LOCUS       568_pPBbsr2-CAG-ARID1A 10193 bp ds-DNA     circular     14-MAR-2025
DEFINITION  .
KEYWORDS    "creator:GenScript" "marker:AmpR"
FEATURES             Location/Qualifiers
     primer_bind     142..158
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     promoter        168..186
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     enhancer        571..950
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
     misc_feature    952..2193
                     /label="B-actin promoter "
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        953..1228
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     source          2009..2136
                     /label="source"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     regulatory      2276..2284
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) regulatory_class: other: "
     misc_feature    2282..2284
                     /label="IDR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2282..5632
                     /label="ARID1A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2282..5707
                     /label="ARID1A"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MHSQIPPLPGMSRSNSVGIQDAFNDGSDSTFQKRNSMTPNPGYQPSMNTSDMMGRMSYEPNKDPYGSMRKAPGSDPFMSSGQGPNGGMGDPYSRAAGPGLGNVAMGPRQHYPYGGPYDRVRTEPGIGPEGNMSTGAPQPNLMPSNPDSGMYSPSRYPPQQQQQQQQRHDSYGNQFSTQGTPSGSPFPSQQTTMYQQQQQNYKRPMDGTYGPPAKRHEGEMYSVPYSTGQGQPQQQQLPPAQPQPASQQQAAQPSPQQDVYNQYGNAYPATATAATERRPAGGPQNQFPFQFGRDRVSAPPGTNAQQNMPPQMMGGPIQASAEVAQQGTMWQGRNDMTYNYANRQSTGSAPQGPAYHGVNRTDEMLHTDQRANHEGSWPSHGTRQPPYGPSAPVPPMTRPPPSNYQPPPSMQNHIPQVSSPAPLPRPMENRTSPSKSPFLHSGMKMQKAGPPVPASHIAPAPVQPPMIRRDITFPPGSVEATQPVLKQRRRLTMKDIGTPEAWRVMMSLKSGLLAESTWALDTINILLYDDNSIMTFNLSQLPGLLELLVEYFRRCLIEIFGILKEYEVGDPGQRTLLDPGRFSKVSSPAPMEGGEEEEELLGPKLEEEEEEEVVENDEEIAFSGKDKPASENSEEKLISKFDKLPVKIVQKNDPFVVDCSDKLGRVQEFDSGLLHWRIGGGDTTEHIQTHFESKTELLPSRPHAPCPPAPRKHVTTAEGTPGTTDQEGPPPDGPPEKRITATMDDMLSTRSSTLTEDGAKSSEAIKESSKFPFGISPAQSHRNIKILEDEPHSKDETPLCTLLDWQDSLAKRCVCVSNTIRSLSFVPGNDFEMSKHPGLLLILGKLILLHHKHPERKQAPLTYEKEEEQDQGVSCNKVEWWWDCLEMLRENTLVTLANISGQLDLSPYPESICLPVLDGLLHWAVCPSAEAQDPFSTLGPNAVLSPQRLVLETLSKLSIQDNNVDLILATPPFSRLEKLYSTMVRFLSDRKNPVCREMAVVLLANLAQGDSLAARAIAVQKGSIGNLLGFLEDSLAATQFQQSQASLLHMQNPPFEPTSVDMMRRAARALLALAKVDENHSEFTLYESRLLDISVSPLMNSLVSQVICDVLFLIGQSTGDYKDHDGDYKDHDIDYKDDDDK*"
     misc_feature    2285..3607
                     /label="IDR2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3608..5632
                     /label="CBR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             5639..5704
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site transl_table: 1 translation: DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5799..6348
                     /label="IRES"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     polyA_signal    6825..7049
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: bovine growth hormone polyadenylation signal"
     primer_bind     complement(7096..7112)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    7120..7136
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7144..7150)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(7151..7168)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(7169..7174)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    7189..7210
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        complement(7547..7565)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7586..7602)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    7610..7626
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7634..7640)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(7641..7658)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(7659..7664)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    7679..7700
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     rep_origin      complement(7988..8576)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(8747..9538)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(9539..9607)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(9608..9712)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     rep_origin      complement(9738..10193)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      181 tatagggcga attggggcgc gccattctag attaacccta gaaagatagt ctgcgtaaaa
      241 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
      301 tgcatttaGG ACATCTCAGT CGCCGcttgg agctcccgtg aggcgtgctt gtcaatgcgg
      361 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
      421 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
      481 tacgtgataa cttattatat atatattttc ttgttataga tatcaactag aatgCTAGAC
      541 TCGAGAAGCT TGATCGGCCA CGCGGATCTC GACATTGATT ATTGACTAGT TATTAATAGT
      601 AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA
      661 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA
      721 CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT
      781 TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT CCGCCCCCTA
      841 TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTACGGG
      901 ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GGTCGAGGTG
      961 AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT
     1021 TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG GGGGGGGGGG GGCGCGCGCC
     1081 AGGCGGGGCG GGGCGGGGCG AGGGGCGGGG CGGGGCGAGG CGGAGAGGTG CGGCGGCAGC
     1141 CAATCAGAGC GGCGCGCTCC GAAAGTTTCC TTTTATGGCG AGGCGGCGGC GGCGGCGGCC
     1201 CTATAAAAAG CGAAGCGCGC GGCGGGCGGG AGTCGCTGCG TTGCCTTCGC CCCGTGCCCC
     1261 GCTCCGCGCC GCCTCGCGCC GCCCGCCCCG GCTCTGACTG ACCGCGTTAC TCCCACAGGT
     1321 GAGCGGGCGG GACGGCCCTT CTCCTCCGGG CTGTAATTAG CGCTTGGTTT AATGACGGCT
     1381 CGTTTCTTTT CTGTGGCTGC GTGAAAGCCT TAAAGGGCTC CGGGAGGGCC CTTTGTGCGG
     1441 GGGGGAGCGG CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCC
     1501 CGCGCTGCCC GGCGGCTGTG AGCGCTGCGG GCGCGGCGCG GGGCTTTGTG CGCTCCGCGT
     1561 GTGCGCGAGG GGAGCGCGGC CGGGGGCGGT GCCCCGCGGT GCGGGGGGGC TGCGAGGGGA
     1621 ACAAAGGCTG CGTGCGGGGT GTGTGCGTGG GGGGGTGAGC AGGGGGTGTG GGCGCGGCGG
     1681 TCGGGCTGTA ACCCCCCCCT GCACCCCCCT CCCCGAGTTG CTGAGCACGG CCCGGCTTCG
     1741 GGTGCGGGGC TCCGTGCGGG GCGTGGCGCG GGGCTCGCCG TGCCGGGCGG GGGGTGGCGG
     1801 CAGGTGGGGG TGCCGGGCGG GGCGGGGCCG CCTCGGGCCG GGGAGGGCTC GGGGGAGGGG
     1861 CGCGGCGGCC CCGGAGCGCC GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT
     1921 TATGGTAATC GTGCGAGAGG GCGCAGGGAC TTCCTTTGTC CCAAATCTGG CGGAGCCGAA
     1981 ATCTGGGAGG CGCCGCCGCA CCCCCTCTag cgggcgcggg gcgaagcggt gcggcgccgg
     2041 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     2101 tctccagcct cggggctgtc cgcgggggga cggctgCCTT CGGGGGGGAC GGGGCAGGGC
     2161 GGGGTTCGGC TTCTGGCGTG TGACCGGCGG CTCTAGCCGG AGCGCACGTC GGCAGTCGGC
     2221 TCCCTCGTTG ACCGAATCAC CGACCTCTCT CCCCAGGGGG ATCCACCGGG CTAGCGCCAC
     2281 CATGCACAGT CAGATCCCCC CATTGCCAGG CATGAGCAGG AGCAATTCAG TTGGGATCCA
     2341 GGATGCCTTT AATGATGGAA GTGACTCCAC ATTCCAGAAG CGGAATTCCA TGACTCCAAA
     2401 CCCTGGGTAT CAGCCCAGTA TGAATACCTC TGACATGATG GGGCGCATGT CCTATGAGCC
     2461 AAATAAGGAT CCTTATGGCA GCATGAGGAA AGCTCCAGGG AGTGATCCCT TCATGTCCTC
     2521 AGGGCAGGGC CCCAACGGCG GGATGGGTGA CCCCTACAGT CGTGCTGCCG GCCCTGGGCT
     2581 AGGAAATGTG GCGATGGGAC CACGACAGCA CTATCCCTAT GGAGGTCCTT ATGACAGAGT
     2641 GAGGACGGAG CCTGGAATAG GGCCTGAGGG AAACATGAGC ACTGGGGCCC CACAGCCGAA
     2701 TCTCATGCCT TCCAACCCAG ACTCGGGGAT GTATTCTCCT AGCCGCTACC CCCCGCAGCA
     2761 GCAGCAGCAG CAGCAGCAAC GACATGATTC CTATGGCAAT CAGTTCTCCA CCCAAGGCAC
     2821 CCCTTCTGGC AGCCCCTTCC CCAGCCAGCA GACTACAATG TATCAACAGC AACAGCAGAA
     2881 TTACAAGCGG CCAATGGATG GCACATATGG CCCTCCTGCC AAGCGGCACG AAGGGGAGAT
     2941 GTACAGCGTG CCATACAGCA CTGGGCAGGG GCAGCCTCAG CAGCAGCAGT TGCCCCCAGC
     3001 CCAGCCCCAG CCTGCCAGCC AGCAACAAGC TGCCCAGCCT TCCCCTCAGC AAGATGTATA
     3061 CAACCAGTAT GGCAATGCCT ATCCTGCCAC TGCCACAGCT GCTACTGAGC GCCGACCAGC
     3121 AGGCGGCCCC CAGAACCAAT TTCCATTCCA GTTTGGCCGA GACCGTGTCT CTGCACCCCC
     3181 TGGCACCAAT GCCCAGCAAA ACATGCCACC ACAAATGATG GGCGGCCCCA TACAGGCATC
     3241 AGCTGAGGTT GCTCAGCAAG GCACCATGTG GCAGGGGCGT AATGACATGA CCTATAATTA
     3301 TGCCAACAGG CAGAGCACGG GCTCTGCCCC CCAGGGCCCC GCCTATCATG GCGTGAACCG
     3361 AACAGATGAA ATGCTGCACA CAGATCAGAG GGCCAACCAC GAAGGCTCGT GGCCTTCCCA
     3421 TGGCACACGC CAGCCCCCAT ATGGTCCCTC TGCCCCTGTG CCCCCCATGA CAAGGCCCCC
     3481 TCCATCTAAC TACCAGCCCC CACCAAGCAT GCAGAATCAC ATTCCTCAGG TATCCAGCCC
     3541 TGCTCCCCTG CCCCGGCCAA TGGAGAACCG CACCTCTCCT AGCAAGTCTC CATTCCTGCA
     3601 CTCTGGGATG AAAATGCAGA AGGCAGGTCC CCCAGTACCT GCCTCGCACA TAGCACCTGC
     3661 CCCTGTGCAG CCCCCCATGA TTCGGCGGGA TATCACCTTC CCACCTGGCT CTGTTGAAGC
     3721 CACACAGCCT GTGTTGAAGC AGAGGAGGCG GCTCACAATG AAAGACATTG GAACCCCGGA
     3781 GGCATGGCGG GTAATGATGT CCCTCAAGTC TGGTCTCCTG GCAGAGAGCA CATGGGCATT
     3841 AGATACCATC AACATCCTGC TGTATGATGA CAACAGCATC ATGACCTTCA ACCTCAGTCA
     3901 GCTCCCAGGG TTGCTAGAGC TCCTTGTAGA ATATTTCCGA CGATGCCTGA TTGAGATCTT
     3961 TGGCATTTTA AAGGAGTATG AGGTGGGTGA CCCAGGACAG AGAACGCTAC TGGATCCTGG
     4021 GAGGTTCAGC AAGGTGTCTA GTCCAGCTCC CATGGAGGGT GGGGAAGAAG AAGAAGAACT
     4081 TCTAGGTCCT AAACTAGAAG AGGAAGAAGA AGAGGAAGTA GTTGAAAATG ATGAGGAGAT
     4141 AGCCTTTTCA GGCAAGGACA AGCCAGCTTC AGAGAATAGT GAGGAGAAGC TGATCAGTAA
     4201 GTTTGACAAG CTTCCAGTAA AGATCGTACA GAAGAATGAT CCATTTGTGG TGGACTGCTC
     4261 AGATAAGCTT GGGCGTGTGC AGGAGTTTGA CAGTGGCCTG CTGCACTGGC GGATTGGTGG
     4321 GGGGGACACC ACTGAGCATA TCCAGACCCA CTTCGAGAGC AAGACAGAGC TGCTGCCTTC
     4381 CCGGCCTCAC GCACCCTGCC CACCAGCCCC TCGGAAGCAT GTGACAACAG CAGAGGGTAC
     4441 ACCAGGGACA ACAGACCAGG AGGGGCCCCC ACCTGATGGA CCTCCAGAAA AACGGATCAC
     4501 AGCCACTATG GATGACATGT TGTCTACTCG GTCTAGCACC TTGACCGAGG ATGGAGCTAA
     4561 GAGTTCAGAG GCCATCAAGG AGAGCAGCAA GTTTCCATTT GGCATTAGCC CAGCACAGAG
     4621 CCACCGGAAC ATCAAGATCC TAGAGGACGA ACCCCACAGT AAGGATGAGA CCCCACTGTG
     4681 TACCCTTCTG GACTGGCAGG ATTCTCTTGC CAAGCGCTGC GTCTGTGTGT CCAATACCAT
     4741 TCGAAGCCTG TCATTTGTGC CAGGCAATGA CTTTGAGATG TCCAAACACC CAGGGCTGCT
     4801 GCTCATCCTG GGCAAGCTGA TCCTGCTGCA CCACAAGCAC CCAGAACGGA AGCAGGCACC
     4861 ACTAACTTAT GAAAAGGAGG AGGAACAGGA CCAAGGGGTG AGCTGCAACA AAGTGGAGTG
     4921 GTGGTGGGAC TGCTTGGAGA TGCTCCGGGA AAACACCTTG GTTACACTCG CCAACATCTC
     4981 GGGGCAGTTG GACCTATCTC CATACCCCGA GAGCATTTGC CTGCCTGTCC TGGACGGACT
     5041 CCTACACTGG GCAGTTTGCC CTTCAGCTGA AGCCCAGGAC CCCTTTTCCA CCCTGGGCCC
     5101 CAATGCCGTC CTTTCCCCGC AGAGACTGGT CTTGGAAACC CTCAGCAAAC TCAGCATCCA
     5161 GGACAACAAT GTGGACCTGA TTCTGGCCAC ACCCCCCTTC AGCCGCCTGG AGAAGTTGTA
     5221 TAGCACTATG GTGCGCTTCC TCAGTGACCG AAAGAACCCG GTGTGCCGGG AGATGGCTGT
     5281 GGTACTGCTG GCCAACCTGG CTCAGGGGGA CAGCCTGGCA GCTCGTGCCA TTGCAGTGCA
     5341 GAAGGGCAGT ATCGGCAACC TCCTGGGCTT CCTAGAGGAC AGCCTTGCCG CCACACAGTT
     5401 CCAGCAGAGC CAGGCCAGCC TCCTCCACAT GCAGAACCCA CCCTTTGAGC CAACTAGTGT
     5461 GGACATGATG CGGCGGGCTG CCCGCGCGCT GCTTGCCTTG GCCAAGGTGG ACGAGAACCA
     5521 CTCAGAGTTT ACTCTGTACG AATCACGGCT GTTGGACATC TCGGTATCAC CGCTGATGAA
     5581 TAGCCTCGTG TCACAAGTCA TTTGTGATGT ACTGTTTTTG ATTGGCCAGT CCACCGGTGA
     5641 CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGATATC GATTACAAGG ATGACGATGA
     5701 CAAGTGAACG CGTTAGTGCG GATCCTAGTG CGAATTCTAG TGCGTCGACT CTAGcaacgg
     5761 tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc gaagccgctt
     5821 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     5881 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5941 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     6001 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     6061 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     6121 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     6181 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     6241 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     6301 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc atggtcatga
     6361 aaacatttaa catttctcaa caagatctag aattagtaga agtagcgaca gagaagatta
     6421 caatgcttta tgaggataat aaacatcatg tgggagcggc aattcgtacg aaaacaggag
     6481 aaatcatttc ggcagtacat attgaagcgt atataggacg agtaactgtt tgtgcagaag
     6541 ccattgcgat tggtagtgca gtttcgaatg gacaaaagga ttttgacacg attgtagctg
     6601 ttagacaccc ttattctgac gaagtagata gaagtattcg agtggtaagt ccttgtggta
     6661 tgtgtaggga gttgatttca gactatgcac cagattgttt tgtgttaata gaaatgaatg
     6721 gcaagttagt caaaactacg attgaagaac tcattccact caaatatacc cgaaattaaa
     6781 agtcgagatg catcgatgat ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     6841 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6901 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6961 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7021 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacct gcagcccaag
     7081 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7141 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7201 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7261 gcggatctta aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa
     7321 taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat
     7381 ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa
     7441 acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt
     7501 ctagggttaa tctagtatCG TAgAttaatt aatccagctt ttgttccctt tagtgagggt
     7561 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7621 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7681 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7801 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7921 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7981 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8041 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8101 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8161 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8221 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8281 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8341 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8401 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8461 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8521 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8581 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8641 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8701 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8761 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8821 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8881 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8941 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9001 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9061 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9121 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9181 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9241 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9301 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9361 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9421 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9481 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9541 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9601 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9661 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9721 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     9781 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     9841 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     9901 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     9961 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
    10021 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
    10081 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
    10141 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgt
//