LOCUS       Exported                7956 bp ds-DNA     circular SYN 10-DEC-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 9, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Kpn1-TPI-promoter-Xho1-EGFP-BamH1 in 416
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2010..3006
                     /label=-1000
     misc_feature    3489..4205
                     /label=EGFP coding
     misc_feature    complement(4212..4229)
                     /label=GAGA linker
     misc_feature    complement(4230..4949)
                     /label=EGFP
     primer_bind     complement(5180..5199)
                     /label=T3
     primer_bind     complement(5217..5237)
                     /label=M13-rev
     misc_binding    complement(5243..5265)
                     /label=LacO
     rep_origin      complement(5605..6287)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6385..7044)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtacca tgtccagtat ttactctacc ttgattcctt
     2041 ctcctgaact tcatttccta agccctagtt tcactccatt tacatcagat tatattcacg
     2101 atgatatagc gcataaaggc cattccagtt atgacgtcat gttagattta ttagaccctt
     2161 ccaattctct tgtctctacc gcgatgaaca atccaacata ttttaacgtt tataatacaa
     2221 tcattggtaa tgtagaacct cgtcagatat cgcgtgctat gaccaaatta caacaacgca
     2281 tcaaattccc ctcatggtcc tcttctgcga tgcacgttaa cattggaaga agatcgccat
     2341 acttgccttt acaaccaaat gaaaacgaag ttagcggcat gatgttaagc aatatgtcta
     2401 ccgtggtgaa cgtctttgag aatgcgtgca atacttttga caaagtattt gccaagggcg
     2461 catttttaaa taattataat gtaggcgact tgttccaatc aatgcagaac gttcaagatg
     2521 aattcgccga gtcaagagaa gtagtacaaa gcctgatgga ggattatgta gctgcagaac
     2581 aagattcgta cttggacgat gtacttgttg atgatgaaaa tatggttggc gagttggaag
     2641 aggacctgga tgccgacggt gatcataaat tagtataatg atgccttcct tgttcaggaa
     2701 ttttaccacc gcccaatagt tttattatag tactctcaat atatctatac caacaaccgc
     2761 ttcaaatgta ttatctccct ttataaattc tgcactgtat tgtaatatgt ataaagctct
     2821 gtaccacttt ttcatcattg catacatcga tagcgcctct tcaactttag aatttcctaa
     2881 agggtagaaa aaccagatga ttcaactcag cgtaatggaa cgctctgttt gaaataaaaa
     2941 acatctgtat cgctgcacgg attagttcga taattcaacg acagttatac taatcttggt
     3001 gatggaatga gtactttagc ggaagtaagt ttttgaatta tgcccccact ttttttttgt
     3061 tcatggtgac taacatgaat taggtttata ctattattga ggacgccgaa caggaatgtc
     3121 gaaaaggaga ttttacaaat gcaaaagcga agtatcaaga agccattgaa gttttaggtc
     3181 cgcagaacga gaatttgtct caaaataaat taagttctga cgttacgcaa gccattgacc
     3241 tattaaaaca ggacattaca gccaaaatcc aagaattgga acttttgatt gaaaagcagt
     3301 catcagaaga aaataatatt ggaatggtca acaacaatat gcttattggc tctgttattt
     3361 taaacaacaa atctccgata aatggaataa gcaatgctag gaattgggat aacccagcat
     3421 atcaggacac ttgggacagc ttagtagaaa gtgcgaagca aagaagggat aagcaaaaaa
     3481 acgtcgagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3541 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     3601 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     3661 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     3721 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     3781 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     3841 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     3901 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     3961 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     4021 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     4081 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     4141 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     4201 acaagggatc cggagcaggg gcgggtgcta tggtgagcaa gggcgaggag ctgttcaccg
     4261 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     4321 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     4381 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     4441 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     4501 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4561 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4621 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     4681 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     4741 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4801 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     4861 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4921 ctctcggcat ggacgagctg tacaagtaag agctcgcgaa tttcttatga tttatgattt
     4981 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     5041 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     5101 aggtatagca tgaggtcgct cttattgacc acacctctac cggcagcggc cgccaccgcg
     5161 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     5221 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acataggagc
     5281 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc
     5341 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5401 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5461 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5521 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5581 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5641 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5701 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5761 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5821 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5881 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5941 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6001 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6061 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6121 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6181 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6241 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6301 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6361 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6421 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6481 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6541 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6601 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6661 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6721 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6781 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6841 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6901 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6961 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7021 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7081 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7141 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7201 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7261 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7321 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcctt
     7381 ttcatcacgt gctataaaaa taattataat ttaaattttt taatataaat atataaatta
     7441 aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa
     7501 aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc tgctctcagg
     7561 tattaatgcc gaattgtttc atcttgtctg tgtagaagac cacacacgaa aatcctgtga
     7621 ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt ttcttgtcta
     7681 ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg tttatttttt
     7741 tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc aaatacaaaa
     7801 cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa aagatacgag
     7861 gcgcgtgtaa gttacaggca agcgatccgt cctaagaaac cattattatc atgacattaa
     7921 cctataaaaa taggcgtatc acgaggccct ttcgtc
//
