LOCUS       Exported                9858 bp ds-DNA     circular SYN 28-OCT-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 9, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..756
                     /label=CMV promoter
     misc_feature    759..761
                     /label=start
     misc_feature    762..776
                     /label=GAGA linker
     misc_feature    777..1175
                     /label=proteinA tag x2
                     /label=proteinA tag x2(1)
     misc_feature    1176..1196
                     /label=TEV cleavage site
     misc_feature    1227..1247
                     /label=TEV
     misc_feature    1269..1289
                     /label=TEV
                     /label=TEV(3)
     misc_feature    1344..1346
                     /label=start
                     /label=start(2)
     misc_feature    1350..2822
                     /label=ATG14L N256S
     misc_feature    2113
                     /label=mutation1
     misc_feature    2829..2831
                     /label=stop
     misc_feature    2884..3108
                     /label=PolyA
     misc_feature    3109..3472
                     /label=CMV enhencer
     misc_feature    3473..3754
                     /label=Chicken b-actin promoter
     misc_feature    3755..4715
                     /label=intron chicken b-actin
     misc_feature    4726..4770
                     /label=intron rabbit b-globin
     misc_feature    4814..4849
                     /label=5' infusion generic
     misc_feature    4850..4852
                     /label=start
                     /label=start(1)
     misc_feature    4856..4870
                     /label=GAGA linker
                     /label=GAGA linker(1)
     misc_feature    4871..5269
                     /label=proteinA tag x2
     misc_feature    5270..5290
                     /label=TEV cleavage site
                     /label=TEV cleavage site(1)
     misc_feature    5321..5341
                     /label=TEV
                     /label=TEV(1)
     misc_feature    5363..5383
                     /label=TEV
                     /label=TEV(2)
     misc_feature    join(5438..6744,6746..6756,6758..6771,6773..6790)
                     /label=Beclin FL
     misc_feature    6791..6820
                     /label=3' infusion generi
     misc_feature    6800..7326
                     /label=polyA-rabbit b-globin
     primer_bind     complement(7357..7377)
                     /label=M13-rev
     misc_binding    complement(7383..7405)
                     /label=LacO
     misc_feature    8093..8712
                     /label=pBR322origin
     misc_feature    8550..8657
                     /label=RNAI
     misc_feature    complement(8867..9529)
                     /label=AmpR
ORIGIN
        1 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggaaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
      601 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      661 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      721 ctccggactc tagcgtttaa acttaagctt ggtaccggat gggagcaggg gcgggtgcta
      781 gcgagaacct gtacttccag ggcgcccacg atgaagccgt agacaacaaa ttcaacaaag
      841 aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa gaacaacgaa
      901 acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt ttagcagaag
      961 ctaaaaagct aaatgatgct caggcgccga aagtagacaa caaattcaac aaagaacaac
     1021 aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct
     1081 tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca gaagctaaaa
     1141 agctaaatga tgctcaggcg ccgaaagtag acgcggaaaa cctgtatttc cagggaggcg
     1201 gatcttctag accaactact gcctctgaaa atttgtactt tcaaggcgag ttgaagacca
     1261 ctgcttctga aaacttgtac ttccaaggtg agttactcga gggtgaattg aagactgctg
     1321 ctttggctca acacgacgaa gccatgacgg cgtctcccag tgggaaggga gcccgggcgc
     1381 tggaggctcc tggctgcggg ccccggccgc tcgcccggga cctggtggac tccgtggacg
     1441 atgcggaggg gctgtacgtg gctgtggagc gctgcccgct gtgcaacact acccgccggc
     1501 ggctgacctg cgccaaatgc gttcagagcg gcgatttcgt ctacttcgac ggccgcgacc
     1561 gggagaggtt tatcgacaag aaggaaaggt taagccgact taagagcaag caagaagaat
     1621 ttcagaaaga agtgttaaaa gctatggaag gaaaatggat aacagatcag ttgagatgga
     1681 aaataatgtc ctgcaagatg aggattgaac agttaaaaca aacaatatgt aaaggaaatg
     1741 aagaaatgga gaaaaattct gaaggccttc tcaaaaccaa ggaaaagaat cagaagcttt
     1801 acagtcgagc acaacggcac caagagaaaa aggagaagat tcagaggcat aatcgcaaac
     1861 ttggtgacct ggtagaaaaa aagaccattg acttaagaag tcattatgag cgtctggcaa
     1921 atcttcgacg atcccatata ttagagctca cctctgtcat ttttccaatc gaggaagtaa
     1981 agacgggtgt gagagacccc gcagatgtgt cttcagagag tgacagtgcc atgacctcca
     2041 gcactgtgag caagcttgct gaagcccgga ggacaactta cctctcagga cgatgggtct
     2101 gtgacgatca cagcggagac accagcatta gcattacagg gccttggatt agcctcccta
     2161 acaatgggga ctactctgcc tactacagct gggtggagga gaagaaaaca acccaggggc
     2221 ctgacatgga gcagagtaac cctgcctaca ccatcagtgc tgcgctgtgc tatgcaactc
     2281 agctggtcaa cattctgtct catatacttg atgtaaatct tcccaaaaag ctctgcaaca
     2341 gtgaattttg tggcgaaaat ctaagcaagc agaaatttac tcgagcagtg aagaaactga
     2401 atgcaaatat tctttacctt tgtttttctc agcatgtaaa tttagatcaa ttacaaccac
     2461 tgcataccct caggaatcta atgtacctgg tcagtccaag ctctgaacac ctaggcaggt
     2521 cagggccctt tgaagtacga gcagaccttg aggagtccat ggaatttgtg gatcccggag
     2581 ttgctggaga atcagatgag agcggagatg agcgcgtcag cgatgaagaa accgacctgg
     2641 gcacagactg ggagaacttg cctagtcccc ggttttgtga tatcccttcc cagtctgtgg
     2701 aagtctccca gagtcagagc acccaggcgt ccccacccat cgcgagcagc agtgcaggtg
     2761 ggatgatctc ctctgcagca gcctcggtga cctcctggtt taaagcttac actggacacc
     2821 gtagatccta acccaagctt aagtcgagta gagggcccgt ttaaacccgc tgatcagcct
     2881 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     2941 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     3001 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3061 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggac tagttattaa
     3121 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     3181 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     3241 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     3301 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     3361 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     3421 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
     3481 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
     3541 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc
     3601 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
     3661 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
     3721 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc
     3781 gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     3841 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     3901 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
     3961 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
     4021 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     4081 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
     4141 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
     4201 gcgcgtcggt cgggctgcaa cccccccctg cacccccctc cccgagttgc tgagcacggc
     4261 ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg
     4321 gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg
     4381 ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc
     4441 cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt
     4501 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg
     4561 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     4621 ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga
     4681 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     4741 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     4801 tctcatcatt ttggcaaaga attgctcgat cgacggtatc gatgccacca tgatgggagc
     4861 aggggcgggt gctagcgaga acctgtactt ccagggcgcc cacgatgaag ccgtagacaa
     4921 caaattcaac aaagaacaac aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa
     4981 cgaagaacaa cgaaacgcct tcatccaaag tttaaaagat gacccaagcc aaagcgctaa
     5041 ccttttagca gaagctaaaa agctaaatga tgctcaggcg ccgaaagtag acaacaaatt
     5101 caacaaagaa caacaaaacg cgttctatga gatcttacat ttacctaact taaacgaaga
     5161 acaacgaaac gccttcatcc aaagtttaaa agatgaccca agccaaagcg ctaacctttt
     5221 agcagaagct aaaaagctaa atgatgctca ggcgccgaaa gtagacgcgg aaaacctgta
     5281 tttccaggga ggcggatctt ctagaccaac tactgcctct gaaaatttgt actttcaagg
     5341 cgagttgaag accactgctt ctgaaaactt gtacttccaa ggtgagttac tcgagggtga
     5401 attgaagact gctgctttgg ctcaacacga cgaagccatg gaagggtcta agacgtccaa
     5461 caacagcacc atgcaggtga gcttcgtgtg ccagcgctgc agccagcccc tgaaactgga
     5521 cacgagtttc aagatcctgg accgtgtcac catccaggaa ctcacagctc cattacttac
     5581 cacagcccag gcgaaaccag gagagaccca ggaggaagag actaactcag gagaggagcc
     5641 atttattgaa actcctcgcc aggatggtgt ctctcgcaga ttcatccccc cagccaggat
     5701 gatgtccaca gaaagtgcca acagcttcac tctgattggg gaggcatctg atggcggcac
     5761 catggagaac ctcagccgaa gactgaaggt cactggggac ctttttgaca tcatgtcggg
     5821 ccagacagat gtggatcacc cactctgtga ggaatgcaca gatactcttt tagaccagct
     5881 ggacactcag ctcaacgtca ctgaaaatga gtgtcagaac tacaaacgct gtttggagat
     5941 cttagagcaa atgaatgagg atgacagtga acagttacag atggagctaa aggagctggc
     6001 actagaggag gagaggctga tccaggagct ggaagacgtg gaaaagaacc gcaagatagt
     6061 ggcagaaaat ctcgagaagg tccaggctga ggctgagaga ctggatcagg aggaagctca
     6121 gtatcagaga gaatacagtg aatttaaacg acagcagctg gagctggatg atgagctgaa
     6181 gagtgttgaa aaccagatgc gttatgccca gacgcagctg gataagctga agaaaaccaa
     6241 cgtctttaat gcaaccttcc acatctggca cagtggacag tttggcacaa tcaataactt
     6301 caggctgggt cgcctgccca gtgttcccgt ggaatggaat gagattaatg ctgcttgggg
     6361 ccagactgtg ttgctgctcc atgctctggc caataagatg ggtctgaaat ttcagagata
     6421 ccgacttgtt ccttacggaa accattcata tctggagtct ctgacagaca aatctaagga
     6481 gctgccgtta tactgttctg gggggttgcg gtttttctgg gacaacaagt ttgaccatgc
     6541 aatggtggct ttcctggact gtgtgcagca gttcaaagaa gaggttgaga aaggcgagac
     6601 acgtttttgt cttccctaca ggatggatgt ggagaaaggc aagattgaag acacaggagg
     6661 cagtggcggc tcctattcca tcaaaaccca gtttaactct gaggagcagt ggacaaaagc
     6721 tctcaagttc atgctgacga atctgaagtg gggtctggct tgggtgtcct ctcaatttta
     6781 taacaaatga ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     6841 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     6901 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     6961 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     7021 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     7081 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     7141 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     7201 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     7261 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     7321 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7381 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7441 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7501 tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt
     7561 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7621 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7681 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg
     7741 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7801 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7861 gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7921 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7981 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8041 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8101 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8161 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8221 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8281 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     8341 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8401 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8461 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8521 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8581 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8641 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8701 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8761 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8821 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8881 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8941 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9001 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9061 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9121 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9181 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9241 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9301 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9361 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9421 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9481 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9541 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9601 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9661 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9721 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9781 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9841 cccgaaaagt gccacctg
//
