LOCUS       Exported                3681 bp ds-DNA     circular SYN 27-APR-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 9, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     203..222
                     /label=T7
     misc_feature    311..316
                     /label=RBS (ribosome Binding site)
     misc_feature    328..987
                     /label=GST tag
     misc_feature    939..961
                     /label=pGEX5'for
     misc_feature    988..999
                     /label=tag linker
     misc_feature    1000..1020
                     /label=TEV cleavage site (ENLYFQ//G)
     misc_feature    1393..1424
                     /label=3' generic for infusion
     misc_feature    complement(1472..1494)
                     /label=T7R (T7 terminator primer)
     misc_feature    1779..2639
                     /label=AmpR(w/PstI)
     misc_feature    2810..3398
                     /label=pMB1 ori (from pBR322, without ROP gene)
     misc_feature    complement(2849..2956)
                     /label=RNAI
ORIGIN
        1 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
       61 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      121 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
      181 caggatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc
      241 tctagtgccg gctccggaga gctccaattg gaattcgcta gctctagaaa taattttgtt
      301 taactttaag aaggagatat acataatatg tcccctatac taggttattg gaaaattaag
      361 ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat
      421 ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag
      481 tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc
      541 atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag
      601 atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat
      661 agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa
      721 atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct
      781 gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat
      841 gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag
      901 tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt
      961 ggtggcgacc atcctccaaa atcggattct tctggttccg aaaacctgta tttccagggc
     1021 tcccatatga gtactttagc ggaagtttat actattattg aggacgccga acaggaatgt
     1081 cgaaaaggag attttacaaa tgcaaaagcg aagtatcaag aagccattga agttttaggt
     1141 ccgcagaacg agaatttgtc tcaaaataaa ttaagttctg acgttacgca agccattgac
     1201 ctattaaaac aggacattac agccaaaatc caagaattgg aacttttgat tgaaaagcag
     1261 tcatcagaag aaaataatat tggaatggtc aacaacaata tgcttattgg ctctgttatt
     1321 ttaaacaaca aatctccgat aaatggaata agcaatgcta ggaattggga taacccagca
     1381 tatcaggaca cttagggatc cagatctaag cttggtacca cgcgtgcgcg ctgatccggc
     1441 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     1501 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     1561 agccggataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     1621 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1681 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1741 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1801 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1861 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1921 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1981 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     2041 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2101 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2161 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2221 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2281 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     2341 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2401 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2461 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2521 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2581 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2641 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2701 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2761 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2821 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2881 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2941 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3001 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3061 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3121 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3181 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3241 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3301 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3361 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3421 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3481 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3541 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     3601 tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc
     3661 tgatgccgca tagttaagcc a
//
