LOCUS       569_pPBbsr2-CAG-ARID1A- 8870 bp ds-DNA     circular     17-JUL-2024
DEFINITION  .
KEYWORDS    "creator:GenScript" "marker:AmpR"
FEATURES             Location/Qualifiers
     primer_bind     142..158
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     promoter        168..186
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     enhancer        571..950
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
     promoter        953..1228
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     source          2009..2136
                     /label="source"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     regulatory      2276..2284
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) regulatory_class: other: "
     misc_feature    2282..2284
                     /label="IDR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2282..4309
                     /label="ARID1A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2282..4384
                     /label="ARID1A"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MMKMQKAGPPVPASHIAPAPVQPPMIRRDITFPPGSVEATQPVLKQRRRLTMKDIGTPEAWRVMMSLKSGLLAESTWALDTINILLYDDNSIMTFNLSQLPGLLELLVEYFRRCLIEIFGILKEYEVGDPGQRTLLDPGRFSKVSSPAPMEGGEEEEELLGPKLEEEEEEEVVENDEEIAFSGKDKPASENSEEKLISKFDKLPVKIVQKNDPFVVDCSDKLGRVQEFDSGLLHWRIGGGDTTEHIQTHFESKTELLPSRPHAPCPPAPRKHVTTAEGTPGTTDQEGPPPDGPPEKRITATMDDMLSTRSSTLTEDGAKSSEAIKESSKFPFGISPAQSHRNIKILEDEPHSKDETPLCTLLDWQDSLAKRCVCVSNTIRSLSFVPGNDFEMSKHPGLLLILGKLILLHHKHPERKQAPLTYEKEEEQDQGVSCNKVEWWWDCLEMLRENTLVTLANISGQLDLSPYPESICLPVLDGLLHWAVCPSAEAQDPFSTLGPNAVLSPQRLVLETLSKLSIQDNNVDLILATPPFSRLEKLYSTMVRFLSDRKNPVCREMAVVLLANLAQGDSLAARAIAVQKGSIGNLLGFLEDSLAATQFQQSQASLLHMQNPPFEPTSVDMMRRAARALLALAKVDENHSEFTLYESRLLDISVSPLMNSLVSQVICDVLFLIGQSTGDYKDHDGDYKDHDIDYKDDDDK*"
     misc_feature    2285..4309
                     /label="CBR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4316..4381
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site transl_table: 1 translation: DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4476..5025
                     /label="IRES"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     polyA_signal    5502..5726
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: bovine growth hormone polyadenylation signal"
     primer_bind     complement(5773..5789)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    5797..5813
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5821..5827)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(5828..5845)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(5846..5851)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    5866..5887
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        complement(6224..6242)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6263..6279)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    6287..6303
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6311..6317)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(6318..6335)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(6336..6341)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    6356..6377
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     rep_origin      complement(6665..7253)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(7424..8215)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(8216..8284)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(8285..8389)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     rep_origin      complement(8415..8870)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      181 tatagggcga attggggcgc gccattctag attaacccta gaaagatagt ctgcgtaaaa
      241 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
      301 tgcatttaGG ACATCTCAGT CGCCGcttgg agctcccgtg aggcgtgctt gtcaatgcgg
      361 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
      421 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
      481 tacgtgataa cttattatat atatattttc ttgttataga tatcaactag aatgCTAGAC
      541 TCGAGAAGCT TGATCGGCCA CGCGGATCTC GACATTGATT ATTGACTAGT TATTAATAGT
      601 AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA
      661 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA
      721 CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT
      781 TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT CCGCCCCCTA
      841 TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTACGGG
      901 ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GGTCGAGGTG
      961 AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT
     1021 TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG GGGGGGGGGG GGCGCGCGCC
     1081 AGGCGGGGCG GGGCGGGGCG AGGGGCGGGG CGGGGCGAGG CGGAGAGGTG CGGCGGCAGC
     1141 CAATCAGAGC GGCGCGCTCC GAAAGTTTCC TTTTATGGCG AGGCGGCGGC GGCGGCGGCC
     1201 CTATAAAAAG CGAAGCGCGC GGCGGGCGGG AGTCGCTGCG TTGCCTTCGC CCCGTGCCCC
     1261 GCTCCGCGCC GCCTCGCGCC GCCCGCCCCG GCTCTGACTG ACCGCGTTAC TCCCACAGGT
     1321 GAGCGGGCGG GACGGCCCTT CTCCTCCGGG CTGTAATTAG CGCTTGGTTT AATGACGGCT
     1381 CGTTTCTTTT CTGTGGCTGC GTGAAAGCCT TAAAGGGCTC CGGGAGGGCC CTTTGTGCGG
     1441 GGGGGAGCGG CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCC
     1501 CGCGCTGCCC GGCGGCTGTG AGCGCTGCGG GCGCGGCGCG GGGCTTTGTG CGCTCCGCGT
     1561 GTGCGCGAGG GGAGCGCGGC CGGGGGCGGT GCCCCGCGGT GCGGGGGGGC TGCGAGGGGA
     1621 ACAAAGGCTG CGTGCGGGGT GTGTGCGTGG GGGGGTGAGC AGGGGGTGTG GGCGCGGCGG
     1681 TCGGGCTGTA ACCCCCCCCT GCACCCCCCT CCCCGAGTTG CTGAGCACGG CCCGGCTTCG
     1741 GGTGCGGGGC TCCGTGCGGG GCGTGGCGCG GGGCTCGCCG TGCCGGGCGG GGGGTGGCGG
     1801 CAGGTGGGGG TGCCGGGCGG GGCGGGGCCG CCTCGGGCCG GGGAGGGCTC GGGGGAGGGG
     1861 CGCGGCGGCC CCGGAGCGCC GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT
     1921 TATGGTAATC GTGCGAGAGG GCGCAGGGAC TTCCTTTGTC CCAAATCTGG CGGAGCCGAA
     1981 ATCTGGGAGG CGCCGCCGCA CCCCCTCTag cgggcgcggg gcgaagcggt gcggcgccgg
     2041 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     2101 tctccagcct cggggctgtc cgcgggggga cggctgCCTT CGGGGGGGAC GGGGCAGGGC
     2161 GGGGTTCGGC TTCTGGCGTG TGACCGGCGG CTCTAGCCGG AGCGCACGTC GGCAGTCGGC
     2221 TCCCTCGTTG ACCGAATCAC CGACCTCTCT CCCCAGGGGG ATCCACCGGG CTAGCGCCAC
     2281 CATGATGAAA ATGCAGAAGG CAGGTCCCCC AGTACCTGCC TCGCACATAG CACCTGCCCC
     2341 TGTGCAGCCC CCCATGATTC GGCGGGATAT CACCTTCCCA CCTGGCTCTG TTGAAGCCAC
     2401 ACAGCCTGTG TTGAAGCAGA GGAGGCGGCT CACAATGAAA GACATTGGAA CCCCGGAGGC
     2461 ATGGCGGGTA ATGATGTCCC TCAAGTCTGG TCTCCTGGCA GAGAGCACAT GGGCATTAGA
     2521 TACCATCAAC ATCCTGCTGT ATGATGACAA CAGCATCATG ACCTTCAACC TCAGTCAGCT
     2581 CCCAGGGTTG CTAGAGCTCC TTGTAGAATA TTTCCGACGA TGCCTGATTG AGATCTTTGG
     2641 CATTTTAAAG GAGTATGAGG TGGGTGACCC AGGACAGAGA ACGCTACTGG ATCCTGGGAG
     2701 GTTCAGCAAG GTGTCTAGTC CAGCTCCCAT GGAGGGTGGG GAAGAAGAAG AAGAACTTCT
     2761 AGGTCCTAAA CTAGAAGAGG AAGAAGAAGA GGAAGTAGTT GAAAATGATG AGGAGATAGC
     2821 CTTTTCAGGC AAGGACAAGC CAGCTTCAGA GAATAGTGAG GAGAAGCTGA TCAGTAAGTT
     2881 TGACAAGCTT CCAGTAAAGA TCGTACAGAA GAATGATCCA TTTGTGGTGG ACTGCTCAGA
     2941 TAAGCTTGGG CGTGTGCAGG AGTTTGACAG TGGCCTGCTG CACTGGCGGA TTGGTGGGGG
     3001 GGACACCACT GAGCATATCC AGACCCACTT CGAGAGCAAG ACAGAGCTGC TGCCTTCCCG
     3061 GCCTCACGCA CCCTGCCCAC CAGCCCCTCG GAAGCATGTG ACAACAGCAG AGGGTACACC
     3121 AGGGACAACA GACCAGGAGG GGCCCCCACC TGATGGACCT CCAGAAAAAC GGATCACAGC
     3181 CACTATGGAT GACATGTTGT CTACTCGGTC TAGCACCTTG ACCGAGGATG GAGCTAAGAG
     3241 TTCAGAGGCC ATCAAGGAGA GCAGCAAGTT TCCATTTGGC ATTAGCCCAG CACAGAGCCA
     3301 CCGGAACATC AAGATCCTAG AGGACGAACC CCACAGTAAG GATGAGACCC CACTGTGTAC
     3361 CCTTCTGGAC TGGCAGGATT CTCTTGCCAA GCGCTGCGTC TGTGTGTCCA ATACCATTCG
     3421 AAGCCTGTCA TTTGTGCCAG GCAATGACTT TGAGATGTCC AAACACCCAG GGCTGCTGCT
     3481 CATCCTGGGC AAGCTGATCC TGCTGCACCA CAAGCACCCA GAACGGAAGC AGGCACCACT
     3541 AACTTATGAA AAGGAGGAGG AACAGGACCA AGGGGTGAGC TGCAACAAAG TGGAGTGGTG
     3601 GTGGGACTGC TTGGAGATGC TCCGGGAAAA CACCTTGGTT ACACTCGCCA ACATCTCGGG
     3661 GCAGTTGGAC CTATCTCCAT ACCCCGAGAG CATTTGCCTG CCTGTCCTGG ACGGACTCCT
     3721 ACACTGGGCA GTTTGCCCTT CAGCTGAAGC CCAGGACCCC TTTTCCACCC TGGGCCCCAA
     3781 TGCCGTCCTT TCCCCGCAGA GACTGGTCTT GGAAACCCTC AGCAAACTCA GCATCCAGGA
     3841 CAACAATGTG GACCTGATTC TGGCCACACC CCCCTTCAGC CGCCTGGAGA AGTTGTATAG
     3901 CACTATGGTG CGCTTCCTCA GTGACCGAAA GAACCCGGTG TGCCGGGAGA TGGCTGTGGT
     3961 ACTGCTGGCC AACCTGGCTC AGGGGGACAG CCTGGCAGCT CGTGCCATTG CAGTGCAGAA
     4021 GGGCAGTATC GGCAACCTCC TGGGCTTCCT AGAGGACAGC CTTGCCGCCA CACAGTTCCA
     4081 GCAGAGCCAG GCCAGCCTCC TCCACATGCA GAACCCACCC TTTGAGCCAA CTAGTGTGGA
     4141 CATGATGCGG CGGGCTGCCC GCGCGCTGCT TGCCTTGGCC AAGGTGGACG AGAACCACTC
     4201 AGAGTTTACT CTGTACGAAT CACGGCTGTT GGACATCTCG GTATCACCGC TGATGAATAG
     4261 CCTCGTGTCA CAAGTCATTT GTGATGTACT GTTTTTGATT GGCCAGTCCA CCGGTGACTA
     4321 CAAAGACCAT GACGGTGATT ATAAAGATCA TGATATCGAT TACAAGGATG ACGATGACAA
     4381 GTGAACGCGT TAGTGCGGAT CCTAGTGCGA ATTCTAGTGC GTCGACTCTA Gcaacggttt
     4441 ccctctagcg ggatcaattc cgcccccccc ccctaacgtt actggccgaa gccgcttgga
     4501 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     4561 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     4621 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     4681 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     4741 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     4801 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     4861 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     4921 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     4981 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg gtcatgaaaa
     5041 catttaacat ttctcaacaa gatctagaat tagtagaagt agcgacagag aagattacaa
     5101 tgctttatga ggataataaa catcatgtgg gagcggcaat tcgtacgaaa acaggagaaa
     5161 tcatttcggc agtacatatt gaagcgtata taggacgagt aactgtttgt gcagaagcca
     5221 ttgcgattgg tagtgcagtt tcgaatggac aaaaggattt tgacacgatt gtagctgtta
     5281 gacaccctta ttctgacgaa gtagatagaa gtattcgagt ggtaagtcct tgtggtatgt
     5341 gtagggagtt gatttcagac tatgcaccag attgttttgt gttaatagaa atgaatggca
     5401 agttagtcaa aactacgatt gaagaactca ttccactcaa atatacccga aattaaaagt
     5461 cgagatgcat cgatgatcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     5521 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5581 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5641 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5701 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaacctgca gcccaagctt
     5761 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5821 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5881 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     5941 gatcttaaaa gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     6001 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     6061 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     6121 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     6181 gggttaatct agtatCGTAg Attaattaat ccagcttttg ttccctttag tgagggttaa
     6241 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6301 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6361 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6421 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6481 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6541 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6601 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6661 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6721 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6781 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6841 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6901 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6961 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7021 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7081 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7141 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7201 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7261 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7321 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7381 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7441 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7501 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7561 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7621 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7681 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7741 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7801 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7861 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7921 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7981 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8041 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8101 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8161 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8221 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8281 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8341 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8401 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     8461 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     8521 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     8581 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     8641 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     8701 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     8761 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     8821 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
//