LOCUS       pVBL0001_deltaLD-IRE1-m 9130 bp ds-DNA     circular     07-JUL-2025
DEFINITION  .
KEYWORDS    "marker:AmpR, HygR"
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        615..818
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    820..838
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    841..859
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        996..1014
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    1044..1068
                     /label="attB1"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     misc_feature    1074..1151
                     /label="Lumenal"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1152..1214
                     /label="TM"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1231..1355
                     /label="Q Box"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1356..1532
                     /label="S Box"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    1533..2318
                     /label="Kinase"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2334..2687
                     /label="RNase"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2688..2753
                     /label="Proline Rich Region"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             2754..2783
                     /label="GS 10x v1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             2784..3491
                     /label="mCherry"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             3498..4991
                     /label="Cry2clust"
                     /ApEinfo_revcolor="#666699"
                     /ApEinfo_fwdcolor="#666699"
     CDS             4992..5018
                     /label="Cry2clust"
                     /ApEinfo_revcolor="#666699"
                     /ApEinfo_fwdcolor="#666699"
     misc_feature    5007..5018
                     /label="Mod"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    complement(5023..5047)
                     /label="attB2"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     polyA_signal    5088..5312
                     /label="BGH pA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    5596..5643
                     /label="FRT"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     CDS             5651..6673
                     /label="HygR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    6803..6924
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(6973..6989)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    6997..7013
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(7021..7027)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(7028..7045)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(7046..7051)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    7066..7087
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(7309..7989)
                     /label="pUC Ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(8134..8925)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(8926..8994)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(8995..9099)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgcttatc gaaattaata cgactcacta tagggagacc
     1021 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttc accATGCCGG
     1081 CCCGGCGGCT GCTGCTGCTG CTGACGCTGC TGCTGCCCGG CCTCGGGATT TTTGGAAGTA
     1141 TGCTTAAGGA CATGGCTACC ATCATCCTGA GCACCTTCCT GCTGATTGGC TGGGTGGCCT
     1201 TCATCATCAC CTATCCCCTG AGCATGCATC AGCAGCAGCA GCTCCAGCAC CAGCAGTTCC
     1261 AGAAGGAACT GGAGAAGATC CAGCTCCTGC AGCAGCAGCA GCAGCAGCTG CCCTTCCACC
     1321 CACCTGGAGA CACGGCTCAG GACGGCGAGC TCCTGGACAC GTCTGGCCCG TACTCAGAGA
     1381 GCTCGGGCAC CAGCAGCCCC AGCACGTCCC CCAGGGCCTC CAACCACTCG CTCTGCTCCG
     1441 GCAGCTCTGC CTCCAAGGCT GGCAGCAGCC CCTCCCTGGA ACAAGACGAT GGAGATGAGG
     1501 AAACCAGCGT GGTGATAGTT GGGAAAATTT CCTTCTGTCC CAAGGATGTC CTGGGCCATG
     1561 GAGCTGAGGG CACAATTGTG TACCGGGGCA TGTTTGACAA CCGCGACGTG GCCGTGAAGA
     1621 GGATCCTCCC CGAGTGTTTT AGCTTCGCAG ACCGTGAGGT CCAGCTGTTG CGAGAATCGG
     1681 ATGAGCACCC GAACGTGATC CGCTACTTCT GCACGGAGAA GGACCGGCAA TTCCAGTACA
     1741 TTGCCATCGA GCTGTGTGCA GCCACCCTGC AAGAGTATGT GGAGCAGAAG GACTTTGCGC
     1801 ATCTCGGCCT GGAGCCCATC ACCTTGCTGC AGCAGACCAC CTCGGGCCTG GCCCACCTCC
     1861 ACTCCCTCAA CATCGTTCAC AGAGACCTAA AGCCACACAA CATCCTCATA TCCATGCCCA
     1921 ATGCACACGG CAAGATCAAG GCCATGATCT CCGACTTTGG CCTCTGCAAG AAGCTGGCAG
     1981 TGGGCAGACA CAGTTTCAGC CGCCGATCTG GGGTGCCTGG CACAGAAGGC TGGATCGCTC
     2041 CAGAGATGCT GAGCGAAGAC TGTAAGGAGA ACCCTACCTA CACGGTGGAC ATCTTTTCTG
     2101 CAGGCTGCGT CTTTTACTAC GTAATCTCTG AGGGCAGCCA CCCTTTTGGC AAGTCCCTGC
     2161 AGCGGCAGGC CAACATCCTC CTGGGTGCCT GCAGCCTTGA CTGCTTGCAC CCAGAGAAGC
     2221 ACGAAGACGT CATTGCACGT GAATTGATAG AGAAGATGAT TGCGATGGAT CCTCAGAAAC
     2281 GCCCCTCAGC GAAGCACGTG CTCAAACACC CGTTCTTCTG GAGCCTAGAG AAGCAGCTCC
     2341 AGTTCTTCCA GGACGTGAGC GACAGAATAG AAAAGGAATC CCTGGATGGC CCGATCGTGA
     2401 AGCAGTTAGA GAGAGGCGGG AGAGCCGTGG TGAAGATGGA CTGGCGGGAG AACATCACTG
     2461 TCCCCCTCCA GACAGACCTG CGTAAATTCA GGACCTATAA AGGTGGTTCT GTCAGAGATC
     2521 TCCTCCGAGC CATGAGAAAT AAGAAGCACC ACTACCGGGA GCTGCCTGCA GAGGTGCGGG
     2581 AGACGCTGGG GTCCCTCCCC GACGACTTCG TGTGCTACTT CACGTCTCGC TTCCCCCACC
     2641 TCCTCGCACA CACCTACCGG GCCATGGAGC TGTGCAGCCA CGAGAGACTC TTCCAGCCCT
     2701 ACTACTTCCA CGAGCCCCCA GAGCCCCAGC CCCCAGTGAC TCCAGACGCC CTCggAggaT
     2761 CTGGAGGCGG CggGTCCGGC ggaatggtgt ctaaaggcga ggaggataac atggctataa
     2821 tcaaggaatt tatgaggttc aaggtgcata tggagggatc tgtgaacggt cacgagttcg
     2881 aaatcgaggg cgaaggcgag gggcgaccct atgaagggac acagacggcc aaactgaagg
     2941 tgaccaaggg tggccccctg cctttcgcct gggacatcct gtcaccccag tttatgtacg
     3001 gctctaaggc ttacgttaag caccctgccg atattccgga ctacttgaaa ctgagcttcc
     3061 ctgaagggtt caagtgggaa cgggtaatga attttgagga tggcggtgta gtcacagtta
     3121 cccaggacag ctctcttcag gacggagaat ttatctataa ggttaaactt cggggcacta
     3181 acttcccatc agacggcccc gtgatgcaga agaaaactat gggctgggaa gccagctcag
     3241 aacgcatgta tcccgaggac ggagcactga agggagaaat caagcagcga ctcaagctga
     3301 aggatggggg acattatgat gctgaggtca aaaccaccta taaggccaag aaacccgttc
     3361 agcttcctgg ggcatacaac gtgaatatca aactggatat tacatcccat aacgaagact
     3421 ataccatcgt ggaacagtac gagcgggccg agggcagaca tagcacaggg ggcatggatg
     3481 aattgtacaa ggcggccatg aagatggaca aaaagactat agtttggttt agaagagacc
     3541 taaggattga ggataatcct gcattagcag cagctgctca cgaaggatct gtttttcctg
     3601 tcttcatttg gtgtcctgaa gaagaaggac agttttatcc tggaagagct tcaagatggt
     3661 ggatgaaaca atcacttgct cacttatctc aatccttgaa ggctcttgga tctgacctca
     3721 ctttaatcaa aacccacaac acgatttcag cgatcttgga ttgtatccgc gttaccggtg
     3781 ctacaaaagt cgtctttaac cacctctatg atcctgtttc gttagttcgg gaccataccg
     3841 taaaggagaa gctggtggaa cgtgggatct ctgtgcaaag ctacaatgga gatctattgt
     3901 atgaaccgtg ggagatatac tgcgaaaagg gcaaaccttt tacgagtttc aattcttact
     3961 ggaagaaatg cttagatatg tcgattgaat ccgttatgct tcctcctcct tggcggttga
     4021 tgccaataac tgcagcggct gaagcgattt gggcgtgttc gattgaagaa ctagggctgg
     4081 agaatgaggc cgagaaaccg agcaatgcgt tgttaactag agcttggtct ccaggatgga
     4141 gcaatgctga taagttacta aatgagttca tcgagaagca gttgatagat tatgcaaaga
     4201 acagcaagaa agttgttggg aattctactt cactactttc tccgtatctc catttcgggg
     4261 aaataagcgt cagacacgtt ttccagtgtg cccggatgaa acaaattata tgggcaagag
     4321 ataagaacag tgaaggagaa gaaagtgcag atctttttct taggggaatc ggtttaagag
     4381 agtattctcg gtatatatgt ttcaacttcc cgtttactca cgagcaatcg ttgttgagtc
     4441 atcttcggtt tttcccttgg gatgctgatg ttgataagtt caaggcctgg agacaaggca
     4501 ggaccggtta tccgttggtg gatgccggaa tgagagagct ttgggctacc ggatggatgc
     4561 ataacagaat aagagtgatt gtttcaagct ttgctgtgaa gtttcttctc cttccatgga
     4621 aatggggaat gaagtatttc tgggatacac ttttggatgc tgatttggaa tgtgacatcc
     4681 ttggctggca gtatatctct gggagtatcc ccgatggcca cgagcttgat cgcttggaca
     4741 atcccgcgtt acaaggcgcc aaatatgacc cagaaggtga gtacataagg caatggcttc
     4801 ccgagcttgc gagattgcca actgaatgga tccatcatcc atgggacgct cctttaaccg
     4861 tactcaaagc ttctggtgtg gaactcggaa caaactatgc gaaacccatt gtagacatcg
     4921 acacagctcg tgagctacta gctaaagcta tttcaagaac ccgtgaagca cagatcatga
     4981 tcggagcagc agcccgggat ccaccggatc tggataactg acacccagct ttcttgtaca
     5041 aagtggttga tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     5101 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     5161 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     5221 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     5281 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     5341 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5401 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5461 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5521 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5581 tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     5641 ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     5701 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     5761 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     5821 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     5881 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     5941 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     6001 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     6061 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     6121 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     6181 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     6241 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     6301 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     6361 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     6421 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     6481 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     6541 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     6601 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     6661 ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt
     6721 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     6781 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     6841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6901 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     6961 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7801 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9121 acctgacgtc
//