LOCUS       pLCKO-CMV-cMyc-UBC_K7R-IRES-Blasti        8023 bp    DNA     circular UNA 18-AUG-2025
DEFINITION  .
ACCESSION   urn.local...7fx-jivhk4n
VERSION     urn.local...7fx-jivhk4n
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     enhancer        1..380
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        381..584
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     promoter        629..647
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             689..691
                     /created_by="u0063854"
                     /modified_by="u0063854"
                     /label
     domain          692..721
                     /created_by="User"
                     /label="cMyc-Tag"
     CDS             722..949
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /Source_Sequence="referenceToDocument:
                     urn:local:.:l-ic0v92b@8.4#0:[2292,2520)#AEA8BCE3B727F1B2DC
                     DAC074D0EC05E8 label: pHA-UBC: 2293->2520"
                     /modified_by="u0063854"
                     /label="UBC_K7R"
     conflict        734..736
                     /created_by="u0063854"
                     /modified_by="u0063854"
                     /label="K1R"
     conflict        749..751
                     /created_by="u0063854"
                     /modified_by="u0063854"
                     /label="K2R"
     conflict        797..799
                     /created_by="u0063854"
                     /modified_by="u0063854"
                     /label="K3R"
     conflict        803..805
                     /created_by="u0063854"
                     /modified_by="u0063854"
                     /label="K4R"
     conflict        815..817
                     /created_by="u0063854"
                     /modified_by="u0063854"
                     /label="K5R"
     conflict        860..862
                     /created_by="u0063854"
                     /modified_by="u0063854"
                     /label="K6R"
     conflict        905..907
                     /created_by="u0063854"
                     /modified_by="u0063854"
                     /label="K7R"
     domain          970..1554
                     /created_by="u0063854"
                     /label="IRES"
     CDS             1587..1985
                     /created_by="User"
                     /modified_by="u0063854"
                     /label="Blasticidin"
     domain          2003..2578
                     /Source="Geneious"
                     /label="WPRE"
     domain          2644..2659
                     /Source="Geneious"
                     /label="cPPT"
     domain          2661..2713
                     /Source="Geneious"
                     /label="delta_U3"
     domain          2714..2894
                     /Source="Geneious"
                     /label="HIV-1_5_LTR"
     domain          2975..3094
                     /Source="Geneious"
                     /label="SV40_PA_terminator"
     domain          3112..3210
                     /Source="Geneious"
                     /label="SV40_origin"
     domain          3326..3474
                     /Source="Geneious"
                     /label="lacZ_a"
     domain          3492..3798
                     /Source="Geneious"
                     /label="f1_origin"
     domain          4061..4921
                     /Source="Geneious"
                     /label="Ampicillin"
     domain          5076..5695
                     /Source="Geneious"
                     /label="pBR322_origin"
     domain          6376..6548
                     /Source="Geneious"
                     /label="HIV-1_5_LTR"
     domain          6659..6702
                     /Source="Geneious"
                     /label="HIV-1_psi_pack"
     domain          7209..7442
                     /Source="Geneious"
                     /label="RRE"
     domain          7858..7975
                     /created_by="User"
                     /modified_by="User"
                     /label="central DNA flap"
     domain          7862..7877
                     /Source="Geneious"
                     /modified_by="User"
                     /label="cPPT"
     domain          7960..7971
                     /created_by="User"
                     /label="CTS"
ORIGIN      
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacgggccc tctagaccat ggaacagaag ctgatctccg aggaggacct
      721 tcagatcttc gtgaggaccc tgactggtag gaccatcact ctcgaagtgg agccgagtga
      781 caccattgag aatgtcaggg caaggatcca agacagggaa ggcatccctc ctgaccagca
      841 gaggttgatc tttgctggga gacagctgga agatggacgc accctgtctg actacaacat
      901 ccagagagag tccaccctgc acctggtact ccgtctcaga ggtgggtgag gtaccggatc
      961 cggaattccg cccccccccc ccccctctcc ctcccccccc ctaacgttac tggccgaagc
     1021 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     1081 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     1141 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     1201 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     1261 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     1321 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     1381 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     1441 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     1501 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc
     1561 cacaacccac aaggagacga ccttccatgg ccaagccttt gtctcaagaa gaatccaccc
     1621 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     1681 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     1741 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     1801 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     1861 ggtgccgaca ggtgcttctc gatctgcacc ctgggatcaa agccatagtg aaggacagtg
     1921 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     1981 gctgaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     2041 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2101 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2161 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg cggcgtggtg tgcactgtgt
     2221 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2281 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2341 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     2401 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     2461 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     2521 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     2581 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtaaatc ttagccactt
     2641 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     2701 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2881 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     2941 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     3001 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3061 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     3121 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3181 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3241 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     3301 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     3361 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     3421 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     3481 cctgtagcgg cgcattaagc gcggcgggtg aggtggttac gcgcagcgtg accgctacac
     3541 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3601 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3661 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3721 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3781 tgttccgaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3841 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3901 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     3961 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4021 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4081 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4141 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4201 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4261 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4321 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4381 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4441 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4501 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4561 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4621 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4681 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4741 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4801 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4861 tatggatgaa cgaaatagac agatcgctga gataggcgcc tcactgatta agcattggta
     4921 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4981 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5221 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5701 tttacggttc ctggtctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5761 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5821 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5881 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5941 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     6001 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     6061 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     6121 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     6181 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     6241 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     6301 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     6361 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6421 tactcgggaa cccgctgctt taagcctgca ataaagctgc cctgagattt gtccgtagta
     6481 taccagtctt gtgactctgg taactagaga tccctagacg ctttagtcag tgtggaaaat
     6541 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     6601 cgacgcagga ctcggcttcc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     6661 agtacgccaa aaattttgac tagcgaaagc tcaaggagag agatgggtgc gagagcgtcg
     6721 tctgggcgga gagtaaatag cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     6781 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacaatcg cagttaatcc
     6841 tggcctgttc gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     6901 tcggacagga tcagaagaac ttagatcatt atataatacg ataacagaga ccctctattg
     6961 tgtgcatcaa aggataaaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     7021 gcaaaacaaa agtaagacca ccgcacacag cagagcggcc gctgatcttc agacctggag
     7081 gaggaatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     7141 accattagga tgagcaccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     7201 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     7261 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     7321 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     7381 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     7441 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     7501 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     7561 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     7621 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     7681 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     7741 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     7801 tcaccattat cgtttcagac ccacctccca accccgaggg gacccagaga gggcctattt
     7861 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     7921 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     7981 ttattacagg gacagcagag atccactttg gcgccggctc gag
//
