LOCUS       pLV-puro-EF1A-3XFLAG-hASB8_ANK3-4        9431 bp    DNA     circular SYN 18-AUG-2025
DEFINITION  .
ACCESSION   VB200306-1537ajt
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Promoter        1..229
                     /application_notes="Strong promoter; drives transcription
                     of viral RNA in packaging cells."
                     /description="Rous sarcoma virus enhancer/promoter"
                     /full_name="Rous sarcoma virus (RSV) enhancer/promoter"
                     /note="color: #ef6cdf; direction: RIGHT"
                     /official_designation="RSV"
                     /uuid="3f2ddca7f0cb4015b6abcec8a25c834e"
                     /label="RSV  promoter"
     LTR             230..411
                     /application_notes="Allows transcription of viral RNA and
                     its packaging into virus."
                     /description="Truncated HIV-1 5' long terminal repeat"
                     /full_name="HIV-1 truncated 5' LTR"
                     /note="color: #c54b7c; direction: RIGHT"
                     /official_designation="Delta5' LTR"
                     /uuid="44156a3083604321be44066133e8ecb9"
                     /label="5' LTR-ΔU3"
     motif           1076..1309
                     /application_notes="Rev protein binding site that allows
                     Rev-dependent nuclear export of viral RNA during viral
                     packaging."
                     /description="HIV-1 Rev response element"
                     /full_name="HIV-1 Rev response element"
                     /note="color: #fd3434; direction: RIGHT"
                     /official_designation="RRE"
                     /uuid="df07099e49674cf3aba3661b54e28bd7"
                     /modified_by="u0063854"
                     /label="RRE"
     motif           1804..1921
                     /application_notes="Facilitates the nuclear import of
                     HIV-1 cDNA through a central DNA flap."
                     /description="Central polypurine tract"
                     /full_name="Central polypurine tract"
                     /note="color: #46c6ef; direction: RIGHT"
                     /official_designation="cPPT"
                     /uuid="f7cb29985f9f41a7a5924d3462266f42"
                     /modified_by="u0063854"
                     /label="cPPT"
     Promoter        1960..3138
                     /application_notes="Strong promoter."
                     /description="Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /full_name="Human eukaryotic translation elongation
                     factor1 alpha1 promoter"
                     /note="color: #eda481; direction: RIGHT"
                     /official_designation="EF1A"
                     /uuid="69c052aac71a4f49bab5b7ae1f46ace8"
                     /label="EF1A"
     CDS             3169..3171
                     /created_by="u0063854"
                     /label
     motif           3172..3237
                     /created_by="User"
                     /modified_by="u0063854"
                     /label="3xFLAG"
     CDS             3238..4104
                     /codon_start=1
                     /gene="ASB8-fl(1-288)"
                     /translation="MSSSMWYIMQSIQSKYSLSERLIRTIAAIRSFPHDNVEDLIRGG
                     ADVNCTHGTLKPLHCACMVSDADCVELLLEKGAEVNALDGYNATALHYAAAADEACVE
                     VLLEYGANPNALDGNRDTPLHGAAFGNNAECVRALLESGASVNALDYNNDTPLSWAAM
                     KGNLESVSILLDYGAEVRVINLIGQTPISRLVALLVRGLGTEKEDSCFELLHRAVGHF
                     ELRKNGTMPREVARDPQLCEKLTVLCSAPGTLKTLARYAVRRSLGLQYLPDAVKGLPL
                     PASLKEYLLLLE"
                     /modified_by="u0063854"
                     /label="ASB8: ANK3-4"
     conflict        3496..3498
                     /created_by="u0063854"
                     /label="R87A"
     conflict        3520..3522
                     /created_by="u0063854"
                     /modified_by="u0063854"
                     /label="E95A"
     conflict        3523..3525
                     /created_by="u0063854"
                     /label="K96A"
     conflict        3607..3609
                     /created_by="u0063854"
                     /label="W124G"
     conflict        3619..3621
                     /created_by="u0063854"
                     /label="K128G"
     motif           4143..4740
                     /application_notes="Enhances virus stability in packaging
                     cells, leading to higher titer of packaged virus; enhances
                     higher expression of transgenes."
                     /description="Woodchuck hepatitis virus
                     posttranscriptional regulatory element"
                     /full_name="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #e4b930; direction: RIGHT"
                     /official_designation="WPRE"
                     /uuid="0aedff8308474b369c622433538d7836"
                     /modified_by="u0063854"
                     /label="WPRE"
     Promoter        4759..5268
                     /application_notes="Medium-strength promoter."
                     /description="Mouse phosphoglycerate kinase 1 promoter"
                     /full_name="Mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #7a00b5; direction: RIGHT"
                     /official_designation="mPGK promoter"
                     /uuid="bce93795b5fd4173b8b9abc832edad80"
                     /label="mPGK promoter"
     CDS             5281..5880
                     /application_notes="Allows cells to be resistant to
                     puromycin."
                     /description="Puromycin resistance gene"
                     /full_name="Puromycin resistance gene"
                     /note="color: #05696d; direction: RIGHT"
                     /official_designation="Puro"
                     /uuid="39d7413208494d9291d10b21b13ca44c"
                     /modified_by="u0063854"
                     /label="Puro"
     LTR             5951..6185
                     /application_notes="Allows packaging of viral RNA into
                     virus; self-inactivates the 5' LTR by a copying mechanism
                     during viral genome integration; contains polyadenylation
                     signal for transcription termination."
                     /description="Truncated HIV-1 3' long terminal repeat"
                     /full_name="HIV-1 truncated 3' LTR"
                     /note="color: #f54c6b; direction: RIGHT"
                     /official_designation="DeltaU3/3' LTR"
                     /uuid="c958755414c34372b909e20e81ebcef6"
                     /label="3' LTR-ΔU3"
     PolyA_signal    6258..6392
                     /application_notes="Allows transcription termination and
                     polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /description="Simian virus 40 early polyadenylation
                     signal"
                     /full_name="SV40 early polyadenation signal"
                     /note="color: #944603; direction: RIGHT"
                     /official_designation="SV40 early pA"
                     /uuid="2fcb13a62e704d8095b188d9625a6a94"
                     /label="SV40 early pA"
     motif           7346..8206
                     /application_notes="Allows E. coli to be resistant to
                     ampiciIIin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /note="color: #ce0084; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="6a7994dc58024d80b36e4a85ad2e1d99"
                     /modified_by="u0063854"
                     /label="AmpiciIIin"
     Rep_origin      8377..8965
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /note="color: #6d3ed8; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="64a8cb3def9f49deb6752795c2d893a8"
                     /label="pUC ori"
ORIGIN      
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactgagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
      421 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagattat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgct
     1801 agcttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tactagtgat tatcggatca actttgtata gaaaagttgg gctccggtgc ccgtcagtgg
     1981 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     2041 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     2101 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     2161 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     2221 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     2281 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     2341 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     2401 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     2461 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     2521 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     2581 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     2641 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gtctcgcgcc gccgtgtatc
     2701 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     2761 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     2821 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     2881 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     2941 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3001 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     3061 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     3121 ccatttcagg tgtcgtgaca agtttgtaca aaaaagcagg ctgccaccat ggactacaaa
     3181 gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga tgacaagatg
     3241 agttccagta tgtggtatat tatgcagagc attcagagca aatactctct ctccgagcgc
     3301 ttaatccgaa caattgctgc catccgttcc ttcccacatg ataatgtaga ggacctcatc
     3361 agagggggag cagatgtgaa ctgcactcat ggcacactga agcccttgca ctgtgcctgt
     3421 atggtgtcag atgctgactg tgtggagtta cttctggaaa aaggagccga ggtgaatgcc
     3481 ctggatgggt ataacgcaac agccctccac tatgcagcag cggcagatga ggcttgtgtg
     3541 gaggtcctat tggagtatgg tgcaaacccc aatgctttgg atggcaacag agatacccca
     3601 cttcacgggg cagcctttgg gaacaatgct gagtgtgtgc gggctctcct agagagcggg
     3661 gcctctgtca atgccctgga ttacaacaat gatacaccgc tcagctgggc tgccatgaag
     3721 ggaaatcttg agagtgtcag catccttctg gattatggcg cagaggtcag agtcatcaac
     3781 ctaataggcc agacacccat ctcccgcctg gtggctctgc tagtcagggg acttggaaca
     3841 gagaaagagg actcttgctt tgagctcctc cacagagctg ttggacactt tgaattgagg
     3901 aaaaatggca ccatgccacg agaggtggcc agagacccgc agctatgtga aaaactgact
     3961 gttctgtgct cagctccagg aactctaaaa acactcgctc gctatgccgt gcgccgtagc
     4021 ctgggactcc agtatctccc cgatgcagtg aagggccttc cactgccagc ttctttgaag
     4081 gaatacctgt tacttttaga ataaacccag ctttcttgta caaagtggtg ataatcgaat
     4141 tccgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4201 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4261 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4321 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4381 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4441 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4501 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     4561 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4621 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4681 cgtcttcgcc ttcgccctca gacgagtcgg atttcccttt gggccgcctc cccgcatcgg
     4741 gaattcccgc ggttcgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     4801 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     4861 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     4921 ccaccttcta ctcctcccct agtcaggaag ttccccccgc cccgcagctc gcgtcgtgca
     4981 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     5041 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     5101 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     5161 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     5221 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctca cgtggccacc
     5281 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     5341 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5401 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5461 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     5521 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5581 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5641 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     5701 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5761 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5821 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     5881 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5941 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     6001 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6061 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6121 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6181 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     6241 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6301 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6361 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     6421 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6481 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6541 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     6601 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6661 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6721 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     6781 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6841 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6901 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6961 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     7021 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7081 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7141 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     7201 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7261 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7321 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7381 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7441 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7501 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7561 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7621 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7681 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7741 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7801 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7861 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7921 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7981 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8041 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8101 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8161 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8221 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8281 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8341 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8401 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8461 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8521 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8581 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8641 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8701 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8761 gcgtgagcta tgagaaagcg ccacgcttcc cgaagagaga aaggcggaca ggtatccggt
     8821 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8881 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8941 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9001 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9061 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9121 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     9181 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9241 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9301 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9361 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     9421 gctgcaagct t
//
