LOCUS       pGMF3                  12803 bp ds-DNA     circular     09-JUL-2025
DEFINITION  Level2 backbone based on pICSL4723.
ACCESSION
VERSION     .
KEYWORDS    pAGM4723- OD fixed[1].
SOURCE      natural DNA sequence
  ORGANISM  unspecified.
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 16 May 2016 from SnapGene Viewer 2.8.1
            http://www.snapgene.com
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     promoter        34..243
                     /note="propant"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="propant"
     misc_feature    262..1191
                     /note="crtB"
                     /note="color: #a6acb3"
                     /ApEinfo_label="crtB"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /modified_by="Florian"
                     /label="crtB"
     misc_feature    1214..2692
                     /note="crtI"
                     /note="color: #a6acb3"
                     /ApEinfo_label="crtI"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /modified_by="Florian"
                     /label="crtI"
     misc_feature    2715..3863
                     /note="crtY"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="crtY"
     misc_feature    3886..5745
                     /note="dxs E.coli"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="dxs E.coli"
     misc_feature    5771..6496
                     /note="crtW aa"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="crtW aa"
     misc_feature    6522..7070
                     /note="idi E.coli"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="idi E.coli"
     misc_feature    7093..7998
                     /note="crtE"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="crtE"
     terminator      8022..8086
                     /note="kdgm ter"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="kdgm ter"
     Misc._feature   complement(8123..8248)
                     /note="RB"
                     /note="color: #a6acb3; direction: LEFT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /modified_by="Florian"
                     /label="RB"
     primer_bind     complement(8241..8266)
                     /%GC="53.8"
                     /Hairpin_Tm="54.9"
                     /Self_Dimer_Tm="21.4"
                     /Tm="66.5"
                     /annotation_group="1214696642436900ba2277bb-5670-4f56-97d0
                     -ed58d965bfc5"
                     /created_by="primer3"
                     /Sequence="GAACCCTGTGGTTGGCATGCACATAC"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pGMF3"
                     /label="GMF001"
     CDS             11032..11823
                     /codon_start=1
                     /note="NPTII"
                     /note="color: #993366"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIA FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NPTII"
     rep_origin      11986..12600
                     /note="rep - pMB1"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /modified_by="Florian"
                     /label="rep - pMB1"
     Misc._feature   12652..12802
                     /note="LB"
                     /note="color: #a6acb3; direction: LEFT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /modified_by="Florian"
                     /label="LB"
     primer_bind     12717..12740
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="12146967239314005dff9728-7493-468c-a8ad
                     -ae573ac8be03"
                     /created_by="primer3"
                     /Sequence="CTGGTGGCAGGATATATTGTGGTG"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pGMF3"
                     /label="GMF002"
ORIGIN      
        1 gaacactctg tgccttgtct tccagtggta tggggtaccg cacggtctgc caatccgacg
       61 gaggtttatg aattttccac cttttccaca agctcaacta gtattaacga tgtggattta
      121 gcaaaaaaaa cctgtaaccc taaatgtaaa ataacgggta agcctgccaa ccatgttatg
      181 gcagattaag cgtctttttg aagggcaccg catctttcgc gttgccgtaa atgtatccgt
      241 ttagcaataa ggaaattctt aatgaataat ccgtcgttac tcaatcatgc ggtcgaaacg
      301 atggcagttg gctcgaaaag ttttgcgaca gcctcaaagt tatttgatgc aaaaacccgg
      361 cgcagcgtac tgatgctcta cgcctggtgc cgccattgtg acgatgttat tgacgatcag
      421 acgctgggct ttcaggcccg gcagcctgcc ttacaaacgc ccgaacaacg tctgatgcaa
      481 cttgagatga aaacgcgcca ggcctatgca ggatcgcaga tgcacgaacc ggcgtttgcg
      541 gcttttcggg aagtggctat ggctcatgat atcgccccgg cttacgcgtt tgatcatctg
      601 gaaggcttcg ccatggatgt acgcgaagcg caatacagcc aactggatga tacgctgcgc
      661 tattgctatc acgttgcagg cgttgtcggc ttgatgatgg cgcaaatcat gggcgtgcgg
      721 gataacgcca cgctggaccg cgcctgtgac cttgggctgg catttcagtt gaccaatatt
      781 gctcgcgata ttgtggacga tgcgcatgcg ggccgctgtt atctgccggc aagctggctg
      841 gagcatgaag gtctgaacaa agagaattat gcggcacctg aaaaccgtca ggcgctgagc
      901 cgtatcgccc gtcgtttggt gcaggaagca gaaccttact atttgtctgc cacagccggc
      961 ctggcagggt tgcccctgcg ttccgcctgg gcaatcgcta cggcgaagca ggtttaccgg
     1021 aaaataggtg tcaaagttga acaggccggt cagcaagcct gggatcagcg gcagtcaacg
     1081 accacgcccg aaaaattaac gctgctgctg gccgcctctg gtcaggccct tacttcccgg
     1141 atgcgggctc atcctccccg ccctgcgcat ctctggcagc gcccgctcta ggcttactat
     1201 aaggatgttt gcaatgaaac caactacggt aattggtgca ggcttcggtg gcctggcact
     1261 ggcaattcgt ctacaagctg cggggatccc cgtcttactg cttgaacaac gtgataaacc
     1321 cggcggtcgg gcttatgtct acgaggatca ggggtttacc tttgatgcag gcccgacggt
     1381 tatcaccgat cccagtgcca ttgaagaact gtttgcactg gcaggaaaac agttaaaaga
     1441 gtatgtcgaa ctgctgccgg ttacgccgtt ttaccgcctg tgttgggagt cagggaaggt
     1501 ctttaattac gataacgatc aaacccggct cgaagcgcag attcagcagt ttaatccccg
     1561 cgatgtcgaa ggttatcgtc agtttctgga ctattcacgc gcggtgttta aagaaggcta
     1621 tctaaagctc ggtactgtcc cttttttatc gttcagagac atgcttcgcg ccgcacctca
     1681 actggcgaaa ctgcaggcat ggagaagcgt ttacagtaag gttgccagtt acatcgaaga
     1741 tgaacatctg cgccaggcgt tttctttcca ctcgctgttg gtgggcggca atcccttcgc
     1801 cacctcatcc atttatacgt tgatacacgc gctggagcgt gagtggggcg tctggtttcc
     1861 gcgtggcggc accggcgcat tagttcaggg gatgataaag ctgtttcagg atctgggtgg
     1921 cgaagtcgtg ttaaacgcca gagtcagcca tatggaaacg acaggaaaca agattgaagc
     1981 cgtgcattta gaggacggtc gcaggttcct gacgcaagcc gtcgcgtcaa atgcagatgt
     2041 ggttcatacc tatcgcgacc tgttaagcca gcaccctgcc gcggttaagc agtccaacaa
     2101 actgcagact aagcgcatga gtaactctct gtttgtgctc tattttggtt tgaatcacca
     2161 tcatgatcag ctcgcgcatc acacggtttg tttcggcccg cgttaccgcg agctgattga
     2221 cgaaattttt aatcatgatg gcctcgcaga ggacttctca ctttatctgc acgcgccctg
     2281 tgtcacggat tcgtcactgg cgcctgaagg ttgcggcagt tactatgtgt tggcgccggt
     2341 gccgcattta ggcaccgcga acctcgactg gacggttgag gggccaaaac tacgcgaccg
     2401 tatttttgcg taccttgagc agcattacat gcctggctta cggagtcagc tggtcacgca
     2461 ccggatgttt acgccgtttg attttcgcga ccagcttaat gcctatcatg gctcagcctt
     2521 ttctgtggag cccgttctta cccagagcgc ctggtttcgg ccgcataacc gcgataaaac
     2581 cattactaat ctctacctgg tcggcgcagg cacgcatccc ggcgcaggca ttcctggcgt
     2641 catcggctcg gcaaaagcga cagcaggttt gatgctggag gatctgatat gagcttttac
     2701 ccaggagaca gagaatgcaa ccgcattatg atctgattct cgtgggggct ggactcgcga
     2761 atggccttat cgccctgcga cttcagcagc agcaacctga tatgcgtatt ttgcttatcg
     2821 acgccgcacc ccaggcgggc gggaatcata cgtggtcatt tcaccacgat gatttgactg
     2881 agagccaaca tcgttggata gctccgctgg tggttcatca ctggcccgac tatcaggtac
     2941 gctttcccac acgccgtcgt aagctgaaca gcggctactt ttgtattact tctcagcgtt
     3001 tcgctgaggt tttacagcga cagtttggcc cgcacttgtg gatggatacc gcggtcgcag
     3061 aggttaatgc ggaatctgtt cggttgaaaa agggtcaggt tatcggtgcc cgcgcggtga
     3121 ttgacgggcg gggttatgcg gcaaattcag cactgagcgt gggcttccag gcgtttattg
     3181 gccaggaatg gcgattgagt cacccgcatg gtttatcatc tccgattatc atggatgcca
     3241 cggtcgatca gcaaaatggt tatcgcttcg tgtacagcct gccgctctcg ccgaccagat
     3301 tgttaattga agatacgcac tatattgata atgcgacatt agatcctgaa tgcgcgcggc
     3361 aaaatatttg cgactatgcc gcgcaacagg gttggcagct tcagacactg ctgcgagaag
     3421 aacagggcgc cttacccatt actctgtcgg gcaatgccga cgcattctgg cagcagcgcc
     3481 ccctggcctg tagtggatta cgtgccggtc tgttccatcc taccaccggc tattcactgc
     3541 cgctggcggt tgccgtggcc gaccgcctga gtgcacttga tgtctttacg tcggcctcaa
     3601 ttcaccatgc cattacgcat tttgcccgcg agcgctggca gcagcagggc tttttccgca
     3661 tgctgaatcg catgctgttt ttagccggac ccgccgattc acgctggcgg gttatgcagc
     3721 gtttttatgg tttacctgaa gatttaattg cccgttttta tgcgggaaaa ctcacgctga
     3781 ccgatcggct acgtattctg agcggcaagc cgcctgttcc ggtattagca gcattgcaag
     3841 ccattatgac gactcatcgt taagcttcag aggaggaatt aagtaatgag ttttgatatt
     3901 gccaaatacc cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa
     3961 gagagtttac cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt
     4021 tccagcgggc acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat
     4081 gtctacaaca ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc ttatccgcat
     4141 aaaattttga ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggtctgcac
     4201 ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc
     4261 tccattagtg ccggaattgg tattgcggtt gctgccgaaa aagaaggcaa aaatcgccgc
     4321 accgtctgtg tcattggcga tggcgcgatt accgcaggca tggcgtttga agcgatgaat
     4381 cacgcgggcg atatccgtcc tgatatgctg gtgattctca acgacaatga aatgtcgatt
     4441 tccgaaaatg tcggcgcgct caacaaccat ctggcacagc tgctttccgg taagctttac
     4501 tcttcactgc gcgaaggcgg gaaaaaagtt ttctctggcg tgccgccaat taaagagctg
     4561 ctcaaacgca ccgaagaaca tattaaaggc atggtagtgc ctggcacgtt gtttgaggag
     4621 ctgggcttta actacatcgg cccggtggac ggtcatgatg tgctggggct tatcaccacg
     4681 ctaaagaaca tgcgcgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt
     4741 cgtggttatg aaccggcaga aaaagacccg atcactttcc atgccgtgcc taaatttgat
     4801 ccctccagcg gttgtttgcc gaaaagtagc ggcggtttgc cgagctattc aaaaatcttt
     4861 ggcgactggt tgtgcgaaac ggcagcgaaa gacaacaagc tgatggcgat tactccggcg
     4921 atgcgtgaag gttccggcat ggtcgagttt tcacgtaaat tcccggatcg ctacttcgac
     4981 gtggcaattg ccgagcaaca tgcggtgacc tttgctgcgg gtctggcgat tggtgggtac
     5041 aaacccattg tcgcgattta ctccactttc ctgcaacgcg cctatgatca ggtgctgcat
     5101 gacgtggcga ttcaaaagct tccggtcctg ttcgccatcg accgcgcggg cattgttggt
     5161 gctgacggtc aaacccatca gggtgctttt gatctctctt acctgcgctg cataccggaa
     5221 atggtcatta tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta taccggctat
     5281 cactataacg atggcccgtc agcggtgcgc tacccgcgtg gcaacgcggt cggcgtggaa
     5341 ctgacgccgc tggaaaaact accaattggc aaaggcattg tgaagcgtcg tggcgagaaa
     5401 ctggcgatcc ttaactttgg tacgctgatg ccagaagcgg cgaaagtcgc cgaatcgctg
     5461 aacgccacgc tggtcgatat gcgttttgtg aaaccgcttg atgaagcgtt aattctggaa
     5521 atggccgcca gccatgaagc gctggtcacc gtagaagaaa acgccattat gggcggcgca
     5581 ggcagcggcg tgaacgaagt gctgatggcc catcgtaaac cagtacccgt gctgaacatt
     5641 ggcctgccgg acttctttat tccgcaagga actcaggaag aaatgcgcgc cgaactcggc
     5701 ctcgatgccg ctggtatgga agccaaaatc aaggcctggc tggcataagc tttgtgtgag
     5761 gagatgtcga atgagcgcac atgccctgcc caaggcagat ctgaccgcca ccagcctgat
     5821 cgtatcgggc ggcatcatcg ccgcttggct ggccctgcat gtgcatgcgc tgtggtttct
     5881 ggacgcagcg gcgcatccca tcctggcgat cgcaaatttc ctggggctga cctggctgtc
     5941 ggtcggattg ttcatcatcg cgcatgacgc gatgcacggg tcggtggtgc cggggcgtcc
     6001 gcgcgccaat gcggcgatgg gccagcttgt cctgtggctg tatgccggat tttcgtggcg
     6061 caagatgatc gtcaagcaca tggcccatca ccgccatgcc ggaaccgacg acgaccccga
     6121 tttcgaccat ggcggcccgg tccgctggta cgcccgcttc atcggcacct atttcggctg
     6181 gcgcgagggg ctgcttctgc ccgtcatcgt gacggtctat gcgctgatcc ttggggatcg
     6241 ctggatgtac gtggtattct ggccgctgcc gtcgatcctg gcgtcgatcc agctgttcgt
     6301 gttcggcacc tggctgccgc accgccccgg ccacgacgcg ttcccggacc gccacaatgc
     6361 gcggtcgtcg cggatcagcg accccgtgtc gctgctgacc tgttttcact ttggcggtta
     6421 tcatcacgaa caccacctgc acccgacggt gccgtggtgg cgcctgccca gcacccgcac
     6481 caagggcgac accgcataag cttgagctga ggagattttg aatgcaaacg gaacacgtca
     6541 ttttattgaa tgcacaggga gttcccacgg gtacgctgga aaagtatgcc gcacacacgg
     6601 cagacacccg cttacatctc gcgttctcca gttggctgtt taatgccaaa ggacaattat
     6661 tagttacccg ccgcgcactg agcaaaaaag catggcctgg cgtgtggact aactcggttt
     6721 gtgggcaccc acaactggga gaaagcaacg aggacgcagt gatccgccgt tgccgttatg
     6781 agcttggcgt ggaaattacg cctcctgaat ctatctatcc tgactttcgc taccgcgcca
     6841 ccgatccgag tggcattgtg gaaaatgaag tgtgtccggt atttgccgca cgcaccacta
     6901 gtgcgttaca gatcaatgat gatgaagtga tggattatca atggtgtgat ttagcagatg
     6961 tattacacgg tattgatgca acgccgtggg cgttcagtcc gtggatggtg atgcaggcga
     7021 caaatcgcga agccagaaaa cgattatctg catttaccca gcttaaataa gctttgcctc
     7081 aggatttttg aaatgacggt ctgcgcaaaa aaacacgttc atctcactcg cgatgctgcg
     7141 gagcagttac tggctgatat tgatcgacgc cttgatcagt tattgcccgt ggagggagaa
     7201 cgggatgttg tgggtgccgc gatgcgtgaa ggtgcgctgg caccgggaaa acgtattcgc
     7261 cccatgttgc tgttgctgac cgcccgcgat ctgggttgcg ctgtcagcca tgacggatta
     7321 ctggatttgg cctgtgcggt ggaaatggtc cacgcggctt cgctgatcct tgacgatatg
     7381 ccctgcatgg acgatgcgaa gctgcggcgc ggacgcccta ccattcattc tcattacgga
     7441 gagcatgtgg caatactggc ggcggttgcc ttgctgagta aagcctttgg cgtaattgcc
     7501 gatgcagatg gcctcacgcc gctggcaaaa aatcgggcgg tttctgaact gtcaaacgcc
     7561 atcggcatgc aaggattggt tcagggtcag ttcaaggatc tgtctgaagg ggataagccg
     7621 cgcagcgctg aagctatttt gatgacgaat cactttaaaa ccagcacgct gttttgtgcc
     7681 tcgatgcaga tggcctcgat tgttgcgaat gcctccagcg aagcgcgtga ttgcctgcat
     7741 cgtttttcac ttgatcttgg tcaggcattt caactgctgg acgatttgac cgatggcatg
     7801 accgacaccg gtaaggatag caatcaggac gccggtaaat cgacgctggt caatctgtta
     7861 ggcccgaggg cggttgaaga acgtctgaga caacatcttc agcttgccag tgagcatctc
     7921 tctgcggcct gccaacacgg gcacgccact caacatttta ttcaggcctg gtttgacaaa
     7981 aaactcgctg ccgtcagtta agcttgcaag aattcaagct tggagtacgt taattagttg
     8041 tctgttttaa gccccgtgct ctccgccggg gcttttttat taaaaaccac tcaagtggaa
     8101 gacaagggac acgaagtgat ccgtttaaac tatcagtgtt tgacaggata tattggcggg
     8161 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     8221 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggagtcag
     8281 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     8341 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     8401 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     8461 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     8521 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     8581 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     8641 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     8701 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     8761 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     8821 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     8881 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     8941 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     9001 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     9061 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     9121 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     9181 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     9241 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     9301 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     9361 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     9421 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     9481 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt
     9541 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     9601 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     9661 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     9721 gcgtcgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     9781 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     9841 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     9901 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     9961 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
    10021 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
    10081 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
    10141 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
    10201 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
    10261 gagctagctg attggatgta ccgcgagatt acagaaggca agaacccgga cgtgctgacg
    10321 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
    10381 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
    10441 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
    10501 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
    10561 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
    10621 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaaa gcttctttcc tgtggatagc
    10681 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
    10741 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
    10801 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
    10861 gcataactgt ctggccagcg cacagccgaa cagctgcaaa aagcgcccac atcaaggctc
    10921 cgagtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    10981 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatggctaaa
    11041 atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg
    11101 gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta
    11161 aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg
    11221 atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat
    11281 ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa
    11341 gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac
    11401 tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg
    11461 gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga agaggacact
    11521 ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa
    11581 cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta
    11641 agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc
    11701 tgcgtccggt cgctcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac
    11761 ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg
    11821 ttttagctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    11881 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    11941 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    12001 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    12061 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    12121 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    12181 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    12241 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    12301 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    12361 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    12421 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    12481 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    12541 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    12601 gcctttttac ggttcctgct cggatctgtt ggaccggaca gtagtcatgg ttgatgggct
    12661 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
    12721 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
    12781 gacgttttta atgtactggg gtt
//
