LOCUS       pCMV-Gaussia_Luciferase 6027 bp ds-DNA     circular     26-JAN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(2..793)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(794..862)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(863..965)
                     /label="cat promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          951..970
                     /label="Copy of SeqR-2"
                     /note="sequence: cttacgtgccgatcaagtca"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     terminator      966..997
                     /label="tonB terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1018..1043
                     /label="Copy of PCR-Col_Domestication_Rv"
                     /note="sequence: atgatttaaatggtcagtattgagcg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     promoter        1162..1465
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        1466..1669
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1721..1740
                     /label="GLuc-F1"
                     /note="sequence: ACCGTCAGATCCGCTAGAGA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1750..1766)
                     /label="p7_R"
                     /note="sequence: atagatCGTCTCtgctgcccatggtggcgg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      1752..1761
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             1767..2318
                     /label="hGLuc"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2300..2318)
                     /label="IGLuc_p8_ER"
                     /note="sequence: atagatCGTCTCaGCCTGAGTCACCACCGGCCCCCTTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2300..2318)
                     /label="p7-GLuc_Rv"
                     /note="sequence: atagatGGTCTCaGCCTGAGTCACCACCGGCCCCCTTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2335..2359
                     /label="p7_F"
                     /note="sequence: atagatCGTCTCaAGGCTAAccggactctagaacatccctacagg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2353..2374)
                     /label="INV-mCherry_P10-ESP3I-Rv"
                     /note="sequence: atagatCGTCTCaagcacgaggatatcacctgtag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2375..2375
                     /label="appended G"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2376..2411
                     /label="RfxCas13d DR36"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          2396..2420
                     /label="IGLuc_p11_EF"
                     /note="sequence: atagatCGTCTCaGGTAGGTCGGGGTTTGAAACGGTAacttg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2412..2440
                     /label="INV-mCherry_P10-ESP3I-Fw"
                     /note="sequence: atagatCGTCTCaGGTAacttgtttattgcagcttataatgg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2417..2536
                     /label="SV40 poly(A)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2452..2471)
                     /label="Copy of Genewiz Seq SV40pA_R"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     enhancer        2743..3046
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        3047..3250
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        3295..3313
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(3295..3313)
                     /label="T7-R"
                     /note="sequence: CCTATAGTGAGTCGTATTA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3326..4972
                     /label="Red Firefly Luciferase"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          complement(3705..3724)
                     /label="RDF-R1"
                     /note="sequence: CTTCTTGCTGCTGAACACGA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(4494..4511)
                     /label="RFL-R2"
                     /note="sequence: CCTTCACGCACACTTCGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    4998..5109
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      5115..5250
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(5318..5338)
                     /label="Copy of SeqF-2"
                     /note="sequence: gaaggtgagccagtgagttga"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     terminator      5328..5357
                     /label="T3Te terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(5348..5372)
                     /label="Copy of PCR-Col_Domestication_Fw"
                     /note="sequence: cctttgattttctaccgaagaaagg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     rep_origin      5379..5966
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      5978..6005
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 attaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
       61 tagttgcctg actccccgtc gtgtagataa ctacgatgcg ggagggctta ccatctggcc
      121 ccagtgctgc aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa
      181 accagccagc cgggagggcc gagcgcagaa gtgatcctgc aactttatcc gcctccatcc
      241 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
      301 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
      361 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
      421 cggttagctc cttcggtcct ccgatcgttg ccagaagtaa gttggccgca gtgttatcac
      481 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgtg agatgctttt
      541 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
      601 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
      661 tcatcattgg aaaacgttct tcggggcgta aactctcaag gatcttaccg ctgttgagat
      721 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
      781 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
      841 cacggaaatg ttgaatactc attttagctt ccttagctcc tgaaaatctc gataactcaa
      901 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
      961 gatcaagtca aaagcctccg gtcggaggct tttgactttc tgctatggag gtcaggtatg
     1021 atttaaatgg tcagtattga gcgatatcta gagaattcgt ataggtcttg tattcagtct
     1081 cgcactttag acatggtctt ctgctgcccg tggtcctcta ttcgtcggat gttctgtcga
     1141 ttcttcctgt ctatctggac tcgttacata acttacggta aatggcccgc ctggctgacc
     1201 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1261 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1321 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1381 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1441 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1501 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     1561 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     1621 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctG GACttagcct
     1681 ttagtgaacc gtcagaatta attcagatcg atctaccaga accgtcagat ccgctagaga
     1741 ttacgccaac cgccaccatg ggcagcGGAG TCAAAGTTCT GTTTGCCCTG ATCTGCATCG
     1801 CTGTGGCCGA GGCCAAGCCC ACCGAGAACA ACGAAGACTT CAACATCGTG GCCGTGGCCA
     1861 GCAACTTCGC GACCACGGAT CTCGATGCTG ACCGCGGGAA GTTGCCCGGC AAGAAGCTGC
     1921 CGCTGGAGGT GCTCAAAGAG ATGGAAGCCA ATGCCCGGAA AGCTGGCTGC ACCAGGGGCT
     1981 GTCTGATCTG CCTGTCCCAC ATCAAGTGCA CGCCCAAGAT GAAGAAGTTC ATCCCAGGAC
     2041 GCTGCCACAC CTACGAAGGC GACAAAGAGT CCGCACAGGG CGGCATAGGC GAGGCGATCG
     2101 TCGACATTCC TGAGATTCCT GGGTTCAAGG ACTTGGAGCC CATGGAGCAG TTCATCGCAC
     2161 AGGTCGATCT GTGTGTGGAC TGCACAACTG GCTGCCTCAA AGGGCTTGCC AACGTGCAGT
     2221 GTTCTGACCT GCTCAAGAAG TGGCTGCCGC AACGCTGTGC GACCTTTGCC AGCAAGATCC
     2281 AGGGCCAGGT GGACAAGATC AAGGGGGCCG GTGGTGACAG GCtaataaca gcttccggac
     2341 tctagaacat ccctacaggt gatatcctcg tgctgCAAGT AAACCCCTAC CAACTGGTCG
     2401 GGGTTTGAAA CGGTAacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2461 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2521 tcatcaatgt atcttatcat gtctgtcgtc tatgatgagg atgttggtgg agagcatgtg
     2581 gaggaagtgg atagggaagg ttgtagagta gatccggttg aagtgatgag gataggagga
     2641 ggcgaatctt gtattcagtc tcgcacttta gacatggtct tctgctgccc gtggtcctct
     2701 attcgtcgga tgttctgtcg attcttcctg tctatctgca cgCGTTACAT AACTTACGGT
     2761 AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA
     2821 TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG
     2881 GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA
     2941 CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT
     3001 TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG
     3061 GCAGTACATC AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC
     3121 CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG
     3181 TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
     3241 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
     3301 GACTCACTAT AGGGGATCCG CCACCATGGA AAATATGGAA AACGACGAGA ACATCGTGGT
     3361 GGGCCCCAAG CCCTTCTACC CCATCGAGGA AGGCAGCGCC GGCACCCAGC TGCGGAAGTA
     3421 CATGGAAAGA TACGCCAAGC TGGGCGCCAT TGCCTTCACC AACGCCGTGA CCGGCGTGGA
     3481 CTACAGCTAC GCCGAGTACC TGGAAAAGAG CTGCTGCCTG GGCAAGGCTC TGCAGAACTA
     3541 CGGCCTGGTG GTGGACGGCC GGATCGCCCT GTGCAGCGAG AACTGCGAGG AATTCTTCAT
     3601 CCCCGTGATC GCCGGCCTGT TCATCGGCGT GGGCGTGGCT CCCACCAACG AGATCTACAC
     3661 CCTGCGGGAG CTGGTGCACA GCCTGGGCAT CAGCAAGCCC ACCATCGTGT TCAGCAGCAA
     3721 GAAGGGCCTG GACAAAGTCA TCACCGTGCA GAAAACCGTG ACCACCATCA AGACCATCGT
     3781 GATCCTGGAC AGCAAGGTGG ACTACCGGGG CTACCAGTGC CTGGACACCT TCATCAAGCG
     3841 GAACACCCCC CCTGGCTTCC AGGCCAGCAG CTTCAAGACC GTGGAGGTGG ACCGGAAAGA
     3901 ACAGGTGGCC CTGATCATGA ACAGCAGCGG CAGCACCGGC CTGCCCAAGG GCGTGCAGCT
     3961 GACCCACGAG AACACCGTGA CCCGGTTCAG CCACGCCAGG GACCCCATCT ACGGCAACCA
     4021 GGTGTCCCCC GGCACCGCCG TGCTGACCGT GGTGCCCTTC CACCACGGCT TCGGCATGTT
     4081 CACCACCCTG GGCTACCTGA TCTGCGGCTT CCGGGTGGTG ATGCTGACCA AGTTCGACGA
     4141 GGAAACCTTC CTGAAAACCC TGCAGGACTA CAAGTGCACC TACGTGATTC TGGTGCCCAC
     4201 CCTGTTCGCC ATCCTGAACA AGAGCGAGCT GCTGAACAAG TACGACCTGA GCAACCTGGT
     4261 GGAGATCGCC AGCGGCGGAG CCCCCCTGAG CAAAGAAGTG GGAGAGGCCG TCGCCAGGCG
     4321 GTTCAATCTG CCCGGCGTGC GGCAGGGCTA CGGCCTGACC GAGACAACCA GCGCCATCAT
     4381 CATCACCCCC GAGGGCGACG ACAAGCCTGG AGCCAGCGGC AAGGTGGTGC CCCTGTTCAA
     4441 GGCCAAAGTG ATCGACCTGG ACACCAAGAA GAGCCTGGGC CCCAACAGAC GGGGCGAAGT
     4501 GTGCGTGAAG GGCCCCATGC TGATGAAGGG CTACGTGAAC AACCCCGAGG CCACCAAAGA
     4561 GCTGATCGAC GAAGAGGGCT GGCTGCACAC CGGCGACATC GGCTACTACG ACGAAGAGAA
     4621 GCACTTCTTC ATCGTGGACC GGCTGAAGAG CCTGATCAAG TACAAGGGCT ATCAGGTGCC
     4681 CCCTGCCGAG CTGGAAAGCG TCCTGCTGCA GCACCCCAGC ATCTTCGACG CCGGCGTGGC
     4741 CGGGGTGCCA GATCCTGTGG CCGGCGAGCT GCCTGGCGCC GTGGTGGTGC TGGAATCCGG
     4801 CAAGAACATG ACCGAGAAAG AAGTGATGGA CTACGTCGCC AGCCAGGTGT CCAACGCCAA
     4861 GCGGCTGAGA GGCGGCGTGA GATTCGTGGA CGAAGTGCCA AAGGGCCTGA CCGGCAAGAT
     4921 CGACGGCAGG GCCATCCGGG AGATCCTGAA GAAACCCGTG GCCAAGATGT GATGAGCGGC
     4981 CGCAAAATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC
     5041 CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA
     5101 AATTGCATCA actgatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5161 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     5221 agaagtagtg aggaggcttt tttggaggcc acgataacga attcaagctt gatatcattc
     5281 aggacgagcc tcagactcca gcgtaactgg actgcaatca actcactggc tcaccttcac
     5341 gggtgggcct ttcttcggta gaaaatcaaa ggatcttctt gagatccttt ttttctgcgc
     5401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5461 caagagctac caactctttt tccgaggtaa ctggcttcag cagagcgcag ataccaaata
     5521 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5581 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5641 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5701 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5761 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5821 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5881 atctttatag tcctgtcggg tttcgccacc tctgacttga gcatcgattt ttgtgatgct
     5941 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc agaaaggccc acccgaaggt
     6001 gagccaggtg attacatttg ggccctc
//