LOCUS       VB200923-1218avm        6260 bp DNA     circular SYN 25-JUN-2025
DEFINITION  .
ACCESSION   VB200923-1218avm
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     Sequence Label: VB200923-1218avm.
FEATURES             Location/Qualifiers
     source          1..6260
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=AAV2 ITR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/name=AAV2 ITR"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=5f14ec1a645a407dab2725d71bd1c86a"
                     /note="/vntifkey=21"
                     /note="color: #800c0c; direction: RIGHT"
     misc_feature    150..155
                     /label=MluI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=MluI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=157d822145f14df2a23981abab96a158"
                     /note="/vntifkey=21"
                     /note="color: #0c5d90; direction: RIGHT"
     misc_feature    156..624
                     /label=SYN1
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Tissue specificity: Brain. Cell 
                     typespecificity: Mature neurons."
                     /note="/full_name=Human synapsin I promoter"
                     /note="/name=SYN1"
                     /note="/official_designation=SYN1"
                     /note="/uuid=6f036b2e82244653b8e92867f598e473"
                     /note="/vntifkey=21"
                     /note="color: #f58600; direction: RIGHT"
     misc_feature    631..636
                     /label=BamHI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=BamHI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=2e24d26a052f4a8f9539513b7d1f974d"
                     /note="/vntifkey=21"
                     /note="color: #5566f5; direction: RIGHT"
     misc_feature    655..688
                     /label=Fas
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Fas"
                     /note="/official_designation=Fas"
                     /note="/uuid=8c06f01ac8a7409a8154114013657249"
                     /note="/vntifkey=21"
                     /note="color: #ef6cdf; direction: RIGHT"
     misc_feature    689..696
                     /label=AscI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows linearization of vector."
                     /note="/full_name=AscI restriction enzyme site"
                     /note="/name=AscI"
                     /note="/official_designation=AscI"
                     /note="/uuid=abe053952fef456197b891413915b7a2"
                     /note="/vntifkey=21"
                     /note="color: #c54b7c; direction: RIGHT"
     misc_feature    697..702
                     /label=Kozak
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="/vntifkey=21"
                     /note="color: #d05c0a; direction: RIGHT"
     misc_feature    703..1419
                     /label=EGFP
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Commonly used green 
                     fluorescentprotein; ranked high in brightness, 
                     photostability and pH stability among all fluorescent 
                     proteins."
                     /note="/full_name=Enhanced green fluorescent protein(ns)"
                     /note="/name=EGFP"
                     /note="/official_designation=EGFP(ns)"
                     /note="/uuid=5443fd992fa248b69b274cf75346abad"
                     /note="/vntifkey=21"
                     /note="color: #fd3434; direction: RIGHT"
     misc_feature    1426..1491
                     /label=P2A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Causes co-translational cleavage 
                     of theencoded polypeptide. Multiple proteins can be made 
                     from a polycistronic transcript containing multiple ORFs 
                     separated by 2A. P2A and T2A have higher cleavage 
                     efficiency compared to other 2As."
                     /note="/full_name=Porcine teschovirus-1 2A peptide"
                     /note="/name=P2A"
                     /note="/official_designation=P2A"
                     /note="/uuid=a06180cab93f417790ce7ed18e312702"
                     /note="/vntifkey=21"
                     /note="color: #46c6ef; direction: RIGHT"
     misc_feature    1498..2319
                     /label=mNaChBac
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=mNaChBac"
                     /note="/official_designation=mNaChBac"
                     /note="/uuid=36da0b1671af49e0b006f464f3fdb473"
                     /note="/vntifkey=21"
                     /note="color: #eda481; direction: RIGHT"
     misc_feature    2323..2328
                     /label=NheI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=NheI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=efd7b4e467c64c70ae8bfad8c16a27cb"
                     /note="/vntifkey=21"
                     /note="color: #da3bb5; direction: RIGHT"
     misc_feature    2329..2362
                     /label=Fas
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Fas"
                     /note="/official_designation=Fas"
                     /note="/uuid=8c06f01ac8a7409a8154114013657249"
                     /note="/vntifkey=21"
                     /note="color: #e4b930; direction: RIGHT"
     misc_feature    2387..2975
                     /label=WPRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=c0e1734c945b4c6cab315200062119b2"
                     /note="/vntifkey=21"
                     /note="color: #7a00b5; direction: RIGHT"
     misc_feature    3007..3483
                     /label=hGH poly(A) signal
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=hGH poly(A) signal"
                     /note="/official_designation=hGH poly(A) signal"
                     /note="/uuid=a0d4b43c08404c7aa83ae7bcea391038"
                     /note="/vntifkey=21"
                     /note="color: #05696d; direction: RIGHT"
     misc_feature    3523..3663
                     /label=AAV2 ITR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/name=AAV2 ITR"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=5b8101593b634e64aa9eef9434216f71"
                     /note="/vntifkey=21"
                     /note="color: #f54c6b; direction: RIGHT"
     misc_feature    4580..5440
                     /label=AmpR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=AmpR"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
                     /note="/vntifkey=21"
                     /note="color: #944603; direction: RIGHT"
     misc_feature    5611..6199
                     /label=ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates low-copy plasmid number when Rop 
                     protein is presence (15-20) and medium-copy plasmid number 
                     when Rop is absent (100-300)."
                     /note="/full_name=pBR322 origin of replication"
                     /note="/name=ori"
                     /note="/official_designation=pBR322 ori"
                     /note="/uuid=b523c7b87d6149148546f5bcbd4831c3"
                     /note="/vntifkey=21"
                     /note="color: #ce0084; direction: RIGHT"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtctgca gagggccctg cgtatgagtg
      181 caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc
      241 gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg
      301 gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca
      361 gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg
      421 acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc
      481 gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac
      541 catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga
      601 gtcgtgtcgt gcctgagagc gcagggtacc ggatcctcta gagtcgactc cggaacaact
      661 tcgtatatac ctttctatac gaagttgtgg cgcgccgcca ccatggtgag caagggcgag
      721 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      781 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      841 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      901 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      961 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1021 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1081 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1141 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1201 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1261 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1321 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1381 gccgccggga tcactctcgg catggacgag ctgtacaagc tcgagggaag cggagccact
     1441 aacttctccc tgttgaaaca agcaggggat gtcgaagaga atcccgggcc atctagaatg
     1501 aaaatggaag cacgccagaa acagaactca ttcacgtcaa agatgcagaa aattgtcaac
     1561 catagagcgt ttactttcac cgtcattgcc ctgatcctct ttaatgcact gatcgtggga
     1621 atcgaaacat atccgaggat ctacgcggac cacaagtggc tcttttatcg gattgacctc
     1681 gtcctgcttt ggatcttcac catcgagatc gcgatgaggt ttttggcgtc gaacccaaag
     1741 tcggccttct tccgcagctc gtggaattgg tttgattttc tgattgtcgc ggctgggcat
     1801 atcttcgcgg gagcccagtt cgtgacagta ttgcgaatcc ttagagtgct gcgggtactc
     1861 agggcgatct ccgtcgtccc ctccctgcgc agactcgtag atgcgctggt gatgacgatt
     1921 ccggcgttgg gcaatatctt gatcttgatg tccatcttct tctatatctt tgcagtgatc
     1981 gggacaatgc tcttccagca cgtatcgcct gaatactttg ggaacctcca gctttcgttg
     2041 ctgacgctgt tccaagtggt gaccttggag agctgggcca gcggtgtaat gcggcccatt
     2101 tttgcagagg tgccctggtc atggctctac tttgtctcgt ttgtcctgat tggtactttt
     2161 atcattttca atcttttcat cggagtcatt gtgaacaacg tggaaaaggc ggagcttacg
     2221 gacaatgagg aagatgggga ggccgacgga cttaaacagg agatttcagc attgcgaaag
     2281 gatgtagccg aactgaagag cttgctcaaa caacttaagt aagctagcac aacttcgtat
     2341 atacctttct atacgaagtt gtgaattcga tatcaagctt atcgataatc aacctctgga
     2401 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2461 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2521 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2581 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2641 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2701 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2761 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac
     2821 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     2881 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     2941 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag
     3001 atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     3061 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     3121 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     3181 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     3241 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     3301 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     3361 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     3421 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     3481 cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     3541 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     3601 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     3661 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     3721 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3781 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3841 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3901 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     3961 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4021 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     4081 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4141 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4201 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4261 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4321 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4381 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4441 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4501 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4561 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4621 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4681 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4741 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4801 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4861 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4921 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4981 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5041 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5101 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5161 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5221 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5281 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5341 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5401 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5461 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5521 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5581 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5641 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5701 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5761 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5821 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5881 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5941 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6001 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6061 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6121 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6181 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6241 ctggcctttt gctcacatgt
//
