LOCUS       PAAV-SYN1-Fas-My        6731 bp DNA     circular SYN 02-JUL-2025
DEFINITION  .
ACCESSION   VB200923-1219kwr
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     Sequence Label: PAAV-SYN1-Fas-Myc/Kir2.1
            (E224GY242F) P2A.dTomato
FEATURES             Location/Qualifiers
     source          1..6731
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=AAV2 ITR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/name=AAV2 ITR"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=5f14ec1a645a407dab2725d71bd1c86a"
                     /note="/vntifkey=21"
                     /note="color: #a949ca; direction: RIGHT"
     misc_feature    150..155
                     /label=MluI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=MluI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=157d822145f14df2a23981abab96a158"
                     /note="/vntifkey=21"
                     /note="color: #946a00; direction: RIGHT"
     misc_feature    156..624
                     /label=SYN1
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Tissue specificity: Brain. Cell 
                     typespecificity: Mature neurons."
                     /note="/full_name=Human synapsin I promoter"
                     /note="/name=SYN1"
                     /note="/official_designation=SYN1"
                     /note="/uuid=6f036b2e82244653b8e92867f598e473"
                     /note="/vntifkey=21"
                     /note="color: #512bbd; direction: RIGHT"
     misc_feature    631..636
                     /label=BamHI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=BamHI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=2e24d26a052f4a8f9539513b7d1f974d"
                     /note="/vntifkey=21"
                     /note="color: #d84e4e; direction: RIGHT"
     misc_feature    655..688
                     /label=Fas
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Fas"
                     /note="/official_designation=Fas"
                     /note="/uuid=8c06f01ac8a7409a8154114013657249"
                     /note="/vntifkey=21"
                     /note="color: #1f36a9; direction: RIGHT"
     misc_feature    689..696
                     /label=AscI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows linearization of vector."
                     /note="/full_name=AscI restriction enzyme site"
                     /note="/name=AscI"
                     /note="/official_designation=AscI"
                     /note="/uuid=abe053952fef456197b891413915b7a2"
                     /note="/vntifkey=21"
                     /note="color: #db6901; direction: RIGHT"
     misc_feature    697..702
                     /label=Kozak
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="/vntifkey=21"
                     /note="color: #800c0c; direction: RIGHT"
     misc_feature    703..2016
                     /label=Myc/Kir2.1*(E224G Y242F)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Myc/Kir2.1*(E224G Y242F)"
                     /note="/official_designation=Myc/Kir2.1*(E224G Y242F)"
                     /note="/uuid=54d966e7330c4199b05f557bb303a0a3"
                     /note="/vntifkey=21"
                     /note="color: #0c5d90; direction: RIGHT"
     misc_feature    2023..2088
                     /label=P2A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Causes co-translational cleavage 
                     of theencoded polypeptide. Multiple proteins can be made 
                     from a polycistronic transcript containing multiple ORFs 
                     separated by 2A. P2A and T2A have higher cleavage 
                     efficiency compared to other 2As."
                     /note="/full_name=Porcine teschovirus-1 2A peptide"
                     /note="/name=P2A"
                     /note="/official_designation=P2A"
                     /note="/uuid=a06180cab93f417790ce7ed18e312702"
                     /note="/vntifkey=21"
                     /note="color: #f58600; direction: RIGHT"
     misc_feature    2089..2793
                     /label=dTomato
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Fast maturation but slightly 
                     lowerbrightness compared to its predecessor DsRed."
                     /note="/full_name=dTomato"
                     /note="/name=dTomato"
                     /note="/official_designation=dTomato"
                     /note="/uuid=47065b8894d149d0a817e5566ac51ed5"
                     /note="/vntifkey=21"
                     /note="color: #5566f5; direction: RIGHT"
     misc_feature    2794..2799
                     /label=NheI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=NheI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=efd7b4e467c64c70ae8bfad8c16a27cb"
                     /note="/vntifkey=21"
                     /note="color: #ef6cdf; direction: RIGHT"
     misc_feature    2800..2833
                     /label=Fas
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Fas"
                     /note="/official_designation=Fas"
                     /note="/uuid=8c06f01ac8a7409a8154114013657249"
                     /note="/vntifkey=21"
                     /note="color: #c54b7c; direction: RIGHT"
     misc_feature    2858..3446
                     /label=WPRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=c0e1734c945b4c6cab315200062119b2"
                     /note="/vntifkey=21"
                     /note="color: #d05c0a; direction: RIGHT"
     misc_feature    3478..3954
                     /label=hGH poly(A) signal
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=hGH poly(A) signal"
                     /note="/official_designation=hGH poly(A) signal"
                     /note="/uuid=a0d4b43c08404c7aa83ae7bcea391038"
                     /note="/vntifkey=21"
                     /note="color: #fd3434; direction: RIGHT"
     misc_feature    3994..4134
                     /label=AAV2 ITR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/name=AAV2 ITR"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=5b8101593b634e64aa9eef9434216f71"
                     /note="/vntifkey=21"
                     /note="color: #46c6ef; direction: RIGHT"
     misc_feature    5051..5911
                     /label=AmpR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=AmpR"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
                     /note="/vntifkey=21"
                     /note="color: #eda481; direction: RIGHT"
     misc_feature    6082..6670
                     /label=ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates low-copy plasmid number when Rop 
                     protein is presence (15-20) and medium-copy plasmid number 
                     when Rop is absent (100-300)."
                     /note="/full_name=pBR322 origin of replication"
                     /note="/name=ori"
                     /note="/official_designation=pBR322 ori"
                     /note="/uuid=b523c7b87d6149148546f5bcbd4831c3"
                     /note="/vntifkey=21"
                     /note="color: #da3bb5; direction: RIGHT"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtctgca gagggccctg cgtatgagtg
      181 caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc
      241 gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg
      301 gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca
      361 gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg
      421 acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc
      481 gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac
      541 catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga
      601 gtcgtgtcgt gcctgagagc gcagggtacc ggatcctcta gagtcgactc cggaacaact
      661 tcgtatatac ctttctatac gaagttgtgg cgcgccgcca ccatggagca gaagctgatc
      721 agcgaggagg acctgggcag tgtgagaacc aaccggtaca gcattgtctc ttcggaggaa
      781 gatggcatga agctggccac tatggcagtt gccaatggct ttgggaatgg caagagtaaa
      841 gtccataccc gacaacagtg caggagccgc tttgtgaaga aagatggtca ttgcaatgtt
      901 cagtttatca acgtgggtga gaagggacag aggtacctgg cagacatctt tactacctgt
      961 gtcgacatcc gctggaggtg gatgctggtt atcttctgtc ttgccttcgt gctctcctgg
     1021 ctgttctttg gctgtgtgtt ttggttgata gccctgctcc atggggatct agatacttct
     1081 aaagtgagca aagcatgcgt gtcggaggtt aacagcttca cggctgcctt cctcttctcc
     1141 atcgagaccc agacaaccat tggctatggt ttcaggtgtg tgacagacga atgcccaatt
     1201 gctgtcttca tggtggtatt ccagtcaatt gtaggctgca tcattgacgc cttcatcatt
     1261 ggtgcagtca tggcgaagat ggcaaagcca aagaagagaa atgagactct tgtcttcagt
     1321 cacaatgctg tgattgccat gagggatggc aaactctgct tgatgtggag agtgggtaac
     1381 cttcgaaaga gccaccttgt gggagctcat gtccgggcac agcttctcaa atctaggatc
     1441 acttcagaag gggagtttat ccctttggac cagatagaca tcaatgttgg ttttgatagt
     1501 ggaattgacc gcatatttct agtgtccccc atcactatcg ttcacgaaat agatgaagac
     1561 agccctttat atgacttgag taagcaggac attgacaatg cagactttga aattgttgtc
     1621 atactggaag gcatggtgga ggcgactgcc atgacaactc aatgccggag ttcgtatctg
     1681 gccaatgaaa ttctctgggg tcaccgctat gagccagtgc tctttgaaga gaaacactac
     1741 tataaagtag actattcaag attccataag acttatgaag tacctaacac ccccctttgt
     1801 agtgccagag acttagcaga gaagaaatac atcctttcaa atgcaaattc attttgctat
     1861 gaaaatgaag ttgccctaac aagcaaagag gaagaggagg atagtgagaa cggagtccca
     1921 gagagcacaa gcacagactc acctcctggc atagatctcc acaaccaggc aagcgtacct
     1981 ctagagccca ggcccttaag gcgagaatcg gagatagata acggaagcgg agccactaac
     2041 ttctccctgt tgaaacaagc aggggatgtc gaagagaatc ccgggccaat ggtgagcaag
     2101 ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     2161 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     2221 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     2281 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     2341 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     2401 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     2461 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     2521 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     2581 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     2641 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     2701 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     2761 ttcctgtacg gcatggacga gctgtacaag taagctagca caacttcgta tatacctttc
     2821 tatacgaagt tgtgaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     2881 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     2941 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3001 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3061 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3121 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3181 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3241 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct
     3301 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3361 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3421 gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg
     3481 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     3541 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     3601 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     3661 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     3721 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     3781 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     3841 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     3901 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     3961 ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     4021 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     4081 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     4141 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     4201 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4261 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4321 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4381 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     4441 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4501 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     4561 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4621 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     4681 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4741 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4801 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4861 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4921 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4981 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5041 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5101 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5161 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5221 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5281 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5341 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5401 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5461 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5521 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5581 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5641 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5701 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5761 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5821 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5881 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5941 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6001 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6061 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6121 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6181 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6241 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6301 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6361 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6421 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6481 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6541 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6601 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6661 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6721 tgctcacatg t
//
