LOCUS       pGMF2-D                 3526 bp    DNA     circular SYN 09-JUL-2025
DEFINITION  Level 1 AtU6-26p::sgRNA2.
ACCESSION   VB250106-1631upb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Misc._feature   7..157
                     /note="LB"
                     /note="color: #a6acb3; direction: LEFT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /modified_by="Florian"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Final L2 for targeting PDS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB (<a
                     href=""urn:local:.:7o5-d1z2kwz"">Final L2 for targeting
                     PDS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:ix-eboo9ps"">pICSL4723 L2 backbone
                     - pICSL11059 35Sp::HPTII:35St - pFH52 2x35Sp::SpCas9:NOSt
                     - pGMF001 with PDS target1 - pGMF002 with PDS
                     target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:1d4-dvu4edz"">pICSL4723 L2 backbone
                     - pGMF006 - pFH52 2x35Sp::SpCas9:NOSt - pGMF001 with PDS
                     target1 - pGMF002 with PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:7jv-d1yz8b0"">pGMF003 </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-1/-4; LB
                     (<a
                     href=""urn:local:.:n-hwkaxtn"">pGMF001a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-1/-4; LB
                     (<a href=""urn:local:.:7jj-d1yz4wq"">pGMF001</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-1/-4; LB
                     (<a href=""urn:local:.:7jr-d1yz7zq"">pGMF005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-1/-4; LB
                     (<a href=""urn:local:.:y-i5qen40"">pGMF001a</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-1/-4; LB
                     (<a href=""urn:local:.:7jn-d1yz5cw"">pGMF002</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-1/-4; LB
                     (<a href=""urn:local:.:7jz-d1yz8pq"">pGMF004</a>)"
                     /label="LB"
     primer_bind     72..95
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="1214696047758000ec8eea16-8136-4094-817c
                     -6424d1642ec0"
                     /created_by="primer3"
                     /Sequence="CTGGTGGCAGGATATATTGTGGTG"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pGMF2-D"
                     /label="GMF002"
     promoter        209..657
                     /SerialCloner_Color="&hFF7F00"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /modified_by="hahnf"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGMF001a_with_Arabidopsis_U6-26_promote
                     r"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AtU6-26p
                     (<a
                     href=""urn:local:.:n-hwkaxtn"">pGMF001a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AtU6-26p (<a
                     href=""urn:local:.:j-hwkax9k"">pGMF002a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/-1;
                     AtU6-26 (<a href=""urn:local:.:y-i5qen40"">pGMF001a</a>)"
                     /label="AtU6-26p"
     CDS             830..1153
                     /codon_start=1
                     /gene="lacZ-alpha"
                     /product="beta-galactosidase"
                     /note="alpha fragment of lacZ from pUC19 cloning 
                     vector.Used for alpha complementation alongside omega
                     fragment (usually in host)"
                     /note="Derived using Geneious Prime 2020.1.2
                     'AnnotatefromDatabase' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.0.3
                     'AnnotatefromDatabase' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0;lacZ-alpha (L1_target1 in pTwist
                     Amp High Copy)"
                     /note="/Color=#FF00FF"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     lacZ-alpha(L1_target2 in pTwist Amp High Copy)"
                     /note="/Transferred_From=L1_target2 in pTwist Amp High 
                     Copy"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Alternative_Match=93.79%; 
                     Adjusted-Interval-0:54/-93;lacZa"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     lacZ-alpha"
                     /note="/X-Transferred_From=lacZ-alpha"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/kind=reporter"
                     /Transferred_Translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQR
                     RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCG
                     ISHRIWCTLSTICSDAA"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGMF001a_with_Arabidopsis_U6-26_promote
                     r"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZ-alpha (<a
                     href=""urn:local:.:n-hwkaxtn"">pGMF001a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZ-alpha (<a
                     href=""urn:local:.:j-hwkax9k"">pGMF002a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (<a href=""urn:local:.:7jj-d1yz4wq"">pGMF001</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (<a href=""urn:local:.:y-i5qen40"">pGMF001a</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lacZα (<a href=""urn:local:.:7jn-d1yz5cw"">pGMF002</a>)"
                     /label="lacZ-alpha"
     misc_RNA        1250..1335
                     /created_by="Florian"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGMF001a_with_Arabidopsis_U6-26_promote
                     r"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; improved
                     sgRNA backbone (Dang et al., 2015) (<a
                     href=""urn:local:.:n-hwkaxtn"">pGMF001a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     improved sgRNA backbone (Dang et al., 2015) (<a
                     href=""urn:local:.:7jj-d1yz4wq"">pGMF001</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     improved sgRNA backbone (Dang et al., 2015) (<a
                     href=""urn:local:.:j-hwkax9k"">pGMF002a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     improved sgRNA backbone (Dang et al., 2015) (<a
                     href=""urn:local:.:ix-eboo9ps"">pICSL4723 L2 backbone -
                     pICSL11059 35Sp::HPTII:35St - pFH52 2x35Sp::SpCas9:NOSt -
                     pGMF001 with PDS target1 - pGMF002 with PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     improved sgRNA backbone (Dang et al., 2015) (<a
                     href=""urn:local:.:ix-eboo9ps"">pICSL4723 L2 backbone -
                     pICSL11059 35Sp::HPTII:35St - pFH52 2x35Sp::SpCas9:NOSt -
                     pGMF001 with PDS target1 - pGMF002 with PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     improved sgRNA backbone (Dang et al., 2015) (<a
                     href=""urn:local:.:1d4-dvu4edz"">pICSL4723 L2 backbone -
                     pGMF006 - pFH52 2x35Sp::SpCas9:NOSt - pGMF001 with PDS
                     target1 - pGMF002 with PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     improved sgRNA backbone (Dang et al., 2015) (<a
                     href=""urn:local:.:1d4-dvu4edz"">pICSL4723 L2 backbone -
                     pGMF006 - pFH52 2x35Sp::SpCas9:NOSt - pGMF001 with PDS
                     target1 - pGMF002 with PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     improved sgRNA backbone (Dang et al., 2015) (<a
                     href=""urn:local:.:y-i5qen40"">pGMF001a</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     improved sgRNA backbone (Dang et al., 2015) (<a
                     href=""urn:local:.:7jn-d1yz5cw"">pGMF002</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     improved sgRNA backbone (Dang et al., 2015) (<a
                     href=""urn:local:.:1h-ib5wyr0"">pGMF001 with integrated
                     annealed primers</a>)"
                     /label="improved sgRNA backbone (Dang et al., 2015)"
     terminator      1336..1344
                     /created_by="Florian"
                     /label="PolyT"
     Misc._feature   complement(1376..1503)
                     /created_by="Florian"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGMF001a_with_Arabidopsis_U6-26_promote
                     r"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RB short
                     (<a
                     href=""urn:local:.:n-hwkaxtn"">pGMF001a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:7jj-d1yz4wq"">pGMF001</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:7jr-d1yz7zq"">pGMF005</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:y-i5qen40"">pGMF001a</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:7jn-d1yz5cw"">pGMF002</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:7jz-d1yz8pq"">pGMF004</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:1h-ib5wyr0"">pGMF001 with
                     integrated annealed primers</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-6/-4; RB
                     (<a href=""urn:local:.:7o5-d1z2kwz"">Final L2 for
                     targeting PDS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-6/-4; RB
                     (<a href=""urn:local:.:ix-eboo9ps"">pICSL4723 L2 backbone
                     - pICSL11059 35Sp::HPTII:35St - pFH52 2x35Sp::SpCas9:NOSt
                     - pGMF001 with PDS target1 - pGMF002 with PDS
                     target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-6/-4; RB
                     (<a href=""urn:local:.:1d4-dvu4edz"">pICSL4723 L2 backbone
                     - pGMF006 - pFH52 2x35Sp::SpCas9:NOSt - pGMF001 with PDS
                     target1 - pGMF002 with PDS target2</a>)"
                     /label="RB short"
     primer_bind     complement(1490..1515)
                     /%GC="53.8"
                     /Hairpin_Tm="54.9"
                     /Self_Dimer_Tm="21.4"
                     /Tm="66.5"
                     /annotation_group="12146958772118002072b6d9-652c-4f25-950d
                     -989cc5c21d43"
                     /created_by="primer3"
                     /Sequence="GAACCCTGTGGTTGGCATGCACATAC"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pGMF2-D"
                     /label="GMF001"
     Rep_origin      complement(1707..2295)
                     /Application_Notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /Full_Name="pUC origin of replication"
                     /References="Mol Microbiol. 6:3385 (1992)"
                     /Subtype="E. coli replication_origin"
                     /__SeqFeature__="True"
                     /__level__=0
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /name="pUC ori"
                     /note="color: #0c5d90; direction: LEFT"
                     /official_designation="pUC ori"
                     /uuid="f09c4dacdc56460eac9fa1805e592776"
                     /label="pUC ori"
     CDS             complement(2466..3326)
                     /Application_Notes="Allows E. coli to be resistant to
                     ampicillin."
                     /Full_Name="Ampicillin resistance gene"
                     /References="Proc Natl Acad Sci U S A. 75: 3737 (1978)"
                     /Selection_Drug="Ampicillin"
                     /Subtype="E. coli selection marker"
                     /Usage="Positive selection"
                     /__SeqFeature__="True"
                     /__level__=0
                     /application_notes="Allows E. coli to be resistant to
                     ampicillin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /name="Ampicillin"
                     /note="color: #f58600; direction: LEFT"
                     /official_designation="Ampicillin"
                     /uuid="39ee7c4ce93d4075b0df0df5df6b9861"
                     /label="Ampicillin"
     promoter        complement(3327..3431)
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTwist Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:17-c2in4sl"">pTwist Amp
                     High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:1z-ce2zb9d"">pBRIDT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; amp
                     (<a href=""urn:local:.:52-c2iy3lf"">L1_target1 in pTwist
                     Amp High Copy - fake primer PDS target1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; amp
                     (<a href=""urn:local:.:5d-c2iy4z2"">L1_target2 in pTwist
                     Amp High Copy - fake primer PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; amp
                     (<a href=""urn:local:.:1b-c2incxi"">L1_target1 in pTwist
                     Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; amp
                     (<a href=""urn:local:.:1f-c2incxl"">L1_target2 in pTwist
                     Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; amp
                     promoter (<a href=""urn:local:.:y-i5qen40"">pGMF001a</a>)"
                     /Alternative_Match="99.05%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:7n4-d1z1usp"">pGMF001 with
                     PDS target1</a>)"
                     /Alternative_Match="99.05%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:av-i9anmpc"">pGMF002 with
                     Arabidopsis PDS target2</a>)"
                     /Alternative_Match="99.05%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:j-hwkax9k"">pGMF002a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /label="AmpR promoter"
ORIGIN      
        1 tctgactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
       61 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
      121 taataacaca ttgcggacgt ttttaatgta ctggggtgga tgcagtgggc cccactctgt
      181 gaagacaatt acgaattccc atggggagaa gcttcgttga acaacggaaa ctcgacttgc
      241 cttccgcaca atacatcatt tcttcttagc tttttttctt cttcttcgtt catacagttt
      301 ttttttgttt atcagcttac attttcttga accgtagctt tcgttttctt ctttttaact
      361 ttccattcgg agtttttgta tcttgtttca tagtttgtcc caggattaga atgattaggc
      421 atcgaacctt caagaatttg attgaataaa acatcttcat tcttaagata tgaagataat
      481 cttcaaaagg cccctgggaa tctgaaagaa gagaagcagg cccatttata tgggaaagaa
      541 caatagtatt tcttatatag gcccatttaa gttgaaaaca atcttcaaaa gtcccacatc
      601 gcttagataa gaaaacgaag ctgagtttat atacagctag agtcgaagta gtgattgtga
      661 gaccgcagct ggcacgacag gtttgccgac tggaaagcgg gcagtgagcg caacgcaatt
      721 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      781 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      841 tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
      901 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      961 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     1021 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     1081 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     1141 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     1201 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acggtctcag tttcagagct
     1261 atgctggaaa cagcatagca agttgaaata aggctagtcc gttatcaact tgaaaaagtg
     1321 gcaccgagtc ggtgcttttt ttttcgctgg gattgtcttc tgcacgaagt ggtttaaact
     1381 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     1441 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     1501 ccaaccacag ggttccccag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1561 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1621 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1681 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1741 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1801 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1861 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1921 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1981 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2041 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2101 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2161 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2221 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2281 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2341 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2401 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2461 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2521 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2581 ggccccagtg ctgcaatgat accgcgagat ccacgctcac cggctccaga tttatcagca
     2641 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2701 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2761 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2821 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2881 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2941 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3001 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3061 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3121 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3181 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3241 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3301 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3361 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3421 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     3481 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
