LOCUS       Exported               10554 bp ds-DNA     circular SYN 11-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 1, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             675..683
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    5516..5650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5971..5987)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6461..6565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6566..7426
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7597..8185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8473..8494
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8509..8539
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8547..8563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8571..8587
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8851..8932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(9049..9447)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(9448..9504)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(9514..10257)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(10258..10281)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      10281..10290
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(10310..10521)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccgccgcagc cgcagcccct ggcgctaacg gtcggtaacg
      421 gcccgcgcgc gccgcccgcc gggggctcgc gccagccacg agggagcgtc cgcggcccgc
      481 gcgcccgcgc ggcggaggag aggtgttaag tgtgatgctt ccataataca tttggatgct
      541 gtcagctaag ttcacttctg aactaagggg ttcctccaaa tgttggctga aattcatccc
      601 aaggctggtc tgcaaagtct gcaattcata atggagctac tgtactggct attggaagga
      661 ggagattctg aagataagga ggtaatatta tctcttttaa aagaatactt tcctctgtaa
      721 tcctgaatct ttattacatg taagaacttt gtgcagtaga cagcaatttc tttgaatttg
      781 gtatatggaa acaattttat tttcctctgc taagtttttg agcctgcctc ttctagtgcc
      841 atggactgca ttggtagagc tgagaaatat catttagcca tactcagcac ccttaaaata
      901 gcttctttct gagaattaga tctgtgaagg tgtcctgcac agttcttgta gatgtcattt
      961 tagtttgtgg ttgacgtgca tgcatttagc atgttgctta accgtcctca ttcgcctccc
     1021 agttctttgt tgccttcatt tggggggatg tgttttctgc tggatgattt actgctagac
     1081 atgaccaaac tctgagtata agacttggtg tttggcagct ggttccagtg ctctgctgag
     1141 aagtagttgg gcccagcctg gggcattgta gtgtcctgga ggccgggagc tcctgcacag
     1201 gggttctttt cctggttcag agccttaggt gcctttccac ttttcacgta atcttttcct
     1261 aggttggctt gctgcttact ttgcagctgt tgcagggatt cacatggaat ggagggctcc
     1321 cttttattgt ggattatttc tttgataatt tacccatgtg ctttttcatt ttcaaaaacc
     1381 cagtggtgtt taagaatgaa agattcttga agaacaaaaa ttgggttaaa tttgtatcta
     1441 tcagaaaaga ttctatcctc tgatgctatg taggcacatt gaatatgcag gctaaattaa
     1501 aaacagagta aaaccttttt ataatatgcc aattactata tctaagaatg tttatacagg
     1561 ctgaaatttt gtaatggtat ttatatctgt tttccttttt aaggaaaata atatactttt
     1621 ctaggcatca aaaatatctg cccctttcac taatgctgat gtaccacctt cccccaaccc
     1681 ccaacgctaa atttgactgg cttaaaaaca tctgccccct gaactatatc tggggaggaa
     1741 tattaataaa acaatgaagg acttcatggt gtctagttat ataattagga aattggttag
     1801 aaacgtggct aaaaccagtt tctttcatta aaagaactag tgtacattta aaaacaaaaa
     1861 ccttcaggaa aaagactctt tgatctttaa gtcaaatagt atttttggtt aacacagcag
     1921 gaaggggaaa tataccaatt tcagattctt ttatttatgc ctaagtagag gttgtaagca
     1981 gctaaggaag tgattaaaat gtagtctagt aaaaaatggt gctgattact tggaaagcag
     2041 tttacatttg caaaaaaatt cagtattatg accttcacca acttttacac atcatatact
     2101 tgggttatta cttatggtat agcttgtgta tggaatgcaa gggtattttc aaattgacta
     2161 ggtcttgctg gtcatcttaa aatatgttac aatattacaa aattgaaatg taatattttt
     2221 taatagaagg aaaatataaa tttaatatct gggcaattga gacctttaaa cttactttaa
     2281 aagtatgatc ttgatgtata tgatactgtt ttgtctttgc tatattaaca gaattagagg
     2341 ggtgttctgc aattcaaata ccttatatat tccaaatttt attctctata atggactttt
     2401 aaaataaaag gtatatgtgc ttcaagaggg caaaatttga atcatgagct aatttgctaa
     2461 gcatcagatt atagaaaagc atccttgatt aatttggaac tgtgaaaggg ggcgggtaaa
     2521 actgttttct gcagaaattt actagtgcag caaccattta aattaaatgt ttgttaacat
     2581 aatagtgatg gcattttctc ctccccctcc ttgtggtttt gtccaactag atgttacagt
     2641 ggcagttgca ctgactgtta agtgtttaaa tgatgacacc attatgtgaa gtgattttga
     2701 aatgagagat tccagccaag aattacatct gctcccatct ccttcaaatc atactctctg
     2761 gcagtacaga ttatgattga tttgtttgtg acagattgca ggaaacagtc attgattttt
     2821 caatatttta ccttaaaatt atttacagtt gtaaccatgg ggaggtattt tcatgggctg
     2881 tcagcccctg aaagactagg ataatattcc ctgctctctg acaagacaaa ttacctgtaa
     2941 tgagtgcagt agctgaaggg tatactttta ttttaaaata tgtcaataac cccagtgact
     3001 aaacgaatat tgatttagca taatgaagcc tgagtaacgt gaaaatgagc tttttcaagg
     3061 ggcatggtaa agtctttctt tttagctggt tgtaagaagc ttttgattct tttcagccag
     3121 ctggtaggaa tatagaattt tataagcaaa ccatcaggaa tgatagtgtt gtttctgata
     3181 agcaacatcc aaatattttg accctgcttt tagtggtttt tttcaaatct tattttgagt
     3241 cttactttta gtcatagaat agctactgat ttgatgcggt ctttaactga cttaatattt
     3301 ttacaatttc aatatatttt gcattggaat ctccagtaat gaatattaaa atatatgtac
     3361 aatcatttgt agatgatatc aattatatta agacatttca gatgggctat tgtagtattt
     3421 aatgtgccgt attttatggt agaataattc tcagtctctg gacatcaaga ttgctttcag
     3481 tgggaatgaa gattaattta cttcagtcct gattttttag gcatcaatgc atgttttcat
     3541 ttttgtcaga cttttaccct cttttaatgt aattctcaac ttcttatgga tttacttccc
     3601 aatacataaa atccttcaaa acaagaatga taataatttt tatacttttt ataaaaataa
     3661 atttattttt agtccatcaa ggtgtctgaa gattttatgc ctaggtatct ccatatctaa
     3721 cttgataagg aaaataggat aaacaatgct ggtaatagca ggaaagtaag tatttgaata
     3781 agatgtcaaa ctgatatttc atgtgaacct aactcatttt atggtaacta ataattatct
     3841 tatttaaatc aataggtaaa acctgtttag aaattaaaaa tgagttacga tttaaagaaa
     3901 attcagatga ctcattgtga gtgctagttc tcttgtagga tgccactgga aatgttgaaa
     3961 tgaaaaatgt aagtatatct tttggtggaa aaaaggatag tctctaggac acaaaattac
     4021 tgttttattt ttttctcagg agtttgccta agggtgtgac agatgatctc tgtcacttgt
     4081 cttagttgtg tcctgcaata aactggatgc tttataaaat actagacctg tgatttcgta
     4141 tgctgtaata tttcatttct ccatcacccc tccaaattat ttcttagttt ggagtaaaat
     4201 aataaatgta ttatagtcaa catctcttga cccctcttta gtttcagcta aactaagcat
     4261 gtgtgtttgt gtgttcattt tatagttcat gtgtagaact atgtgaatta aatttaagaa
     4321 acatgtaaag tagaggaaat agttttctgg agaaattttt cctttttgga tattatgccc
     4381 ttttccattg cttttctctg cttgaaagca aaaaaaagta ccctacccct gttctccttt
     4441 agggaaaaac tattcctata aagtattttt aaatcgtgca agtcattgcc tagggttagc
     4501 taaaacattt ctttttaaaa aggagaaaat gccctggctt taacattttc ttgtatttgt
     4561 atctattaag ataaacagtt tactttgata cagtacatac caatctactt aatttttttt
     4621 ccaggattcc ttttactatg tttggtctga ccttttatga taacttaata tgggaacaaa
     4681 ttagcatata attctatttt ccatgtgacc tcaaccagtt gcagaattgt accactactt
     4741 tagggggggc aatttgacag tttatgtaga ctatagcatt aattgttccc aaatgttcag
     4801 tgcatcctgg ctaatgtgtt attgaaggtg ttttcacgta agcagttaga ggaagcactt
     4861 cacccctatt actaagttat taaaatgcct cctaaaggta gcattttaaa ttagtataca
     4921 taattgatta gtaatttgtc ttctcccaag cataaaacag catagcagag ttaagtgtga
     4981 ccagtgaagt ataagatatt agggattgat ggtgacaatg atcatagcaa ctaaatggat
     5041 tttttttttc ttttagattc agccgttggt ctttgaaatt tcctgtgatg tgtttcaatc
     5101 tagatgcaaa gaacatggaa aaatcaaagt gctcgagtgg tttaaatatg ttttgggtat
     5161 tcctgtttat agactataat acttttccaa ttaaaatcct cagttgtcac gcagaagaag
     5221 gttaagctgt atttgattgc cagttttact gaaaatgctt agtattttac agtatcacca
     5281 aatatatttt gtttagccaa ggtataggaa aaataaaata aattgtatag gttgaactag
     5341 tagaccacct cccctgcgag ctaagctgga cagccaatga cgggtaagag agtgacattt
     5401 ttcactaacc taagacagga gggccgtcag agctactgcc taatccaaag acgggtaaaa
     5461 gtgataaaaa tgtatcactc caacctaaga caggcgcagc ttccgaggga tttgagatcc
     5521 agacatgata agatacattg atgagtttgg acaaaccaaa actagaatgc agtgaaaaaa
     5581 atgccttatt tgtgaaattt gtgatgctat tgccttattt gtaaccatta taagctgcaa
     5641 taaacaagtt tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
     5701 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
     5761 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
     5821 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
     5881 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
     5941 cgcagactat ctttctaggg ttaagaattc actggccgtc gttttacaac gtcgtgactg
     6001 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6061 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6121 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     6181 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     6241 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     6301 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     6361 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     6421 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6481 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6541 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6601 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6661 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6721 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6781 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6841 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6901 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6961 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7021 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7081 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7141 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7201 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7261 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7321 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7381 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7441 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7501 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7561 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7621 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7681 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7741 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7801 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7861 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7921 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7981 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8041 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8101 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8161 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8221 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8281 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8341 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     8401 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     8461 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     8521 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     8581 ctatgaccat gattacgcca aggtcgactt aaccctagaa agataatcat attgtgacgt
     8641 acgttaaaga taatcatgcg taaaattgac gcatgtgttt tatcggtctg tatatcgagg
     8701 tttatttatt aatttgaata gatattaagt tttattatat ttacacttac atactaataa
     8761 taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaac aaaaactcaa
     8821 aatttcttct ataaagtaac aaaactttta gcagtgaaaa aaatgcttta tttgtgaaat
     8881 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     8941 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     9001 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctggagattt agccctccca
     9061 cacataacca gagggcagca attcacgaat cccaactgcc gtcggctgtc catcactgtc
     9121 cttcactatg gctttgatcc caggatgcag atcgagaagc acctgtcggc accgtccgca
     9181 ggggctcaag atgcccctgt tctcatttcc gatcgcgacg atacaagtca ggttgccagc
     9241 tgccgcagca gcagcagtgc ccagcaccac gagttctgca caaggtcccc cagtaaaatg
     9301 atatacattg acaccagtga agatgcggcc gtcgctagag agagctgcgc tggcgacgct
     9361 gtagtcttca gagatgggga tgctgttgat tgtagccgtt gctctttcaa tgagggtgga
     9421 ttcttcttga gacaaaggct tggccatggg gccggggttc tcctccacgt cgccggcctg
     9481 cttcagcagg ctgaagttgg tggcgccgct gcccccgggg agcatgtcaa ggtcaaaatc
     9541 gtcaagagcg tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat cggctgggag
     9601 catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc actttagctg
     9661 tttctccagg ccacatatga ttagttccag gccgaaaagg aaggcaggtt cggctccctg
     9721 ccggtcgaac agctcaattg cttgtttcag aagtgggggc atagaatcgg tggtaggtgt
     9781 ctctctttcc tcttttgcta cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa
     9841 gtggcccacg gcggacagag cgtacagtgc gttctccagg gagaagcctt gctgacacag
     9901 gaacgcgagc tgattttcca gggtttcgta ctgtttctct gttgggcggg tgccgagatg
     9961 cactttagcc ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg cgttgttccg
    10021 cagaaagtct tgccatgact cgccttccag ggggcaggag tgggtatgat gcctgtccag
    10081 catctcgatt ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt
    10141 aggctgctca actcccagct tttgagcgag tttccttgtc gtcaggcctt cgataccgac
    10201 tccattgagt aattccagag cagagtttat gactttgctc ttgtccagtc tagacatctt
    10261 atcgtcatcg tctttgtaat ccatggtggc ggatcccgcg tcacgacacc tgtgttctgg
    10321 cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc
    10381 ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc
    10441 gccaccttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc tcggggactg
    10501 tgggcgatgt gcgctctgcc cactgacggg caccggagcc actcgagtgg aatt
//
