LOCUS       AGG1829_OpIE2-Barnase-S 3751 bp ds-DNA     circular     27-AUG-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..785
                     /label="OpIE-2 promoter"
     misc_signal     780..789
                     /label="Kozak sequence-002_"
     misc_feature    786..1289
                     /label="barnase"
     misc_feature    1008..1124
                     /label="synthetic intron"
     misc_feature    1245..1286
                     /label="V5 epitope (aa sequence)"
     terminator      1296..1525
                     /label="SV40 3'UTR"
ORIGIN
        1 CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG
       61 TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT
      121 GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC
      181 GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC
      241 ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC
      301 GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT
      361 GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA
      421 GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCGCG TGACCGGACA CGAGGCGCCC
      481 GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG
      541 CATCTGTTCC TGCAGAAGTT GGTCGTGAGG CACTGGGCAG GTAAGTATCA AGGTTACAAG
      601 ACAGGTTTAA GGAGACCAAT AGAAACTGGG CTTGTCGAGA CAGAGAAGAC TCTTGCGTTT
      661 CTGATAGGCA CCTATTGGTC TTACTGACAT CCACTTTGCC TTTCTCTCCA CAGGTGTCCA
      721 CTCCCAGTTC AATTACAGCT CTTAAGGCTA GAGTACTTAA TACGACTCAC TATAGGCTAG
      781 CCACCATGGC CCAGGTTATC AACACCTTCG ATGGAGTGGC CGATTACCTG CAGACCTACC
      841 ACAAGCTGCC GGATAACTAC ATCACCAAGT CGGAAGCCCA GGCCCTGGGA TGGGTGGCCT
      901 CGAAGGGAAA CCTGGCCGAT GTGGCCCCGG GAAAGTCGAT CGGAGGAGAT ATCTTCTCGA
      961 ACCGTGAAGG AAAGCTGCCG GGAAAGTCGG GACGTACCTG GCGTGAAGTA AGAGGAGATC
     1021 TTGACTAAGT AGATTTATGG CGCAACGCTA TCACAGGAAC ATAACTGACA TAATCTAGAC
     1081 GCGTTACTAA CTGGCACCTC TTCTTTTTTT TTGATATCCT GCAGGCCGAT ATCAACTACA
     1141 CCTCGGGATT CCGTAACTCG GATCGTATCC TGTACTCGTC GGATTGGCTG ATCTACAAGA
     1201 CCACCGATCA CTACCAGACC TTCACCAAGA TCCGTGGAGC CGGAGGAAAG CCGATCCCGA
     1261 ACCCGCTGCT GGGACTGGAT TCGACCTAAA ctagttgatc ataatcagcc ataccacatt
     1321 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     1381 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     1441 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     1501 gtccaaactc atcaatgtat cttacCTAGT AGGCTAGGTG GAGGCTCAGT GATGATAAGT
     1561 CTGCGATGGT GGATGCATGT GTCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
     1621 CTCAGAGGGC ACAATCCTAT TCCGCGCTAT CCGACAATCT CCAAGACATT AGGTGGAGTT
     1681 CAGTTCGGCG TATGGCATAT GTCGCTGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
     1741 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG
     1801 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
     1861 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
     1921 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT
     1981 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
     2041 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
     2101 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
     2161 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG
     2221 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
     2281 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
     2341 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
     2401 TATTCAACAA AGCCGCCGTC CCGTCAAGTC AGCGTAAATG GGTAGGGGGC TTCAAATCGT
     2461 CCTCGTGATA CCAATTCGGA GCCTGCTTTT TTGTACAAAC TTGTTGATAA TGGCAATTCA
     2521 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2581 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2641 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2701 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAGCC ACGCTCACCG
     2761 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2821 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2881 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2941 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     3001 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     3061 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     3121 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     3181 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3241 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3301 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3361 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3421 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3481 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3541 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC AGATACCTGA
     3601 AACAAAACCC ATCGTACGGC CAAGGAAGTC TCCAATAACT GTGATCCACC ACAAGCGCCA
     3661 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC ATGCATAATC CGCACGCATC
     3721 TGGAATAAGG AAGTGCCATT CCGCCTGACC T
//