LOCUS       pLCKO-CMV-cMyc-UBC-IRES-Blasti        8033 bp    DNA     circular UNA 18-AUG-2025
DEFINITION  .
ACCESSION   urn.local...32n-jiv05w6
VERSION     urn.local...32n-jiv05w6
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     enhancer        1..379
                     /note="human cytomegalovirus immediate early enhancer"
                     /modified_by="u0063854"
                     /label="CMV enhancer"
     promoter        380..583
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     promoter        628..646
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             688..690
                     /created_by="u0063854"
                     /modified_by="u0063854"
                     /label
     domain          691..720
                     /created_by="User"
                     /label="cMyc-Tag"
     CDS             721..948
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /modified_by="u0063854"
                     /label="UBC"
     domain          969..1553
                     /created_by="u0063854"
                     /label="IRES"
     CDS             1586..1984
                     /created_by="User"
                     /modified_by="u0063854"
                     /label="Blasticidin"
     domain          2002..2577
                     /Source="Geneious"
                     /label="WPRE"
     domain          2643..2658
                     /Source="Geneious"
                     /label="cPPT"
     domain          2660..2712
                     /Source="Geneious"
                     /label="delta_U3"
     domain          2713..2893
                     /Source="Geneious"
                     /label="HIV-1_5_LTR"
     domain          2974..3093
                     /Source="Geneious"
                     /label="SV40_PA_terminator"
     domain          3111..3209
                     /Source="Geneious"
                     /label="SV40_origin"
     domain          3325..3473
                     /Source="Geneious"
                     /label="lacZ_a"
     domain          3491..3797
                     /Source="Geneious"
                     /label="f1_origin"
     domain          4060..4919
                     /Source="Geneious"
                     /label="Ampicillin"
     domain          5074..5693
                     /Source="Geneious"
                     /label="pBR322_origin"
     domain          6374..6554
                     /Source="Geneious"
                     /label="HIV-1_5_LTR"
     domain          6665..6709
                     /Source="Geneious"
                     /label="HIV-1_psi_pack"
     domain          7219..7452
                     /Source="Geneious"
                     /label="RRE"
     domain          7868..7985
                     /created_by="User"
                     /modified_by="User"
                     /label="central DNA flap"
     domain          7872..7887
                     /Source="Geneious"
                     /modified_by="User"
                     /label="cPPT"
     domain          7970..7981
                     /created_by="User"
                     /label="CTS"
ORIGIN      
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
      601 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
      661 ctagcgttta aacgggccct ctagaccatg gaacagaagc tgatctccga ggaggacctt
      721 cagatcttcg tgaagaccct gactggtaag accatcactc tcgaagtgga gccgagtgac
      781 accattgaga atgtcaaggc aaagatccaa gacaaggaag gcatccctcc tgaccagcag
      841 aggttgatct ttgctgggaa acagctggaa gatggacgca ccctgtctga ctacaacatc
      901 cagaaagagt ccaccctgca cctggtactc cgtctcagag gtgggtgagg taccggatcc
      961 ggaattccgc cccccccccc cccctccctc cccccccccc taacgttact ggccgaagcc
     1021 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     1081 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     1141 tttcccctct cgccaaggga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     1201 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     1261 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     1321 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     1381 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     1441 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     1501 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc
     1561 acaacccaca aggagacgac cttccatggc caagcctttg tctcaagaag aatccaccct
     1621 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     1681 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     1741 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     1801 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     1861 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     1921 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     1981 ctgaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2041 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2101 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2161 gtctctttat gaggagttgt ggcccgttgt caggcaacgc ggcgtggtgt gcactgtgtt
     2221 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2281 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2341 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     2401 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2461 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2521 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2581 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2641 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatttgctt
     2701 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     2761 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     2821 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     2881 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     2941 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3001 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3061 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3121 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3181 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3241 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     3301 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3361 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3421 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     3481 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3541 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3601 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3661 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3721 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3781 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3841 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3901 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     3961 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4021 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4081 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4141 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4201 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4261 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4321 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4381 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4441 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4501 gcttttttgc acaacatggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4861 tggatgaacg aaatagacag atcgctgaga taggcgcctc actgattaag cattggtaac
     4921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5701 tacggttcct ggtcttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5881 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5941 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6001 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6061 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6121 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     6181 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6241 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6301 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6361 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6421 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6481 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6541 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6601 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     6661 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     6721 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     6781 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     6841 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     6901 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     6961 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7021 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7081 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7141 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7201 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7261 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7321 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7381 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7441 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7501 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7561 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7621 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7681 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7741 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7801 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccagaga
     7861 gggcctattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     7921 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     7981 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gag
//
