LOCUS       Exported                3816 bp ds-DNA     circular SYN 28-APR-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 9, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     203..222
                     /label=T7
     misc_feature    311..316
                     /label=RBS (ribosome Binding site)
     misc_feature    328..987
                     /label=GST tag
     misc_feature    939..961
                     /label=pGEX5'for
     misc_feature    988..999
                     /label=tag linker
     misc_feature    1000..1020
                     /label=TEV cleavage site (ENLYFQ//G)
     misc_feature    1393..1416
                     /label=yoo740
     misc_feature    complement(1607..1629)
                     /label=T7R (T7 terminator primer)
     misc_feature    1914..2774
                     /label=AmpR(w/PstI)
     misc_feature    2945..3533
                     /label=pMB1 ori (from pBR322, without ROP gene)
     misc_feature    complement(2984..3091)
                     /label=RNAI
ORIGIN
        1 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
       61 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      121 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
      181 caggatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc
      241 tctagtgccg gctccggaga gctccaattg gaattcgcta gctctagaaa taattttgtt
      301 taactttaag aaggagatat acataatatg tcccctatac taggttattg gaaaattaag
      361 ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat
      421 ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag
      481 tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc
      541 atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag
      601 atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat
      661 agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa
      721 atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct
      781 gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat
      841 gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag
      901 tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt
      961 ggtggcgacc atcctccaaa atcggattct tctggttccg aaaacctgta tttccagggc
     1021 tcccatatga gtactttagc ggaagtttat actattattg aggacgccga acaggaatgt
     1081 cgaaaaggag attttacaaa tgcaaaagcg aagtatcaag aagccattga agttttaggt
     1141 ccgcagaacg agaatttgtc tcaaaataaa ttaagttctg acgttacgca agccattgac
     1201 ctattaaaac aggacattac agccaaaatc caagaattgg aacttttgat tgaaaagcag
     1261 tcatcagaag aaaataatat tggaatggtc aacaacaata tgcttattgg ctctgttatt
     1321 ttaaacaaca aatctccgat aaatggaata agcaatgcta ggaattggga taacccagca
     1381 tatcaggaca cttttaaaga atatggtatg aaaattgacg aaattacgaa agaaaataaa
     1441 aaattggcta atgaaattgg gcgattaaga gaacgctggg acagcttagt agaaagtgcg
     1501 aagcaaagaa gggataagca aaaaaactag ggatccagat ctaagcttgg taccacgcgt
     1561 gcgcgctgat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     1621 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     1681 gaaaggagga actatagccg gataattctt gaagacgaaa gggcctcgtg atacgcctat
     1741 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1801 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1861 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1921 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1981 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2041 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2101 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     2161 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2221 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2281 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2341 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2401 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     2461 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2521 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2581 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2641 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2701 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2761 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2821 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2881 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2941 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3001 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3061 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3301 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3601 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3661 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3721 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct
     3781 cagtacaatc tgctctgatg ccgcatagtt aagcca
//
