LOCUS       Exported                8166 bp ds-DNA     circular SYN 12-DEC-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 9, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Kpn1-TPI-promoter-Xho1-EGFP-BamH1 in 416
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2010..3006
                     /label=-1000
     misc_feature    3699..4415
                     /label=EGFP coding
     misc_feature    complement(4422..4439)
                     /label=GAGA linker
     misc_feature    complement(4440..5159)
                     /label=EGFP
     primer_bind     complement(5390..5409)
                     /label=T3
     primer_bind     complement(5427..5447)
                     /label=M13-rev
     misc_binding    complement(5453..5475)
                     /label=LacO
     rep_origin      complement(5815..6497)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6595..7254)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtacca tgtccagtat ttactctacc ttgattcctt
     2041 ctcctgaact tcatttccta agccctagtt tcactccatt tacatcagat tatattcacg
     2101 atgatatagc gcataaaggc cattccagtt atgacgtcat gttagattta ttagaccctt
     2161 ccaattctct tgtctctacc gcgatgaaca atccaacata ttttaacgtt tataatacaa
     2221 tcattggtaa tgtagaacct cgtcagatat cgcgtgctat gaccaaatta caacaacgca
     2281 tcaaattccc ctcatggtcc tcttctgcga tgcacgttaa cattggaaga agatcgccat
     2341 acttgccttt acaaccaaat gaaaacgaag ttagcggcat gatgttaagc aatatgtcta
     2401 ccgtggtgaa cgtctttgag aatgcgtgca atacttttga caaagtattt gccaagggcg
     2461 catttttaaa taattataat gtaggcgact tgttccaatc aatgcagaac gttcaagatg
     2521 aattcgccga gtcaagagaa gtagtacaaa gcctgatgga ggattatgta gctgcagaac
     2581 aagattcgta cttggacgat gtacttgttg atgatgaaaa tatggttggc gagttggaag
     2641 aggacctgga tgccgacggt gatcataaat tagtataatg atgccttcct tgttcaggaa
     2701 ttttaccacc gcccaatagt tttattatag tactctcaat atatctatac caacaaccgc
     2761 ttcaaatgta ttatctccct ttataaattc tgcactgtat tgtaatatgt ataaagctct
     2821 gtaccacttt ttcatcattg catacatcga tagcgcctct tcaactttag aatttcctaa
     2881 agggtagaaa aaccagatga ttcaactcag cgtaatggaa cgctctgttt gaaataaaaa
     2941 acatctgtat cgctgcacgg attagttcga taattcaacg acagttatac taatcttggt
     3001 gatggaatga gtactttagc ggaagtaagt ttttgaatta tgcccccact ttttttttgt
     3061 tcatggtgac taacatgaat taggtttata ctattattga ggacgccgaa caggaatgtc
     3121 gaaaaggaga ttttacaaat gcaaaagcga agtatcaaga agccattgaa gttttaggtc
     3181 cgcagaacga gaatttgtct caaaataaat taagttctga cgttacgcaa gccattgacc
     3241 tattaaaaca ggacattaca gccaaaatcc aagaattgga acttttgatt gaaaagcagt
     3301 catcagaaga aaataatatt ggaatggtca acaacaatat gcttattggc tctgttattt
     3361 taaacaacaa atctccgata aatggaataa gcaatgctag gaattgggat aacccagcat
     3421 atcaggacac tttaagtccc ataaacgatc cacttcttat gtcaatttta aaccgcttac
     3481 aatttaattt gaataatgat attcaattga aaacagaagg aggaaaaaat tccaaaaact
     3541 cggaaatgaa gattaactta agactggaac aattcaaaaa ggagttggta ttatatgagc
     3601 agaaaaaatt taaagaatat ggtatgaaaa ttgacgaaat tacgaaagaa aataaaaaat
     3661 tggctaatga aattgggcga ttaagagaac gcgtcgagat ggtgagcaag ggcgaggagc
     3721 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3781 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3841 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     3901 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3961 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     4021 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     4081 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     4141 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     4201 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     4261 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     4321 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     4381 ccgggatcac tctcggcatg gacgagctgt acaagggatc cggagcaggg gcgggtgcta
     4441 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4501 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4561 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4621 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     4681 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4741 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4801 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4861 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4921 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4981 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     5041 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     5101 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     5161 agctcgcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
     5221 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
     5281 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc
     5341 acacctctac cggcagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
     5401 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5461 tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag cctggggtgc
     5521 ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5581 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5641 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5821 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6001 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6241 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     6301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6481 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6541 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6601 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6661 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6721 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6781 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6841 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6901 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6961 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7021 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7081 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7141 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7201 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7261 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7321 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7381 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7441 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7501 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7561 atttccccga aaagtgccac ctgggtcctt ttcatcacgt gctataaaaa taattataat
     7621 ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga
     7681 aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact
     7741 accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg
     7801 tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg
     7861 tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt
     7921 tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct
     7981 ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc
     8041 ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca agcgatccgt
     8101 cctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     8161 ttcgtc
//
