LOCUS       Exported                8133 bp ds-DNA     circular SYN 28-NOV-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 9, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Kpn1-TPI-promoter-Xho1-EGFP-BamH1 in 416
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2010..3006
                     /label=-1000
     misc_feature    3666..4382
                     /label=EGFP coding
     misc_feature    complement(4389..4406)
                     /label=GAGA linker
     misc_feature    complement(4407..5126)
                     /label=EGFP
     primer_bind     complement(5357..5376)
                     /label=T3
     primer_bind     complement(5394..5414)
                     /label=M13-rev
     misc_binding    complement(5420..5442)
                     /label=LacO
     rep_origin      complement(5782..6464)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6562..7221)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtacca tgtccagtat ttactctacc ttgattcctt
     2041 ctcctgaact tcatttccta agccctagtt tcactccatt tacatcagat tatattcacg
     2101 atgatatagc gcataaaggc cattccagtt atgacgtcat gttagattta ttagaccctt
     2161 ccaattctct tgtctctacc gcgatgaaca atccaacata ttttaacgtt tataatacaa
     2221 tcattggtaa tgtagaacct cgtcagatat cgcgtgctat gaccaaatta caacaacgca
     2281 tcaaattccc ctcatggtcc tcttctgcga tgcacgttaa cattggaaga agatcgccat
     2341 acttgccttt acaaccaaat gaaaacgaag ttagcggcat gatgttaagc aatatgtcta
     2401 ccgtggtgaa cgtctttgag aatgcgtgca atacttttga caaagtattt gccaagggcg
     2461 catttttaaa taattataat gtaggcgact tgttccaatc aatgcagaac gttcaagatg
     2521 aattcgccga gtcaagagaa gtagtacaaa gcctgatgga ggattatgta gctgcagaac
     2581 aagattcgta cttggacgat gtacttgttg atgatgaaaa tatggttggc gagttggaag
     2641 aggacctgga tgccgacggt gatcataaat tagtataatg atgccttcct tgttcaggaa
     2701 ttttaccacc gcccaatagt tttattatag tactctcaat atatctatac caacaaccgc
     2761 ttcaaatgta ttatctccct ttataaattc tgcactgtat tgtaatatgt ataaagctct
     2821 gtaccacttt ttcatcattg catacatcga tagcgcctct tcaactttag aatttcctaa
     2881 agggtagaaa aaccagatga ttcaactcag cgtaatggaa cgctctgttt gaaataaaaa
     2941 acatctgtat cgctgcacgg attagttcga taattcaacg acagttatac taatcttggt
     3001 gatggaatga gtactttagc ggaagtaagt ttttgaatta tgcccccact ttttttttgt
     3061 tcatggtgac taacatgaat taggtttata ctattattga ggacgccgaa caggaatgtc
     3121 gaaaaggaga ttttacaaat gcaaaagcga agtatcaaga agccattgaa gttttaggtc
     3181 cgcagaacga gaatttgtct caaaataaat taagttctga cgttacgcaa gccattgacc
     3241 tattaaaaca ggacattaca gccaaaatcc aagaattgga acttttgatt gaaaagcagt
     3301 catcagaaga aaataatatt ggaatggtca acaacaatat gcttattggc tctgttattt
     3361 taaacaacaa atctccgata aatggaataa gcaatgctag gaattgggat aacccagcat
     3421 atcaggacac tttaagtccc ataaacgatc cacttcttat gtcaatttta aaccgcttac
     3481 aatttaattt gaataatgat attcaattga aaacagaagg aggaaaaaat tccaaaaact
     3541 cggaaatgaa gattaactta agactggaac aattcaaaaa ggagttggta ttatatgagc
     3601 agaaaaaatg ggacagctta gtagaaagtg cgaagcaaag aagggataag caaaaaaacg
     3661 tcgagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3721 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3781 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3841 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3901 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3961 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     4021 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     4081 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     4141 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     4201 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4261 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     4321 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4381 agggatccgg agcaggggcg ggtgctatgg tgagcaaggg cgaggagctg ttcaccgggg
     4441 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     4501 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     4561 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     4621 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4681 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4741 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4801 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     4861 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     4921 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4981 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     5041 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5101 tcggcatgga cgagctgtac aagtaagagc tcgcgaattt cttatgattt atgattttta
     5161 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     5221 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     5281 tatagcatga ggtcgctctt attgaccaca cctctaccgg cagcggccgc caccgcggtg
     5341 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     5401 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca taggagccgg
     5461 aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt
     5521 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5581 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5641 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5701 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5761 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5821 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5881 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5941 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6001 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6061 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6241 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6601 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6841 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6961 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7021 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7141 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7321 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7381 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtccttttc
     7561 atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa
     7621 atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag
     7681 actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc tctcaggtat
     7741 taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt
     7801 tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata
     7861 aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt
     7921 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
     7981 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
     8041 cgtgtaagtt acaggcaagc gatccgtcct aagaaaccat tattatcatg acattaacct
     8101 ataaaaatag gcgtatcacg aggccctttc gtc
//
