LOCUS       Exported                8040 bp ds-DNA     circular SYN 24-NOV-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 9, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Kpn1-TPI-promoter-Xho1-EGFP-BamH1 in 416
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2010..3006
                     /label=-1000
     misc_feature    3573..4289
                     /label=EGFP coding
     misc_feature    complement(4296..4313)
                     /label=GAGA linker
     misc_feature    complement(4314..5033)
                     /label=EGFP
     primer_bind     complement(5264..5283)
                     /label=T3
     primer_bind     complement(5301..5321)
                     /label=M13-rev
     misc_binding    complement(5327..5349)
                     /label=LacO
     rep_origin      complement(5689..6371)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6469..7128)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtacca tgtccagtat ttactctacc ttgattcctt
     2041 ctcctgaact tcatttccta agccctagtt tcactccatt tacatcagat tatattcacg
     2101 atgatatagc gcataaaggc cattccagtt atgacgtcat gttagattta ttagaccctt
     2161 ccaattctct tgtctctacc gcgatgaaca atccaacata ttttaacgtt tataatacaa
     2221 tcattggtaa tgtagaacct cgtcagatat cgcgtgctat gaccaaatta caacaacgca
     2281 tcaaattccc ctcatggtcc tcttctgcga tgcacgttaa cattggaaga agatcgccat
     2341 acttgccttt acaaccaaat gaaaacgaag ttagcggcat gatgttaagc aatatgtcta
     2401 ccgtggtgaa cgtctttgag aatgcgtgca atacttttga caaagtattt gccaagggcg
     2461 catttttaaa taattataat gtaggcgact tgttccaatc aatgcagaac gttcaagatg
     2521 aattcgccga gtcaagagaa gtagtacaaa gcctgatgga ggattatgta gctgcagaac
     2581 aagattcgta cttggacgat gtacttgttg atgatgaaaa tatggttggc gagttggaag
     2641 aggacctgga tgccgacggt gatcataaat tagtataatg atgccttcct tgttcaggaa
     2701 ttttaccacc gcccaatagt tttattatag tactctcaat atatctatac caacaaccgc
     2761 ttcaaatgta ttatctccct ttataaattc tgcactgtat tgtaatatgt ataaagctct
     2821 gtaccacttt ttcatcattg catacatcga tagcgcctct tcaactttag aatttcctaa
     2881 agggtagaaa aaccagatga ttcaactcag cgtaatggaa cgctctgttt gaaataaaaa
     2941 acatctgtat cgctgcacgg attagttcga taattcaacg acagttatac taatcttggt
     3001 gatggaatga gtactttagc ggaagtaagt ttttgaatta tgcccccact ttttttttgt
     3061 tcatggtgac taacatgaat taggtttata ctattattga ggacgccgaa caggaatgtc
     3121 gaaaaggaga ttttacaaat gcaaaagcga agtatcaaga agccattgaa gttttaggtc
     3181 cgcagaacga gaatttgtct caaaataaat taagttctga cgttacgcaa gccattgacc
     3241 tattaaaaca ggacattaca gccaaaatcc aagaattgga acttttgatt gaaaagcagt
     3301 catcagaaga aaataatatt ggaatggtca acaacaatat gcttattggc tctgttattt
     3361 taaacaacaa atctccgata aatggaataa gcaatgctag gaattgggat aacccagcat
     3421 atcaggacac ttttaaagaa tatggtatga aaattgacga aattacgaaa gaaaataaaa
     3481 aattggctaa tgaaattggg cgattaagag aacgctggga cagcttagta gaaagtgcga
     3541 agcaaagaag ggataagcaa aaaaacgtcg agatggtgag caagggcgag gagctgttca
     3601 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3661 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3721 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     3781 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3841 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3901 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3961 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4021 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4081 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4141 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     4201 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4261 tcactctcgg catggacgag ctgtacaagg gatccggagc aggggcgggt gctatggtga
     4321 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4381 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4441 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4501 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4561 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4621 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4681 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4741 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4801 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4861 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4921 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4981 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taagagctcg
     5041 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
     5101 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
     5161 tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct
     5221 ctaccggcag cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt
     5281 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5341 cacaattcca cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5401 agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5461 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5521 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5581 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5641 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5701 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5761 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5821 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5881 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5941 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6001 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6061 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6121 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6181 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6241 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6301 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6361 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6421 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6481 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6541 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6601 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6661 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6721 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6781 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6841 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6901 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6961 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7021 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7081 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7141 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7201 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7261 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7321 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7381 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7441 ccgaaaagtg ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat
     7501 tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat
     7561 agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt
     7621 tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga
     7681 agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc
     7741 tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat
     7801 tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt
     7861 actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat
     7921 tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtcctaag
     7981 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
