LOCUS       sd2_AraC_S170_LO        4966 bp    DNA     circular SYN 24-JUN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Jun 24, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4966
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="recombinant plasmid"
     primer_bind     154..171
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             complement(379..1461)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1423..1442
                     /label=LacI, reverse primer
                     /note="LacI, reverse primer"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        complement(1462..1539)
                     /gene="lacI"
                     /label=lacI promoter
                     /note="color: #ffffff; direction: LEFT"
     promoter        1584..1613
                     /note="strong E. coli promoter; hybrid between the trp
                     andlac UV5 promoters"
                     /note="color: #ffffff"
     protein_bind    1621..1637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     misc_feature    1645..1658
                     /standard_name="SD4 RBS"
                     /label=SD4 RBS
                     /label=sd2 RBS
                     /note="color: #a6acb3"
     misc_feature    1659..2975
                     /label=AraC
                     /note="color: #993366"
     variation       1668..1670
                     /label=E3K
                     /note="color: #a6acb3"
     misc_feature    1668
                     /label=G to A
                     /note="color: #a6acb3"
     variation       1710..1712
                     /label=Silent Mutation
                     /note="color: #a6acb3"
     misc_feature    1712
                     /label=G to A
                     /note="color: #a6acb3"
     variation       1926..1928
                     /label=Silent Mutation
                     /note="color: #a6acb3"
     misc_feature    1928
                     /label=C to A
                     /note="color: #a6acb3"
     variation       2097..2099
                     /label=R146L
                     /note="color: #c0c0c0"
     misc_feature    2098
                     /label=G to T
                     /note="color: #a6acb3"
     variation       2115..2117
                     /label=A152T
                     /note="color: #a6acb3"
     misc_feature    2115
                     /label=G to A
                     /note="color: #a6acb3"
     misc_feature    2117
                     /label=G to A
                     /note="color: #a6acb3"
     motif           2172..2174
                     /label=linker
                     /note="/created_by=User"
                     /note="color: #a6acb3"
     CDS             2175..2597
                     /label=LOV2_ecoli
                     /note="/created_by=User"
                     /note="color: #993366; direction: RIGHT"
     motif           2598..2603
                     /label=linker
                     /note="/created_by=User"
                     /note="color: #a6acb3"
     variation       2715..2717
                     /label=Silent Mutation
                     /note="color: #c0c0c0"
     misc_feature    2717
                     /label=G to T
                     /note="color: #a6acb3"
     variation       2844..2846
                     /label=R251L
                     /note="color: #c0c0c0"
     misc_feature    2845
                     /label=G to T
                     /note="color: #a6acb3"
     misc_feature    2976..2978
                     /standard_name="pr"
                     /label=pr
                     /note="color: #a6acb3"
     misc_feature    2983..2998
                     /standard_name="retained"
                     /label=retained
                     /note="color: #a6acb3"
     misc_feature    2999..3001
                     /standard_name="J23107 promoter"
                     /label=J23107 promoter
                     /note="color: #a6acb3"
     primer_bind     complement(3016..3033)
                     /note="For vectors with E. coli araBAD promoter,
                     reverseprimer"
                     /note="color: #a020f0; direction: LEFT"
     terminator      3186..3272
                     /gene="Escherichia coli rrnB"
                     /label=Escherichia coli rrnB terminator
                     /note="transcription terminator T1 from the E. coli
                     rrnBgene"
                     /note="color: #ffffff"
     terminator      3364..3391
                     /note="transcription terminator T2 from the E. coli
                     rrnBgene"
                     /note="color: #ffffff"
     terminator      3411..3505
                     /note="transcription terminator from phage lambda"
                     /note="color: #ffffff"
     terminator      complement(3411..3505)
                     /note="transcription terminator from phage lambda"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     3411..3442
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(3488..3516)
                     /note="color: #a020f0; direction: LEFT"
     gene            complement(3526..4185)
                     /gene="CAT/CamR"
                     /label=CAT/CamR
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(3526..4185)
                     /codon_start=1
                     /note="color: #993366"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(3749..3754)
                     /gene="CAT/CamR"
                     /label=CAT/CamR
                     /note="color: #a6acb3; direction: LEFT"
     marker          complement(3749..3754)
                     /direction=LEFT
                     /label=Geneious type: marker
                     /note="color: #a6acb3"
     misc_feature    3749..3754
                     /label=Geneious type: Concatenated sequence
                     /label=nonstandard type: Concatenated sequence
                     /note="color: #a6acb3"
     CDS             complement(3749..3754)
                     /codon_start=1
                     /note="color: #993366"
                     /translation="MF"
     primer_bind     4100..4119
                     /label=Chloramphenicol resistance gene, reverse primer
                     /note="Chloramphenicol resistance gene, reverse primer"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        complement(4186..4288)
                     /note="promoter of the E. coli cat gene
                     encodingchloramphenicol acetyltransferase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     4289..4327
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     4867..4886
                     /label=pBR322 origin, forward primer
                     /note="pBR322 origin, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgccacg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
      241 ctcaacgaca ggagcacgat catgctagtc atgccccgcg cccaccggaa ggagctgact
      301 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
      361 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      421 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
      481 ttttcttttc accagtgaca cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
      541 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
      601 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
      661 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
      721 atcgttggca accagcatcg cagtgggaac gataccctca ttcagcattt gcatggtttg
      781 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
      841 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
      901 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
      961 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     1021 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     1081 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     1141 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     1201 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     1261 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     1321 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     1381 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     1441 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
     1501 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct
     1561 tatgcgactc cgatcccgca aatttgacaa ttaatcatcc ggctcgtata atgtgtggaa
     1621 ttgtgagcgg ataacaattg gagtaaaaaa aaaggaaaat gtccgcgaaa gcgcagaacg
     1681 atccgctgct gccgggctat agctttaacg cacatctggt ggcgggcctg accccgattg
     1741 aagcgaacgg ctatctggat ttttttattg atcgcccgct gggcatgaaa ggctatattc
     1801 tgaacctgac cattcgcggc cagggcgtgg tgaaaaacca gggccgcgaa tttgtgtgcc
     1861 gcccgggcga tattctgctg tttccgccgg gcgaaattca tcattatggc cgccatccgg
     1921 aagcgcgaga atggtatcat cagtgggtgt attttcgccc gcgcgcgtat tggcatgaat
     1981 ggctgaactg gccgagcatt tttgcgaaca ccggcttttt tcgcccggat gaagcgcatc
     2041 agccgcattt tagcgatctg tttggccaga ttattaacgc gggccagggc gaaggcctct
     2101 atagcgaact gctgacaatt aacctgctgg aacagctgct gctgcgccgc atggaagcga
     2161 ttaacgaaag caaattagcc acaacgctgg aacgcattga aaagaatttc gtaatcacag
     2221 acccgcgcct tcccgacaat ccaattattt ttgcgtccga tagcttcctg caattaaccg
     2281 aatacagccg cgaagaaatt ctgggtcgta attgtcgctt ccttcagggg ccagagactg
     2341 accgtgctac ggtacgcaaa atccgcgacg caatcgacaa tcaaacggaa gtcacggttc
     2401 agttgattaa ctatacgaag agcggaaaaa aattctggaa tttatttcac ttgcagccta
     2461 tgcgtgacca gaagggcgat gtccagtatt tcattggcgt tcagcttgat ggtaccgagc
     2521 atgttcgcga tgctgcggag cgtgaaggtg taatgttaat taaaaagact gctgaaaaca
     2581 ttgatgaggc ggccaaaggg agcctgcatc cgccgatgga taaccgcgtg cgcgaagcgt
     2641 gccagtatat tagcgatcat ctggcggata gcaactttga tattgcgagc gtggcgcagc
     2701 atgtgtgcct gagccctagc cgcctgagcc atctgtttcg ccagcagctg ggcattagcg
     2761 tgctgagctg gcgcgaagat cagcgcatta gccaggcgaa actgctgctg agcaccaccc
     2821 gcatgccgat tgcgaccgtg ggcctcaacg tgggctttga tgatcagctg tattttagcc
     2881 gcgtgtttaa aaaatgcacc ggcgcgagcc cgagcgaatt tcgcgcgggc tgcgaagaaa
     2941 aagtgaacga tgtggcggtg aaactgagcg ggtaaggcta atggagattt tcaacatggg
     3001 cgagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     3061 gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     3121 gaaacgccgt agcgccgatg gtagtgtggg gtatccccat gcgagagtag ggaactgcca
     3181 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     3241 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     3301 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
     3361 agcagaaggc catcctgacg gatggccttt tctgaaagga ggaactatat attctcacca
     3421 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     3481 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     3541 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     3601 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     3661 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     3721 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
     3781 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     3841 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     3901 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     3961 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     4021 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     4081 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     4141 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     4201 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     4261 aaagttggaa cctcttacgt gccgatcaac agttttattg ttcatgacca aaatccctta
     4321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcaag
     4381 ggatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4441 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4501 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4681 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4741 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4801 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4861 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4921 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaa
//
