LOCUS       Exported                8342 bp ds-DNA     circular SYN 01-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Cody Kime
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 1, 2025 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     36..57
                     /label=Amp-pro-AS (11-16)
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F (7-72)
                     /note="F1 origin, forward primer"
     primer_bind     728..744
                     /label=M13F (5-10)
     primer_bind     812..831
                     /label=BII
                     /label=BII(R)
     repeat_region   join(874..886,918..919,921..936)
                     /label=5'TR
     repeat_region   887..917
                     /label=5'TR
     misc_feature    887..917
                     /label=31bp spacer
     enhancer        1113..1343
                     /label=Chicken beta-globin core insulator
                     /label=core insul(1)
     promoter        1385..1698
                     /label=mU6 promoter
                     /note="Common feature added by Mo Mandegar"
     primer_bind     1499..1521
                     /label=mU6-S115 (2-14)
     protein_bind    1579..1612
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    1579..1612
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     1682..1753
                     /label=MM BsmBI Uni F
     misc_feature    1689..1692
                     /label=CACC
     primer_bind     1700..1718
                     /label=hSOX2 sgRNA-1
     misc_feature    1719..1811
                     /label=modified stem loop
                     /note="Termination signal = 7 Ts at the end.
                     Common feature added by Mo Mandegar"
     misc_feature    1719..1722
                     /label=GTTT
     terminator      1875..1885
                     /label=3X STOP
                     /note="Common feature added by Mo Mandegar"
     enhancer        1956..2259
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        2261..3508
                     /label=CAG
                     /note="Common feature added by Mo Mandegar"
     promoter        2261..2538
                     /label=chicken beta-actin promoter
     primer_bind     3481..3502
                     /label=CAG-F (5-18)
     intron          3516..3606
                     /label=Mini-Intron
                     /note="Mini Intron = Rabbit Globin Intron
                     Common feature added by Mo Mandegar"
     misc_feature    3612..3621
                     /label=Kozak+ATG
                     /note="Common feature added by Mo Mandegar
                     
                     Note: Designating ""cgccaccATG"" as the Kozak+ATG in a 
                     number of constructs. This is the most conserved consensues
                     sequence. Works well in HEK and iPS cells.
                     
                     From Wikipedia Dec 2013:
                     The Kozak consensus sequence, Kozak consensus or Kozak 
                     sequence, is a sequence which occurs on eukaryotic mRNA and
                     has the consensus (gcc)gccRccAUGG. The Kozak consensus 
                     sequence plays a major role in the initiation of the 
                     translation process.[1] The sequence was named after the 
                     person who brought it to prominence, Marilyn Kozak."
     CDS             3622..3642
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3646..3666
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3670..3690
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3699
                     /label=Mu
                     /note="Mutated (silent) this to remove the BstXI from 
                     Original Seq
                     Still codes for a Serine
                     
                     Mo Mandegar"
     CDS             3706..4422
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3751..3772)
                     /label=EGFP-N (11-78)
                     /note="EGFP, reverse primer"
     primer_bind     3969..3990
                     /label=EGFP-S264 (5-28)
     primer_bind     complement(4063..4084)
                     /label=EGFP-AS357 (5-34)
     primer_bind     4359..4380
                     /label=EGFP-C (5-36)
                     /note="EGFP, forward primer"
     CDS             4423..4476
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4477..4899
                     /codon_start=1
                     /product="Bacillus subtilis Blasticidin resistance gene"
                     /label=BSR
                     /label=Bsd
                     /note="Common feature added by Mo Mandegar"
                     /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS
                     AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR
                     ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     polyA_signal    4912..5033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4949..4968)
                     /label=SV40pA-R (2-72)
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5216..5237)
                     /label=PB-3prime-AS (6-17)
     enhancer        complement(5291..5521)
                     /label=Chicken beta-globin core insulator
                     /label=core insul(1)
     repeat_region   5796..5830
                     /label=3'TR
     primer_bind     complement(6321..6337)
                     /label=M13R (5-11)
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    6345..6361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(6349..6368)
                     /label=pLac
                     /label=pLac(F)
     promoter        complement(6369..6399)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6414..6435
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6723..7311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6803..6822)
                     /label=pBR322ori-F (10-49)
                     /note="pBR322 origin, forward primer"
     CDS             complement(7482..8342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7931..7953
                     /label=Amp-AS390 (2-52)
     primer_bind     complement(8102..8125)
                     /label=Amp-S218 (2-51)
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggcccgat gaggcccttt cgtcttcact
     1381 cgaggatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacgga cttgtgggag
     1441 aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag taactataga
     1501 ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct acattttaca
     1561 tgataggctt ggatttctat aacttcgtat agcatacatt atacgaagtt ataaacagca
     1621 caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat aagtatccct
     1681 tggagaacca ccttgttggc ctgacagccc ccgtcacagt ttaagagcta tgctggaaac
     1741 agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     1801 gtgctttttt tctcgagtac taggatccat taggcggccg cgtggataac cgtattaccg
     1861 ccatgcatcc cgggtgactg actgagcggc cgcactagtt attaatagta atcaattacg
     1921 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1981 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2041 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2101 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2161 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2221 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     2281 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     2341 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg
     2401 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
     2461 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     2521 cgaagcgcgc ggcgggcggg gagtcgctgc gacgctgcct tcgccccgtg ccccgctccg
     2581 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     2641 ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt
     2701 cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga
     2761 gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc
     2821 tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc
     2881 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca
     2941 aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg
     3001 ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg
     3061 cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
     3121 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
     3181 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     3241 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     3301 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     3361 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     3421 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     3481 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     3541 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     3601 gcaaagaatt ccgccaccat gccaaaaaag aagagaaagg tagatccaaa aaagaagaga
     3661 aaggtagatc caaaaaagaa gagaaaggta cacgtgtcaa cgcgtatggt gagcaagggc
     3721 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3781 cacaagttca gcgtccgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg
     3841 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     3901 ggctacggcg tggcctgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3961 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tctctttcaa ggacgacggt
     4021 acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4081 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4141 ttcaacagcc acaacgtcta tatcacggcc gacaagcaga agaacggcat caaggctaac
     4201 ttcaagatcc gccacaacgt tgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4261 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagccatcag
     4321 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4381 accgccgccg ggattacaca tggcatggac gagctgtaca aggagggcag aggaagtctt
     4441 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga agaccttcaa catctcccag
     4501 caggatctag aattagtaga agtagcgaca gagaagatta caatgcttta tgaggataat
     4561 aaacatcatg tgggagcggc aattcgtacg aaaacaggag aaatcatttc ggcagtacat
     4621 attgaagcgt atataggacg agtaactgtt tgtgcagaag ccattgcgat tggtagtgca
     4681 gtttcgaatg gacaaaagga ttttgacacg attgtagctg ttagacaccc ttattctgac
     4741 gaagtagata gaagtattcg agtggtaagt ccttgtggta tgtgtaggga gttgatttca
     4801 gactatgcac cagattgttt tgtgttaata gaaatgaatg gcaagttagt caaaactacg
     4861 attgaagaac tcattccact caaatatacc cgaaattaag ttaactaact aaacttgttt
     4921 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4981 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5041 tggaattgac tcaaatgatg tcaattagtc tatcagaagc tcatctggtc tcccttccgg
     5101 gggacaagac atccctgttt aatatttaaa cagcagtgtt cccaaactgg gttcttatat
     5161 cccttgctct ggtcaaccag gttgcagggt ttcctgtcct cacaggaacg aagtccctaa
     5221 agaaacagtg gcagccaggt ttagccccgg aattgactgg attccttttt tagggcccat
     5281 tggtatggct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     5341 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
     5401 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
     5461 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc
     5521 tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat
     5581 aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc
     5641 attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca
     5701 cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc
     5761 gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat
     5821 ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag
     5881 tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg
     5941 cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga
     6001 gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg
     6061 tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt
     6121 ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg
     6181 ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga
     6241 cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg
     6301 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6361 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6421 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6481 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6541 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6601 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6661 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6721 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6781 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6841 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6901 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6961 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7021 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7081 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7141 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7201 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7261 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7321 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7381 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7441 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7501 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7561 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7621 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7681 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7741 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7801 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7861 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7921 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7981 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8041 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8101 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8161 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8221 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8281 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8341 at
//
