LOCUS       PACE_AP_Pos_pBAD        6865 bp    DNA     circular SYN 24-JUN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Nicholas Southern
  TITLE     Direct Submission
  JOURNAL   Exported Jun 24, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6865
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="recombinant plasmid"
     CDS             complement(1..951)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the
                     pSC101 origin"
                     /label=Rep101
                     /note="color: #993366"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    955..1700
                     /label=SC101 Rep Origin
                     /note="color: #ffff00"
     rep_origin      999..1221
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
                     /note="color: #ffff00"
     misc_feature    1701..1715
                     /label=User Junction Modified Base
                     /note="color: #a6acb3"
     misc_feature    1716..1760
                     /label=rrnB1 transcriptional terminator
                     /note="color: #ffffff"
     misc_feature    1761..1767
                     /label=Nt-BbvCI
                     /note="color: #a6acb3"
     misc_feature    1768..1777
                     /label=User Junction Modified Base
                     /note="color: #a6acb3"
     misc_feature    1775..1777
                     /label=SanDI
                     /note="color: #a6acb3"
     misc_feature    1782..2178
                     /label=Geneious type: Concatenated sequence
                     /label=nonstandard type: Concatenated sequence
                     /note="color: #a6acb3"
     misc_feature    1788..1795
                     /label=PacI
                     /note="color: #a6acb3"
     promoter        1855..2139
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli;
                     thearaC regulatory gene is transcribed in the 
                     oppositedirection (Guzman et al., 1995)"
                     /note="color: #ffffff"
     protein_bind    1857..1873
                     /note="araO2"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    1994..2010
                     /note="araO1"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    2015..2031
                     /note="araO1"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    2036..2057
                     /note="CAP"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    2068..2084
                     /note="araI1"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    2089..2105
                     /note="araI2"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    2179..2186
                     /label=SbfI
                     /note="color: #a6acb3"
     misc_feature    2186..2191
                     /label=BsgI
                     /note="color: #a6acb3"
     misc_feature    2193..2205
                     /label=RBS
                     /note="color: #a6acb3"
     CDS             2206..3480
                     /codon_start=1
                     /label=Gene III
                     /note="color: #993366"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     CDS             3480..4559
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence
                     operon"
                     /note="color: #993366"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             4623..5603
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence
                     operon"
                     /note="color: #993366"
                     /translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
                     SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
                     SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
                     PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
                     HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
                     AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
     misc_feature    5606..5613
                     /label=PacI
                     /note="color: #a6acb3"
     misc_feature    5621..5627
                     /label=Nt-BbvCI
                     /note="color: #a6acb3"
     misc_feature    5628..5669
                     /label=tetA/orfL bidirectional terminator
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction
                     "
                     /note="color: #ffffff"
     misc_feature    5670..5682
                     /label=User Junction Modified Base
                     /note="color: #a6acb3"
     terminator      complement(5697..5743)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    5699..5743
                     /label=rrnB1 transcriptional terminator
                     /note="color: #ffffff"
     CDS             complement(5794..6654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc
                     Cleavage site after base 6585"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6655..6759)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
     1741 aagactgggc ctttcgtttt gctgaggaga cttagggacc catgccttta attaaggagt
     1801 tttgcaggtg ccttggaaca cctgcttttc gctgaattcg cggccgcttc tagaaagaaa
     1861 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
     1921 taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
     1981 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
     2041 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatccta
     2101 cctgacgctt tttatcgcaa ctctctactg tttctccatg accgaattca aaagatcttt
     2161 taagaaggag atatacatcc tgcaggtgca gtaaaggaaa aaaaaatgaa aaaattatta
     2221 ttcgcaattc ctttagttgt tcctttctat tctcactccg ctgaaactgt tgaaagttgt
     2281 ttagcaaaac cccatacaga aaattcattt actaacgtct ggaaagacga caaaacttta
     2341 gatcgttacg ctaactatga gggctgtctg tggaatgcta caggcgttgt agtttgtact
     2401 ggtgacgaaa ctcagtgtta cggtacatgg gttcctattg ggcttgctat ccctgaaaat
     2461 gagggtggtg gctctgaggg tggcggttct gagggtggcg gttctgaggg tggcggtact
     2521 aaacctcctg agtacggtga tacacctatt ccgggctata cttatatcaa ccctctcgac
     2581 ggcacttatc cgcctggtac tgagcaaaac cccgctaatc ctaatccttc tcttgaggag
     2641 tctcagcctc ttaatacttt catgtttcag aataataggt tccgaaatag gcagggggca
     2701 ttaactgttt atacgggcac tgttactcaa ggcactgacc ccgttaaaac ttattaccag
     2761 tacactcctg tatcatcaaa agccatgtat gacgcttact ggaacggtaa attcagagac
     2821 tgcgctttcc attctggctt taatgaggat ccattcgttt gtgaatatca aggccaatcg
     2881 tctgacctgc ctcaacctcc tgtcaatgct ggcggcggct ctggtggtgg ttctggtggc
     2941 ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc tgagggaggc
     3001 ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc aaacgctaat
     3061 aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc taaaggcaaa
     3121 cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg tgacgtttcc
     3181 ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc ccaaatggct
     3241 caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata tttaccttcc
     3301 ctccctcaat cggttgaatg tcgccctttt gtctttggcg ctggtaaacc ttacgagttc
     3361 agtatcgact gcgataagat caacctgttc cgcggtgtct ttgcgtttct tttatatgtt
     3421 gccaccttta tgtatgtatt ttctacgttt gctaacatac tgcgtaataa ggagtcttaa
     3481 tgaaatttgg aaactttttg cttacatacc aacctcccca attttcccaa acagaggtaa
     3541 tgaaacgttt ggttaaatta ggtcgcatct ctgaggagtg tggttttgat accgtatggt
     3601 tactggagca tcatttcacg gagtttggtt tgcttggtaa cccttatgtc gctgctgcat
     3661 atttacttgg cgcgactaaa aaattgaatg taggaactgc cgctattgtt cttcccacag
     3721 cccatccagt acgccaactt gaagatgtga atttattgga tcaaatgtca aaaggacgat
     3781 ttcggtttgg tatttgccga gggctttaca acaaggactt tcgcgtattc ggcacagata
     3841 tgaataacag tcgcgcctta gcggaatgct ggtacgggct gataaagaat ggcatgacag
     3901 agggatatat ggaagctgat aatgaacata tcaagttcca taaggtaaaa gtaaaccccg
     3961 cggcgtatag cagaggtggc gcaccggttt atgtggtggc tgaatcagct tcgacgactg
     4021 agtgggctgc tcaatttggc ctaccgatga tattaagttg gattataaat actaacgaaa
     4081 agaaagcaca acttgagctt tataatgaag tggctcaaga atatgggcac gatattcata
     4141 atatcgacca ttgcttatca tatataacat ctgtagatca tgactcaatt aaagcgaaag
     4201 agatttgccg gaaatttctg gggcattggt atgattctta tgtgaatgct acgactattt
     4261 ttgatgattc agaccaaaca agaggttatg atttcaataa agggcagtgg cgtgactttg
     4321 tattaaaagg acataaagat actaatcgcc gtattgatta cagttacgaa atcaatcccg
     4381 tgggaacgcc gcaggaatgt attgacataa ttcaaaaaga cattgatgct acaggaatat
     4441 caaatatttg ttgtggattt gaagctaatg gaacagtaga cgaaattatt gcttccatga
     4501 agctcttcca gtctgatgtc atgccatttc ttaaagaaaa acaacgttcg ctattatatt
     4561 atggcggtgg cggtagcggc ggtggcggta gcggcggtgg cggtagcggc ggtggcggta
     4621 gcaaatttgg attgttcttc cttaacttca tcaattcaac aactgttcaa gaacagagta
     4681 tagttcgcat gcaggaaata acggagtatg ttgataagtt gaattttgaa cagattttag
     4741 tgtatgaaaa tcatttttca gataatggtg ttgtcggcgc tcctctgact gtttctggtt
     4801 ttctgctcgg tttaacagag aaaattaaaa ttggttcatt aaatcacatc attacaactc
     4861 atcatcctgt ccgcatagcg gaggaagctt gcttattgga tcagttaagt gaagggagat
     4921 ttattttagg gtttagtgat tgcgaaaaaa aagatgaaat gcattttttt aatcgcccgg
     4981 ttgaatatca acagcaacta tttgaagagt gttatgaaat cattaacgat gctttaacaa
     5041 caggctattg taatccagat aacgattttt atagcttccc taaaatatct gtaaatcccc
     5101 atgcttatac gccaggcgga cctcggaaat atgtaacagc aaccagtcat catattgttg
     5161 agtgggcggc caaaaaaggt attcctctca tctttaagtg ggatgattct aatgatgtta
     5221 gatatgaata tgctgaaaga tataaagccg ttgcggataa atatgacgtt gacctatcag
     5281 agatagacca tcagttaatg atattagtta actataacga agatagtaat aaagctaaac
     5341 aagagacgcg tgcatttatt agtgattatg ttcttgaaat gcaccctaat gaaaatttcg
     5401 aaaataaact tgaagaaata attgcagaaa acgctgtcgg aaattatacg gagtgtataa
     5461 ctgcggctaa gttggcaatt gaaaagtgtg gtgcgaaaag tgtattgctg tcctttgaac
     5521 caatgaatga tttgatgagc caaaaaaatg taatcaatat tgttgatgat aatattaaga
     5581 agtaccacac ggaatatacc taaacttaat taacggcact cctcagcaaa tataatgacc
     5641 ctcttgataa cccaagaggg cattttttaa tgcccatggc gtagctgtgc tctagaataa
     5701 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgaccggaa atcagaattg
     5761 gttaattggt tgtaacactg gcagagcatt acgttaccaa tgcttaatca gtgaggcacc
     5821 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5881 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5941 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6001 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6061 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6121 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6181 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6241 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6301 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6361 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6421 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6481 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6541 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6601 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6661 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6721 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6781 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     6841 gaggccctta ggccaaatag gccgt
//
