LOCUS       pLV-puro-EF1A-Tag100-hASB8        9400 bp    DNA     circular SYN 18-AUG-2025
DEFINITION  .
ACCESSION   VB200306-1537ajt
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Promoter        1..229
                     /application_notes="Strong promoter; drives transcription
                     of viral RNA in packaging cells."
                     /description="Rous sarcoma virus enhancer/promoter"
                     /full_name="Rous sarcoma virus (RSV) enhancer/promoter"
                     /note="color: #ef6cdf; direction: RIGHT"
                     /official_designation="RSV"
                     /uuid="3f2ddca7f0cb4015b6abcec8a25c834e"
                     /label="RSV  promoter"
     LTR             230..410
                     /application_notes="Allows transcription of viral RNA and
                     its packaging into virus."
                     /description="Truncated HIV-1 5' long terminal repeat"
                     /full_name="HIV-1 truncated 5' LTR"
                     /note="color: #c54b7c; direction: RIGHT"
                     /official_designation="Delta5' LTR"
                     /uuid="44156a3083604321be44066133e8ecb9"
                     /modified_by="u0063854"
                     /label="5' LTR-ΔU3"
     motif           1075..1308
                     /application_notes="Rev protein binding site that allows
                     Rev-dependent nuclear export of viral RNA during viral
                     packaging."
                     /description="HIV-1 Rev response element"
                     /full_name="HIV-1 Rev response element"
                     /note="color: #fd3434; direction: RIGHT"
                     /official_designation="RRE"
                     /uuid="df07099e49674cf3aba3661b54e28bd7"
                     /modified_by="u0063854"
                     /label="RRE"
     motif           1803..1920
                     /application_notes="Facilitates the nuclear import of
                     HIV-1 cDNA through a central DNA flap."
                     /description="Central polypurine tract"
                     /full_name="Central polypurine tract"
                     /note="color: #46c6ef; direction: RIGHT"
                     /official_designation="cPPT"
                     /uuid="f7cb29985f9f41a7a5924d3462266f42"
                     /modified_by="u0063854"
                     /label="cPPT"
     Promoter        1959..3137
                     /application_notes="Strong promoter."
                     /description="Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /full_name="Human eukaryotic translation elongation
                     factor1 alpha1 promoter"
                     /note="color: #eda481; direction: RIGHT"
                     /official_designation="EF1A"
                     /uuid="69c052aac71a4f49bab5b7ae1f46ace8"
                     /label="EF1A"
     domain          3171..3206
                     /created_by="User"
                     /Source_Sequence="referenceToDocument:
                     urn:local:.:123-iiaynmu@52.20#0:[3953,3989)#86FB1F3BE64B6D
                     0928875B6AC438057F label: 15. pLentiGuide-Procode-Tag100:
                     3954->3989"
                     /label="Tag-100"
     CDS             3207..4073
                     /application_notes="/label=""3xFLAG/hASB8[NM_024095.5]"
                     /note="color: #028a02; direction: RIGHT"
                     /official_designation="hASB8[NM_024095.5]"
                     /uuid="ce7d5db402244d3884b39c391a772808"
                     /modified_by="u0063854"
                     /label="ASB8"
     motif           4112..4709
                     /application_notes="Enhances virus stability in packaging
                     cells, leading to higher titer of packaged virus; enhances
                     higher expression of transgenes."
                     /description="Woodchuck hepatitis virus
                     posttranscriptional regulatory element"
                     /full_name="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #e4b930; direction: RIGHT"
                     /official_designation="WPRE"
                     /uuid="0aedff8308474b369c622433538d7836"
                     /modified_by="u0063854"
                     /label="WPRE"
     Promoter        4728..5237
                     /application_notes="Medium-strength promoter."
                     /description="Mouse phosphoglycerate kinase 1 promoter"
                     /full_name="Mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #7a00b5; direction: RIGHT"
                     /official_designation="mPGK promoter"
                     /uuid="bce93795b5fd4173b8b9abc832edad80"
                     /label="mPGK promoter"
     CDS             5250..5849
                     /application_notes="Allows cells to be resistant to
                     puromycin."
                     /description="Puromycin resistance gene"
                     /full_name="Puromycin resistance gene"
                     /note="color: #05696d; direction: RIGHT"
                     /official_designation="Puro"
                     /uuid="39d7413208494d9291d10b21b13ca44c"
                     /modified_by="u0063854"
                     /label="Puro"
     LTR             5920..6154
                     /application_notes="Allows packaging of viral RNA into
                     virus; self-inactivates the 5' LTR by a copying mechanism
                     during viral genome integration; contains polyadenylation
                     signal for transcription termination."
                     /description="Truncated HIV-1 3' long terminal repeat"
                     /full_name="HIV-1 truncated 3' LTR"
                     /note="color: #f54c6b; direction: RIGHT"
                     /official_designation="DeltaU3/3' LTR"
                     /uuid="c958755414c34372b909e20e81ebcef6"
                     /label="3' LTR-ΔU3"
     PolyA_signal    6227..6361
                     /application_notes="Allows transcription termination and
                     polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /description="Simian virus 40 early polyadenylation
                     signal"
                     /full_name="SV40 early polyadenation signal"
                     /note="color: #944603; direction: RIGHT"
                     /official_designation="SV40 early pA"
                     /uuid="2fcb13a62e704d8095b188d9625a6a94"
                     /label="SV40 early pA"
     motif           7315..8175
                     /application_notes="Allows E. coli to be resistant to
                     ampiciIIin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /note="color: #ce0084; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="6a7994dc58024d80b36e4a85ad2e1d99"
                     /modified_by="u0063854"
                     /label="AmpiciIIin"
     Rep_origin      8346..8934
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /note="color: #6d3ed8; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="64a8cb3def9f49deb6752795c2d893a8"
                     /label="pUC ori"
ORIGIN      
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gaagaaacag
     3181 cgaggttcca gcccggttac cggagtatga gttccagtat gtggtatatt atgcagagca
     3241 ttcagagcaa atactctctc tccgagcgct taatccgaac aattgctgcc atccgttcct
     3301 tcccacatga taatgtagag gacctcatca gagggggagc agatgtgaac tgcactcatg
     3361 gcacactgaa gcccttgcac tgtgcctgta tggtgtcaga tgctgactgt gtggagttac
     3421 ttctggaaaa aggagccgag gtgaatgccc tggatgggta taaccgaaca gccctccact
     3481 atgcagcaga gaaagatgag gcttgtgtgg aggtcctatt ggagtatggt gcaaacccca
     3541 atgctttgga tggcaacaga gataccccac ttcactgggc agcctttaag aacaatgctg
     3601 agtgtgtgcg ggctctccta gagagcgggg cctctgtcaa tgccctggat tacaacaatg
     3661 atacaccgct cagctgggct gccatgaagg gaaatcttga gagtgtcagc atccttctgg
     3721 attatggcgc agaggtcaga gtcatcaacc taataggcca gacacccatc tcccgcctgg
     3781 tggctctgct agtcagggga cttggaacag agaaagagga ctcttgcttt gagctcctcc
     3841 acagagctgt tggacacttt gaattgagga aaaatggcac catgccacga gaggtggcca
     3901 gagacccgca gctatgtgaa aaactgactg ttctgtgctc agctccagga actctaaaaa
     3961 cactcgctcg ctatgccgtg cgccgtagcc tgggactcca gtatctcccc gatgcagtga
     4021 agggccttcc actgccagct tctttgaagg aatacctgtt acttttagaa tagacccagc
     4081 tttcttgtac aaagtggtga taatcgaatt ccgataatca acctctggat tacaaaattt
     4141 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4201 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4261 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4321 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4381 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4441 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4501 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     4561 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4621 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4681 tttccctttg ggccgcctcc ccgcatcggg aattcccgcg gttcgaattc taccgggtag
     4741 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     4801 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     4861 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     4921 tccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     4981 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     5041 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     5101 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     5161 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     5221 tccgggcctt tcgacctcac gtggccacca tgaccgagta caagcccacg gtgcgcctcg
     5281 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     5341 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     5401 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     5461 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     5521 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     5581 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     5641 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     5701 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     5761 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     5821 ggtgcatgac ccgcaagccc ggtgcctgag gtacctttaa gaccaatgac ttacaaggca
     5881 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5941 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6001 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6061 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6121 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6181 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6241 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6301 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     6361 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6421 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6481 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     6541 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     6601 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcaccagctg
     6661 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6721 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6781 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6841 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6901 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6961 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7021 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7081 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7141 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     7201 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7261 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7321 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7381 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7441 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7501 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7561 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7621 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7681 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7741 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7801 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7861 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7921 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7981 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8041 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8101 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8161 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8221 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8281 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8341 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8401 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8461 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8521 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8581 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8641 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8701 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8761 gaagagagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8821 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8881 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8941 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9001 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9061 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9121 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     9181 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9241 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9301 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     9361 aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
