LOCUS       pAAV-CAG-tdT-P2A-Mxra8  7563 bp ds-DNA     circular     09-JUL-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2..131
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1103..1152
                     /label="PHK_41"
                     /note="sequence: gtctcatcattttggcaaagaattgctagccaccatggtgagtaagggcg"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1106..1150
                     /label="PHK_36"
                     /note="sequence: tcatcattttggcaaagaattgctagccaccatggtgagcaaggg"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1125..1156
                     /label="PrimerHK15_pcDNA3.1+mCherry_F"
                     /note="sequence: GGAGACCCAAGCTGGCTAGCcaccatggtgagcaagggcgagga"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1131..1154
                     /label="PHK_28"
                     /note="sequence: gatccgccaccatggtgagcaagggcgag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1131..1154
                     /label="PHK_29"
                     /note="sequence: gtgaggatccgccaccatggtgagcaagggcgag"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1131..1156
                     /label="PrimerHK11_pAAV+mCherry_F"
                     /note="sequence: ccgcggccgccaccatggtgagcaagggcgagga"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1135..1156
                     /label="PrimerHK25"
                     /note="sequence: CCACTAACTTCTCCCTGTTGAAACAAGCAGGAGATGTCGAAGAGAATCCTGGGCCAatggtgagcaagggcgagga"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1135..1160
                     /label="PHK_58"
                     /note="sequence: gctcggtacccggggatccatggtgagtaagggcgaggaagtg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1137..2564
                     /label="tdTomato"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1137..2564
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFLYGMDELYK"
     primer          complement(1605..1623)
                     /label="PrimerHK20"
                     /note="sequence: aggactcctccctgcaggac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1703..1721
                     /label="MAPseqPCR1F"
                     /note="sequence: tgctgcccgacaaccactac"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2331..2349)
                     /label="PrimerHK20"
                     /note="sequence: aggactcctccctgcaggac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2535..2587
                     /label="PrimerHK17"
                     /note="sequence: tccaccggcggcatggacgagctgtacaagGGAAGCGGAGCCACTAACTTCTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2536..2610)
                     /label="PHK_42"
                     /note="sequence: CATCTCCTGCTTGTTTCAACAGGGAGAAGTTAGTGGCTCCGCTTCCcttatacagctcatccatgccgtacagaa"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          2543..2560
                     /label="MAPseqPCR1F_nested original"
                     /note="sequence: ctcggcatggacgagctgta"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2543..2560
                     /label="Longwen Huang"
                     /note="sequence: ctcggcatggacgagctgta"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2543..2564)
                     /label="PrimerHK26"
                     /note="sequence: cgatatgatcctctggagatatcttacttgtacagctcgtccatgccg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2543..2587
                     /label="PrimerHK17"
                     /note="sequence: tccaccggcggcatggacgagctgtacaagGGAAGCGGAGCCACTAACTTCTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2543..2607)
                     /label="PrimerHK12_mCherry+P2A_R"
                     /note="sequence: CTCCTGCTTGTTTCAACAGGGAGAAGTTAGTGGCTCCGCTTCCcttgtacagctcgtccatgccg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2565..2630
                     /label="P2A"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             2565..2630
                     /label="Translation 2565-2630"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     primer          2589..2648
                     /label="PrimerHK13_P2A+mMxra8_F"
                     /note="sequence: CTGTTGAAACAAGCAGGAGATGTCGAAGAGAATCCTGGGCCAatggctcgctcggtgacc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2629..2647
                     /label="Primer HK7 (NcoI)mMXRA8_F"
                     /note="sequence: ccgcggccgccaccatggctcgctcggtgac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2629..2649
                     /label="PrimerHK22"
                     /note="sequence: agcgcagtcgccaccatggctcgctcggtgaccc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2629..2689
                     /label="PHK_33"
                     /note="sequence: catttcaggtgtcgtgagctagccatggctcgctcggtgaccctagtctttctggtgcttgtctcactgaccggcttgtatgc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          2631..2648
                     /label="PHK_50"
                     /note="sequence: tgtgaccggcggctctagatggctcgctcggtgacc"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2631..2732
                     /label="Signal peptide flag linker"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Sequence: ATGGCTCGCTCGGTGACCCTAGTCTTTCTGGTGCTTGTCTCACTGACCGGCTTGTATGCTATGGACTACAAGGACGACGATGACAAGCTCGATGGAGGATCC created_by: oferzimmerman"
     misc_feature    2631..3992
                     /label="mMXRA8"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="created_by: oferzimmerman"
     primer          2671..2730
                     /label="PHK_31"
                     /note="sequence: cactgaccggcttgtatgctatggactacaaggacgacgatgacaagctcgatggaggat"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          2711..2734
                     /label="PHK_37"
                     /note="sequence: tgacaagctcgatggaggatccac"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2727..2747)
                     /label="PrimerHK19"
                     /note="sequence: cccggaaggacctgaggatcc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3533..3552)
                     /label="PrimerHK13_P2A+mMxra8_F"
                     /note="sequence: CTGTTGAAACAAGCAGGAGATGTCGAAGAGAATCCTGGGCCAatggctcgctcggtgacc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(3829..3846)
                     /label="Nested2ndR (or Nested1stR, name changed since it was confusing)"
                     /note="sequence: CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(3962..3992)
                     /label="PrimerHK24"
                     /note="sequence: AACAGGGAGAAGTTAGTGGCTCCGCTTCCcttgcagtattcttttcgaaactccttgtcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3963..3995)
                     /label="PrimerHK16_pcDNA3.1+mMxra8_R"
                     /note="sequence: CAGTGGCGGCCGCTCGAGttacttgcagtattcttttcgaaactccttgtc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3963..3995)
                     /label="PrimerHK14_pAAV+mMxra8_R"
                     /note="sequence: ggtttaaacgggccctctagattacttgcagtattcttttcgaaactccttgtc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3963..4021)
                     /label="PHK_60"
                     /note="sequence: TCCAGAGGTTGATTATCGATAAGCTTttacttgcagtattcttttcgaaactccttgtc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3963..4022)
                     /label="PHK_35"
                     /note="sequence: atccagaggttgattatcgataagcttttacttgcagtattcttttcgaaactccttgtc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(3988..4021)
                     /label="PHK_61"
                     /note="sequence: TCCAGAGGTTGATTATCGATAAGCTTttacttgc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             3993..3995
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="*"
     primer          4005..4048
                     /label="PHK_54"
                     /note="sequence: acgaagttatcgaagatcatcaagcttctcgagataatcaacctctggattacaaaatttgtgaaagattgactgg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4008..4596
                     /label="WPRE"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          4025..4048
                     /label="SPD23 (WPRE forward)"
                     /note="sequence: caaaatttgtgaaagattgactgg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4120..4139)
                     /label="SPD24 (WPRE R)"
                     /note="sequence: aatgaaagccatacgggaag"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(4596..4615)
                     /label="PrimerHK21"
                     /note="sequence: gccacccgtagatctctcgagccgggtcgacggtatcgatg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4639..4760
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(4922..5051)
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(5270..5952)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(6050..6709)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      complement(7170..7476)
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(7495..7563)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
ORIGIN
        1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
      181 taggaagagt accattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      241 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      301 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      361 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      421 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      481 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      541 tgggggcggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      601 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      661 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      721 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggc tgtccgcggg
      961 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1021 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1081 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgcta gccaccatgg
     1141 tgagtaaggg cgaggaagtg atcaaagagt tcatgcggtt taaggtgaga atggaaggaa
     1201 gcatgaacgg ccacgagttc gaaattgagg gagaaggaga gggacggccc tacgagggca
     1261 cccagacagc caagctgaaa gtgacaaagg gcgggcctct gccattcgct tgggacatcc
     1321 tgagcccaca gtttatgtac ggctccaagg cctatgtgaa acatccagct gacattcccg
     1381 attataagaa actgagcttc cccgaggggt ttaagtggga aagagtgatg aacttcgagg
     1441 acggaggcct ggtgactgtg acccaggaca gctccctgca ggatgggacc ctgatctaca
     1501 aggtgaaaat gagagggaca aattttcccc ctgatggacc tgtgatgcag aagaaaacta
     1561 tgggatggga ggcctccacc gaaaggctgt atccacgcga cggggtgctg aaaggagaaa
     1621 tccaccaggc tctgaagctg aaagatgggg gacattacct ggtggagttc aagacaatct
     1681 acatggccaa gaaacctgtg cagctgccag gctactatta cgtggacaca aaactggata
     1741 tcacttcaca caacgaggac tacactattg tggagcagta tgaacggagc gaggggagac
     1801 accatctgtt cctgggccat gggactggaa gtaccggctc agggtctagt ggaaccgcct
     1861 caagcgagga taacaatatg gctgtgatca aagagttcat gaggtttaag gtgcgcatgg
     1921 agggcagcat gaatgggcac gaatttgaga ttgaaggaga gggcgaaggg aggccttacg
     1981 agggcacaca gactgccaag ctgaaagtga ccaagggagg accactgcct ttcgcttggg
     2041 atatcctgtc tcctcagttt atgtacggaa gtaaggccta tgtcaagcat cccgctgaca
     2101 ttcctgatta caagaaactg tctttcccag agggctttaa gtgggagaga gtgatgaatt
     2161 ttgaagatgg aggcctggtg accgtgacac aggactcctc tctgcaggat ggcactctga
     2221 tctacaaagt caaaatgcgc ggcaccaatt ttccacccga tgggcccgtg atgcagaaga
     2281 aaacaatggg gtgggaggcc agcactgaac ggctgtatcc tagagacgga gtgctgaagg
     2341 gcgaaatcca ccaggccctg aagctgaaag acggcggcca ctacctggtg gagttcaaaa
     2401 ccatctacat ggccaagaaa ccagtgcagc tgcccggcta ttactatgtg gacaccaagc
     2461 tggatatcac atcccacaat gaagactaca ccattgtgga acagtatgag aggtctgaag
     2521 gacgccacca tctgtttctg tacggcatgg atgagctgta taagGGAAGC GGAGCCACTA
     2581 ACTTCTCCCT GTTGAAACAA GCAGGAGATG TCGAAGAGAA TCCTGGGCCA atggctcgct
     2641 cggtgaccct agtctttctg gtgcttgtct cactgaccgg cttgtatgct atggactaca
     2701 aggacgacga tgacaagctc gatggaggat cctcaggtcc ttccgggaca gcagcagcat
     2761 cctcttcatt ggtctccgaa agtgtggttt ctttggcagc cggtacacaa gcagtccttc
     2821 gctgtcaatc cccccgaatg gtttggaccc aagacaggtt gcacgaccgc caaagggtag
     2881 tccattggga tttgtcaggt ggtcctggga gccaaaggcg aaggcttgtt gatatgtaca
     2941 gtgcagggga acaacgggtg tacgaaccac gagaccggga tcggctgctc ctcagtccct
     3001 ccgcattcca tgacggaaat ttctctctct tgataagggc agtggataga ggagatgagg
     3061 gggtttatac atgtaacctg catcatcact actgccacct tgacgaatct cttgcagtcc
     3121 gccttgaagt taccgaagat cccctcctta gccgagctta ttgggatggc gaaaaggagg
     3181 tcttggtagt cgcacacggt gctccagccc tgatgacatg tatcaatcga gcccacgttt
     3241 ggacagatcg ccatttggaa gaggcccaac aagttgtaca ttgggacaga cagttgccag
     3301 gcgtctcaca cgatcgagcc gataggcttt tggatctcta cgcaagtggc gagcggaggg
     3361 cttacggacc tcccttcctc cgagacaggg taagtgtcaa tacaaatgct tttgcacgag
     3421 gcgacttcag ccttagaatc gatgaactcg aacgagcaga cgaggggatc tatagttgcc
     3481 atctccacca ccactactgc ggtcttcatg agcgcagagt cttccacctc caggtcacag
     3541 agccagcctt tgagccacca gccagagcct caccaggtaa tggatcaggg cacagctcag
     3601 cacccagtcc agaccctact ctcaccagag gacattctat cataaatgtc attgttcctg
     3661 aggaccatac tcacttcttt cagcagctcg ggtacgtgct cgctactctg ctgcttttca
     3721 tcctgcttct gataactgtt gttcttgcta caagatatcg acactctggg ggttgtaaga
     3781 cctccgacaa aaaggccggg aagagtaagg gtaaggatgt caacatggtc gaattcgccg
     3841 ttgccactcg cgatcaagct ccttatagaa ccgaggacat acaacttgat tacaaaaata
     3901 acattcttaa agagagggct gagttggctc actcaccatt gcccgccaaa gatgtggatt
     3961 tggacaagga gtttcgaaaa gaatactgca agtaaaagct tatcgataat caacctctgg
     4021 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4081 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4141 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4201 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4261 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4321 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4381 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     4441 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4501 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4561 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cccgggcggc
     4621 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     4681 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     4741 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     4801 gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
     4861 aggatcttcc tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac
     4921 aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4981 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     5041 cgagcgcgca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5101 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5161 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5221 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5281 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5341 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5401 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5461 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5521 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5581 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5641 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5701 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5761 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5821 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5881 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5941 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6001 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6061 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6121 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6181 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6241 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6301 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6361 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6421 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6481 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6541 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6601 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6661 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6721 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6781 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6841 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6901 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6961 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7021 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     7081 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     7141 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     7201 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     7261 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     7321 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     7381 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     7441 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     7501 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     7561 cca
//