LOCUS       pGMF6                   5780 bp ds-DNA     circular SYN 09-JUL-2025
DEFINITION  Level1 35Sp::eGFP:35St.
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 20, 2021 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     Misc._feature   517..667
                     /note="LB"
                     /note="color: #a6acb3; direction: LEFT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /modified_by="Florian"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGMF003"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB (<a
                     href=""urn:local:.:5y-i78r0bc"">pGMF003</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:7o5-d1z2kwz"">Final L2 for
                     targeting PDS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:au-i9anmpc"">L2 Arabidopsis
                     PDS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:12-inuhtxy"">pGMF002a</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:ix-eboo9ps"">pICSL4723 L2 backbone
                     - pICSL11059 35Sp::HPTII:35St - pFH52 2x35Sp::SpCas9:NOSt
                     - pGMF001 with PDS target1 - pGMF002 with PDS
                     target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:16-ihwey0g"">CasD Level 1 P2
                     OsUbiP:Cas9:NosT</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:pt-i78tdbj"">L2 Nipponbare
                     PDS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:kf-i78t3nu"">L2 Arabidopsis
                     GL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:1d4-dvu4edz"">pICSL4723 L2 backbone
                     - pGMF006 - pFH52 2x35Sp::SpCas9:NOSt - pGMF001 with PDS
                     target1 - pGMF002 with PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; LB
                     (<a href=""urn:local:.:7jv-d1yz8b0"">pGMF003 </a>)"
                     /label="LB"
     primer_bind     582..605
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="1214708522571800e56245e6-7d99-4623-a835
                     -b224457251f3"
                     /created_by="primer3"
                     /Sequence="CTGGTGGCAGGATATATTGTGGTG"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pGMF6"
                     /label="GMF002"
     promoter        719..2056
                     /vntifkey=29
                     /modified_by="Florian"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L1 35Sp::eGFP-NLS:35St "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 35S
                     promoter (Cauliflower Mosaic Virus) (<a
                     href=""urn:local:.:v-dkb2238"">L1 35Sp::eGFP-NLS:35St
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Promoter, 35S (Cauliflower Mosaic Virus) (<a
                     href=""urn:local:.:7o5-d1z2kwz"">Final L2 for targeting
                     PDS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Promoter, 35S (Cauliflower Mosaic Virus) (<a
                     href=""urn:local:.:7jr-d1yz7zq"">pICSL11059
                     35Sp::HPTII:35St</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Promoter, 35s (Cauliflower Mosaic Virus) (<a
                     href=""urn:local:.:f-c2iltpe"">pICSL11059</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Promoter, 35s (Cauliflower Mosaic Virus) (<a
                     href=""urn:local:.:60-c2iy93f"">Level2 Plantain PDS KO
                     vector final assembly</a>)"
                     /label="35S promoter (Cauliflower Mosaic Virus)"
     5'_UTR          2073..2134
                     /vntifkey=52
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pICSL11059"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Promoter, 35s (Cauliflower Mosaic Virus) (<a
                     href=""urn:local:.:f-c2iltpe"">pICSL11059</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Promoter, 35s (Cauliflower Mosaic Virus) (<a
                     href=""urn:local:.:60-c2iy93f"">Level2 Plantain PDS KO
                     vector final assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TMV
                     Omega (<a href=""urn:local:.:v-dkb2238"">L1
                     35Sp::eGFP-NLS:35St </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TMV
                     Omega (<a href=""urn:local:.:60-c2iy93f"">Level2 Plantain
                     PDS KO vector final assembly</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TMV
                     Omega (<a href=""urn:local:.:7o5-d1z2kwz"">Final L2 for
                     targeting PDS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TMV
                     Omega (<a href=""urn:local:.:7o5-d1z2kwz"">Final L2 for
                     targeting PDS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TMV
                     Omega (<a href=""urn:local:.:7jr-d1yz7zq"">pICSL11059
                     35Sp::HPTII:35St</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TMV
                     Omega (<a href=""urn:local:.:w-c2ilwp9"">pFH52</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; TMV
                     Omega (<a href=""urn:local:.:7jz-d1yz8pq"">pFH52
                     2x35Sp::SpCas9:NOSt</a>)"
                     /label="Promoter, 35s (Cauliflower Mosaic Virus)"
     CDS             2144..2860
                     /vntifkey=4
                     /modified_by="Florian"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L1 35Sp::eGFP-NLS:35St "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; eGFP (<a
                     href=""urn:local:.:v-dkb2238"">L1 35Sp::eGFP-NLS:35St
                     </a>)"
                     /label="eGFP"
     CDS             2867..3130
                     /codon_start=1
                     /note="/created_by=User"
                     /Original_Translation="AGSGAGGEFKREEQARKAKVNNEKKTEIVKPESCS
                     NEGDVKDLKRKDSEDGNEGEEEEASSKPKKPKVALSHLQDIDDTEADQEEE*"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L1 35Sp::eGFP-NLS:35St "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7-NLS
                     (<a href=""urn:local:.:v-dkb2238"">L1 35Sp::eGFP-NLS:35St
                     </a>)"
                     /modified_by="Florian"
                     /label="N7-NLS"
     terminator      3135..3338
                     /vntifkey=43
                     /modified_by="Florian"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L1 35Sp::eGFP-NLS:35St "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 3'
                     untranslated region and terminator, 35S (Cauliflower
                     Mosaic Virus) (<a href=""urn:local:.:v-dkb2238"">L1
                     35Sp::eGFP-NLS:35St </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3'
                     untranslated region and terminator, 35S (Cauliflower
                     Mosaic Virus) (<a href=""urn:local:.:7o5-d1z2kwz"">Final
                     L2 for targeting PDS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3'
                     untranslated region and terminator, 35S (Cauliflower
                     Mosaic Virus) (<a
                     href=""urn:local:.:7jr-d1yz7zq"">pICSL11059
                     35Sp::HPTII:35St</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3'
                     untranslated region and terminator, 35s (Cauliflower
                     Mosaic Virus) (<a
                     href=""urn:local:.:f-c2iltpe"">pICSL11059</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; 3'
                     untranslated region and terminator, 35s (Cauliflower
                     Mosaic Virus) (<a href=""urn:local:.:60-c2iy93f"">Level2
                     Plantain PDS KO vector final assembly</a>)"
                     /label="3' untranslated region and terminator, 35S
                     (Cauliflower Mosaic Virus)"
     primer_bind     3195..3214
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="51.2"
                     /annotation_group="121470859000860019a42151-ca9c-4d21-8b3c
                     -32a22d8679c6"
                     /created_by="primer3"
                     /Sequence="AGATAAGGGAATTAGGGTTC"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pGMF6"
                     /label="GMF003"
     Misc._feature   complement(3370..3497)
                     /vntifkey=21
                     /note="Unknown feature type:Misc._feature"
                     /note="Derived using Geneious Prime 2021.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pICSL11059 35Sp::HPTII:35St"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:7jr-d1yz7zq"">pICSL11059
                     35Sp::HPTII:35St</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:w-c2ilwp9"">pFH52</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:f-c2iltpe"">pICSL11059</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:7jz-d1yz8pq"">pFH52
                     2x35Sp::SpCas9:NOSt</a>)"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGMF001 with PDS target1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RB short
                     (<a href=""urn:local:.:7n4-d1z1usp"">pGMF001 with PDS
                     target1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:f-c2iltpe"">pICSL11059</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:av-i9anmpc"">pGMF002 with
                     Arabidopsis PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:5e-i9amgby"">pGMF001 with
                     Arabidopsis GL1 target1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:y-i5qen40"">pGMF001a</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:5t-i78r0bc"">pGMF001 with
                     Arabidopsis PDS target1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:5g-i9amgby"">pGMF002 with
                     Arabidopsis GL1 target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:12-inuhtxy"">pGMF002a</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:d8-i78s4q6"">pGMF002 with
                     Arabidopsis GL1 target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; RB
                     short (<a href=""urn:local:.:o-ihwbmvz"">CasB pFH66</a>)"
                     /label="RB short"
     primer_bind     complement(3484..3509)
                     /%GC="53.8"
                     /Hairpin_Tm="54.9"
                     /Self_Dimer_Tm="21.4"
                     /Tm="66.5"
                     /annotation_group="1214706659061000f775865d-c98f-4f61-83e6
                     -4e3854883742"
                     /created_by="primer3"
                     /Sequence="GAACCCTGTGGTTGGCATGCACATAC"
                     /Mismatches=0
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="pGMF6"
                     /label="GMF001"
     rep_origin      complement(3946..4560)
                     /vntifkey=21
                     /note="Derived using Geneious Prime 2020.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pUCIDT (Amp)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; rep
                     (pMB1) (<a href=""urn:local:.:1s-ce2z606"">pUCIDT
                     (Amp)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     rep (pMB1) (<a
                     href=""urn:local:.:7jn-d1yz5cw"">pGMF002</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:-11/15;
                     ori (<a href=""urn:local:.:ix-cgdmhmr"">pUCIDT-Amp
                     GoldenGate </a>)"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGMF001 with PDS target1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; rep
                     (pMB1) (<a href=""urn:local:.:7n4-d1z1usp"">pGMF001 with
                     PDS target1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; rep
                     (pMB1) (<a href=""urn:local:.:av-i9anmpc"">pGMF002 with
                     Arabidopsis PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; rep
                     (pMB1) (<a
                     href=""urn:local:.:j-hwkax9k"">pGMF002a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; rep
                     (pMB1) (<a href=""urn:local:.:5e-i9amgby"">pGMF001 with
                     Arabidopsis GL1 target1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; rep
                     (pMB1) (<a href=""urn:local:.:5t-i78r0bc"">pGMF001 with
                     Arabidopsis PDS target1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; rep
                     (pMB1) (<a href=""urn:local:.:5g-i9amgby"">pGMF002 with
                     Arabidopsis GL1 target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; rep
                     (pMB1) (<a href=""urn:local:.:d8-i78s4q6"">pGMF002 with
                     Arabidopsis GL1 target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; rep
                     (pMB1) (<a href=""urn:local:.:7nn-d1z224o"">pGMF002 with
                     PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; rep
                     (pMB1) (<a href=""urn:local:.:cb-i78s2sv"">pGMF002 with
                     Nipponbare PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; rep
                     (pMB1) (<a href=""urn:local:.:5w-i78r0bc"">pGMF002 with
                     Arabidopsis PDS target2</a>)"
                     /label="rep (pMB1)"
     CDS             complement(4720..5580)
                     /Application_Notes="Allows E. coli to be resistant to
                     ampiciIIin."
                     /Full_Name="Ampicillin resistance gene"
                     /References="Proc Natl Acad Sci U S A. 75: 3737 (1978)"
                     /Selection_Drug="Ampicillin"
                     /Subtype="E. coli selection marker"
                     /Usage="Positive selection"
                     /__SeqFeature__="True"
                     /__level__=0
                     /application_notes="Allows E. coli to be resistant to
                     ampicillin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /name="Ampicillin"
                     /note="color: #c54b7c; direction: LEFT"
                     /official_designation="Ampicillin"
                     /uuid="39ee7c4ce93d4075b0df0df5df6b9861"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGMF001a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ampicillin (<a
                     href=""urn:local:.:y-i5qen40"">pGMF001a</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ampicillin (<a
                     href=""urn:local:.:12-inuhtxy"">pGMF002a</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ampicillin (<a
                     href=""urn:local:.:n-hwkaxtn"">pGMF001a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     signal sequence (<a
                     href=""urn:local:.:ix-cgdmhmr"">pUCIDT-Amp GoldenGate
                     </a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:17-c2in4sl"">pTwist Amp High
                     Copy</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:12-ihwebp0"">pAGM51323</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:16-ihwey0g"">CasD Level 1 P2
                     OsUbiP:Cas9:NosT</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:1s-ce2z606"">pUCIDT (Amp)</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:x-ihwbnpt"">pEPQD1CB0001</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:52-c2iy3lf"">L1_target1 in pTwist
                     Amp High Copy - fake primer PDS target1</a>)"
                     /label="Ampicillin"
     promoter        complement(5581..5685)
                     /created_by="hahnf"
                     /note="Derived using Geneious Prime 2020.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pUCIDT (Amp)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:1s-ce2z606"">pUCIDT
                     (Amp)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:ix-cgdmhmr"">pUCIDT-Amp GoldenGate
                     </a>)"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGMF001 with PDS target1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:7n4-d1z1usp"">pGMF001 with
                     PDS target1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:av-i9anmpc"">pGMF002 with
                     Arabidopsis PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:j-hwkax9k"">pGMF002a_with_Arabidopsis_U
                     6-26_promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:5e-i9amgby"">pGMF001 with
                     Arabidopsis GL1 target1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:5t-i78r0bc"">pGMF001 with
                     Arabidopsis PDS target1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:5g-i9amgby"">pGMF002 with
                     Arabidopsis GL1 target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:d8-i78s4q6"">pGMF002 with
                     Arabidopsis GL1 target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:7nn-d1z224o"">pGMF002 with
                     PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:cb-i78s2sv"">pGMF002 with
                     Nipponbare PDS target2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:5w-i78r0bc"">pGMF002 with
                     Arabidopsis PDS target2</a>)"
                     /label="AmpR promoter"
ORIGIN      
        1 tcgcgcgttt cggtgcacga ggccctttca tctcgcgcgt ttcggtgatg acggtgaaaa
       61 cctctgacac atgcagctcc cctagacggt cacagcttgt ctgtaagcgg atgccgggag
      121 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      181 tgcggcatca gagcagattg tactgagagt gcaccaaatg cggtgtgaaa taccgcacag
      241 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
      301 ggaagggcga tcggtgcggg cctcatcgct attacgccag ctggcgaaag ggggatgtgc
      361 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      421 ggccagtgca acgcgatgac gatggatagc gattcatcga tgagctgacc cgatcgccgc
      481 cgccggaggg ttgcgtttga gacaggcgac agatactgat gggctgcctg tatcgagtgg
      541 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
      601 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
      661 ctggggtgga tgcagtgggc cccactctgt gaagacaatg ccgaattcgg atccggagga
      721 attccaatcc cacaaaaatc tgagcttaac agcacagttg ctcctctcag agcagaatcg
      781 ggtattcaac accctcatat caactactac gttgtgtata acggtccaca tgccggtata
      841 tacgatgact ggggttgtac aaaggcggca acaaacggcg ttcccggagt tgcacacaag
      901 aaatttgcca ctattacaga ggcaagagca gcagctgacg cgtacacaac aagtcagcaa
      961 acagacaggt tgaacttcat ccccaaagga gaagctcaac tcaagcccaa gagctttgct
     1021 aaggccctaa caagcccacc aaagcaaaaa gcccactggc tcacgctagg aaccaaaagg
     1081 cccagcagtg atccagcccc aaaagagatc tcctttgccc cggagattac aatggacgat
     1141 ttcctctatc tttacgatct aggaaggaag ttcgaaggtg aaggtgacga cactatgttc
     1201 accactgata atgagaaggt tagcctcttc aatttcagaa agaatgctga cccacagatg
     1261 gttagagagg cctacgcagc aagtctcatc aagacgatct acccgagtaa caatctccag
     1321 gagatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaattgc
     1381 atcaagaaca cagagaaaga catatttctc aagatcagaa gtactattcc agtatggacg
     1441 attcaaggct tgcttcataa accaaggcaa gtaatagaga ttggagtctc taaaaaggta
     1501 gttcctactg aatctaaggc catgcatgga gtctaagatt caaatcgagg atctaacaga
     1561 actcgccgtc aagactggcg aacagttcat acagagtctt ttacgactca atgacaagaa
     1621 gaaaatcttc gtcaacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga
     1681 tacagtctca gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa
     1741 cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga
     1801 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc
     1861 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     1921 ggttccaacc acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga
     1981 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     2041 tttggagagg acacgctcga gtataagagc tcatttttac aacaattacc aacaacaaca
     2101 aacaacaaac aacattacaa ttacatttac aattatcgat acaatggtga gcaagggcga
     2161 ggagctgttc accggcgtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2221 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2281 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2341 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2401 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2461 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2521 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2581 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2641 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2701 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2761 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2821 cgccgccggg atcactctcg gcatggacga gctgtacaag ggaggtgccg gcagtggcgc
     2881 tggcggagaa tttaagcgcg aagaacaagc ccgcaaggct aaagttaata acgaaaaaaa
     2941 gaccgaaatc gtcaagccag agtcttgctc caatgagggc gatgtgaagg atcttaaaag
     3001 gaaagactct gaggatggaa atgaagggga ggaagaagag gcttcgagta agcccaagaa
     3061 accgaaggtc gcactgagtc acttgcagga catagatgat acggaagctg accaagaaga
     3121 agaataatga gcttctctag ctagagtcga tcgacaagct cgagtttctc cataataatg
     3181 tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag
     3241 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     3301 taattcctaa aaccaaaatc cagtactaaa atccagatcg ctgcaattgt cttctgcacg
     3361 aagtggttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
     3421 agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
     3481 tttgtatgtg catgccaacc acagggttcc atcagttctg gaccagcgag ctgtgctgcg
     3541 actcgtggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3601 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3661 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3721 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3781 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4081 ctctcctgtt ccgaccctgt cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4381 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4561 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4621 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4681 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4741 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4801 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4861 agatccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4921 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4981 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5041 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5101 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5161 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5221 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5281 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5341 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5401 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5461 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5521 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5581 actctacctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5641 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5701 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5761 tatcacgagg ccctttcatc
//
