LOCUS       AGG1767_loxPUb-Bs-lox- 11236 bp ds-DNA     circular     27-AUG-2024
DEFINITION  .
COMMENT     BarstarA-FLAG-H6
            MKKAVINGEQIRSISDLHQTLKKELALPEYYGENLDALWDALTGWVEYPLVLEWRQFEQS
            KQLTENGAESVLQVFREAKAEGADITIILS-gag-DYKDDDDK-sgag-HHHHHH [have added
            an epitope tag and His-tag: gag-DYKDDDDK-sgag-HHHHHH on C-terminus.
            Should be able to go on either end. 111aa/12.6kDa with this tag.
            May be able to mutate both cysteines to alanine (I think C41A/C83A)
            - this is barstar A, “functionally, barstar A is nearly the equal
            of the wild-type barstar” https://pdfs.semanticscholar.org/7e2c/3b8
            a94e7ac230790d02aa01bcde5b41cc474.pdf citing Hartley, R. W. (1993)
            Biochemistry 32, 5978-5984, even though Kd reduced from 6x10-14 to
            2 x 10-13. Tyr29, Tyr 30,Gly31, 33,35,36,39 all involved in binding
            H102 of barnase. Barnase-V5 M[la1] AQVINTFDGVADYLQTYHKLPDNYITKSEAQA
            LGWVASKGNLADVAPGKSIGGDIFSNREGKLPGKSGRTWRE A[SB2]
            DINYTSGFRNSDRILYSSDWLIYKTTDHYQTFTKIR-gag-GKPIPNPLLGLDST [use same
            nucleotide sequence as for nuclease-dead versions, except obviously
            H102H (not A), removing protease linker and adding M, also added
            C-terminal V5 epitope tag] Glu-73, Arg-87 and His-102 all crucial,
            so I’ll insert intron at Arg-83 [la1]Added M - bacterial version
            has a leader peptide. [SB2]Synthetic intron here  want the TEV
            version but not the AF
FEATURES             Location/Qualifiers
     misc_recomb     complement(1615..1927)
                     /label="piggyBac 5' flank"
     misc_binding    1933..1982
                     /label="attP50 site"
     misc_recomb     1983..2016
                     /label="LoxN"
     misc_feature    2032..3422
                     /label="PUb promoter"
     misc_feature    3424..3759
                     /label="BarStarA aa seq"
     misc_feature    3703..3726
                     /label="Flag-tag"
     CDS             3739..3756
                     /label="6x His Tag"
     3'UTR           3777..4442
                     /label="P10 3'UTR"
     misc_feature    4445..5646
                     /label="Hr5-IE1 promoter"
     intron          5691..5760
                     /label="Alcohol DeHydrogenase Intron"
     CDS             5787..6545
                     /label="DsRed"
     misc_binding    5796..5822
                     /label="nls"
     misc_binding    6510..6545
                     /label="nls"
     misc_feature    6568..7349
                     /label="K10\3'UTR"
     misc_recomb     7358..7391
                     /label="LoxP"
     misc_recomb     7402..7435
                     /label="LoxN"
     misc_feature    complement(7477..7747)
                     /label="tetracycline response element"
     misc_feature    7766..7895
                     /label="Hsp70 minimal promoter"
     misc_feature    7918..7980
                     /label="scraps intron"
     misc_feature    8010..8642
                     /label="Barnase"
     misc_feature    8013..8054
                     /label="V5 epitope (aa sequence)"
     misc_feature    8346..8462
                     /label="synth intron"
     terminator      complement(8649..8877)
                     /label="SV40 3'UTR"
     misc_feature    complement(8878..9570)
                     /label="ZsYellow"
     promoter        complement(9571..9799)
                     /label="3xP3 Promoter"
     misc_recomb     9806..9839
                     /label="LoxP"
     misc_recomb     9850..10098
                     /label="piggyBac 3' flank"
     misc_feature    10115..1596
                     /label="pUC57 backbone"
ORIGIN
        1 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
       61 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
      121 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
      181 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
      241 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
      301 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
      361 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
      421 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
      481 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
      541 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
      601 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      661 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      721 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      781 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      841 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      901 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      961 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1021 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1081 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     1141 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     1201 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     1261 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     1321 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1501 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctTG CATGCAGGCC
     1561 TCTGCAGTCG ACGGGCCCGG GATCCGATAT CCCAatTATA GGGAGAGCGG CCGCttaacc
     1621 ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc
     1681 taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc
     1741 gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga
     1801 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
     1861 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
     1921 agatatccct agagtagtgc cccaactggg gtaacctttg agttctctca gttgggggcg
     1981 taataacttc gtataGtATA CcTtatacga agttatGCTA GCaGCGATCG CTATCTTTAC
     2041 ATGTAGCTTG TGCATTGAAT CCAATTATAA TTTGCCTTGG CACCAGCTGA GCCAGACAAG
     2101 AAAGAAAGCT TCCCAGAAGT ATATCGATTT AGAAGGGTTG ACGTCACTTG CTGACTGCAC
     2161 TAATACAGCA AATGATgCAA TTAGAATGAT TCAAGTGAAA TTCCCAAATT ACTGaTTTtT
     2221 CTCTGGATTT GGTTATCAGA TTACATTCGA AGCTAAGAtT AGCTACCGAA ATTGTCGATC
     2281 AAATCAGGAA ATCCTTTCTC TATCGAAAAA GGCATTCGCA CATCTTcCTC TcTATGCCAT
     2341 ATACACGAAg GGTAGGTACA TTGACGTCTT TGCCAGAAGT TGAACTGCAT CGTTCAAGGT
     2401 ACAGAATGAA CGACTAACAG ACACAAGCAC GTTTTGCTGT CCATTCAGAC ACAGGGATGG
     2461 TACCCATAtT CGATCGATaT AGAGCCATCC AACCGAACAG AGGTATATGT ATGAATGtAT
     2521 TGCtGAAATT TTCTAGAAGT ACAACCACCA CTACGaCAGT GTCTATAAAA CGCCCCTGCA
     2581 AAGGCgAAAC CAGCTCAATC GAATACGTTT CCTAGTGGAG TGAACATTAC GCGGcCCAAG
     2641 TAAGCAGTGC CAGTGCAAGT GAAGTGAAGT CTCTAGTGAA AAAGAGTGAT CCAATTAGCC
     2701 AGAGGAGAAA ATTTCAGAGT GAACAAAGCT TTaTTCAAAG GACAATTACT ATTAAATTgG
     2761 TGAAAGTGCA TTTCGGTGAA GGGAATCTTC TAGTGAAGGT AGGTAAATTA AtTGATGAAA
     2821 TTATAGCTAT GAGCGAAAAC TAGTTTGGTG AATGATTCCT TTGTCTTTGA ATGAGCAAAC
     2881 TATTTTCCAA GATGGCGACT ATTGAGCTTT GAGTGATTAG TGAAAATTTG CAACGCAGTT
     2941 TCATCATCAT TGATAAAACC CAATTGTGAT TCACaGCGAT AATCATATTT CGTTGAATCA
     3001 TCGCTaCTAA TTGAATTAAA TTTCTAGAAT AATAAGAATA ACGTATTTGC TCCGTCACAT
     3061 ATCTAAAATA AATATTTTGA TGGTAATTAC CCATTAAGGT AATATTAACA CATATCGAGA
     3121 AAAACCTTGA GGAAATCGTG AAAACTTGAA GATACGCAAT TTCaAAACTA CGTAGTTCAA
     3181 AGTCGAAAAC AAGTTAATTT TTCACTtAAA AGTAGGGCGT TGTTGTGACG TCATCACCTT
     3241 CAAGTGTATA TTTTTCACTT GGCCTGCGAC TGCAAACGCA GACAAAGCAA AACAAGTTTA
     3301 AAACCTGTCG TGTCGTGCTC GAAGCCAAAG GCAATGAATC AATATCAAAT GAGAGTTTGC
     3361 ATTTCACAAC CAATTACTcA AGCGTTTCCT CGTTTCTTTT TCTGCTCAAC AGAGATTTCA
     3421 ACAatgaaga aggccgtgat caacggagaa cagatccgtt cgatctcgga tctgcaccag
     3481 accctgaaga aggaactggc cctgccggaa tactacggag aaaacctgga tgccctgtgg
     3541 gatgccctga ccggatgggt ggaatacccg ctggtgctgg aatggCGGca gttcgaacag
     3601 tcgaagcagc tgaccgaaaa cggagccgaa tcggtgctgc aggtgttccg tgaagccaag
     3661 gccgaaggag ccgatatcac catcatcctg tcgGGTgccg gaGACtacAA AgatGACgat
     3721 GACaagAGCG GCgccggaca cCATcaccac CATCATtaac tagctgaCAC TACGTGatga
     3781 atcgttttta aaataacaaa tcaattgttt tataatattc gtacgattct ttgattatgt
     3841 aataaaatgt gatcattagg aagattacga aaaatataaa aaatatgagt tctgtgtgta
     3901 taacaaatgc tgtaaacgcc acaattgtgt ttgttgcaaa taaacccatg attatttgat
     3961 taaaattgtt gttttctttg ttcatagaca atagtgtgtt ttgcctaaac gtgtactgca
     4021 taaactccat gcgagtgtat agcgagctag tggctaacgc ttgccccacc aaagtagatt
     4081 cgtcaaaatc ctcaatttca tcaccctcct ccaagtttaa catttggccg tcggaattaa
     4141 cttctaaaga tgccacataa tctaataaat gaaatagaga ttcaaacgtg gcgtcatcgt
     4201 ccgtttcgac catttccgaa aagaactcgg gcataaactc tatgatttct ctggacgtgg
     4261 tgttgtcgaa actctcaaag tacgcagtca ggaacgtgcg cgacatgtcg tcgggaaact
     4321 cgcgcggaaa catgttgttg taaccgaacg ggtcccatag cgccaaaacc aaatctgcca
     4381 gcgtcaatag aatgagcacg atgccgacaa tggagctggc ttggatagcg attcgagtta
     4441 acAagcttta cgagtagaat tctacgcgta aaacacaatc aagtatgagt cataatctga
     4501 tgtcatgttt tgtacacggc tcataaccga actggcttta cgagtagaat tctacttgta
     4561 atgcacgatc agtggatgat gtcatttgtt tttcaaatcg agatgatgtc atgttttgca
     4621 cacggctcat aaactcgctt tacgagtaga attctacgtg taacgcacga tcgattgatg
     4681 agtcatttgt tttgcaatat gatatcatac aatatgactc atttgttttt caaaaccgaa
     4741 cttgatttac gggtagaatt ctacttgtaa agcacaatca aaaagatgat gtcatttgtt
     4801 tttcaaaact gaactcgctt tacgagtaga attctacgtg taaaacacaa tcaagaaatg
     4861 atgtcatttg ttataaaaat aaaagctgat gtcatgtttt gcacatggct cataactaaa
     4921 ctcgctttac gggtagaatt ctacgcgtaa aacatgattg ataattaaat aattcatttg
     4981 caagctatac gttaaatcaa acggacgctc gaggttgcac aacactatta tcgatttgca
     5041 gttcgggaca taaatgttta aatatatcga tgtctttgtg atgcgcgcga catttttgta
     5101 ggttattgat aaaatgaacg gatacgttgc ccgacattat cattaaatcc ttggcgtaga
     5161 atttgtcggg tccattgtcc gtgtgcgcta gtagcatgcc cgtaacggac ctcgtacttt
     5221 tggcttcaaa ggttttgcgc acagacaaaa tgtgccacac ttgcagctct gcatgtgtgc
     5281 gcgttaccac aaatcccaac ggcgcagtgt acttgttgta tgcaaataaa tctcgataaa
     5341 ggcgcggcgc gcgaatgcag ctgatcacgt acgctcctcg tgttccgttc aaggacggtg
     5401 ttatcgacct cagattaatg tttatcggcc gactgttttc gtatccgctc accaaacgcg
     5461 tttttgcatt aacattgtat gtcggcggat gttctatatc taatttgaat aaataaacga
     5521 taaccgcgtt ggttttagag ggcataataa aagaaatatt gttatcgtgt tcgccattag
     5581 ggcagtataa attgacgttc atgttggata ttgtttcagt tgcaagttga cactggcggc
     5641 gacaagcaat tggtacccgg gtaggatcct agtgaattcc taatctggcg gtaagttgat
     5701 caaaggaaac gcaaagtttt caagaaaaaa caaaactaat ttgatttata acacctttag
     5761 aaagcgaagt tgagattcag gccaccatgg gagatcccac cccacccaag aagaagcgca
     5821 aagctagcat ggcctcctcc gagaacgtca tcaccgagtt catgcgcttc aaggttcgca
     5881 tggagggcac cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     5941 acgagggcca caacaccgtg aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     6001 gggacatcct gtccccccag ttccagtacg gctccaaggt ctacgtgaag caccccgccg
     6061 acatccccga ctacaagaag ctgtccttcc cggaaggatt caagtgggag cgcgtgatga
     6121 acttcgagga cggcggcgtg gctaccgtga cccaggactc ctccctgcaa gacggctgct
     6181 tcatctacaa ggttaagttc atcggcgtga acttcccctc cgacggcccc gtgatgcaga
     6241 agaagacaat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     6301 agggcgagac ccacaaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     6361 agtccatcta catggccaag aagcccgtgc agctgcccgg ctactactac gtggacgcca
     6421 agctggacat cacctcccac aacgaggact acaccatcgt ggagcagtac gagcgcaccg
     6481 agggccgcca ccacctgttc ctgagatctc gacccaagaa aaagcggaag gtggaggacc
     6541 cgtaagatcc accggatcta gataactgga gcttgataac attataccta aacccatggt
     6601 caagagtaaa catttctgcc tttgaagttg agaacacaat taagcatccc ctggttaaac
     6661 ctgacattca tacttgttaa tagcgccata aacatagcac caatttcgaa gaaatcagtt
     6721 aaaagcaatt agcaattagc aattagcaat aactctgctg acttcaaaac gagaagagtt
     6781 gcaagtattt gtaaggcaca gtttatagac caccgacggc tcattagggc tcgtcatgta
     6841 actaagcgcg gtgaaaccca attgaacata tagtggaatt attattatca atggggaaga
     6901 tttaaccctc aggtagcaaa gtaatttaat tgcaaataga gagtcctaag actaaataat
     6961 atatttaaaa atctggccct ttgaccttgc ttgtcaggtg catttgggtt caatcgtaag
     7021 ttgcttctat ataaacactt tccccatccc cgcaataatg aagaataccg cagaataaag
     7081 agagatttgc aacaaaaaat aaaggcattg cgaaaacttt ttatggggga tcattacact
     7141 cgggcctacg gttacaattc ccagccactt aagcgacaag tttggccaac aatccatcta
     7201 atagctaata gcgcaatcac tggtaatcgc aagagtatat aggcaataga acccatggat
     7261 ttgaccaaag gtaaccgaga caatggagaa gcaagaggat ttcaaactga acacccacag
     7321 tactgtgtac taccactggc gcgtttgggg gCCGGCCata acttcgtata atgtatgcta
     7381 tacgaagtta tGACACGGAA Aataacttcg tataGtATAC cTtatacgaa gttatGGCGC
     7441 GCCCTCGAGc gcgcctcgga attcgacttt cacttttctc tatcactgat agggagtggt
     7501 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt
     7561 tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc actgataggg
     7621 agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt
     7681 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg
     7741 atagggagtg gtaaactcga aaacgagcgc cggagtataa atagaggcgc ttcgtctacg
     7801 gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa
     7861 taaacaagcg cagctgaaca agctaaacaa tctgcggtac cctggcgtct aattggggta
     7921 agttttcccg ttcttttctg ggttcttccc ttttgctcat ccttgctgca ctaccttcag
     7981 gtgcaagtaa agcccGCGGt gggACTAGCa tgggaaagcc gatcccgaac ccgctgctgg
     8041 gactggattc gaccGGTAGC ggagccGGCA GTGGTgccGG CTCCggagcc GGTtcgggag
     8101 ccGGCAGCGG TgccGGCTCC ggaaccgccc agGTTatcaa caccttcgat ggagtggccg
     8161 attacctgca gacctaccac aagctgccgg ataactacat caccaagtcg gaagcccagg
     8221 ccctgggatg ggtggcctcg aagggaaacc tggccgatgt ggccccggga aagtcgatcg
     8281 gaggagatat cttctcgaac cgtgaaggaa agctgccggg aaagtcggga cgtacctggc
     8341 gtgaaGTAAG AGGAGATCTT GACTAAGTAG ATTTATGGCG CAACGCTATC ACAGGAACAT
     8401 AACTGACATA ATCTAGACGC GTTACTAACT GGCACCTCTT CTTTTTTTTT GATATCCTGC
     8461 AGgccgatat caactacacc tcgggattcc gtaactcgga tcgtatcctg tactcgtcgG
     8521 GTggaAGCGG CAGTgaaaac ctgtacttcc agggatcggg atcgggaacc ggagccggat
     8581 cgtcggattg gctgatctac aagaccaccg atgcctacca gaccttcacc aagatccgtt
     8641 aaCCTAGTta agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     8701 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     8761 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     8821 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcaggc
     8881 cagggcgctg gggaaggcga tggcgtgctc ggtcagctgc cacttctggt tcttggcgtc
     8941 gctccggtcc tcccgcagca gcttgtgctg gatgaagtgc cactcgggca tcttgctggg
     9001 cacgctcttg gccttgtaca cggtgtcgaa ctggcaccgg taccggccgc cgtccttcag
     9061 cagcaggtac atgctgacat cgcccttcag gatgccctgc ttaggcacgg gcatgatctt
     9121 ctcgcagctg gcctcccagt tggtggtcat cttcttcatc acggggccgt cggcggggaa
     9181 gttcacgccg ttgaagatgc tcttgtggta gatgcagttc tccttcacgc tcacggtgat
     9241 gtccacgtta cagatgcaca cggcgccgtc ctcgaacagg aagctccggc cccaggtgta
     9301 gccggcgggg cagctgttct tgaagtagtc cacgatgtcc tgggggtact cggtgaagat
     9361 ccggtcgccg tacttgaagc cggcgctcag gatgtcctcg ctgaagggca gggggccgcc
     9421 ctcgatcacg cacaggttga tggtctgctt gcccttgaag gggtagccga tgccctcgcc
     9481 ggtgatcacg aacttgtggc cgttcacgca gccctccatg tggtacttca tggtcatctc
     9541 ctccttcagg ccgtgcttgc tgtgggccat tcgactctag cggtaccccg attgtttagc
     9601 ttgttcagct gcgcttgttt atttgcttag ctttcgctta gcgacgtgtt cactttgctt
     9661 gtttgaattg aattgtcgct ccgtagacga agcgcctcta tttatactcc ggcggtcgag
     9721 ggttcgaaat cgataagctt ggatcctaat tgaattagct ctaattgaat tagtctctaa
     9781 ttgaattaga tccccgggcC CTAGGataac ttcgtataat gtatgctata cgaagttatg
     9841 ACTCACGTTa attcgataaa agttttgtta ctttatagaa gaaattttga gtttttgttt
     9901 ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa
     9961 gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc gatatacaga
    10021 ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga
    10081 ttatctttct agggttaaCC ATGGAAAATC GATGatctCg aCgcattcgc gaAgtaccga
    10141 TctcCaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
    10201 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
    10261 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
    10321 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
    10381 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
    10441 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
    10501 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
    10561 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
    10621 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10681 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10741 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10801 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10861 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    10921 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    10981 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    11041 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    11101 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    11161 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    11221 gccttgatcg ttggga
//