LOCUS       567_PBbsr2-CAG-ARID1A- 10790 bp ds-DNA     circular     09-JUL-2024
DEFINITION  .
KEYWORDS    "creator:GenScript" "marker:AmpR"
FEATURES             Location/Qualifiers
     primer_bind     142..158
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     promoter        168..186
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     enhancer        571..950
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
     promoter        953..1228
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     source          2009..2136
                     /label="source"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     regulatory      2276..2284
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) regulatory_class: other: "
     misc_feature    2282..2284
                     /label="IDR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2282..6229
                     /label="ARID1A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2282..6304
                     /label="ARID1A"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGDVKLTPATKMNNKADGTPKTESKSKKSSSSTTTNEKITKLYELGGEPERKMWVDRYLAFTEEKAMGMTNLPAVGRKPLDLYRLYVSVKEIGGLTQVNKNKKWRELATNLNVGTSSSAASSLKKQYIQCLYAFECKIERGEDPPPDIFAAADSKKSQPKIQPPSPAGSGSMQGPQTPQSTSSSMAEGGDLKPPTPASTPHSQIPPLPGMSRSNSVGIQDAFNDGSDSTFQKRNSMTPNPGYQPSMNTSDMMGRMSYEPNKDPYGSMRKAPGSDPFMSSGQGPNGGMGDPYSRAAGPGLGNVAMGPRQHYPYGGPYDRVRTEPGIGPEGNMSTGAPQPNLMPSNPDSGMYSPSRYPPQQQQQQQQRHDSYGNQFSTQGTPSGSPFPSQQTTMYQQQQQNYKRPMDGTYGPPAKRHEGEMYSVPYSTGQGQPQQQQLPPAQPQPASQQQAAQPSPQQDVYNQYGNAYPATATAATERRPAGGPQNQFPFQFGRDRVSAPPGTNAQQNMPPQMMGGPIQASAEVAQQGTMWQGRNDMTYNYANRQSTGSAPQGPAYHGVNRTDEMLHTDQRANHEGSWPSHGTRQPPYGPSAPVPPMTRPPPSNYQPPPSMQNHIPQVSSPAPLPRPMENRTSPSKSPFLHSGMKMQKAGPPVPASHIAPAPVQPPMIRRDITFPPGSVEATQPVLKQRRRLTMKDIGTPEAWRVMMSLKSGLLAESTWALDTINILLYDDNSIMTFNLSQLPGLLELLVEYFRRCLIEIFGILKEYEVGDPGQRTLLDPGRFSKVSSPAPMEGGEEEEELLGPKLEEEEEEEVVENDEEIAFSGKDKPASENSEEKLISKFDKLPVKIVQKNDPFVVDCSDKLGRVQEFDSGLLHWRIGGGDTTEHIQTHFESKTELLPSRPHAPCPPAPRKHVTTAEGTPGTTDQEGPPPDGPPEKRITATMDDMLSTRSSTLTEDGAKSSEAIKESSKFPFGISPAQSHRNIKILEDEPHSKDETPLCTLLDWQDSLAKRCVCVSNTIRSLSFVPGNDFEMSKHPGLLLILGKLILLHHKHPERKQAPLTYEKEEEQDQGVSCNKVEWWWDCLEMLRENTLVTLANISGQLDLSPYPESICLPVLDGLLHWAVCPSAEAQDPFSTLGPNAVLSPQRLVLETLSKLSIQDNNVDLILATPPFSRLEKLYSTMVRFLSDRKNPVCREMAVVLLANLAQGDSLAARAIAVQKGSIGNLLGFLEDSLAATQFQQSQASLLHMQNPPFEPTSVDMMRRAARALLALAKVDENHSEFTLYESRLLDISVSPLMNSLVSQVICDVLFLIGQSTGDYKDHDGDYKDHDIDYKDDDDK*"
     misc_feature    2285..2881
                     /label="ARID"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2882..4204
                     /label="IDR2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4205..6229
                     /label="CBR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             6236..6301
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site transl_table: 1 translation: DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6396..6945
                     /label="IRES"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     polyA_signal    7422..7646
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: bovine growth hormone polyadenylation signal"
     primer_bind     complement(7693..7709)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    7717..7733
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7741..7747)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(7748..7765)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(7766..7771)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    7786..7807
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        complement(8144..8162)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8183..8199)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    8207..8223
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8231..8237)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(8238..8255)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(8256..8261)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    8276..8297
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     rep_origin      complement(8585..9173)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(9344..10135)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(10136..10204)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(10205..10309)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     rep_origin      complement(10335..10790)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      181 tatagggcga attggggcgc gccattctag attaacccta gaaagatagt ctgcgtaaaa
      241 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
      301 tgcatttaGG ACATCTCAGT CGCCGcttgg agctcccgtg aggcgtgctt gtcaatgcgg
      361 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
      421 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
      481 tacgtgataa cttattatat atatattttc ttgttataga tatcaactag aatgCTAGAC
      541 TCGAGAAGCT TGATCGGCCA CGCGGATCTC GACATTGATT ATTGACTAGT TATTAATAGT
      601 AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA
      661 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA
      721 CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT
      781 TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT CCGCCCCCTA
      841 TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTACGGG
      901 ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GGTCGAGGTG
      961 AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT
     1021 TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG GGGGGGGGGG GGCGCGCGCC
     1081 AGGCGGGGCG GGGCGGGGCG AGGGGCGGGG CGGGGCGAGG CGGAGAGGTG CGGCGGCAGC
     1141 CAATCAGAGC GGCGCGCTCC GAAAGTTTCC TTTTATGGCG AGGCGGCGGC GGCGGCGGCC
     1201 CTATAAAAAG CGAAGCGCGC GGCGGGCGGG AGTCGCTGCG TTGCCTTCGC CCCGTGCCCC
     1261 GCTCCGCGCC GCCTCGCGCC GCCCGCCCCG GCTCTGACTG ACCGCGTTAC TCCCACAGGT
     1321 GAGCGGGCGG GACGGCCCTT CTCCTCCGGG CTGTAATTAG CGCTTGGTTT AATGACGGCT
     1381 CGTTTCTTTT CTGTGGCTGC GTGAAAGCCT TAAAGGGCTC CGGGAGGGCC CTTTGTGCGG
     1441 GGGGGAGCGG CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCC
     1501 CGCGCTGCCC GGCGGCTGTG AGCGCTGCGG GCGCGGCGCG GGGCTTTGTG CGCTCCGCGT
     1561 GTGCGCGAGG GGAGCGCGGC CGGGGGCGGT GCCCCGCGGT GCGGGGGGGC TGCGAGGGGA
     1621 ACAAAGGCTG CGTGCGGGGT GTGTGCGTGG GGGGGTGAGC AGGGGGTGTG GGCGCGGCGG
     1681 TCGGGCTGTA ACCCCCCCCT GCACCCCCCT CCCCGAGTTG CTGAGCACGG CCCGGCTTCG
     1741 GGTGCGGGGC TCCGTGCGGG GCGTGGCGCG GGGCTCGCCG TGCCGGGCGG GGGGTGGCGG
     1801 CAGGTGGGGG TGCCGGGCGG GGCGGGGCCG CCTCGGGCCG GGGAGGGCTC GGGGGAGGGG
     1861 CGCGGCGGCC CCGGAGCGCC GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT
     1921 TATGGTAATC GTGCGAGAGG GCGCAGGGAC TTCCTTTGTC CCAAATCTGG CGGAGCCGAA
     1981 ATCTGGGAGG CGCCGCCGCA CCCCCTCTag cgggcgcggg gcgaagcggt gcggcgccgg
     2041 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     2101 tctccagcct cggggctgtc cgcgggggga cggctgCCTT CGGGGGGGAC GGGGCAGGGC
     2161 GGGGTTCGGC TTCTGGCGTG TGACCGGCGG CTCTAGCCGG AGCGCACGTC GGCAGTCGGC
     2221 TCCCTCGTTG ACCGAATCAC CGACCTCTCT CCCCAGGGGG ATCCACCGGG CTAGCGCCAC
     2281 CATGGGGGAT GTAAAGTTAA CTCCAGCCAC CAAAATGAAC AACAAGGCAG ATGGGACACC
     2341 CAAGACAGAA TCCAAATCCA AGAAATCCAG TTCTTCTACT ACAACCAATG AGAAGATCAC
     2401 CAAGTTGTAT GAGCTGGGTG GTGAGCCTGA GAGGAAGATG TGGGTGGACC GTTATCTGGC
     2461 CTTCACTGAG GAGAAGGCCA TGGGCATGAC AAATCTGCCT GCTGTGGGTA GGAAACCTCT
     2521 GGACCTCTAT CGCCTCTATG TGTCTGTGAA GGAGATTGGT GGATTGACTC AGGTCAACAA
     2581 GAACAAAAAA TGGCGGGAAC TTGCAACCAA CCTCAATGTG GGCACATCAA GCAGTGCTGC
     2641 CAGCTCCTTG AAAAAGCAGT ATATCCAGTG TCTCTATGCC TTTGAATGCA AGATTGAACG
     2701 GGGAGAAGAC CCTCCCCCAG ACATCTTTGC AGCTGCTGAT TCCAAGAAGT CCCAGCCCAA
     2761 GATCCAGCCT CCCTCTCCTG CGGGATCAGG ATCTATGCAG GGGCCCCAGA CTCCCCAGTC
     2821 AACCAGCAGT TCCATGGCAG AAGGAGGAGA CTTAAAGCCA CCAACTCCAG CATCCACACC
     2881 ACACAGTCAG ATCCCCCCAT TGCCAGGCAT GAGCAGGAGC AATTCAGTTG GGATCCAGGA
     2941 TGCCTTTAAT GATGGAAGTG ACTCCACATT CCAGAAGCGG AATTCCATGA CTCCAAACCC
     3001 TGGGTATCAG CCCAGTATGA ATACCTCTGA CATGATGGGG CGCATGTCCT ATGAGCCAAA
     3061 TAAGGATCCT TATGGCAGCA TGAGGAAAGC TCCAGGGAGT GATCCCTTCA TGTCCTCAGG
     3121 GCAGGGCCCC AACGGCGGGA TGGGTGACCC CTACAGTCGT GCTGCCGGCC CTGGGCTAGG
     3181 AAATGTGGCG ATGGGACCAC GACAGCACTA TCCCTATGGA GGTCCTTATG ACAGAGTGAG
     3241 GACGGAGCCT GGAATAGGGC CTGAGGGAAA CATGAGCACT GGGGCCCCAC AGCCGAATCT
     3301 CATGCCTTCC AACCCAGACT CGGGGATGTA TTCTCCTAGC CGCTACCCCC CGCAGCAGCA
     3361 GCAGCAGCAG CAGCAACGAC ATGATTCCTA TGGCAATCAG TTCTCCACCC AAGGCACCCC
     3421 TTCTGGCAGC CCCTTCCCCA GCCAGCAGAC TACAATGTAT CAACAGCAAC AGCAGAATTA
     3481 CAAGCGGCCA ATGGATGGCA CATATGGCCC TCCTGCCAAG CGGCACGAAG GGGAGATGTA
     3541 CAGCGTGCCA TACAGCACTG GGCAGGGGCA GCCTCAGCAG CAGCAGTTGC CCCCAGCCCA
     3601 GCCCCAGCCT GCCAGCCAGC AACAAGCTGC CCAGCCTTCC CCTCAGCAAG ATGTATACAA
     3661 CCAGTATGGC AATGCCTATC CTGCCACTGC CACAGCTGCT ACTGAGCGCC GACCAGCAGG
     3721 CGGCCCCCAG AACCAATTTC CATTCCAGTT TGGCCGAGAC CGTGTCTCTG CACCCCCTGG
     3781 CACCAATGCC CAGCAAAACA TGCCACCACA AATGATGGGC GGCCCCATAC AGGCATCAGC
     3841 TGAGGTTGCT CAGCAAGGCA CCATGTGGCA GGGGCGTAAT GACATGACCT ATAATTATGC
     3901 CAACAGGCAG AGCACGGGCT CTGCCCCCCA GGGCCCCGCC TATCATGGCG TGAACCGAAC
     3961 AGATGAAATG CTGCACACAG ATCAGAGGGC CAACCACGAA GGCTCGTGGC CTTCCCATGG
     4021 CACACGCCAG CCCCCATATG GTCCCTCTGC CCCTGTGCCC CCCATGACAA GGCCCCCTCC
     4081 ATCTAACTAC CAGCCCCCAC CAAGCATGCA GAATCACATT CCTCAGGTAT CCAGCCCTGC
     4141 TCCCCTGCCC CGGCCAATGG AGAACCGCAC CTCTCCTAGC AAGTCTCCAT TCCTGCACTC
     4201 TGGGATGAAA ATGCAGAAGG CAGGTCCCCC AGTACCTGCC TCGCACATAG CACCTGCCCC
     4261 TGTGCAGCCC CCCATGATTC GGCGGGATAT CACCTTCCCA CCTGGCTCTG TTGAAGCCAC
     4321 ACAGCCTGTG TTGAAGCAGA GGAGGCGGCT CACAATGAAA GACATTGGAA CCCCGGAGGC
     4381 ATGGCGGGTA ATGATGTCCC TCAAGTCTGG TCTCCTGGCA GAGAGCACAT GGGCATTAGA
     4441 TACCATCAAC ATCCTGCTGT ATGATGACAA CAGCATCATG ACCTTCAACC TCAGTCAGCT
     4501 CCCAGGGTTG CTAGAGCTCC TTGTAGAATA TTTCCGACGA TGCCTGATTG AGATCTTTGG
     4561 CATTTTAAAG GAGTATGAGG TGGGTGACCC AGGACAGAGA ACGCTACTGG ATCCTGGGAG
     4621 GTTCAGCAAG GTGTCTAGTC CAGCTCCCAT GGAGGGTGGG GAAGAAGAAG AAGAACTTCT
     4681 AGGTCCTAAA CTAGAAGAGG AAGAAGAAGA GGAAGTAGTT GAAAATGATG AGGAGATAGC
     4741 CTTTTCAGGC AAGGACAAGC CAGCTTCAGA GAATAGTGAG GAGAAGCTGA TCAGTAAGTT
     4801 TGACAAGCTT CCAGTAAAGA TCGTACAGAA GAATGATCCA TTTGTGGTGG ACTGCTCAGA
     4861 TAAGCTTGGG CGTGTGCAGG AGTTTGACAG TGGCCTGCTG CACTGGCGGA TTGGTGGGGG
     4921 GGACACCACT GAGCATATCC AGACCCACTT CGAGAGCAAG ACAGAGCTGC TGCCTTCCCG
     4981 GCCTCACGCA CCCTGCCCAC CAGCCCCTCG GAAGCATGTG ACAACAGCAG AGGGTACACC
     5041 AGGGACAACA GACCAGGAGG GGCCCCCACC TGATGGACCT CCAGAAAAAC GGATCACAGC
     5101 CACTATGGAT GACATGTTGT CTACTCGGTC TAGCACCTTG ACCGAGGATG GAGCTAAGAG
     5161 TTCAGAGGCC ATCAAGGAGA GCAGCAAGTT TCCATTTGGC ATTAGCCCAG CACAGAGCCA
     5221 CCGGAACATC AAGATCCTAG AGGACGAACC CCACAGTAAG GATGAGACCC CACTGTGTAC
     5281 CCTTCTGGAC TGGCAGGATT CTCTTGCCAA GCGCTGCGTC TGTGTGTCCA ATACCATTCG
     5341 AAGCCTGTCA TTTGTGCCAG GCAATGACTT TGAGATGTCC AAACACCCAG GGCTGCTGCT
     5401 CATCCTGGGC AAGCTGATCC TGCTGCACCA CAAGCACCCA GAACGGAAGC AGGCACCACT
     5461 AACTTATGAA AAGGAGGAGG AACAGGACCA AGGGGTGAGC TGCAACAAAG TGGAGTGGTG
     5521 GTGGGACTGC TTGGAGATGC TCCGGGAAAA CACCTTGGTT ACACTCGCCA ACATCTCGGG
     5581 GCAGTTGGAC CTATCTCCAT ACCCCGAGAG CATTTGCCTG CCTGTCCTGG ACGGACTCCT
     5641 ACACTGGGCA GTTTGCCCTT CAGCTGAAGC CCAGGACCCC TTTTCCACCC TGGGCCCCAA
     5701 TGCCGTCCTT TCCCCGCAGA GACTGGTCTT GGAAACCCTC AGCAAACTCA GCATCCAGGA
     5761 CAACAATGTG GACCTGATTC TGGCCACACC CCCCTTCAGC CGCCTGGAGA AGTTGTATAG
     5821 CACTATGGTG CGCTTCCTCA GTGACCGAAA GAACCCGGTG TGCCGGGAGA TGGCTGTGGT
     5881 ACTGCTGGCC AACCTGGCTC AGGGGGACAG CCTGGCAGCT CGTGCCATTG CAGTGCAGAA
     5941 GGGCAGTATC GGCAACCTCC TGGGCTTCCT AGAGGACAGC CTTGCCGCCA CACAGTTCCA
     6001 GCAGAGCCAG GCCAGCCTCC TCCACATGCA GAACCCACCC TTTGAGCCAA CTAGTGTGGA
     6061 CATGATGCGG CGGGCTGCCC GCGCGCTGCT TGCCTTGGCC AAGGTGGACG AGAACCACTC
     6121 AGAGTTTACT CTGTACGAAT CACGGCTGTT GGACATCTCG GTATCACCGC TGATGAATAG
     6181 CCTCGTGTCA CAAGTCATTT GTGATGTACT GTTTTTGATT GGCCAGTCCA CCGGTGACTA
     6241 CAAAGACCAT GACGGTGATT ATAAAGATCA TGATATCGAT TACAAGGATG ACGATGACAA
     6301 GTGAACGCGT TAGTGCGGAT CCTAGTGCGA ATTCTAGTGC GTCGACTCTA Gcaacggttt
     6361 ccctctagcg ggatcaattc cgcccccccc ccctaacgtt actggccgaa gccgcttgga
     6421 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     6481 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     6541 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     6601 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     6661 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     6721 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     6781 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     6841 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     6901 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg gtcatgaaaa
     6961 catttaacat ttctcaacaa gatctagaat tagtagaagt agcgacagag aagattacaa
     7021 tgctttatga ggataataaa catcatgtgg gagcggcaat tcgtacgaaa acaggagaaa
     7081 tcatttcggc agtacatatt gaagcgtata taggacgagt aactgtttgt gcagaagcca
     7141 ttgcgattgg tagtgcagtt tcgaatggac aaaaggattt tgacacgatt gtagctgtta
     7201 gacaccctta ttctgacgaa gtagatagaa gtattcgagt ggtaagtcct tgtggtatgt
     7261 gtagggagtt gatttcagac tatgcaccag attgttttgt gttaatagaa atgaatggca
     7321 agttagtcaa aactacgatt gaagaactca ttccactcaa atatacccga aattaaaagt
     7381 cgagatgcat cgatgatcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     7441 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     7501 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     7561 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     7621 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaacctgca gcccaagctt
     7681 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7741 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7801 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     7861 gatcttaaaa gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     7921 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     7981 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     8041 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     8101 gggttaatct agtatCGTAg Attaattaat ccagcttttg ttccctttag tgagggttaa
     8161 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8221 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8281 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8341 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8401 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8461 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8521 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8581 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8641 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8701 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8761 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8821 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8881 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8941 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9001 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     9061 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9121 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9181 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9241 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9301 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9361 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9421 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9481 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9541 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9601 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9661 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9721 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9781 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9841 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9901 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9961 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10021 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10081 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10141 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10201 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10261 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10321 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
    10381 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
    10441 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
    10501 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
    10561 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
    10621 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
    10681 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
    10741 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
//