaccagacat tcgcccttgg gcaaatgccg tttaatatca tcaagcaggg gatacttgcc
     3721 ccctgcccac ttcaaaaaag cgcgattttt cttcatgggt tatgtccggt ttactgatat
     3781 taaattatgc tcgccagagg caacttccgc ctttcttctg caccagatcg agacgggctt
     3841 catgagctgc aatctcttca tctgtcgcaa aaacaacgcg taacttactt gcctgacgta
     3901 caatgcgctg aattgttgct tcaccttgtt gctgttgtgt ctctccttcc atcgcaaaag
     3961 ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc cgcaaggatc tgggcatcga
     4021 gtaatgcccc gtgcagcgtt cgtttactgt tatctatttc gtagcgagca cataacgcat
     4081 cgaggctgtt gcgcttaccg ggaaacattt tcctcgccac cgcaaggcta tcggtgacct
     4141 tacagaaagt attggtcttc ggaatatcgc gcttaagcaa cgaaaactcg tagtccataa
     4201 agccgatatc gaacgctgcg ttatggatca ccaactccgc gccgcgaata tagtccatga
     4261 actcatcggc tacttcggca aacgtgggct tatcgagcaa aaattcatcg gcaataccat
     4321 gtacgccaaa ggcttccgga tccaccagcc gatcgggttt gagataaaca tggaagttat
     4381 tgcccgtcag gcgacggttc accacttcaa cggcaccaat ctcaatgatc ttgtggcctt
     4441 catagtgcgc accaatctgg ttcataccgg tggttgcggt agcgagaacg atctggcgtg
     4501 taattgcagt gctcatctag taggtttcct gtgtgagtct tcggtcagtg cgtcctgctg
     4561 atgtgctcag tatcttgtta tccgctcaca atgtcaatgt tatccgctca catttatagg
     4621 gtccctaagt ctggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
     4681 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
     4741 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     4801 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     4861 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     4921 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     4981 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     5041 tgcgtttcta caaactcgct gaggacattg gctccgtgat gaaattcgcc acttgttagt
     5101 gtaattcgct aactcatcct ggcatgttgc tgttgattct tcaatcagat ctttataaat
     5161 caaaaagata aaaaattggc acgcaaattg tattaacagt tcatccctat cagtgataga
     5221 gagcaggaca atcctgaacg cagaaatcaa gaggacaaca ttacctgcag gtgcagtaaa
     5281 ggaaaaaaaa aatgaaaaaa ttattattcg caattccttt agttgttcct ttctattctc
     5341 actccgctga aactgttgaa agttgtttag caaaacccca tacagaaaat tcatttacta
     5401 acgtctggaa agacgacaaa actttagatc gttacgctaa ctatgagggc tgtctgtgga
     5461 atgctacagg cgttgtagtt tgtactggtg acgaaactca gtgttacggt acatgggttc
     5521 ctattgggct tgctatccct gaaaatgagg gtggtggctc tgagggtggc ggttctgagg
     5581 gtggcggttc tgagggtggc ggtactaaac ctcctgagta cggtgataca cctattccgg
     5641 gctatactta tatcaaccct ctcgacggca cttatccgcc tggtactgag caaaaccccg
     5701 ctaatcctaa tccttctctt gaggagtctc agcctcttaa tactttcatg tttcagaata
     5761 ataggttccg aaataggcag ggggcattaa ctgtttatac gggcactgtt actcaaggca
     5821 ctgaccccgt taaaacttat taccagtaca ctcctgtatc atcaaaagcc atgtatgacg
     5881 cttactggaa cggtaaattc agagactgcg ctttccattc tggctttaat gaggatccat
     5941 tcgtttgtga atatcaaggc caatcgtctg acctgcctca acctcctgtc aatgctggcg
     6001 gcggctctgg tggtggttct ggtggcggct ctgagggtgg tggctctgag ggtggcggtt
     6061 ctgagggtgg cggctctgag ggaggcggtt ccggtggtgg ctctggttcc ggtgattttg
     6121 attatgaaaa gatggcaaac gctaataagg gggctatgac cgaaaatgcc gatgaaaacg
     6181 cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc tactgattac ggtgctgcta
     6241 tcgatggttt cattggtgac gtttccggcc ttgctaatgg taatggtgct actggtgatt
     6301 ttgctggctc taattcccaa atggctcaag tcggtgacgg tgataattca cctttaatga
     6361 ataatttccg tcaatattta ccttccctcc ctcaatcggt tgaatgtcgc ccttttgtct
     6421 ttggcgctgg taaaccttac gagttcagta tcgactgcga taagatcaac ctgttccgcg
     6481 gtgtctttgc gtttctttta tatgttgcca cctttatgta tgtattttct acgtttgcta
     6541 acatactgcg taataaggag tcttaaaggg tccctaagtc tcctcagcaa aacgaaaggc
     6601 ccagtctttc gactgagcct ttcgttttat ttgaccggat gtcctcttcc tcctgggctg
     6661 acttcaggtg ctacatttga agagataaat tgcactgaaa tctagagcgg ttcagtagaa
     6721 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatcttttg ccctgtaaac
     6781 gaaaaaacca cctggggagg tggtttgatc gaaggttaag tcagttgggg aactgcttaa
     6841 ccgtggtaac tggctttcgc agagcacagc aaccaaatct gtccttccag tgtagccgga
     6901 ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc tgcgaactcc
     6961 cagttaccaa tggctgctgc cagtggcgtt ttaccgtgct tttccgggtt ggactcaagt
     7021 gaacagttac cggataaggc gcagcagtcg ggctgaacgg ggagttcttg cttacagccc
     7081 agcttggagc gaacgaccta caccgagccg agataccagt gtgtgagcta tgagaaagcg
     7141 ccacacttcc cgtaagggag aaaggcggaa caggtatccg gtaaacggca gggtcggaac
     7201 aggagagcgc aagagggagc gacccgccgg aaacggtggg gatctttaag tcctgtcggg
     7261 tttcgcccgt actgtcagat tcatggttga gcctcacggc tcccacagat gcaccggaaa
     7321 agcgtctgtt tatgtgaact ctggcaggag ggcggagcct atggaaaaac gccaccggcg
     7381 cggccctgct gttttgcctc acatgttagt cccctgctta tccacggaat ctgtgggtaa
     7441 ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc ggagcgtagc gaccgagtg
//
