LOCUS       2379_pJUMP27_pBA        4231 bp    DNA     circular UNA 01-JUL-2020
DEFINITION  Ligation of 2 sequences (Circularized).
ACCESSION   urn.local...2y-c3xyzmd
VERSION     urn.local...2y-c3xyzmd
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     source          order(<1287..4231,1..>62)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    47..>62
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     unsure          47..52
     misc_feature    <63..>66
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    63..66
                     /note="Geneious type: ligation"
     misc_feature    63..>352
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <63..66
                     /note="Geneious type: Concatenated sequence"
     source          <63..>66
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        68..352
                     /gene="araBAD"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the
                     oppositedirection (Guzman et al., 1995)"
     primer_bind     68..101
     source          <68..>352
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    68..352
                     /note="Geneious type: Editing History Replacement"
     primer_bind     275..294
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    complement(317..352)
                     /note="Geneious type: primer_bind_reverse"
     source          order(<353..>1066,<1100..>1285)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     unsure          367..383
     CDS             order(392..>1066,<1100..1102)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDG
                     GVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGE
                     IKMRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAE
                     GRHSTGA"
     unsure          complement(516..535)
     unsure          544..563
     misc_feature    1067..1099
                     /note="Geneious type: Tag"
     primer_bind     1100..1128
     misc_feature    1100..1099
                     /note="Geneious type: Concatenated sequence"
     terminator      1135..1206
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1222..1249
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1262..1281)
     misc_feature    1287..1290
                     /note="Geneious type: ligation"
     misc_feature    <1287..1291
                     /note="universal suffix for all parts"
     unsure          <1287..1291
     terminator      1325..1419
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1540..1559)
                     /note="pENTR vectors, reverse primer"
     CDS             1551..2366
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin in bacteria or G418
                      (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2533..2641
                     /note="incP origin of transfer"
     CDS             complement(2652..3602)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFDIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3650..3872
     terminator      4139..4225
                     /gene="Escherichia coli rrnB"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN      
        1 ttaattaagg agttttgcag gtgccttgga acacctgctt ttcgctgaat tcgcggccgc
       61 ttctagaaag aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac
      121 tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc
      181 gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc
      241 cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga
      301 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc atgaccgaat
      361 tcaaaagatc ttttaagaag gagatataca tatggcgagt agcgaagacg ttatcaaaga
      421 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga
      481 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa
      541 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa
      601 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg
      661 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga
      721 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc
      781 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat
      841 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg
      901 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc
      961 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat
     1021 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgctgctg ctaacgacga
     1081 aaactacgct ttagtagctt aaggatccaa actcgagtaa ggatctccag gcatcaaata
     1141 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1201 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatacctag
     1261 ggatatattc cgcttcctcg ctcacctgca gggagttgtc ttcgaagact tcgctctagt
     1321 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     1381 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac
     1441 gatttaaatt tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     1501 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     1561 ttcagcgtga aacgagctgt agccgtccgc gtctgaacag caacatggat gcggatctgt
     1621 atggctataa atgggcgcgt gataacgtgg gtcagagcgg cgcgaccatt tatcgtctgt
     1681 atggcaaacc ggatgcgccg gaactgtttc tgaaacatgg caaaggcagc gtggcgaacg
     1741 atgtgaccga tgaaatggtg cgtctgaact ggctgaccga atttatgccg ctgccgacca
     1801 ttaaacattt tattcgcacc ccggatgatg cgtggctgct gaccaccgcg attccgggca
     1861 aaaccgcgtt tcaggtgctg gaagaatatc cggatagcgg cgaaaacatt gtggatgcgc
     1921 tggccgtgtt tctgcgtcgt ctgcatagca ttccggtgtg caactgcccg tttaacagcg
     1981 atcgtgtgtt tcgtctggcc caggcgcaga gccgtatgaa caacggcctg gtggatgcga
     2041 gcgattttga tgatgaacgt aacggctggc cggtggaaca ggtgtggaaa gaaatgcata
     2101 aactgctgcc gtttagcccg gatagcgtgg tgacccacgg cgattttagc ctggataacc
     2161 tgattttcga tgaaggcaaa ctgattggct gcattgatgt gggccgtgtg ggcattgcgg
     2221 atcgttatca ggatctggcc attctgtgga actgcctggg cgaatttagc ccgagcctgc
     2281 aaaaacgtct gtttcagaaa tatggcattg ataatccgga tatgaacaaa ctgcaatttc
     2341 atctgatgct ggatgaattt ttctaataat taattggacc gcggtccgcg cgttgtcctt
     2401 ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt
     2461 tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca
     2521 acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt
     2581 cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc
     2641 gtaggccggc ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt
     2701 ttttgcgtga gccatgagaa cgaaccattg agatcatact tactttgcat gtcactcaaa
     2761 aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt
     2821 cttagtccgt tatgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc
     2881 atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact
     2941 tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg
     3001 taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca
     3061 tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt
     3121 gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag
     3181 tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg
     3241 aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg
     3301 gcatagtttg tccactggaa aatatcaaag cctttaacca aaggattcct gatttccaca
     3361 gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg
     3421 atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta
     3481 gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc
     3541 tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac
     3601 atacatctca attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa
     3661 tgataattac atgtcctttt cctttgagtt gtgggtatct gtaaattctg ctagaccttt
     3721 gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt
     3781 ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata
     3841 aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac
     3901 aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc
     3961 ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat
     4021 caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc
     4081 agtgaatggg ggtaaatggc actacaggcg cggcgcgccc agctgtctag ggcggcggat
     4141 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4201 tcgactgagc ctttcgtttt atttgatgcc t
//
