LOCUS       RdRp~mScarlet3~I       12861 bp    DNA     circular SYN 19-SEP-2024
DEFINITION  mSc3-IRES.EGFP-IRES.E3.
ACCESSION   mSc3-IRES.EGFP-IRES.E3
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..12861
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     promoter        610..627
                     /label=T7 promoter
                     /label=T7 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=94.7"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     CDS             672..8150
                     /codon_start=1
                     /label=Venezuelan equine encephalitis virus nsP1234
                     /label=POLN_EEVVT
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #333399"
                     /translation="MEKVHVDIEEDSPFLRALQRSFPQFEVEAKQVTDNDHANARAFSH
                     LASKLIETEVDPSDTILDIGSAPARRMYSKHKYHCICPMRCAEDPDRLYKYATKLKKNC
                     KEITDKELDKKMKELAAVMSDPDLETETMCLHDDESCRYEGQVAVYQDVYAVDGPTSLY
                     HQANKGVRVAYWIGFDTTPFMFKNLAGAYPSYSTNWADETVLTARNIGLCSSDVMERSR
                     RGMSILRKKYLKPSNNVLFSVGSTIYHEKRDLLRSWHLPSVFHLRGKQNYTCRCETIVS
                     CDGYVVKRIAISPGLYGKPSGYAATMHREGFLCCKVTDTLNGERVSFPVCTYVPATLCD
                     QMTGILATDVSADDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQAFARWAKEYK
                     EDQEDERPLGLRDRQLVMGCCWAFRRHKITSIYKRPDTQTIIKVNSDFHSFVLPRIGSN
                     TLEIGLRTRIRKMLEEHKEPSPLITAEDVQEAKCAADEAKEVREAEELRAALPPLAADV
                     EEPTLEADVDLMLQEAGAGSVETPRGLIKVTSYDGEDKIGSYAVLSPQAVLKSEKLSCI
                     HPLAEQVIVITHSGRKGRYAVEPYHGKVVVPEGHAIPVQDFQALSESATIVYNEREFVN
                     RYLHHIATHGGALNTDEEYYKTVKPSEHDGEYLYDIDRKQCVKKELVTGLGLTGELVDP
                     PFHEFAYESLRTRPAAPYQVPTIGVYGVPGSGKSGIIKSAVTKKDLVVSAKKENCAEII
                     RDVKKMKGLDVNARTVDSVLLNGCKHPVETLYIDEAFACHAGTLRALIAIIRPKKAVLC
                     GDPKQCGFFNMMCLKVHFNHEICTQVFHKSISRRCTKSVTSVVSTLFYDKKMRTTNPKE
                     TKIVIDTTGSTKPKQDDLILTCFRGWVKQLQIDYKGNEIMTAAASQGLTRKGVYAVRYK
                     VNENPLYAPTSEHVNVLLTRTEDRIVWKTLAGDPWIKTLTAKYPGNFTATIEEWQAEHD
                     AIMRHILERPDPTDVFQNKANVCWAKALVPVLKTAGIDMTTEQWNTVDYFETDKAHSAE
                     IVLNQLCVRFFGLDLDSGLFSAPTVPLSIRNNHWDNSPSPNMYGLNKEVVRQLSRRYPQ
                     LPRAVATGRVYDMNTGTLRNYDPRINLVPVNRRLPHALVLHHNEHPQSDFSSFVSKLKG
                     RTVLVVGEKLSVPGKMVDWLSDRPEATFRARLDLGIPGDVPKYDIIFVNVRTPYKYHHY
                     QQCEDHAIKLSMLTKKACLHLNPGGTCVSIGYGYADRASESIIGAIARQFKFSRVCKPK
                     SSLEETEVLFVFIGYDRKARTHNSYKLSSTLTNIYTGSRLHEAGCAPSYHVVRGDIATA
                     TEGVIINAANSKGQPGGGVCGALYKKFPESFDLQPIEVGKARLVKGAAKHIIHAVGPNF
                     NKVSEVEGDKQLAEAYESIAKIVNDNNYKSVAIPLLSTGIFSGNKDRLTQSLNHLLTAL
                     DTTDADVAIYCRDKKWEMTLKEAVARREAVEEICISDDSSVTEPDAELVRVHPKSSLAG
                     RKGYSTSDGKTFSYLEGTKFHQAAKDIAEINAMWPVATEANEQVCMYILGESMSSIRSK
                     CPVEESEASTPPSTLPCLCIHAMTPERVQRLKASRPEQITVCSSFPLPKYRITGVQKIQ
                     CSQPILFSPKVPAYIHPRKYLVETPPVDETPEPSAENQSTEGTPEQPPLITEDETRTRT
                     PEPIIIEEEEEDSISLLSDGPTHQVLQVEADIHGPPSVSSSSWSIPHASDFDVDSLSIL
                     DTLEGASVTSGATSAETNSYFAKSMEFLARPVPAPRTVFRNPPHPAPRTRTPSLAPSRA
                     CSRTSLVSTPPGVNRVITREELEALTPSRTPSRSVSRTSLVSNPPGVNRVITREEFEAF
                     VAQQQ*RFDAGAYIFSSDTGQGHLQQKSVRQTVLSEVVLERTELEISYAPRLDQEKEEL
                     LRKKLQLNPTPANRSRYQSRKVENMKAITARRILQGLGHYLKAEGKVECYRTLHPVPLY
                     SSSVNRAFSSPKVAVEACNAMLKENFPTVASYCIIPEYDAYLDMVDGASCCLDTASFCP
                     AKLRSFPKKHSYLEPTIRSAVPSAIQNTLQNVLAAATKRNCNVTQMRELPVLDSAAFNV
                     ECFKKYACNNEYWETFKENPIRLTEENVVNYITKLKGPKAAALFAKTHNLNMLQDIPMD
                     RFVMDLKRDVKVTPGTKHTEERPKVQVIQAADPLATAYLCGIHRELVRRLNAVLLPNIH
                     TLFDMSAEDFDAIIAEHFQPGDCVLETDIASFDKSEDDAMALTALMILEDLGVDAELLT
                     LIEAAFGEISSIHLPTKTKFKFGAMMKSGMFLTLFVNTVINIVIASRVLRERLTGSPCA
                     AFIGDDNIVKGVKSDKLMADRCATWLNMEVKIIDAVVGEKAPYFCGGFILCDSVTGTAC
                     RVADPLKRLFKLGKPLAADDEHDDDRRRALHEESTRWNRVGILSELCKAVESRYETVGT
                     SIIVMAMTTLASSVKSFSYLRGAPITLYG"
     CDS             8214..8900
                     /codon_start=1
                     /label=mScarlet3
                     /label=mScarlet-2I (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=97.7"
                     /note="/other=CDS"
                     /note="color: #ff0000"
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     misc_feature    8917..9504
                     /label=IRES2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.2"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     CDS             9505..10221
                     /codon_start=1
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #00ff00"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    10282..10843
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.1"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     CDS             10846..11415
                     /codon_start=1
                     /label=E3
                     /label=E3_VACCW
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #00ffff"
                     /translation="MSKIYIDERSNAEIVCEAIKTIGIEGATAAQLTRQLNMEKREVNK
                     ALYDLQRSAMVYSSDDIPPRWFMTTEADKPDADAMADVIIDDVSREKSMREDHKSFDDV
                     IPAKKIIDWKGANPVTVINEYCQITRRDWSFRIESVGPSNSPTFYACVDIDGRVFDKAD
                     GKSKRDAKNNAAKLAVDKLLGYVIIRF"
     promoter        11726..11830
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             11831..12691
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagctct aatacgactc actatagatg ggcggcgcat gagagaagcc cagaccaatt
      661 acctacccaa aatggagaaa gttcacgttg acatcgagga agacagccca ttcctcagag
      721 ctttgcagcg gagcttcccg cagtttgagg tagaagccaa gcaggtcact gataatgacc
      781 atgctaatgc cagagcgttt tcgcatctgg cttcaaaact gatcgaaacg gaggtggacc
      841 catccgacac gatccttgac attggaagtg cgcccgcccg cagaatgtat tctaagcaca
      901 agtatcattg tatctgtccg atgagatgtg cggaagatcc ggacagattg tataagtatg
      961 caactaagct gaagaaaaac tgtaaggaaa taactgataa ggaattggac aagaaaatga
     1021 aggagctggc cgccgtcatg agcgaccctg acctggaaac tgagactatg tgcctccacg
     1081 acgacgagtc gtgtcgctac gaagggcaag tcgctgttta ccaggatgta tacgcggttg
     1141 acggaccgac aagtctctat caccaagcca ataagggagt tagagtcgcc tactggatag
     1201 gctttgacac cacccctttt atgtttaaga acttggctgg agcatatcca tcatactcta
     1261 ccaactgggc cgacgaaacc gtgttaacgg ctcgtaacat aggcctatgc agctctgacg
     1321 ttatggagcg gtcacgtaga gggatgtcca ttcttagaaa gaagtatttg aaaccatcca
     1381 acaatgttct attctctgtt ggctcgacca tctaccacga gaagagggac ttactgagga
     1441 gctggcacct gccgtctgta tttcacttac gtggcaagca aaattacaca tgtcggtgtg
     1501 agactatagt tagttgcgac gggtacgtcg ttaaaagaat agctatcagt ccaggcctgt
     1561 atgggaagcc ttcaggctat gctgctacga tgcaccgcga gggattcttg tgctgcaaag
     1621 tgacagacac attgaacggg gagagggtct cttttcccgt gtgcacgtat gtgccagcta
     1681 cattgtgtga ccaaatgact ggcatactgg caacagatgt cagtgcggac gacgcgcaaa
     1741 aactgctggt tgggctcaac cagcgtatag tcgtcaacgg tcgcacccag agaaacacca
     1801 ataccatgaa aaattacctt ttgcccgtag tggcccaggc atttgctagg tgggcaaagg
     1861 aatataagga agatcaagaa gatgaaaggc cactaggact acgagataga cagttagtca
     1921 tggggtgttg ttgggctttt agaaggcaca agataacatc tatttataag cgcccggata
     1981 cccaaaccat catcaaagtg aacagcgatt tccactcatt cgtgctgccc aggataggca
     2041 gtaacacatt ggagatcggg ctgagaacaa gaatcaggaa aatgttagag gagcacaagg
     2101 agccgtcacc tctcattacc gccgaggacg tacaagaagc taagtgcgca gccgatgagg
     2161 ctaaggaggt gcgtgaagcc gaggagttgc gcgcagctct accacctttg gcagctgatg
     2221 ttgaggagcc cactctggag gcagacgtcg acttgatgtt acaagaggct ggggccggct
     2281 cagtggagac acctcgtggc ttgataaagg ttaccagcta cgatggcgag gacaagatcg
     2341 gctcttacgc tgtgctttct ccgcaggctg tactcaagag tgaaaaatta tcttgcatcc
     2401 accctctcgc tgaacaagtc atagtgataa cacactctgg ccgaaaaggg cgttatgccg
     2461 tggaaccata ccatggtaaa gtagtggtgc cagagggaca tgcaataccc gtccaggact
     2521 ttcaagctct gagtgaaagt gccaccattg tgtacaacga acgtgagttc gtaaacaggt
     2581 acctgcacca tattgccaca catggaggag cgctgaacac tgatgaagaa tattacaaaa
     2641 ctgtcaagcc cagcgagcac gacggcgaat acctgtacga catcgacagg aaacagtgcg
     2701 tcaagaaaga actagtcact gggctagggc tcacaggcga gctggtggat cctcccttcc
     2761 atgaattcgc ctacgagagt ctgagaacac gaccagccgc tccttaccaa gtaccaacca
     2821 taggggtgta tggcgtgcca ggatcaggca agtctggcat cattaaaagc gcagtcacca
     2881 aaaaagatct agtggtgagc gccaagaaag aaaactgtgc agaaattata agggacgtca
     2941 agaaaatgaa agggctggac gtcaatgcca gaactgtgga ctcagtgctc ttgaatggat
     3001 gcaaacaccc cgtagagacc ctgtatattg acgaagcttt tgcttgtcat gcaggtactc
     3061 tcagagcgct catagccatt ataagaccta aaaaggcagt gctctgcggg gatcccaaac
     3121 agtgcggttt ttttaacatg atgtgcctga aagtgcattt taaccacgag atttgcacac
     3181 aagtcttcca caaaagcatc tctcgccgtt gcactaaatc tgtgacttcg gtcgtctcaa
     3241 ccttgtttta cgacaaaaaa atgagaacga cgaatccgaa agagactaag attgtgattg
     3301 acactaccgg cagtaccaaa cctaagcagg acgatctcat tctcacttgt ttcagagggt
     3361 gggtgaagca gttgcaaata gattacaaag gcaacgaaat aatgacggca gctgcctctc
     3421 aagggctgac ccgtaaaggt gtgtatgccg ttcggtacaa ggtgaatgaa aatcctctgt
     3481 acgcacccac ctcagaacat gtgaacgtcc tactgacccg cacggaggac cgcatcgtgt
     3541 ggaaaacact agccggcgac ccatggataa aaacactgac tgccaagtac cctgggaatt
     3601 tcactgccac gatagaggag tggcaagcag agcatgatgc catcatgagg cacatcttgg
     3661 agagaccgga ccctaccgac gtcttccaga ataaggcaaa cgtgtgttgg gccaaggctt
     3721 tagtgccggt gctgaagacc gctggcatag acatgaccac tgaacaatgg aacactgtgg
     3781 attattttga aacggacaaa gctcactcag cagagatagt attgaaccaa ctatgcgtga
     3841 ggttctttgg actcgatctg gactccggtc tattttctgc acccactgtt ccgttatcca
     3901 ttaggaataa tcactgggat aactccccgt cgcctaacat gtacgggctg aataaagaag
     3961 tggtccgtca gctctctcgc aggtacccac aactgcctcg ggcagttgcc actggaagag
     4021 tctatgacat gaacactggt acactgcgca attatgatcc gcgcataaac ctagtacctg
     4081 taaacagaag actgcctcat gctttagtcc tccaccataa tgaacaccca cagagtgact
     4141 tttcttcatt cgtcagcaaa ttgaagggca gaactgtcct ggtggtcggg gaaaagttgt
     4201 ccgtcccagg caaaatggtt gactggttgt cagaccggcc tgaggctacc ttcagagctc
     4261 ggctggattt aggcatccca ggtgatgtgc ccaaatatga cataatattt gttaatgtga
     4321 ggaccccata taaataccat cactatcagc agtgtgaaga ccatgccatt aagcttagca
     4381 tgttgaccaa gaaagcttgt ctgcatctga atcccggcgg aacctgtgtc agcataggtt
     4441 atggttacgc tgacagggcc agcgaaagca tcattggtgc tatagcgcgg cagttcaagt
     4501 tttcccgggt atgcaaaccg aaatcctcac ttgaagagac ggaagttctg tttgtattca
     4561 ttgggtacga tcgcaaggcc cgtacgcaca attcttacaa gctttcatca accttgacca
     4621 acatttatac aggttccaga ctccacgaag ccggatgtgc accctcatat catgtggtgc
     4681 gaggggatat tgccacggcc accgaaggag tgattataaa tgctgctaac agcaaaggac
     4741 aacctggcgg aggggtgtgc ggagcgctgt ataagaaatt cccggaaagc ttcgatttac
     4801 agccgatcga agtaggaaaa gcgcgactgg tcaaaggtgc agctaaacat atcattcatg
     4861 ccgtaggacc aaacttcaac aaagtttcgg aggttgaagg tgacaaacag ttggcagagg
     4921 cttatgagtc catcgctaag attgtcaacg ataacaatta caagtcagta gcgattccac
     4981 tgttgtccac cggcatcttt tccgggaaca aagatcgact aacccaatca ttgaaccatt
     5041 tgctgacagc tttagacacc actgatgcag atgtagccat atactgcagg gacaagaaat
     5101 gggaaatgac tctcaaggaa gcagtggcta ggagagaagc agtggaggag atatgcatat
     5161 ccgacgactc ttcagtgaca gaacctgatg cagagctggt gagggtgcat ccgaagagtt
     5221 ctttggctgg aaggaagggc tacagcacaa gcgatggcaa aactttctca tatttggaag
     5281 ggaccaagtt tcaccaggcg gccaaggata tagcagaaat taatgccatg tggcccgttg
     5341 caacggaggc caatgagcag gtatgcatgt atatcctcgg agaaagcatg agcagtatta
     5401 ggtcgaaatg ccccgtcgaa gagtcggaag cctccacacc acctagcacg ctgccttgct
     5461 tgtgcatcca tgccatgact ccagaaagag tacagcgcct aaaagcctca cgtccagaac
     5521 aaattactgt gtgctcatcc tttccattgc cgaagtatag aatcactggt gtgcagaaga
     5581 tccaatgctc ccagcctata ttgttctcac cgaaagtgcc tgcgtatatt catccaagga
     5641 agtatctcgt ggaaacacca ccggtagacg agactccgga gccatcggca gagaaccaat
     5701 ccacagaggg gacacctgaa caaccaccac ttataaccga ggatgagacc aggactagaa
     5761 cgcctgagcc gatcatcatc gaagaggaag aagaggatag cataagtttg ctgtcagatg
     5821 gcccgaccca ccaggtgctg caagtcgagg cagacattca cgggccgccc tctgtatcta
     5881 gctcatcctg gtccattcct catgcatccg actttgatgt ggacagttta tccatacttg
     5941 acaccctgga gggagctagc gtgaccagcg gggcaacgtc agccgagact aactcttact
     6001 tcgcaaagag tatggagttt ctggcgcgac cggtgcctgc gcctcgaaca gtattcagga
     6061 accctccaca tcccgctccg cgcacaagaa caccgtcact tgcacccagc agggcctgct
     6121 cgagaaccag cctagtttcc accccgccag gcgtgaatag ggtgatcact agagaggagc
     6181 tcgaggcgct taccccgtca cgcactccta gcaggtcggt ctcgagaacc agcctggtct
     6241 ccaacccgcc aggcgtaaat agggtgatta caagagagga gtttgaggcg ttcgtagcac
     6301 aacaacaatg acggtttgat gcgggtgcat acatcttttc ctccgacacc ggtcaagggc
     6361 atttacaaca aaaatcagta aggcaaacgg tgctatccga agtggtgttg gagaggaccg
     6421 aattggagat ttcgtatgcc ccgcgcctcg accaagaaaa agaagaatta ctacgcaaga
     6481 aattacagtt aaatcccaca cctgctaaca gaagcagata ccagtccagg aaggtggaga
     6541 acatgaaagc cataacagct agacgtattc tgcaaggcct agggcattat ttgaaggcag
     6601 aaggaaaagt ggagtgctac cgaaccctgc atcctgttcc tttgtattca tctagtgtga
     6661 accgtgcctt ttcaagcccc aaggtcgcag tggaagcctg taacgccatg ttgaaagaga
     6721 actttccgac tgtggcttct tactgtatta ttccagagta cgatgcctat ttggacatgg
     6781 ttgacggagc ttcatgctgc ttagacactg ccagtttttg ccctgcaaag ctgcgcagct
     6841 ttccaaagaa acactcctat ttggaaccca caatacgatc ggcagtgcct tcagcgatcc
     6901 agaacacgct ccagaacgtc ctggcagctg ccacaaaaag aaattgcaat gtcacgcaaa
     6961 tgagagaatt gcccgtattg gattcggcgg cctttaatgt ggaatgcttc aagaaatatg
     7021 cgtgtaataa tgaatattgg gaaacgttta aagaaaaccc catcaggctt actgaagaaa
     7081 acgtggtaaa ttacattacc aaattaaaag gaccaaaagc tgctgctctt tttgcgaaga
     7141 cacataattt gaatatgttg caggacatac caatggacag gtttgtaatg gacttaaaga
     7201 gagacgtgaa agtgactcca ggaacaaaac atactgaaga acggcccaag gtacaggtga
     7261 tccaggctgc cgatccgcta gcaacagcgt atctgtgcgg aatccaccga gagctggtta
     7321 ggagattaaa tgcggtcctg cttccgaaca ttcatacact gtttgatatg tcggctgaag
     7381 actttgacgc tattatagcc gagcacttcc agcctgggga ttgtgttctg gaaactgaca
     7441 tcgcgtcgtt tgataaaagt gaggacgacg ccatggctct gaccgcgtta atgattctgg
     7501 aagacttagg tgtggacgca gagctgttga cgctgattga ggcggctttc ggcgaaattt
     7561 catcaataca tttgcccact aaaactaaat ttaaattcgg agccatgatg aaatctggaa
     7621 tgttcctcac actgtttgtg aacacagtca ttaacattgt aatcgcaagc agagtgttga
     7681 gagaacggct aaccggatca ccatgtgcag cattcattgg agatgacaat atcgtgaaag
     7741 gagtcaaatc ggacaaatta atggcagaca ggtgcgccac ctggttgaat atggaagtca
     7801 agattataga tgctgtggtg ggcgagaaag cgccttattt ctgtggaggg tttattttgt
     7861 gtgactccgt gaccggcaca gcgtgccgtg tggcagaccc cctaaaaagg ctgtttaagc
     7921 ttggcaaacc tctggcagca gacgatgaac atgatgatga caggagaagg gcattgcatg
     7981 aagagtcaac acgctggaac cgagtgggta ttctttcaga gctgtgcaag gcagtagaat
     8041 caaggtatga aaccgtagga acttccatca tagttatggc catgactact ctagctagca
     8101 gtgttaaatc attcagctac ctgagagggg cccctataac tctctacggc taacctgaat
     8161 ggactacgac atagtctagt ccgccaagtc tagcaattgg aattccggcc accatggata
     8221 gcaccgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag ggctccatga
     8281 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     8341 ccgccaagct gagggtgacc aagggtggcc ccctgccctt ctcctgggac atcctgtccc
     8401 ctcagttcat gtacggctcc agggccttca cgaagcaccc cgccgacatc cccgactact
     8461 ggaagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     8521 gcgccgtgtc cgtggcccag gacacctccc tggaggacgg caccctgatc tacaaggtga
     8581 agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag acaatgggct
     8641 gggaagcatc caccgagcgg ttgtaccccg aggacgtcgt gctgaagggc gacattaaga
     8701 tggccctgcg cctgaaggac ggaggacgct acctggcgga cttcaagacc acctacaggg
     8761 ccaagaagcc cgtgcagatg cccggcgcct tcaacatcga ccgcaagttg gacatcacat
     8821 cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgtggcc cgccactcca
     8881 ccggcggctc cggtggctcc taacatatgt gatccgcccc tctccctccc ccccccccta
     8941 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     9001 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     9061 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     9121 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     9181 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     9241 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     9301 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     9361 taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt
     9421 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     9481 cacgatgata atatggccac aaccatggtg agcaagggcg aggagctgtt caccggggtg
     9541 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     9601 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     9661 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     9721 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     9781 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     9841 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     9901 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     9961 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
    10021 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
    10081 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
    10141 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
    10201 ggcatggacg agctgtacaa gtaaagcggc cgcaactaac ttaagctagc aacggtttcc
    10261 ctctagcggg atcaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat
    10321 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
    10381 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
    10441 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
    10501 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
    10561 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
    10621 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
    10681 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
    10741 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
    10801 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatgag caagatctac
    10861 atcgacgagc ggagcaacgc cgagattgtg tgcgaggcca tcaagaccat cggaatcgaa
    10921 ggcgccacag ccgctcagct gaccagacag ctgaacatgg aaaagcggga agtgaacaag
    10981 gccctgtacg acctgcagag aagcgccatg gtgtacagca gcgacgacat ccctcctcgg
    11041 tggtttatga ccacagaggc cgacaagcct gacgccgatg ctatggccga cgtgatcatc
    11101 gacgacgtgt cccgcgagaa gtccatgaga gaggaccaca agagcttcga cgatgtgatc
    11161 cccgccaaga agatcatcga ttggaagggc gccaatcctg tgaccgtgat caacgagtac
    11221 tgccagatca ccagaagaga ctggtccttc cggatcgaga gcgtgggccc tagcaatagc
    11281 cctaccttct acgcctgcgt ggacatcgac ggcagagtgt tcgataaggc cgacggcaag
    11341 agcaagcggg acgccaaaaa caatgccgcc aagctggccg tggataagct gctgggctat
    11401 gtgatcatcc ggttctaagg gcaagctgct tacatagaac tcgcggcgat tggcatgccg
    11461 ccttaaaatt tttattttat tttttctttt cttttccgaa tcggattttg tttttaatat
    11521 ttcaaaaaaa aaaaaaaaaa aaaacgcgaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
    11581 ttaataaaaa aaaaaaaaaa aaaaaaaaaa aaaattaata aaaaaaaaaa aaaaaaaaaa
    11641 aaaaaaatta attaatctag aacgcgtcga ggggaattaa ttcttgaaga cgaaagggcc
    11701 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    11761 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    11821 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    11881 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    11941 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
    12001 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    12061 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    12121 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    12181 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    12241 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    12301 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    12361 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    12421 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    12481 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    12541 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    12601 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    12661 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    12721 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    12781 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    12841 agaaaagatc aaaggatctt c
//
