LOCUS       DH-BB[5'SIN;181/25ORF]  6702 bp ds-DNA     circular     09-JUL-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          complement(667..699)
                     /label="Primer HK4CHIKV-Ins1+2_R"
                     /note="sequence: CCCCACGTGACCTCtAGCCCTTCAGTCGGCACG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          685..705
                     /label="Primer HK5 CHIKV-Ins1+2-F"
                     /note="sequence: aGAGGTCACGTGGGGCAACAA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    977..1159
                     /label="6K"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1160..2476
                     /label="E1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2457..2479)
                     /label="Primer HK6 CHIKV-Ins2_R"
                     /note="sequence: TTAGTGCCTGCTAAACGACACGC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          2462..2499
                     /label="PrimerHK1_JK114+Ins2_F"
                     /note="sequence: TCGTTTAGCAGGCACTAACCGCTACGCCCCAATGATCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2462..2499
                     /label="Ins2+JK114_vector_F"
                     /note="sequence: TCGTTTAGCAGGCACTAACCGCTACGCCCCAATGATCC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2492..2517
                     /label="conserved to sinRep5"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             3200..3859
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4722..4723
                     /label="5'end of sindbis RNA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    5147..5156
                     /label="BB-scar"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5390..5448
                     /label="subgenomic promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(5434..5482)
                     /label="Primer HK2 JK114_vector+Ins1_R"
                     /note="sequence: GGGTTGGGATAAACTCCATGGTGGTGGTGTTGTAGTATTAGTCAGATGA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             5464..685
                     /label="CHIKVgp2"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="codon_start: 1 transl_table: 1 translation: MEFIPTQTFYNRRYQPRPWTPRPTIQVIRPRPRPQRKAGQLAQLISAVNKLTMRAVPQQKPRKNRKNKKQKQKQQAPRNNMKQKKQPPKKKPAQKKKKPGRRERMCMKIENDCIFEVKHEGKVTGYACLVGDKVMKPAHVKGTIDNADLAKLAFKRSSKYDLECAQIPVHMKSDASKFTHEKPEGYYNWHHGAVQYSGGRFTIPTGAGKPGDSGRPIFDNKGRVVAIVLGGANEGARTALSVVTWNKDIVTKITPEGAEEWSLAIPVMCLLANTTFPCSQPPCTPCCYEKEPEKTLRMLEDNVMSPGYYQLLQASLTCSPRRQRRSIKDNFNVYKAIRPYLAHCPDCGEGHSCHSPVALERIRNEATDGTLKIQVSLQIGIKTDDSHDWTKLRYMDNHMPADAERARLFVRTSAPCTITGTMGHFILARCPKGETLTVGFTDGRKISHSCTHPFHHDPPVIGREKFHSRPQHGRELPCSTYAQSTAATAEEIEVHMPPDTPDRTLMSQQSGNVKITVNSQTVRYKCNCGDSNEGLTTTDKVINNCKVDQCHAAVTNHKKWQYNSPLVPRNAELGDRKGKVHIPFPLANVTCRVPKARNPTVTYGKNQVIMLLYPDHPTLLSYRNMGEEPNYQEEWVTHKKEIRLTVPTEGLEVTWGNNEPYKYWPQLSTNGTAHGHPHEIILYYYELYPTMTVVVVSVASFVLLSMVGVAVGMCMCARRRCITPYELTPGATVPFLLSLICCIRTAKAATYQEAAVYLWNEQQPLFWLQALIPLAALIVLCNCLRLLPCFCKTLTFLAVMSVGAHTVSAYEHVTVIPNTVGVPYKTLVNRPGYSPMVLEMELLSVTLEPTLSLDYITCEYKTVIPSPYVKCCGTAECKDKSLPDYSCKVFTGVYPFMWGGAYCFCDTENTQLSEAHVEKSESCKTEFASAYRAHTASASAKLRVLYQGNNVTVSAYANGDHAVTVKDAKFIVGPMSSAWTPFDNKIVVYKGDVYNMDYPPFGAGRPGQFGDIQSRTPESEDVYANTQLVLQRPSAGTVHVPYSQAPSGFKYWLKERGASLQHTAPFGCQIATNPVRAMNCAVGNMPISIDIPDAAFTRVVDAPSLTDMSCEVPACTHSSDFGGVAIIKYAASKKGKCAVHSMTNAVTIREAEIEVEGNSQLQISFSTALASAEFRVQVCSTQVHCAAECHPPKDHIVNYPASHTTLGVQDISVTAMSWVQKITGGVGLVVAVAALILIVVLCVSFSRH*"
     misc_feature    5464..2479
                     /label="CHIKV-structural protein ORF"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          5464..5493
                     /label="Primer HK3 CHIKV-Ins1_F"
                     /note="sequence: ATGGAGTTTATCCCAACCCAAACTTTCTAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    5464..6217
                     /label="Capsid"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(5686..5708)
                     /label="PrimerHK10_CHIKV_correction_R"
                     /note="sequence: TTCATGTTGTTTCGTGGCGCCTG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     5986..6005
                     /label="8,118 F"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="%GC: 60.0 Hairpin Tm: None Self Dimer Tm: None Tm: 60.0 Pair Dimer Tm: 4.1 annotation group: 68174235576647006f3c97d3-d31d-49d7-ab11-68799873d404 Sequence: GGGGTACTACAACTGGCACC created by: primer3 Product Size: 192 # Off-target Sites: 0 Off-target Template: CHIKV 181 25 Plasmid (1)"
     protein_bind    6110..6133
                     /label="hnRNPK Binding Site"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="bound_moiety: hnRNPK recgonition sequence"
     misc_feature    6218..6409
                     /label="E3"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    6410..976
                     /label="E2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 ACACTTCATC CTGGCCCGAT GTCCGAAAGG AGAAACTCTG ACGGTGGGAT TCACTGACGG
       61 TAGGAAGATC AGTCACTCAT GTACGCACCC ATTTCACCAC GACCCTCCTG TGATAGGCCG
      121 GGAAAAATTT CATTCCCGAC CGCAGCACGG TAGAGAACTA CCTTGCAGCA CGTACGCGCA
      181 GAGCACCGCT GCAACTGCCG AGGAGATAGA GGTACATATG CCCCCAGACA CCCCAGATCG
      241 CACATTGATG TCACAACAGT CCGGTAATGT AAAGATCACA GTCAATAGTC AGACGGTGCG
      301 GTACAAGTGT AATTGCGGTG ACTCAAATGA AGGACTAACC ACTACAGACA AAGTGATTAA
      361 TAACTGCAAG GTTGATCAAT GCCATGCCGC GGTCACCAAT CACAAAAAAT GGCAGTATAA
      421 TTCCCCTCTG GTCCCGCGTA ATGCTGAACT CGGGGACCGA AAAGGAAAAG TTCACATTCC
      481 GTTTCCTCTG GCAAATGTGA CATGCAGGGT GCCTAAGGCA AGGAACCCCA CCGTGACGTA
      541 CGGAAAAAAC CAAGTCATCA TGCTGCTGTA TCCTGACCAC CCAACGCTCC TGTCCTACCG
      601 GAATATGGGA GAAGAACCAA ACTATCAAGA AGAGTGGGTG ACGCATAAGA AGGAGATCAG
      661 GTTAACCGTG CCGACTGAAG GGCTaGAGGT CACGTGGGGC AACAACGAGC CGTACAAGTA
      721 TTGGCCGCAG TTATCCACAA ACGGTACAGC CCACGGCCAC CCGCATGAGA TAATTTTGTA
      781 TTATTATGAG CTGTACCCTA CTATGACTGT GGTAGTTGTG TCAGTGGCCT CGTTCGTACT
      841 CCTGTCGATG GTGGGTGTGG CAGTGGGGAT GTGCATGTGT GCACGACGCA GATGCATTAC
      901 ACCGTACGAA CTGACACCAG GAGCTACCGT CCCTTTCCTG CTTAGCCTAA TATGCTGCAT
      961 TAGAACAGCT AAAGCGGCCA CATACCAAGA GGCTGCGGTA TACCTGTGGA ACGAGCAGCA
     1021 GCCTTTGTTT TGGCTGCAAG CCCTTATTCC GCTGGCAGCC CTGATTGTCC TATGCAACTG
     1081 TCTGAGACTC TTACCATGCT TTTGTAAAAC GTTGACTTTT TTAGCCGTAA TGAGCGTCGG
     1141 TGCCCACACT GTGAGCGCGT ACGAACACGT AACAGTGATC CCGAACACGG TGGGAGTACC
     1201 GTATAAGACT CTAGTCAACA GACCGGGCTA CAGCCCCATG GTACTGGAGA TGGAGCTTCT
     1261 GTCAGTCACT TTGGAGCCAA CGCTATCGCT TGATTACATC ACGTGCGAGT ATAAAACCGT
     1321 CATCCCGTCT CCGTACGTGA AATGCTGCGG TACAGCAGAG TGCAAGGACA AGAGCCTACC
     1381 TGATTACAGC TGTAAGGTCT TCACCGGCGT CTACCCATTC ATGTGGGGCG GCGCCTACTG
     1441 CTTCTGCGAC ACTGAAAATA CGCAATTGAG CGAAGCACAT GTGGAGAAGT CCGAATCATG
     1501 CAAAACAGAA TTTGCATCAG CATATAGGGC TCATACCGCA TCCGCATCAG CTAAGCTCCG
     1561 CGTCCTTTAC CAAGGAAATA ATGTTACTGT ATCTGCTTAT GCAAACGGCG ATCATGCCGT
     1621 CACAGTTAAG GACGCTAAAT TCATTGTGGG GCCAATGTCT TCAGCCTGGA CACCTTTTGA
     1681 CAATAAAATC GTGGTGTACA AAGGCGACGT CTACAACATG GACTACCCGC CCTTCGGCGC
     1741 AGGAAGACCA GGACAATTTG GCGACATCCA AAGTCGCACG CCTGAGAGCG AAGACGTCTA
     1801 TGCTAACACA CAACTGGTAC TGCAGAGACC GTCCGCGGGT ACGGTGCACG TGCCGTACTC
     1861 TCAGGCACCA TCTGGCTTCA AGTATTGGCT AAAAGAACGA GGGGCGTCGC TGCAGCACAC
     1921 AGCACCATTT GGCTGTCAAA TAGCAACAAA CCCGGTAAGA GCGATGAACT GCGCCGTAGG
     1981 GAACATGCCT ATCTCCATCG ACATACCGGA CGCGGCCTTC ACTAGGGTCG TCGACGCGCC
     2041 ATCTTTAACG GACATGTCGT GTGAGGTACC AGCCTGCACC CACTCCTCAG ACTTTGGGGG
     2101 CGTAGCCATC ATTAAATATG CAGCCAGCAA GAAAGGCAAG TGTGCGGTGC ATTCGATGAC
     2161 TAACGCCGTC ACTATTCGGG AAGCTGAAAT AGAAGTAGAA GGGAACTCTC AGTTGCAAAT
     2221 CTCTTTTTCG ACGGCCCTAG CCAGCGCCGA ATTCCGCGTA CAAGTCTGTT CTACACAAGT
     2281 ACACTGTGCA GCCGAGTGCC ATCCACCGAA AGACCATATA GTCAATTACC CGGCGTCACA
     2341 CACCACCCTC GGGGTCCAAG ACATTTCCGT TACGGCGATG TCATGGGTGC AGAAGATCAC
     2401 GGGAGGTGTG GGACTGGTTG TCGCTGTTGC AGCACTGATC CTAATCGTGG TGCTATGCGT
     2461 GTCGTTTAGC AGGCACTAAC CGCTACGCCC CAATGATCCG ACCAGCAAAA CTCGATGTAC
     2521 TTCCGAGGAA CTGATGTGCA TAATGCATCA GGCTGGTACA TTAGATCCCC GCTTACCGCG
     2581 GGCAATATAG CAACACTAAA AACTCGATGT ACTTCCGAGG AAGCGCAGTg CATAATGCTG
     2641 CGCaGTGTTG CCACATAACC ACTATATTAA CCATTTATCT AGCGGACGCC AAAAACTCAA
     2701 TGTATTTCTG AGGAAGCGTG GTGCATAATG CCACGCAGCG TCTGCATAAC TTTTATTATT
     2761 TCTTTTATTA ATCAACAAAA TTTTGTTTTT AACATTTCAA AAAAAAAAAA AAAAAAAAAA
     2821 AAAAAAAgGG GAATTCctcG AGGGGAATTA ATTCTTGAAG ACGAAAGGGC CAGGTGGCAC
     2881 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT
     2941 GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG
     3001 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC
     3061 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC
     3121 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC
     3181 CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC
     3241 CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT
     3301 GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT
     3361 ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT
     3421 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT
     3481 TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT
     3541 GCCTGtAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC
     3601 TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG
     3661 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC
     3721 TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA
     3781 CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC
     3841 CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA
     3901 TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT
     3961 GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT
     4021 tAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA
     4081 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA
     4141 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT
     4201 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT
     4261 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA
     4321 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT
     4381 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCtaTGAG AAAGCGCCAC
     4441 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA
     4501 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG
     4561 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA
     4621 AAACGCCAGC AACGCGAGCT Cgtatggaca tattgtcgtt agaacgcggc tacaattaat
     4681 acataacctt atgtatcata cacatacgat ttaggtgaca ctatagATTG ACGGCGTAGT
     4741 ACACACTATT GAATCAAACA GCCGACCAAT TGCACTACCA TCACAATGGA GAAGCCAGTA
     4801 GTAAACGTAG ACGTAGACCC CCAGAGTCCG TTTGTCGTGC AACTGCAAAA AAGCTTCCCG
     4861 CAATTTGAGG TAGTAGCACA GCAGGTCACT CCAAATGACC ATGCTAATGC CAGAGCATTT
     4921 TCGCATCTGG CCAGTAAACT AATCGAGCTG GAGGTTCCTA CCACAGCGAC GATCTTGGAC
     4981 ATAGGCAGCG CACCGGCTCG TAGAATGTTT TCCGAGCACC AGTATCATTG TGTCTGCCCC
     5041 ATGCGTAGTC CAGAAGACCC GGACCGCATG ATGAAATATG CCAGTAAACT GGCGGAAAAA
     5101 GCGTGCAAGA TTACAAACAA GAACTTGCAT GAGAAGATTA AGGATCTCCG GGATCCCCTG
     5161 AAAAGGCTGT TTAAGTTGGG TAAACCGCTC CCAGCCGACG ACGAGCAAGA CGAAGACAGA
     5221 AGACGCGCTC TGCTAGATGA AACAAAGGCG TGGTTTAGAG TAGGTATAAC AGGCACTTTA
     5281 GCAGTGGCCG TGACGACCCG GTATGAGGTA GACAATATTA CACCTGTCCT ACTGGCATTG
     5341 AGAACTTTTG CCCAGAGCAA AAGAGCATTC CAAGCCATCA GAGGGGAAAT AAAGCATCTC
     5401 TACGGTGGTC CTAAATAGTC AGCATAGTAC ATTTCATCTG ACTAATACTA CAACACCACC
     5461 ACCATGGAGT TTATCCCAAC CCAAACTTTC TACAATAGGA GGTACCAGCC TCGACCTTGG
     5521 ACTCCGCGCC CTACTATCCA AGTTATCAGA CCCAGACCGC GTCCGCAAAG GAAAGCCGGG
     5581 CAACTTGCCC AGCTGATCTC AGCAGTTAAT AAACTGACAA TGCGCGCGGT ACCTCAACAG
     5641 AAGCCGCGCA AGAATCGGAA GAATAAGAAG CAAAAGCAAA AGCAGCAGGC GCCACGAAAC
     5701 AACATGAACG GCTCAAAAGA AAAAGAAGCC GGGCCGTAGA GAGAGAATGT GCATGAAAAT
     5761 CGAAAATGAT TGCATCTTCG AAGTCAAGCA TGAAGGTAAG GTAACAGGTT ACGCGTGCTT
     5821 GGTAGGGGAC AAAGTAATGA AGCCAGCACA CGTAAAGGGG ACCATCGATA ATGCGGACCT
     5881 GGCCAAATTG GCCTTCAAGC GGTCATCTAA GTACGACCTT GAATGCGCGC AGATACCCGT
     5941 GCACATGAAG TCCGACGCTT CGAAGTTCAC CCATGAGAAA CCGGAGGGGT ACTACAACTG
     6001 GCACCACGGA GCAGTACAGT ACTCAGGAGG CCGGTTCACC ATCCCTACAG GTGCGGGCAA
     6061 ACCAGGGGAC AGCGGTAGAC CGATCTTCGA CAACAAGGGG CGCGTGGTGG CCATAGTTTT
     6121 AGGAGGAGCT AATGAAGGAG CCCGTACAGC CCTCTCGGTG GTGACCTGGA ACAAAGACAT
     6181 CGTCACGAAA ATCACCCCTG AGGGGGCCGA AGAGTGGAGT CTTGCCATTC CAGTTATGTG
     6241 CCTGCTGGCA AATACCACGT TCCCCTGCTC CCAGCCCCCT TGCACACCCT GCTGCTACGA
     6301 AAAAGAGCCG GAGAAAACCC TGCGCATGCT AGAAGACAAC GTCATGAGCC CCGGGTACTA
     6361 TCAGCTGCTA CAAGCATCCT TAACATGTTC TCCCCGCCGC CAGCGACGCA GTATTAAGGA
     6421 CAACTTCAAT GTCTATAAAG CCATAAGACC GTACCTAGCT CACTGTCCCG ACTGTGGAGA
     6481 AGGGCACTCG TGCCATAGTC CCGTAGCGCT AGAACGCATC AGAAACGAAG CGACAGACGG
     6541 GACGCTGAAA ATCCAGGTTT CCTTGCAAAT CGGAATAAAG ACGGATGATA GCCATGATTG
     6601 GACCAAGCTG CGTTACATGG ACAATCATAT GCCAGCAGAC GCAGAGAGGG CCAGGCTATT
     6661 TGTAAGAACG TCAGCACCGT GCACGATTAC TGGAACAATG GG
//