LOCUS       570_pPBbsr2-CAG-ARID1A- 9467 bp ds-DNA     circular     17-JUL-2024
DEFINITION  .
KEYWORDS    "creator:GenScript" "marker:AmpR"
FEATURES             Location/Qualifiers
     primer_bind     142..158
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     promoter        168..186
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     enhancer        571..950
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
     promoter        953..1228
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     source          2009..2136
                     /label="source"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     regulatory      2276..2284
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) regulatory_class: other: "
     misc_feature    2282..2284
                     /label="IDR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2282..4906
                     /label="ARID1A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2282..4981
                     /label="ARID1A"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGDVKLTPATKMNNKADGTPKTESKSKKSSSSTTTNEKITKLYELGGEPERKMWVDRYLAFTEEKAMGMTNLPAVGRKPLDLYRLYVSVKEIGGLTQVNKNKKWRELATNLNVGTSSSAASSLKKQYIQCLYAFECKIERGEDPPPDIFAAADSKKSQPKIQPPSPAGSGSMQGPQTPQSTSSSMAEGGDLKPPTPASTPMKMQKAGPPVPASHIAPAPVQPPMIRRDITFPPGSVEATQPVLKQRRRLTMKDIGTPEAWRVMMSLKSGLLAESTWALDTINILLYDDNSIMTFNLSQLPGLLELLVEYFRRCLIEIFGILKEYEVGDPGQRTLLDPGRFSKVSSPAPMEGGEEEEELLGPKLEEEEEEEVVENDEEIAFSGKDKPASENSEEKLISKFDKLPVKIVQKNDPFVVDCSDKLGRVQEFDSGLLHWRIGGGDTTEHIQTHFESKTELLPSRPHAPCPPAPRKHVTTAEGTPGTTDQEGPPPDGPPEKRITATMDDMLSTRSSTLTEDGAKSSEAIKESSKFPFGISPAQSHRNIKILEDEPHSKDETPLCTLLDWQDSLAKRCVCVSNTIRSLSFVPGNDFEMSKHPGLLLILGKLILLHHKHPERKQAPLTYEKEEEQDQGVSCNKVEWWWDCLEMLRENTLVTLANISGQLDLSPYPESICLPVLDGLLHWAVCPSAEAQDPFSTLGPNAVLSPQRLVLETLSKLSIQDNNVDLILATPPFSRLEKLYSTMVRFLSDRKNPVCREMAVVLLANLAQGDSLAARAIAVQKGSIGNLLGFLEDSLAATQFQQSQASLLHMQNPPFEPTSVDMMRRAARALLALAKVDENHSEFTLYESRLLDISVSPLMNSLVSQVICDVLFLIGQSTGDYKDHDGDYKDHDIDYKDDDDK*"
     misc_feature    2285..2881
                     /label="ARID"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2882..4906
                     /label="CBR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4913..4978
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site transl_table: 1 translation: DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5073..5622
                     /label="IRES"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     polyA_signal    6099..6323
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: bovine growth hormone polyadenylation signal"
     primer_bind     complement(6370..6386)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    6394..6410
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6418..6424)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(6425..6442)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(6443..6448)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    6463..6484
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        complement(6821..6839)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6860..6876)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    6884..6900
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6908..6914)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(6915..6932)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(6933..6938)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    6953..6974
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     rep_origin      complement(7262..7850)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(8021..8812)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(8813..8881)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(8882..8986)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     rep_origin      complement(9012..9467)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      181 tatagggcga attggggcgc gccattctag attaacccta gaaagatagt ctgcgtaaaa
      241 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
      301 tgcatttaGG ACATCTCAGT CGCCGcttgg agctcccgtg aggcgtgctt gtcaatgcgg
      361 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
      421 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
      481 tacgtgataa cttattatat atatattttc ttgttataga tatcaactag aatgCTAGAC
      541 TCGAGAAGCT TGATCGGCCA CGCGGATCTC GACATTGATT ATTGACTAGT TATTAATAGT
      601 AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA
      661 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA
      721 CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT
      781 TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT CCGCCCCCTA
      841 TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTACGGG
      901 ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GGTCGAGGTG
      961 AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT
     1021 TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG GGGGGGGGGG GGCGCGCGCC
     1081 AGGCGGGGCG GGGCGGGGCG AGGGGCGGGG CGGGGCGAGG CGGAGAGGTG CGGCGGCAGC
     1141 CAATCAGAGC GGCGCGCTCC GAAAGTTTCC TTTTATGGCG AGGCGGCGGC GGCGGCGGCC
     1201 CTATAAAAAG CGAAGCGCGC GGCGGGCGGG AGTCGCTGCG TTGCCTTCGC CCCGTGCCCC
     1261 GCTCCGCGCC GCCTCGCGCC GCCCGCCCCG GCTCTGACTG ACCGCGTTAC TCCCACAGGT
     1321 GAGCGGGCGG GACGGCCCTT CTCCTCCGGG CTGTAATTAG CGCTTGGTTT AATGACGGCT
     1381 CGTTTCTTTT CTGTGGCTGC GTGAAAGCCT TAAAGGGCTC CGGGAGGGCC CTTTGTGCGG
     1441 GGGGGAGCGG CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCC
     1501 CGCGCTGCCC GGCGGCTGTG AGCGCTGCGG GCGCGGCGCG GGGCTTTGTG CGCTCCGCGT
     1561 GTGCGCGAGG GGAGCGCGGC CGGGGGCGGT GCCCCGCGGT GCGGGGGGGC TGCGAGGGGA
     1621 ACAAAGGCTG CGTGCGGGGT GTGTGCGTGG GGGGGTGAGC AGGGGGTGTG GGCGCGGCGG
     1681 TCGGGCTGTA ACCCCCCCCT GCACCCCCCT CCCCGAGTTG CTGAGCACGG CCCGGCTTCG
     1741 GGTGCGGGGC TCCGTGCGGG GCGTGGCGCG GGGCTCGCCG TGCCGGGCGG GGGGTGGCGG
     1801 CAGGTGGGGG TGCCGGGCGG GGCGGGGCCG CCTCGGGCCG GGGAGGGCTC GGGGGAGGGG
     1861 CGCGGCGGCC CCGGAGCGCC GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT
     1921 TATGGTAATC GTGCGAGAGG GCGCAGGGAC TTCCTTTGTC CCAAATCTGG CGGAGCCGAA
     1981 ATCTGGGAGG CGCCGCCGCA CCCCCTCTag cgggcgcggg gcgaagcggt gcggcgccgg
     2041 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     2101 tctccagcct cggggctgtc cgcgggggga cggctgCCTT CGGGGGGGAC GGGGCAGGGC
     2161 GGGGTTCGGC TTCTGGCGTG TGACCGGCGG CTCTAGCCGG AGCGCACGTC GGCAGTCGGC
     2221 TCCCTCGTTG ACCGAATCAC CGACCTCTCT CCCCAGGGGG ATCCACCGGG CTAGCGCCAC
     2281 CATGGGGGAT GTAAAGTTAA CTCCAGCCAC CAAAATGAAC AACAAGGCAG ATGGGACACC
     2341 CAAGACAGAA TCCAAATCCA AGAAATCCAG TTCTTCTACT ACAACCAATG AGAAGATCAC
     2401 CAAGTTGTAT GAGCTGGGTG GTGAGCCTGA GAGGAAGATG TGGGTGGACC GTTATCTGGC
     2461 CTTCACTGAG GAGAAGGCCA TGGGCATGAC AAATCTGCCT GCTGTGGGTA GGAAACCTCT
     2521 GGACCTCTAT CGCCTCTATG TGTCTGTGAA GGAGATTGGT GGATTGACTC AGGTCAACAA
     2581 GAACAAAAAA TGGCGGGAAC TTGCAACCAA CCTCAATGTG GGCACATCAA GCAGTGCTGC
     2641 CAGCTCCTTG AAAAAGCAGT ATATCCAGTG TCTCTATGCC TTTGAATGCA AGATTGAACG
     2701 GGGAGAAGAC CCTCCCCCAG ACATCTTTGC AGCTGCTGAT TCCAAGAAGT CCCAGCCCAA
     2761 GATCCAGCCT CCCTCTCCTG CGGGATCAGG ATCTATGCAG GGGCCCCAGA CTCCCCAGTC
     2821 AACCAGCAGT TCCATGGCAG AAGGAGGAGA CTTAAAGCCA CCAACTCCAG CATCCACACC
     2881 AATGAAAATG CAGAAGGCAG GTCCCCCAGT ACCTGCCTCG CACATAGCAC CTGCCCCTGT
     2941 GCAGCCCCCC ATGATTCGGC GGGATATCAC CTTCCCACCT GGCTCTGTTG AAGCCACACA
     3001 GCCTGTGTTG AAGCAGAGGA GGCGGCTCAC AATGAAAGAC ATTGGAACCC CGGAGGCATG
     3061 GCGGGTAATG ATGTCCCTCA AGTCTGGTCT CCTGGCAGAG AGCACATGGG CATTAGATAC
     3121 CATCAACATC CTGCTGTATG ATGACAACAG CATCATGACC TTCAACCTCA GTCAGCTCCC
     3181 AGGGTTGCTA GAGCTCCTTG TAGAATATTT CCGACGATGC CTGATTGAGA TCTTTGGCAT
     3241 TTTAAAGGAG TATGAGGTGG GTGACCCAGG ACAGAGAACG CTACTGGATC CTGGGAGGTT
     3301 CAGCAAGGTG TCTAGTCCAG CTCCCATGGA GGGTGGGGAA GAAGAAGAAG AACTTCTAGG
     3361 TCCTAAACTA GAAGAGGAAG AAGAAGAGGA AGTAGTTGAA AATGATGAGG AGATAGCCTT
     3421 TTCAGGCAAG GACAAGCCAG CTTCAGAGAA TAGTGAGGAG AAGCTGATCA GTAAGTTTGA
     3481 CAAGCTTCCA GTAAAGATCG TACAGAAGAA TGATCCATTT GTGGTGGACT GCTCAGATAA
     3541 GCTTGGGCGT GTGCAGGAGT TTGACAGTGG CCTGCTGCAC TGGCGGATTG GTGGGGGGGA
     3601 CACCACTGAG CATATCCAGA CCCACTTCGA GAGCAAGACA GAGCTGCTGC CTTCCCGGCC
     3661 TCACGCACCC TGCCCACCAG CCCCTCGGAA GCATGTGACA ACAGCAGAGG GTACACCAGG
     3721 GACAACAGAC CAGGAGGGGC CCCCACCTGA TGGACCTCCA GAAAAACGGA TCACAGCCAC
     3781 TATGGATGAC ATGTTGTCTA CTCGGTCTAG CACCTTGACC GAGGATGGAG CTAAGAGTTC
     3841 AGAGGCCATC AAGGAGAGCA GCAAGTTTCC ATTTGGCATT AGCCCAGCAC AGAGCCACCG
     3901 GAACATCAAG ATCCTAGAGG ACGAACCCCA CAGTAAGGAT GAGACCCCAC TGTGTACCCT
     3961 TCTGGACTGG CAGGATTCTC TTGCCAAGCG CTGCGTCTGT GTGTCCAATA CCATTCGAAG
     4021 CCTGTCATTT GTGCCAGGCA ATGACTTTGA GATGTCCAAA CACCCAGGGC TGCTGCTCAT
     4081 CCTGGGCAAG CTGATCCTGC TGCACCACAA GCACCCAGAA CGGAAGCAGG CACCACTAAC
     4141 TTATGAAAAG GAGGAGGAAC AGGACCAAGG GGTGAGCTGC AACAAAGTGG AGTGGTGGTG
     4201 GGACTGCTTG GAGATGCTCC GGGAAAACAC CTTGGTTACA CTCGCCAACA TCTCGGGGCA
     4261 GTTGGACCTA TCTCCATACC CCGAGAGCAT TTGCCTGCCT GTCCTGGACG GACTCCTACA
     4321 CTGGGCAGTT TGCCCTTCAG CTGAAGCCCA GGACCCCTTT TCCACCCTGG GCCCCAATGC
     4381 CGTCCTTTCC CCGCAGAGAC TGGTCTTGGA AACCCTCAGC AAACTCAGCA TCCAGGACAA
     4441 CAATGTGGAC CTGATTCTGG CCACACCCCC CTTCAGCCGC CTGGAGAAGT TGTATAGCAC
     4501 TATGGTGCGC TTCCTCAGTG ACCGAAAGAA CCCGGTGTGC CGGGAGATGG CTGTGGTACT
     4561 GCTGGCCAAC CTGGCTCAGG GGGACAGCCT GGCAGCTCGT GCCATTGCAG TGCAGAAGGG
     4621 CAGTATCGGC AACCTCCTGG GCTTCCTAGA GGACAGCCTT GCCGCCACAC AGTTCCAGCA
     4681 GAGCCAGGCC AGCCTCCTCC ACATGCAGAA CCCACCCTTT GAGCCAACTA GTGTGGACAT
     4741 GATGCGGCGG GCTGCCCGCG CGCTGCTTGC CTTGGCCAAG GTGGACGAGA ACCACTCAGA
     4801 GTTTACTCTG TACGAATCAC GGCTGTTGGA CATCTCGGTA TCACCGCTGA TGAATAGCCT
     4861 CGTGTCACAA GTCATTTGTG ATGTACTGTT TTTGATTGGC CAGTCCACCG GTGACTACAA
     4921 AGACCATGAC GGTGATTATA AAGATCATGA TATCGATTAC AAGGATGACG ATGACAAGTG
     4981 AACGCGTTAG TGCGGATCCT AGTGCGAATT CTAGTGCGTC GACTCTAGca acggtttccc
     5041 tctagcggga tcaattccgc cccccccccc taacgttact ggccgaagcc gcttggaata
     5101 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     5161 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     5221 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     5281 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     5341 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     5401 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     5461 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     5521 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     5581 gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatggtc atgaaaacat
     5641 ttaacatttc tcaacaagat ctagaattag tagaagtagc gacagagaag attacaatgc
     5701 tttatgagga taataaacat catgtgggag cggcaattcg tacgaaaaca ggagaaatca
     5761 tttcggcagt acatattgaa gcgtatatag gacgagtaac tgtttgtgca gaagccattg
     5821 cgattggtag tgcagtttcg aatggacaaa aggattttga cacgattgta gctgttagac
     5881 acccttattc tgacgaagta gatagaagta ttcgagtggt aagtccttgt ggtatgtgta
     5941 gggagttgat ttcagactat gcaccagatt gttttgtgtt aatagaaatg aatggcaagt
     6001 tagtcaaaac tacgattgaa gaactcattc cactcaaata tacccgaaat taaaagtcga
     6061 gatgcatcga tgatctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     6121 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6181 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6241 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6301 tggggatgcg gtgggctcta tggcttctga ggcggaaaga acctgcagcc caagcttggc
     6361 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6421 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6481 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
     6541 cttaaaagtt ttgttacttt atagaagaaa ttttgagttt ttgttttttt ttaataaata
     6601 aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata
     6661 aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat
     6721 gcgtcaattt tacgcatgat tatctttaac gtacgtcaca atatgattat ctttctaggg
     6781 ttaatctagt atCGTAgAtt aattaatcca gcttttgttc cctttagtga gggttaattg
     6841 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6901 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6961 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7021 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7081 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7141 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7201 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7261 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7321 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7381 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7441 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7501 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7561 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7621 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7681 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7741 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7801 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7861 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7921 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7981 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8041 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8101 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8161 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8221 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8281 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8341 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8401 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8461 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8521 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8581 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8641 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8701 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8761 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8821 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8881 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8941 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9001 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     9061 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     9121 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     9181 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     9241 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     9301 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     9361 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     9421 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgt
//