LOCUS       Exported                6547 bp ds-DNA     circular SYN 12-NOV-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 9, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2010..3006
                     /label=-1000
     misc_feature    3027..3082
                     /label=intron
     misc_feature    3435..3500
                     /label=x3FLAG
     misc_feature    3504..3736
                     /label=ADH1 terminator
     primer_bind     complement(3771..3790)
                     /label=T3
     primer_bind     complement(3808..3828)
                     /label=M13-rev
     misc_binding    complement(3834..3856)
                     /label=LacO
     rep_origin      complement(4196..4878)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(4976..5635)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtacca tgtccagtat ttactctacc ttgattcctt
     2041 ctcctgaact tcatttccta agccctagtt tcactccatt tacatcagat tatattcacg
     2101 atgatatagc gcataaaggc cattccagtt atgacgtcat gttagattta ttagaccctt
     2161 ccaattctct tgtctctacc gcgatgaaca atccaacata ttttaacgtt tataatacaa
     2221 tcattggtaa tgtagaacct cgtcagatat cgcgtgctat gaccaaatta caacaacgca
     2281 tcaaattccc ctcatggtcc tcttctgcga tgcacgttaa cattggaaga agatcgccat
     2341 acttgccttt acaaccaaat gaaaacgaag ttagcggcat gatgttaagc aatatgtcta
     2401 ccgtggtgaa cgtctttgag aatgcgtgca atacttttga caaagtattt gccaagggcg
     2461 catttttaaa taattataat gtaggcgact tgttccaatc aatgcagaac gttcaagatg
     2521 aattcgccga gtcaagagaa gtagtacaaa gcctgatgga ggattatgta gctgcagaac
     2581 aagattcgta cttggacgat gtacttgttg atgatgaaaa tatggttggc gagttggaag
     2641 aggacctgga tgccgacggt gatcataaat tagtataatg atgccttcct tgttcaggaa
     2701 ttttaccacc gcccaatagt tttattatag tactctcaat atatctatac caacaaccgc
     2761 ttcaaatgta ttatctccct ttataaattc tgcactgtat tgtaatatgt ataaagctct
     2821 gtaccacttt ttcatcattg catacatcga tagcgcctct tcaactttag aatttcctaa
     2881 agggtagaaa aaccagatga ttcaactcag cgtaatggaa cgctctgttt gaaataaaaa
     2941 acatctgtat cgctgcacgg attagttcga taattcaacg acagttatac taatcttggt
     3001 gatggaatga gtactttagc ggaagtaagt ttttgaatta tgcccccact tttttgttca
     3061 tggtgactaa catgaattag gtttatacta ttattgagga cgccgaacag gaatgtcgaa
     3121 aaggagattt tacaaatgca aaagcgaagt atcaagaagc cattgaagtt ttaggtccgc
     3181 agaacgagaa tttgtctcaa aataaattaa gttctgacgt tacgcaagcc attgacctat
     3241 taaaacagga cattacagcc aaaatccaag aattggaact tttgattgaa aagcagtcat
     3301 cagaagaaaa taatattgga atggtcaaca acaatatgct tattggctct gttattttaa
     3361 acaacaaatc tccgataaat ggaataagca atgctaggaa ttgggataac ccagcatatc
     3421 aggacactgt cgaggactac aaagaccatg acggtgatta taaagatcat gatatcgatt
     3481 acaaggatga cgatgacaag tagaattcgt gagctcgcga atttcttatg atttatgatt
     3541 tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag
     3601 gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct
     3661 caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc gagcaaatgc
     3721 ctgcaaatcg ctccccgcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     3781 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3841 atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg
     3901 cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5941 catttccccg aaaagtgcca cctgggtcct tttcatcacg tgctataaaa ataattataa
     6001 tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag
     6061 aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac
     6121 taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct
     6181 gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat
     6241 gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg
     6301 ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc
     6361 tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt
     6421 cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg
     6481 tcctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     6541 tttcgtc
//
