LOCUS       Exported                8217 bp ds-DNA     circular SYN 08-SEP-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 9, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Kpn1-TPI-promoter-Xho1-EGFP-BamH1 in 416
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2010..3006
                     /label=-1000
     misc_feature    3750..4466
                     /label=EGFP coding
     misc_feature    complement(4473..4490)
                     /label=GAGA linker
     misc_feature    complement(4491..5210)
                     /label=EGFP
     primer_bind     complement(5441..5460)
                     /label=T3
     primer_bind     complement(5478..5498)
                     /label=M13-rev
     misc_binding    complement(5504..5526)
                     /label=LacO
     rep_origin      complement(5866..6548)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6646..7305)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtacca tgtccagtat ttactctacc ttgattcctt
     2041 ctcctgaact tcatttccta agccctagtt tcactccatt tacatcagat tatattcacg
     2101 atgatatagc gcataaaggc cattccagtt atgacgtcat gttagattta ttagaccctt
     2161 ccaattctct tgtctctacc gcgatgaaca atccaacata ttttaacgtt tataatacaa
     2221 tcattggtaa tgtagaacct cgtcagatat cgcgtgctat gaccaaatta caacaacgca
     2281 tcaaattccc ctcatggtcc tcttctgcga tgcacgttaa cattggaaga agatcgccat
     2341 acttgccttt acaaccaaat gaaaacgaag ttagcggcat gatgttaagc aatatgtcta
     2401 ccgtggtgaa cgtctttgag aatgcgtgca atacttttga caaagtattt gccaagggcg
     2461 catttttaaa taattataat gtaggcgact tgttccaatc aatgcagaac gttcaagatg
     2521 aattcgccga gtcaagagaa gtagtacaaa gcctgatgga ggattatgta gctgcagaac
     2581 aagattcgta cttggacgat gtacttgttg atgatgaaaa tatggttggc gagttggaag
     2641 aggacctgga tgccgacggt gatcataaat tagtataatg atgccttcct tgttcaggaa
     2701 ttttaccacc gcccaatagt tttattatag tactctcaat atatctatac caacaaccgc
     2761 ttcaaatgta ttatctccct ttataaattc tgcactgtat tgtaatatgt ataaagctct
     2821 gtaccacttt ttcatcattg catacatcga tagcgcctct tcaactttag aatttcctaa
     2881 agggtagaaa aaccagatga ttcaactcag cgtaatggaa cgctctgttt gaaataaaaa
     2941 acatctgtat cgctgcacgg attagttcga taattcaacg acagttatac taatcttggt
     3001 gatggaatga gtactttagc ggaagtaagt ttttgaatta tgcccccact ttttttttgt
     3061 tcatggtgac taacatgaat taggtttata ctattattga ggacgccgaa caggaatgtc
     3121 gaaaaggaga ttttacaaat gcaaaagcga agtatcaaga agccattgaa gttttaggtc
     3181 cgcagaacga gaatttgtct caaaataaat taagttctga cgttacgcaa gccattgacc
     3241 tattaaaaca ggacattaca gccaaaatcc aagaattgga acttttgatt gaaaagcagt
     3301 catcagaaga aaataatatt ggaatggtca acaacaatat gcttattggc tctgttattt
     3361 taaacaacaa atctccgata aatggaataa gcaatgctag gaattgggat aacccagcat
     3421 atcaggacac tttaagtccc ataaacgatc cacttcttat gtcaatttta aaccgcttac
     3481 aatttaattt gaataatgat attcaattga aaacagaagg aggaaaaaat tccaaaaact
     3541 cggaaatgaa gattaactta agactggaac aattcaaaaa ggagttggta ttatatgagc
     3601 agaaaaaatt taaagaatat ggtatgaaaa ttgacgaaat tacgaaagaa aataaaaaat
     3661 tggctaatga aattgggcga ttaagagaac gctgggacag cttagtagaa agtgcgaagc
     3721 aaagaaggga taagcaaaaa aacgtcgaga tggtgagcaa gggcgaggag ctgttcaccg
     3781 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3841 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3901 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3961 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     4021 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4081 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4141 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     4201 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     4261 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4321 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     4381 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4441 ctctcggcat ggacgagctg tacaagggat ccggagcagg ggcgggtgct atggtgagca
     4501 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4561 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4621 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4681 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4741 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4801 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4861 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4921 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4981 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5041 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5101 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5161 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gagctcgcga
     5221 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     5281 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     5341 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta
     5401 ccggcagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
     5461 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5521 aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5581 gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5641 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5701 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5761 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5821 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5881 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5941 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6001 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6061 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6121 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6181 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6241 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6301 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     6361 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6421 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6481 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6541 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6601 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6661 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6721 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6781 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6841 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6901 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6961 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7021 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7081 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7141 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7201 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7261 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7321 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7381 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7441 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7501 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7561 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7621 aaaagtgcca cctgggtcct tttcatcacg tgctataaaa ataattataa tttaaatttt
     7681 ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt
     7741 ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat
     7801 cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga
     7861 ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat
     7921 ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt
     7981 ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact
     8041 ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat
     8101 tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg tcctaagaaa
     8161 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
