LOCUS       pDis-ER/Golgi-APOX      6087 bp ds-DNA     linear       13-JUN-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: 2.64--lig pdct 6 -
            GFP-KDEL.str ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    222..389
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    390..593
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          638..657
                     /label="T7 forward sequencing primer"
                     /note="sequence: taatacgactcactataggg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    737..799
                     /label="Ig k-chain signal sequence"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer          756..845
                     /label="1.41-APEX2-Gib-F"
                     /note="sequence: ttccaggttccactggtgacggggcccagccggccagatctGACTACAAGGATGACGACGATAAGGGGAAATCATACCCAACAGTGTCCG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          772..824
                     /label="2.64-APEX2 GIB f"
                     /note="sequence: tgacggggcccagccggccagatctgactacaaggatgacgacgataagGTGG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    815..820
                     /label="BglII"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    821..845
                     /label="APEX plus mam codon optimized"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="locus_tag: APEX plus mam codon optimized(1) label: APEX plus mam codon optimized(1) ApEinfo_revcolor: #00ff00 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             821..1567
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAVHSAGTFDKGTKTGGPFGTIKHPAELAHIANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAVEVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHTIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDKYAADEDAFFADFAEAYQKLSELGFADA"
     misc_feature    846..1567
                     /label="APEX plus mam codon optimized"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="locus_tag: APEX plus mam codon optimized label: APEX plus mam codon optimized ApEinfo_revcolor: #00ff00 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    857..859
                     /label="K14D"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="locus_tag: K14D label: K14D ApEinfo_revcolor: cyan ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    938..940
                     /label="F41V"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="locus_tag: W41F label: W41F ApEinfo_revcolor: cyan ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             938..940
                     /label="Translation 938-940"
                     /translation="V"
     misc_feature    1022..1024
                     /label="S69I"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1151..1153
                     /label="E112K"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="locus_tag: E112K label: E112K ApEinfo_revcolor: cyan ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1217..1219
                     /label="A134P"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
                     /note="locus_tag: A134P label: A134P ApEinfo_revcolor: #ff0080 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1520..1522
                     /label="Y235F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1532..1534
                     /label="H239Y"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(1548..1637)
                     /label="2.64-APEX2 GIB R"
                     /note="sequence: cctcttctgagatgagtttttgttcgtcgacTGGCGCGCCACATTATTAcagctcgtccttGCCGCTGCCGGCGTCGGCAAATCCCAGTT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1568..1591
                     /label="FLAG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1592..1600
                     /label="3 aa spacer"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffff80 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1601..1612
                     /label="KDEL"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: #ff8000 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(1613..1618)
                     /label="double stop"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
                     /note="ApEinfo_revcolor: #800080 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1622..1629
                     /label="AscI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
                     /note="ApEinfo_revcolor: #ff0000 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1631..1636
                     /label="SalI"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
                     /note="ApEinfo_revcolor: #ff0080 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(1631..1636)
                     /label="New Feature"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #ff80c0 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer          complement(2653..2674)
                     /label="2.64-APEX2 GIB R"
                     /note="sequence: cctcttctgagatgagtttttgttcgtcgacTGGCGCGCCACATTATTAcagctcgtccttGCCGCTGCCGGCGTCGGCAAATCCCAGTT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgacg gggcccagcc ggccagatct GGGAAATCAT ACCCAACAGT
      841 GTCCGCAGAC TACCAGGATG CCGTGGAGAA AGCCAAGAAG AAGCTGAGAG GGTTTATCGC
      901 AGAAAAGAGG TGCGCACCTC TGATGCTGAG ACTGGCTGTC CACAGCGCAG GCACCTTTGA
      961 CAAGGGAACC AAAACAGGCG GACCCTTTGG AACAATCAAG CACCCTGCTG AACTGGCACA
     1021 TATTGCCAAC AATGGGCTGG ACATCGCCGT GCGGCTGCTG GAACCTCTGA AAGCAGAGTT
     1081 CCCAATTCTG TCCTACGCCG ATTTTTATCA GCTGGCAGGA GTGGTCGCTG TGGAGGTCAC
     1141 TGGGGGCCCC AAGGTGCCTT TCCACCCAGG ACGGGAGGAC AAACCAGAAC CACCTCCAGA
     1201 GGGGCGCCTG CCAGATccgA CAAAGGGCTC CGACCATCTG CGAGATGTGT TTGGGAAAGC
     1261 TATGGGCCTG ACTGACCAGG ATATCGTCGC ACTGTCTGGA GGGCACACCA TTGGCGCCGC
     1321 TCATAAGGAA AGGTCAGGCT TCGAGGGACC CTGGACAAGC AACCCCCTGA TTTTCGACAA
     1381 TTCTTACTTT ACTGAACTGC TGAGTGGAGA GAAGGAAGGG CTGCTGCAGC TGCCCAGCGA
     1441 CAAGGCCCTG CTGTCCGATC CCGTGTTCAG ACCTCTGGTC GATAAGTATG CAGCCGACGA
     1501 GGATGCTTTT TTCGCAGATT TCGCAGAAGC ATATCAGAAG CTGTCAGAAC TGGGATTTGC
     1561 CGACGCCGAC TACAAGGATG ACGACGATAA GGGCAGCGGC aaggacgagc tgTAATAATG
     1621 TGGCGCGCCA gtcgacgaac aaaaactcat ctcagaagag gatctgaatg ctgtgggcca
     1681 ggacacgcag gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg tggtgatctc
     1741 agccatcctg gccctggtgg tgctcaccat catctccctt atcatcctca tcatgcttgg
     1801 cagaagaagc cacgttaggc ggccgctcga gatcagcctc gactgtgcct tctagttgcc
     1861 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1921 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1981 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     2041 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagt ggcggtaata
     2101 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2161 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2221 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2281 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2341 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2401 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2461 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2521 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2581 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     2641 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2701 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2761 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2821 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2881 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2941 taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca
     3001 cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg
     3061 ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca
     3121 aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct
     3181 tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc
     3241 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     3301 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc
     3361 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     3421 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     3481 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     3541 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     3601 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     3661 tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tgatcatcct
     3721 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     3781 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     3841 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     3901 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     3961 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac
     4021 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     4081 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     4141 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     4201 ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
     4261 tggaatagct cagaggccga ggaggcggcc tcggcctctg cataaataaa aaaaattagt
     4321 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     4381 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     4441 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     4501 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacagc
     4561 tggttctttc cgcctcagga ctcttccttt ttcaataaat caatctaaag tatatatgag
     4621 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4681 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4741 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4801 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4861 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4921 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4981 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5041 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5101 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5161 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5221 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5281 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5341 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5401 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5461 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5521 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5581 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     5641 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5701 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5761 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5821 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5881 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5941 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6001 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6061 aacaaaatat taacgcttac aatttac
//