LOCUS       512_pPBbsr2-CAG-NR5A1-3 8234 bp ds-DNA     circular     17-OCT-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        1813..2116
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     pro             2117..3365
                     /label="beta-actin promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     ORF             3454..4836
                     /label="SF1 ORF"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             3454..4914
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCTESQSCKIDKTQRKRCPFCRFQKCLTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKAQIRANGFKLETGPPMGVPPPPPPAPDYVLPPSLHGPEPKGLAAGPPAGPLGDFGAPALPMAVPGAHGPLAGYLYPAFPGRAIKSEYPEPYASPPQPGLPYGYPEPFSGGPNVPELILQLLQLEPDEDQVRARILGCLQEPTKSRPDQPAAFGLLCRMADQTFISIVDWARRCMVFKELEVADQMTLLQNCWSELLVFDHIYRQVQHGKEGSILLVTGQEVELTTVATQAGSLLHSLVLRAQELVLQLLALQLDRQEFVCLKFIILFSLDLKFLNNHILVKDAQEKANAALLDYTLCHYPHCGDKFQQLLLCLVEVRALSMQAKEYLYHKHLGNEMPRNNLLIEMLQAKQTTGMDYKDHDGDYKDHDIDYKDDDDK*"
     misc_feature    3490..3768
                     /label="DBD"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3862..4101
                     /label="PHA03247"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4120..4830
                     /label="LBD"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4843..4911
                     /label="3x Flag"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        complement(6351..6381)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(6754..6772)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        complement(6841..6871)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(7957..382)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT
       61 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC
      121 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT
      181 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC
      241 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT
      301 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA
      361 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA
      421 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG
      481 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA
      541 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT
      601 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA
      661 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG
      721 CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA
      781 ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG
      841 TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG
      901 GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT
      961 TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG
     1021 AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC
     1081 GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC
     1141 GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA
     1201 AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC
     1261 AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC
     1321 CAGTGAGCGC GCGTAATACG ACTCACTATA GGGCGAATTG GGGCGCGCCA TTCTAGATTA
     1381 ACCCTAGAAA GATAGTCTGC GTAAAATTGA CGCATGCATT CTTGAAATAT TGCTCTCTCT
     1441 TTCTAAATAG CGCGAATCCG TCGCTGTGCA TTTAGGACAT CTCAGTCGCC GCTTGGAGCT
     1501 CCCGTGAGGC GTGCTTGTCA ATGCGGTAAG TGTCACTGAT TTTGAACTAT AACGACCGCG
     1561 TGAGTCAAAA TGACGCATGA TTATCTTTTA CGTGACTTTT AAGATTTAAC TCATACGATA
     1621 ATTATATTGT TATTTCATGT TCTACTTACG TGATAACTTA TTATATATAT ATTTTCTTGT
     1681 TATAGATATC AACTAGAATG CTAGACTCGA GAAGCTTGAT CGGCCACGCG GATCTCGACA
     1741 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA
     1801 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA
     1861 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT
     1921 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT
     1981 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA
     2041 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT
     2101 CATCGCTATT ACCATGGTCG AGGTGAGCCC CACGTTCTGC TTCACTCTCC CCATCTCCCC
     2161 CCCCTCCCCA CCCCCAATTT TGTATTTATT TATTTTTTAA TTATTTTGTG CAGCGATGGG
     2221 GGCGGGGGGG GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG GCGGGGCGGG
     2281 GCGAGGCGGA GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA GTTTCCTTTT
     2341 ATGGCGAGGC GGCGGCGGCG GCGGCCCTAT AAAAAGCGAA GCGCGCGGCG GGCGGGAGTC
     2401 GCTGCGCGCT GCCTTCGCCC CGTGCCCCGC TCCGCCGCCG CCTCGCGCCG CCCGCCCCGG
     2461 CTCTGACTGA CCGCGTTACT CCCACAGGTG AGCGGGCGGG ACGGCCCTTC TCCTCCGGGC
     2521 TGTAATTAGC GCTTGGTTTA ATGACGGCTT GTTTCTTTTC TGTGGCTGCG TGAAAGCCTT
     2581 GAGGGGCTCC GGGAGGGCCC TTTGTGCGGG GGGAGCGGCT CGGGGGGTGC GTGCGTGTGT
     2641 GTGTGCGTGG GGAGCGCCGC GTGCGGCTCC GCGCTGCCCG GCGGCTGTGA GCGCTGCGGG
     2701 CGCGGCGCGG GGCTTTGTGC GCTCCGCAGT GTGCGCGAGG GGAGCGCGGC CGGGGGCGGT
     2761 GCCCCGCGGT GCGGGGGGGG CTGCGAGGGG AACAAAGGCT GCGTGCGGGG TGTGTGCGTG
     2821 GGGGGGTGAG CAGGGGGTGT GGGCGCGTCG GTCGGGCTGC AACCCCCCCT GCACCCCCCT
     2881 CCCCGAGTTG CTGAGCACGG CCCGGCTTCG GGTGCGGGGC TCCGTACGGG GCGTGGCGCG
     2941 GGGCTCGCCG TGCCGGGCGG GGGGTGGCGG CAGGTGGGGG TGCCGGGCGG GGCGGGGCCG
     3001 CCTCGGGCCG GGGAGGGCTC GGGGGAGGGG CGCGGCGGCC CCCGGAGCGC CGGCGGCTGT
     3061 CGAGGCGCGG CGAGCCGCAG CCATTGCCTT TTATGGTAAT CGTGCGAGAG GGCGCAGGGA
     3121 CTTCCTTTGT CCCAAATCTG TGCGGAGCCG AAATCTGGGA GGCGCCGCCG CACCCCCTCT
     3181 AGCGGGCGCG GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT
     3241 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CCTCTCCAGC CTCGGGGCTG TCCGCGGGGG
     3301 GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC
     3361 GGCTCTAGCC GGAGCGCACG TCGGCAGTCG GCTCCCTCGT TGACCGAATC ACCGACCTCT
     3421 CTCCCCAGGG GGATCCACCG GGCTAGCGCC ACCATGGACT ATTCGTACGA CGAGGACCTG
     3481 GACGAGCTGT GCCCCGTGTG CGGGGACAAG GTGTCCGGCT ACCACTACGG ACTGCTCACG
     3541 TGTGAGAGCT GCAAGGGCTT CTTCAAGCGC ACGGTGCAGA ACAACAAGCA CTACACGTGC
     3601 ACCGAGAGCC AGAGCTGCAA GATCGACAAG ACGCAGCGCA AGCGCTGTCC CTTCTGCCGC
     3661 TTCCAGAAAT GCCTGACGGT GGGGATGCGC CTGGAAGCCG TGCGCGCTGA CCGTATGAGG
     3721 GGTGGCCGGA ACAAGTTTGG GCCGATGTAC AAGCGGGACC GGGCCCTGAA ACAGCAGAAG
     3781 AAGGCACAGA TTCGGGCCAA TGGCTTCAAG CTGGAGACAG GGCCCCCGAT GGGGGTGCCC
     3841 CCGCCGCCCC CTCCCGCACC GGACTACGTG CTGCCTCCCA GCCTGCATGG GCCTGAGCCC
     3901 AAGGGCCTGG CCGCCGGTCC ACCTGCTGGG CCACTGGGCG ACTTTGGGGC CCCAGCACTG
     3961 CCCATGGCCG TGCCCGGTGC CCACGGGCCA CTGGCTGGCT ACCTCTACCC TGCCTTTCCT
     4021 GGCCGTGCCA TCAAGTCTGA GTACCCGGAG CCTTATGCCA GCCCCCCACA GCCTGGGCTG
     4081 CCGTACGGCT ACCCAGAGCC CTTCTCTGGA GGGCCCAACG TGCCTGAGCT CATCCTGCAG
     4141 CTGCTGCAGC TGGAGCCGGA TGAGGACCAG GTGCGGGCCC GCATCTTGGG CTGCCTGCAG
     4201 GAGCCCACCA AAAGCCGCCC CGACCAGCCG GCGGCCTTCG GCCTCCTGTG CAGAATGGCC
     4261 GACCAGACCT TCATCTCCAT CGTGGACTGG GCACGCAGGT GCATGGTCTT CAAGGAGCTG
     4321 GAGGTGGCCG ACCAGATGAC GCTGCTGCAG AACTGCTGGA GCGAGCTGCT GGTGTTCGAC
     4381 CACATCTACC GCCAGGTCCA GCACGGCAAG GAGGGCAGCA TCCTGCTGGT CACCGGGCAG
     4441 GAGGTGGAGC TGACCACAGT GGCCACCCAG GCGGGCTCGC TGCTGCACAG CCTGGTGTTG
     4501 CGGGCGCAGG AGCTGGTGCT GCAGCTGCTT GCGCTGCAGC TGGACCGGCA GGAGTTTGTC
     4561 TGCCTCAAGT TCATCATCCT CTTCAGCCTG GATTTGAAGT TCCTGAATAA CCACATCCTG
     4621 GTGAAAGACG CTCAGGAGAA GGCCAACGCC GCCCTGCTTG ACTACACCCT GTGCCACTAC
     4681 CCGCACTGCG GGGACAAATT CCAGCAGCTG CTGCTGTGCC TGGTGGAGGT GCGGGCCCTG
     4741 AGCATGCAGG CCAAGGAGTA CCTGTACCAC AAGCACCTGG GCAACGAGAT GCCCCGCAAC
     4801 AACCTGCTCA TCGAAATGCT GCAAGCCAAG CAGACTACCG GTATGGACTA CAAAGACCAT
     4861 GACGGTGATT ATAAAGATCA TGATATCGAT TACAAGGATG ACGATGACAA GTGAACGCGT
     4921 TAGTGCGGAT CCTAGTGCGA ATTCTAGTGC GTCGACTCTA GCAACGGTTT CCCTCTAGCG
     4981 GGATCAATTC CGCCCCCCCC CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG
     5041 TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC
     5101 CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA
     5161 GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA
     5221 CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC
     5281 CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC
     5341 CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC TCTCCTCAAG CGTATTCAAC
     5401 AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG
     5461 TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC
     5521 GGGGACGTGG TTTTCCTTTG AAAAACACGA TAATACCATG GTCATGAAAA CATTTAACAT
     5581 TTCTCAACAA GATCTAGAAT TAGTAGAAGT AGCGACAGAG AAGATTACAA TGCTTTATGA
     5641 GGATAATAAA CATCATGTGG GAGCGGCAAT TCGTACGAAA ACAGGAGAAA TCATTTCGGC
     5701 AGTACATATT GAAGCGTATA TAGGACGAGT AACTGTTTGT GCAGAAGCCA TTGCGATTGG
     5761 TAGTGCAGTT TCGAATGGAC AAAAGGATTT TGACACGATT GTAGCTGTTA GACACCCTTA
     5821 TTCTGACGAA GTAGATAGAA GTATTCGAGT GGTAAGTCCT TGTGGTATGT GTAGGGAGTT
     5881 GATTTCAGAC TATGCACCAG ATTGTTTTGT GTTAATAGAA ATGAATGGCA AGTTAGTCAA
     5941 AACTACGATT GAAGAACTCA TTCCACTCAA ATATACCCGA AATTAAAAGT CGAGATGCAT
     6001 CGATGATCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT
     6061 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC
     6121 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT
     6181 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT
     6241 GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCTGCA GCCCAAGCTT GGCGTAATCA
     6301 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA
     6361 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT
     6421 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCG GATCTTAAAA
     6481 GTTTTGTTAC TTTATAGAAG AAATTTTGAG TTTTTGTTTT TTTTTAATAA ATAAATAAAC
     6541 ATAAATAAAT TGTTTGTTGA ATTTATTATT AGTATGTAAG TGTAAATATA ATAAAACTTA
     6601 ATATCTATTC AAATTAATAA ATAAACCTCG ATATACAGAC CGATAAAACA CATGCGTCAA
     6661 TTTTACGCAT GATTATCTTT AACGTACGTC ACAATATGAT TATCTTTCTA GGGTTAATCT
     6721 AGTATCGTAG ATTAATTAAT CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT
     6781 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
     6841 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT
     6901 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
     6961 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC
     7021 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
     7081 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
     7141 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
     7201 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     7261 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     7321 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     7381 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     7441 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     7501 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     7561 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     7621 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     7681 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     7741 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
     7801 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
     7861 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
     7921 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
     7981 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
     8041 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC
     8101 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
     8161 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
     8221 AGTAAGTAGT TCGC
//