LOCUS       Exported               15764 bp ds-DNA     circular SYN 25-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 1, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15764
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    969..971
                     /label=PAM mutation
     RBS             5993..6001
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    10726..10860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11181..11197)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11671..11775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11776..12636
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12807..13395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    13683..13704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13719..13749
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13757..13773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13781..13797
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    14061..14142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(14259..14657)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(14658..14714)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(14724..15467)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(15468..15491)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      15491..15500
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(15520..15731)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccgcggggag gggcggggtc tcgatcgcgc tattgtcatg
      421 gagacgggaa gctggctgca gcggcggcgg ggaccgtggg gccgaggtgg ctgccagccg
      481 gccaatgtct aagcgaggcg gagcggccca ggcggcccga gcctggggga gcgcgcagcc
      541 ggccagtggc ggcctcgccg gcggcctctt cccgggctcg cagtaggccc gagtcgtcgc
      601 cgggagctcc tgggagcagc gtccccgccc tgctcccctc gctcccgcct cttgcggccc
      661 cacggcccct cagcgcccgc ccccggctcc gcccgccgca gccgcagccc ctggcgctaa
      721 cggtcggtaa cggcccgcgc gcgccgcccg ccgggggctc gcgccagcca cgagggagcg
      781 tccgcggccc gcgcgcccgc gcggcggagg agaggtgagc ccccgcccgg gccaggccct
      841 ctggccgcgc cgtccgcccc tctagtcgtg tcccctcgtg ggccgaacgg acgcggcggt
      901 gccccgcgcc cgaccagacg tcccgtgggc tagggcctgg gcctcgggcc gcgtcggcgc
      961 cggtcgaggt tctccgggtg tcggggttcg gggcgggcgc gcgtgggcgt ggctcctctg
     1021 tccacgcctg ttcccttcgt cgccgcggct ctcgtccggg acacggcttt ccggagtaga
     1081 gcccttggag gtttgtgcca ccgagaaccc gagtctgtca cacagacatc ctcattacat
     1141 catcctgcca ctccggcagt cccgcgcttt ctccccccac ccccgccccc gcccccgccc
     1201 ccgccccgtg gcttgtttgt tggttgtttt tttaattttt ttaacccctt ttcttgtact
     1261 gtcttctttt tggtgtcagg ggctggagag actcctgcaa gatattgagg catttagaat
     1321 gtatggttct gttgccgtgg ttgtcacctc cgctctacgc caaatttaag actcatttct
     1381 taaggatgtc tctattttct gctttatttg cacattgatg gctctgctgc tcagctggcc
     1441 acggccgccc ggcgtctgag atagaattgt gttaatatga gaaagggaac ggccgatcat
     1501 gattagcagg cagacggcat gcagatagca tctggcgggt tttctccgtt tttatatgaa
     1561 agcgttcact tgtttgctgg taaacacgag gttttaactt tttatctggg tagtggtgac
     1621 cgtttgtttc actctcccct cctttttact tcctattcat tagcttttcc gtttggaaag
     1681 ctgcatactt gttagcggta gtttggtgtc ttgactctga agctttctct tccagcagag
     1741 gtgggatctg tatccctccc atcctaggtt accaagaaga ctttttctcc ccttactttc
     1801 tattgaattt gtgtctttcc caacatttaa acttacacta gggataagtt tgctcgggtt
     1861 gtttttgacg taggactatt aaatattctc attttgaaac ttcctaaagg tttattggtt
     1921 tgtcatgtct gcaccgatcc agtttcgcat aactcttaaa atttaaagct ttaaagtacg
     1981 gttcgcaatt gtattgtcaa gacatggccc tagagcttgt tcttcaagtt ttggctgtac
     2041 agttgtcatt gtttcagtca agtgcaaaag aggttattcc tgtttattta gtgttaatag
     2101 ctatttacag ttaaaacttt aaagactcat agaagtaggt ctgctgtatt ttaaagatct
     2161 catcattctt gtgggagaga cacaacacca acaccaggaa aggcaacaga ttgcttctgt
     2221 catatcgctt tgctgttttg gagtgtactt aaaggattat gattgataag gctgtcttgc
     2281 cagagaatgt tgacacaggt gtgcatcgtt atcttaatta agatcctgag aaaatgttgt
     2341 ctataaatgg tgacataaga agggcggcat atttttgtac agttttgctt ttggagaatt
     2401 taattgcact tgaaacctgt ctttgagctt ccagattttg tgagcacttt agcaacagaa
     2461 gttttagaga gaaatgatag tttaatgtta agtgttctgg catgtactaa tactacttgt
     2521 ggctttttgg caatgcctgt gtcggctttc tttgtgctct acctttgtgg aataatggtt
     2581 tctaattttg aaagaaaagc ataaaataga gcagtatttt attaagcaaa cacttgttag
     2641 tgttcagaat aagtctgtag aggagggcat gcttaatgtg cagaaacagg aatataataa
     2701 gaggtaaata tatgagaaat ttatgctatt tcgttttttt ggaaaagaaa cagtctcctg
     2761 gggtgcctta atgatctaaa acaatttctt tttcatatca ttaaatacct agatacaatg
     2821 tttacggaat gccttgtttt tatttctaaa aatagaagaa aactgtttta tttcttccaa
     2881 aatactgttc tgtgtgtttg aattgatgag taagtgaaga tagtgcattt agtctcttac
     2941 tagtttaagg atctgagtca taacctgggt gaccactaaa ataatattgc tgttctttac
     3001 tacaaatctc ggaatgcaaa aacactggca agatggatgc tgttgatgat attacccttg
     3061 gcttacccag cacctaatac cttttaacat aagtattgca ttaagatttt ctcttctctt
     3121 tgtagcttag gatttcctgg aagctggctg tctatcagtg tgtatgtaag aagtgtgtat
     3181 gtttttcttt aatattaaaa gtcactgtaa aaagaggaaa tgaaagtgga ggagaccata
     3241 ggcatatgat cggaaaagcc aggtgtagag tcacagctca ttcttgactc caaggataat
     3301 tacctggtta gaattacatg gtaaactaaa agaaagactt acgtagattt gtttgctaaa
     3361 actaaaacaa gaccattaat tttgagataa ttgctttatt tatctcccat accaccccta
     3421 atcaaaattt ttttcccctc tggcagctat gatgagcatt cttatgctgt tatccatgtg
     3481 ttattttcaa atttgatata ttgcattaaa agagatgaaa taggaatatg aactaattat
     3541 ccattttaac gaataactca ataattatta ttatgtgtat acattctaga aatcttagga
     3601 aaaaatgttc tttagttgat aacaggaaag taaattaaat catgtaatcc tgagtgaatt
     3661 aaatatgctc ttcaaaatag atgatgaaca tagcatcata ctttggaata atgttttaac
     3721 tctgggatag tacagcctgt tttgagtggt agtatttcat tatctttata tgtttctctt
     3781 caatatctct tttttgttga ctcctatatt ttaacatctt tttggtatat ttgttagctg
     3841 tttataggga ataatttttc ttaatagaat agcagctggt gcaactctaa gaacatgtca
     3901 aatctaggaa ttcttttttc ctttttcaat aatgagtggt ttgctttcat agcactgctc
     3961 tgaagtgtga tgctgaaggc ttatttctgc ataaatctgc agtgttttta tagctgtagt
     4021 gtacctaagc accaatttag cttagcaaat taacttagat gcttcaaaat tgaaaaccag
     4081 tatattctaa tgtagtttga gtttaagtct ttattagtaa ggatgcactt aacatttgtt
     4141 caggagaaga gaatataatt ttctcctgca gttgtcattt acgtaatcca tttttctagt
     4201 cttttctcag gctaatgata ttccatacct tgatacagct tttatttgtt tatttgccat
     4261 aatatggagt gataccttcg agaaaattag atatttgaat ctaatatccc aaaagtatta
     4321 cttagttttc tgtgtttcct taaacagaat catttccatt ttcggtgcag gtgttaagtg
     4381 tgatgcttcc ataatacatt tggatgctgt cagctaagtt cacttctgaa ctaaggggtt
     4441 cctccaaatg ttggctgaaa ttcatcccaa ggctggtctg caagtgagtg tctgcacaca
     4501 gtttgcttgt atgtggagtc gatccaaaat agcatcaatg ttggttttac caaagtattt
     4561 attattgata atagaggcta agtacaaaat gtagagaatg tcagctactt gaggcctttg
     4621 attattaaaa attttattaa tgcattaaac aagagtacag taaatagata aattttaggt
     4681 tcatgaaata aaactgaata atttattttt acttactatt tatcatggaa ttactttgaa
     4741 taatttattt ttaatggtat aattggacag taaaatttat aaactcagtg cttttcataa
     4801 aaatcaaagt gaagtttgta atattttata caaatagaat tattatttaa gagaaataac
     4861 ctgtttatgc ctaattacag tttttaatca tttcagttac ctatttcttt taagaataaa
     4921 tttagtggga atatcagttc cagtcatggg taccaaactt ttttagtgac agagtacaca
     4981 caggtatgta aaacttgtca tttctcatca aatagaggct gctgaatata ggcaggtaag
     5041 aaaagctatg agaaagaatt gttttgcaga atatttgtct agttgtcaga gcaaggaact
     5101 gaatttattg acacagaaca ttttattaag taaaaaaaat ggttcttata acaaaaaaaa
     5161 aagctaattt tacagaaggc agtagttaat atagccgcca ataaaataaa tgtttctctg
     5221 aatactttcc gaggcattac agtgattttt aactaatatg aagtgatata taataatttt
     5281 aaagtaacat ccttgagttt tcctattatt aattgcttat ggaaattggg tttcacgtat
     5341 gactgagagc taaagcatta cagtgagtta gaaaacacaa cacaaatgta aagaaaatgt
     5401 taggtggtga gtaatctgat tcctttttgt ttgcctttaa gcttagtttt ttgttttgtt
     5461 ttactttgtt ttaaaccatg tataaaattg ttgaatttaa aagataagag gattaagtaa
     5521 tttctttttt cctcctaagg ataaatgtag gaaaaatcta aacacatagg cagattggtt
     5581 gagttttata tctgttatcg gccacattta ttaagattca tatttcatgt atattagagg
     5641 tattcacatg tattaaaatt cttatattcc ttctatataa aatagatgta gggggttccc
     5701 actcttgaaa atatgaagaa aagatgtcct ttcagcaata atgggttatg gttgattaac
     5761 tgagaaggtt gtattaaacg ttctctagta gaaatggcta aagagcatgc tttttgagaa
     5821 gtgtatcatc taggaagaaa atcaaatgga gtattggtaa ttaaattgta attccatgaa
     5881 ggaaggaagt ggtgcaaaag atgaagctaa ctattcctgt ttttcttttt aagagtctgc
     5941 aattcataat ggagctactg tactggctat tggaaggagg agattctgaa gataaggagg
     6001 taatattatc tcttttaaaa gaatactttc ctctgtaatc ctgaatcttt attacatgta
     6061 agaactttgt gcagtagaca gcaatttctt tgaatttggt atatggaaac aattttattt
     6121 tcctctgcta agtttttgag cctgcctctt ctagtgccat ggactgcatt ggtagagctg
     6181 agaaatatca tttagccata ctcagcaccc ttaaaatagc ttctttctga gaattagatc
     6241 tgtgaaggtg tcctgcacag ttcttgtaga tgtcatttta gtttgtggtt gacgtgcatg
     6301 catttagcat gttgcttaac cgtcctcatt cgcctcccag ttctttgttg ccttcatttg
     6361 gggggatgtg ttttctgctg gatgatttac tgctagacat gaccaaactc tgagtataag
     6421 acttggtgtt tggcagctgg ttccagtgct ctgctgagaa gtagttgggc ccagcctggg
     6481 gcattgtagt gtcctggagg ccgggagctc ctgcacaggg gttcttttcc tggttcagag
     6541 ccttaggtgc ctttccactt ttcacgtaat cttttcctag gttggcttgc tgcttacttt
     6601 gcagctgttg cagggattca catggaatgg agggctccct tttattgtgg attatttctt
     6661 tgataattta cccatgtgct ttttcatttt caaaaaccca gtggtgttta agaatgaaag
     6721 attcttgaag aacaaaaatt gggttaaatt tgtatctatc agaaaagatt ctatcctctg
     6781 atgctatgta ggcacattga atatgcaggc taaattaaaa acagagtaaa acctttttat
     6841 aatatgccaa ttactatatc taagaatgtt tatacaggct gaaattttgt aatggtattt
     6901 atatctgttt tcctttttaa ggaaaataat atacttttct aggcatcaaa aatatctgcc
     6961 cctttcacta atgctgatgt accaccttcc cccaaccccc aacgctaaat ttgactggct
     7021 taaaaacatc tgccccctga actatatctg gggaggaata ttaataaaac aatgaaggac
     7081 ttcatggtgt ctagttatat aattaggaaa ttggttagaa acgtggctaa aaccagtttc
     7141 tttcattaaa agaactagtg tacatttaaa aacaaaaacc ttcaggaaaa agactctttg
     7201 atctttaagt caaatagtat ttttggttaa cacagcagga aggggaaata taccaatttc
     7261 agattctttt atttatgcct aagtagaggt tgtaagcagc taaggaagtg attaaaatgt
     7321 agtctagtaa aaaatggtgc tgattacttg gaaagcagtt tacatttgca aaaaaattca
     7381 gtattatgac cttcaccaac ttttacacat catatacttg ggttattact tatggtatag
     7441 cttgtgtatg gaatgcaagg gtattttcaa attgactagg tcttgctggt catcttaaaa
     7501 tatgttacaa tattacaaaa ttgaaatgta atatttttta atagaaggaa aatataaatt
     7561 taatatctgg gcaattgaga cctttaaact tactttaaaa gtatgatctt gatgtatatg
     7621 atactgtttt gtctttgcta tattaacaga attagagggg tgttctgcaa ttcaaatacc
     7681 ttatatattc caaattttat tctctataat ggacttttaa aataaaaggt atatgtgctt
     7741 caagagggca aaatttgaat catgagctaa tttgctaagc atcagattat agaaaagcat
     7801 ccttgattaa tttggaactg tgaaaggggg cgggtaaaac tgttttctgc agaaatttac
     7861 tagtgcagca accatttaaa ttaaatgttt gttaacataa tagtgatggc attttctcct
     7921 ccccctcctt gtggttttgt ccaactagat gttacagtgg cagttgcact gactgttaag
     7981 tgtttaaatg atgacaccat tatgtgaagt gattttgaaa tgagagattc cagccaagaa
     8041 ttacatctgc tcccatctcc ttcaaatcat actctctggc agtacagatt atgattgatt
     8101 tgtttgtgac agattgcagg aaacagtcat tgatttttca atattttacc ttaaaattat
     8161 ttacagttgt aaccatgggg aggtattttc atgggctgtc agcccctgaa agactaggat
     8221 aatattccct gctctctgac aagacaaatt acctgtaatg agtgcagtag ctgaagggta
     8281 tacttttatt ttaaaatatg tcaataaccc cagtgactaa acgaatattg atttagcata
     8341 atgaagcctg agtaacgtga aaatgagctt tttcaagggg catggtaaag tctttctttt
     8401 tagctggttg taagaagctt ttgattcttt tcagccagct ggtaggaata tagaatttta
     8461 taagcaaacc atcaggaatg atagtgttgt ttctgataag caacatccaa atattttgac
     8521 cctgctttta gtggtttttt tcaaatctta ttttgagtct tacttttagt catagaatag
     8581 ctactgattt gatgcggtct ttaactgact taatattttt acaatttcaa tatattttgc
     8641 attggaatct ccagtaatga atattaaaat atatgtacaa tcatttgtag atgatatcaa
     8701 ttatattaag acatttcaga tgggctattg tagtatttaa tgtgccgtat tttatggtag
     8761 aataattctc agtctctgga catcaagatt gctttcagtg ggaatgaaga ttaatttact
     8821 tcagtcctga ttttttaggc atcaatgcat gttttcattt ttgtcagact tttaccctct
     8881 tttaatgtaa ttctcaactt cttatggatt tacttcccaa tacataaaat ccttcaaaac
     8941 aagaatgata ataattttta tactttttat aaaaataaat ttatttttag tccatcaagg
     9001 tgtctgaaga ttttatgcct aggtatctcc atatctaact tgataaggaa aataggataa
     9061 acaatgctgg taatagcagg aaagtaagta tttgaataag atgtcaaact gatatttcat
     9121 gtgaacctaa ctcattttat ggtaactaat aattatctta tttaaatcaa taggtaaaac
     9181 ctgtttagaa attaaaaatg agttacgatt taaagaaaat tcagatgact cattgtgagt
     9241 gctagttctc ttgtaggatg ccactggaaa tgttgaaatg aaaaatgtaa gtatatcttt
     9301 tggtggaaaa aaggatagtc tctaggacac aaaattactg ttttattttt ttctcaggag
     9361 tttgcctaag ggtgtgacag atgatctctg tcacttgtct tagttgtgtc ctgcaataaa
     9421 ctggatgctt tataaaatac tagacctgtg atttcgtatg ctgtaatatt tcatttctcc
     9481 atcacccctc caaattattt cttagtttgg agtaaaataa taaatgtatt atagtcaaca
     9541 tctcttgacc cctctttagt ttcagctaaa ctaagcatgt gtgtttgtgt gttcatttta
     9601 tagttcatgt gtagaactat gtgaattaaa tttaagaaac atgtaaagta gaggaaatag
     9661 ttttctggag aaatttttcc tttttggata ttatgccctt ttccattgct tttctctgct
     9721 tgaaagcaaa aaaaagtacc ctacccctgt tctcctttag ggaaaaacta ttcctataaa
     9781 gtatttttaa atcgtgcaag tcattgccta gggttagcta aaacatttct ttttaaaaag
     9841 gagaaaatgc cctggcttta acattttctt gtatttgtat ctattaagat aaacagttta
     9901 ctttgataca gtacatacca atctacttaa ttttttttcc aggattcctt ttactatgtt
     9961 tggtctgacc ttttatgata acttaatatg ggaacaaatt agcatataat tctattttcc
    10021 atgtgacctc aaccagttgc agaattgtac cactacttta gggggggcaa tttgacagtt
    10081 tatgtagact atagcattaa ttgttcccaa atgttcagtg catcctggct aatgtgttat
    10141 tgaaggtgtt ttcacgtaag cagttagagg aagcacttca cccctattac taagttatta
    10201 aaatgcctcc taaaggtagc attttaaatt agtatacata attgattagt aatttgtctt
    10261 ctcccaagca taaaacagca tagcagagtt aagtgtgacc agtgaagtat aagatattag
    10321 ggattgatgg tgacaatgat catagcaact aaatggattt tttttttctt ttagattcag
    10381 ccgttggtct ttgaaatttc ctgtgatgtg tttcaatcta gatgcaaaga acatggaaaa
    10441 atcaaagtgc tcgagtggtt taaatatgtt ttgggtattc ctgtttatag actataatac
    10501 ttttccaatt aaaatcctca gttgtcacgc agaagaaggt taagcactag tagaccacct
    10561 cccctgcgag ctaagctgga cagccaatga cgggtaagag agtgacattt ttcactaacc
    10621 taagacagga gggccgtcag agctactgcc taatccaaag acgggtaaaa gtgataaaaa
    10681 tgtatcactc caacctaaga caggcgcagc ttccgaggga tttgagatcc agacatgata
    10741 agatacattg atgagtttgg acaaaccaaa actagaatgc agtgaaaaaa atgccttatt
    10801 tgtgaaattt gtgatgctat tgccttattt gtaaccatta taagctgcaa taaacaagtt
    10861 tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat
    10921 aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc
    10981 attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca
    11041 cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc
    11101 gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat
    11161 ctttctaggg ttaagaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    11221 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    11281 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
    11341 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
    11401 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
    11461 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
    11521 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
    11581 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
    11641 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
    11701 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
    11761 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
    11821 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
    11881 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    11941 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    12001 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
    12061 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    12121 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    12181 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    12241 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    12301 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    12361 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    12421 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    12481 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    12541 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    12601 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    12661 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    12721 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    12781 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    12841 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    12901 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    12961 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    13021 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    13081 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    13141 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    13201 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    13261 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    13321 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    13381 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    13441 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    13501 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    13561 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    13621 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    13681 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    13741 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
    13801 gattacgcca aggtcgactt aaccctagaa agataatcat attgtgacgt acgttaaaga
    13861 taatcatgcg taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt
    13921 aatttgaata gatattaagt tttattatat ttacacttac atactaataa taaattcaac
    13981 aaacaattta tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct
    14041 ataaagtaac aaaactttta gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
    14101 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
    14161 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
    14221 tacaaatgtg gtatggctga ttatgatcct ctggagattt agccctccca cacataacca
    14281 gagggcagca attcacgaat cccaactgcc gtcggctgtc catcactgtc cttcactatg
    14341 gctttgatcc caggatgcag atcgagaagc acctgtcggc accgtccgca ggggctcaag
    14401 atgcccctgt tctcatttcc gatcgcgacg atacaagtca ggttgccagc tgccgcagca
    14461 gcagcagtgc ccagcaccac gagttctgca caaggtcccc cagtaaaatg atatacattg
    14521 acaccagtga agatgcggcc gtcgctagag agagctgcgc tggcgacgct gtagtcttca
    14581 gagatgggga tgctgttgat tgtagccgtt gctctttcaa tgagggtgga ttcttcttga
    14641 gacaaaggct tggccatggg gccggggttc tcctccacgt cgccggcctg cttcagcagg
    14701 ctgaagttgg tggcgccgct gcccccgggg agcatgtcaa ggtcaaaatc gtcaagagcg
    14761 tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat cggctgggag catgtctaag
    14821 tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc actttagctg tttctccagg
    14881 ccacatatga ttagttccag gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac
    14941 agctcaattg cttgtttcag aagtgggggc atagaatcgg tggtaggtgt ctctctttcc
    15001 tcttttgcta cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg
    15061 gcggacagag cgtacagtgc gttctccagg gagaagcctt gctgacacag gaacgcgagc
    15121 tgattttcca gggtttcgta ctgtttctct gttgggcggg tgccgagatg cactttagcc
    15181 ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg cgttgttccg cagaaagtct
    15241 tgccatgact cgccttccag ggggcaggag tgggtatgat gcctgtccag catctcgatt
    15301 ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt aggctgctca
    15361 actcccagct tttgagcgag tttccttgtc gtcaggcctt cgataccgac tccattgagt
    15421 aattccagag cagagtttat gactttgctc ttgtccagtc tagacatctt atcgtcatcg
    15481 tctttgtaat ccatggtggc ggatcccgcg tcacgacacc tgtgttctgg cggcaaaccc
    15541 gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc ccccaccctc
    15601 gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc gccaccttct
    15661 ctaggcaccg gttcaattgc cgacccctcc ccccaacttc tcggggactg tgggcgatgt
    15721 gcgctctgcc cactgacggg caccggagcc actcgagtgg aatt
//
