LOCUS       PD0034_CROPseq-mCherry-MS2       10208 bp    DNA     circular UNA 04-MAR-2019
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...66-bsz22pe
VERSION     urn.local...66-bsz22pe
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(516..1131)
                     /note="pUC Ori"
                     /Color="#aaaaaa"
                     /modified_by="User"
                     /label="pUC Ori"
     primer_bind     1170..1189
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22602678477875fee201c1-f9b3-4636-b1f8-9
                     f4d85d3e184"
                     /%GC="60.0"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.1"
                     /Sequence="CGAGTCAGTGAGCGAGGAAG"
                     /label="86708_p13"
     misc_feature    complement(1192..1198)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /label="SapI"
                     /note="Geneious type: restriction site"
     LTR             1479..1891
                     /note="RSV/5'LTR"
                     /Color="#6bba70"
                     /modified_by="User"
                     /label="5' LTR"
     primer_bind     1645..1664
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="226025523865652ca6a344-0e77-4267-8b57-7
                     2bcc269f02a"
                     /%GC="55.0"
                     /Tm="59.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GACGAACCACTGAATTGCCG"
                     /label="86708_p10"
     primer_bind     1804..1822
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22603136063395f02f6ab4-736c-46f6-9526-3
                     451cef2d4fd"
                     /%GC="52.6"
                     /Tm="57.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTCAAGTAGTGTGTGCCCG"
                     /label="PCR_0119"
     misc_feature    1942..2079
                     /note="psi"
                     /label="psi"
     misc_feature    2046..2410
                     /note="gag"
                     /label="gag"
     primer_bind     2163..2182
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22602594181602a2013eae-c028-49c4-a022-1
                     0273fc7ec65"
                     /%GC="55.0"
                     /Tm="59.9"
                     /Hairpin_Tm="34.0"
                     /Self_Dimer_Tm="5.9"
                     /Sequence="GGGAGCTAGAACGATTCGCA"
                     /label="86708_p11"
     primer_bind     2239..2259
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="226032527105563bd213e9-33fc-4537-8047-b
                     2e28e71b8f9"
                     /%GC="52.4"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACAACCATCCCTTCAGACAGG"
                     /label="PCR_0248"
     misc_feature    complement(2363..2369)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /label="SapI"
                     /note="Geneious type: restriction site"
     misc_feature    2556..2797
                     /note="RRE"
                     /label="RRE"
     primer_bind     2565..2584
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="2260263550295163bd1787-db70-4ecb-8fae-5
                     530be13f833"
                     /%GC="55.0"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTCCTTGGGTTCTTGGGAGC"
                     /label="86708_p12"
     primer_bind     3171..3195
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22602719471493a1adfa10-f400-4394-af60-6
                     5b9a80652af"
                     /%GC="44.0"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAGACAAATGGCAGTATTCATCCAC"
                     /label="86708_p3"
     misc_feature    3198..3315
                     /note="cPPT"
                     /label="cPPT"
     promoter        3374..4632
                     /note="EF-1a"
                     /Color="#f8af3c"
                     /modified_by="User"
                     /label="EF-1a"
     primer_bind     3626..3645
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22602767379757bbe9be5d-24f8-40ae-9bb9-c
                     15694f3d85d"
                     /%GC="55.0"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TAAGTGCAGTAGTCGCCGTG"
                     /label="86708_p4"
     primer_bind     4000..4019
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="226028071316864a45dfc9-307b-4da6-bdb8-7
                     6f69691d5e5"
                     /%GC="50.0"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="4.4"
                     /Sequence="AGTCTTGTAAATGCGGGCCA"
                     /label="86708_p5"
     primer_bind     4483..4502
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="2260284720963534927e14-8aff-4a28-ad99-8
                     15b37bfdf83"
                     /%GC="60.0"
                     /Tm="60.0"
                     /Hairpin_Tm="45.9"
                     /Self_Dimer_Tm="17.9"
                     /Sequence="CACACTGAGTGGGTGGAGAC"
                     /label="86708_p6"
     CDS             4645..>5241
                     /note="Puromycin resistance"
                     /Color="#f2911a"
                     /modified_by="User"
                     /label="Puro"
     primer_bind     4961..4980
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="226028879781279a0e9cb1-0010-40f7-b33a-1
                     7b5d1683a80"
                     /%GC="55.0"
                     /Tm="60.0"
                     /Hairpin_Tm="34.3"
                     /Self_Dimer_Tm="5.2"
                     /Sequence="AGCAACAGATGGAAGGCCTC"
                     /label="86708_p7"
     primer_bind     4961..4980
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22603008977352794d0165-0949-4f31-90c8-f
                     c8033d2c0f8"
                     /%GC="55.0"
                     /Tm="60.0"
                     /Hairpin_Tm="34.3"
                     /Self_Dimer_Tm="5.2"
                     /Sequence="AGCAACAGATGGAAGGCCTC"
                     /label="PCR_0078"
     misc_feature    5242..5307
                     /label="P2A"
                     /note="Geneious type: self-cleaving-linker"
     CDS             5308..>6015
                     /created_by="User"
                     /Transferred_From="gBlock_MS2-NLS-P2A-mCherry2-T2A"
                     /Transferred_Similarity="94.49%"
                     /Color="#c72c6e"
                     /modified_by="User"
                     /label="mCherry2"
     primer_bind     5308..5328
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22602968300153a44e22d6-c845-437a-92d4-5
                     87f7a8e07e7"
                     /%GC="61.9"
                     /Tm="64.5"
                     /Hairpin_Tm="47.5"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATGGTGAGCAAGGGCGAGGAG"
                     /label="PCR_0058"
     primer_bind     5602..5623
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22603213616486ed4268d1-55f6-4cc9-b2f2-e
                     95435ad7d50"
                     /%GC="54.5"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAGAGGGTGATGAACTTCGAGG"
                     /label="PCR_0244"
     primer_bind     5899..5920
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="226030563543800e14c084-47b7-4081-985f-1
                     cdba8e1624a"
                     /%GC="50.0"
                     /Tm="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TACAACGTGGACATCAAGCTGG"
                     /label="PCR_0085"
     misc_RNA        6034..6622
                     /note="WPRE"
                     /Color="#aaaaaa"
                     /modified_by="User"
                     /label="WPRE"
     primer_bind     6394..6413
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="226029271561214c4e3451-6535-46c4-b89b-7
                     b70bc3161e8"
                     /%GC="50.0"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTGGGCACTGACAATTCCGT"
                     /label="86708_p8.1"
     LTR             6693..>6728
                     /note="3' LTR SIN"
                     /Color="#6bba70"
                     /modified_by="User"
                     /label="3' LTR"
     promoter        6778..>6958
                     /note="Human U6 Promoter"
                     /Color="#28255a"
                     /modified_by="User"
                     /label="hU6"
     primer_bind     6778..6800
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="2260249929462162658c80-1210-4b55-b8d1-d
                     8a43a8fc78f"
                     /%GC="52.2"
                     /Tm="60.8"
                     /Hairpin_Tm="46.4"
                     /Self_Dimer_Tm="5.7"
                     /Sequence="GAGGGCCTATTTCCCATGATTCC"
                     /label="86708_p1"
     ligation        6957..6958
                     /label="Ligation"
     promoter        <6957..7022
                     /note="Human U6 Promoter"
                     /Color="#28255a"
                     /modified_by="User"
                     /label="hU6"
     primer_bind     6967..7026
                     /Sequence="GTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTGTGGAA
                     AGGACGAAACACC"
                     /created_by="User"
                     /label="Oligo-FWD-Joung"
     primer_bind     7009..7026
                     /Sequence="TGGAAAGGACGAAACACC"
                     /Hairpin_Tm="None"
                     /Tm="54.8"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="included in oligo"
     misc_binding    7023..>8068
                     /note="Filler (digest with BsmBI to replace filler with
                     sgRNA oligos)"
                     /modified_by="User"
                     /Color="#c5d7f0"
                     /modified_by="User"
                     /Transferred_From="Backbone_fagment_v2_CROPseq-2xMS2-nofli
                     p, Backbone_fragment_v3_PD004, CROPseq-CAR-v3"
                     /Transferred_Similarity="100.00%"
                     /label="Filler gRNA; filler (1,885 bp)"
     origin          7022^7023
                     /label="origin"
     misc_feature    complement(7028..7033)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     primer_bind     7970..7991
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22603096302272e518320c-0de4-413a-b2d5-0
                     54c7c8387f8"
                     /%GC="45.5"
                     /Tm="57.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTTTAGGTTCAGTTCCACTTGG"
                     /label="PCR_0087"
     misc_feature    8069..8074
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     grna            8076..8217
                     /created_by="User"
                     /Color="#385eaa; #cccccc"
                     /modified_by="User"
                     /Transferred_From="61427_lenti-sgRNA(MS2)-zeo,
                     Backbone_fragment_v3_PD004, CROPseq-CAR-v3"
                     /Transferred_Similarity="100.00% -> 98.59%"
                     /label="MS2-gRNA-backbone"
     primer_bind     complement(8076..8135)
                     /Sequence="ATTTTAACTTGCTAGGCCCTGCAGACATGGGTGATCCTCATGTTGGC
                     CTAGCTCTAAAAC"
                     /created_by="User"
                     /label="Oligo-REV-Joung"
     primer_bind     complement(8076..8096)
                     /Sequence="ATGTTGGCCTAGCTCTAAAAC"
                     /Hairpin_Tm="None"
                     /Tm="55.9"
                     /Self_Dimer_Tm="3.3"
                     /%GC="42.9"
                     /created_by="primer3"
                     /label="included in oligo"
     grna            8088..8121
                     /created_by="User"
                     /Color="#000000; #b02b2c"
                     /modified_by="User"
                     /Transferred_From="61427_lenti-sgRNA(MS2)-zeo,
                     Backbone_fragment_v3_PD004, CROPseq-CAR-v3"
                     /Transferred_Similarity="100.00%"
                     /label="MS2"
     grna            8158..8191
                     /created_by="User"
                     /Color="#000000; #b02b2c"
                     /modified_by="User"
                     /Transferred_From="61427_lenti-sgRNA(MS2)-zeo,
                     Backbone_fragment_v3_PD004, CROPseq-CAR-v3"
                     /Transferred_Similarity="100.00%"
                     /label="MS2"
     LTR             <8260..8459
                     /note="3' LTR SIN"
                     /Color="#6bba70"
                     /modified_by="User"
                     /label="3' LTR"
     primer_bind     8372..8390
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="2260317403378207c5fac6-5dba-4d0d-94b8-c
                     5071970219a"
                     /%GC="52.6"
                     /Tm="57.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTCAAGTAGTGTGTGCCCG"
                     /label="PCR_0119"
     polyA_signal    8536..8666
                     /note="SV40 polyA"
                     /Color="#aaaaaa"
                     /modified_by="User"
                     /label="SV40 polyA"
     gene            9792..>9909
                     /Color="#aaaaaa"
                     /created_by="User"
                     /label="AmpR"
ORIGIN      
        1 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
       61 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      121 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      181 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
      241 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
      301 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
      361 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
      421 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
      481 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      541 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      601 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      661 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      721 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      781 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      841 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      901 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      961 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1021 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     1081 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     1141 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     1201 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1261 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     1321 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     1381 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     1441 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     1501 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     1561 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     1621 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     1681 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     1741 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     1801 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1861 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     1921 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     1981 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     2041 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     2101 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     2161 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     2221 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     2281 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     2341 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     2401 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     2461 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     2521 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     2581 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     2641 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     2701 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     2761 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     2821 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     2881 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     2941 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     3001 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     3061 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     3121 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagaatctt gagacaaatg
     3181 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     3241 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     3301 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc
     3361 gagggggccc gggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     3421 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     3481 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc
     3541 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     3601 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     3661 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     3721 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     3781 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     3841 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     3901 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     3961 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     4021 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     4081 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     4141 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     4201 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     4261 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     4321 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     4381 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     4441 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     4501 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     4561 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     4621 caggtgtcgt gacgtacggc caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4681 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4741 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4801 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc
     4861 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4921 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4981 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc
     5041 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5101 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5161 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5221 atgacccgca agcccggtgc cggatccgga gccacgaact tctctctgtt aaagcaagca
     5281 ggcgacgtgg aagaaaaccc cggtcctatg gtgagcaagg gcgaggagga caacatggcc
     5341 atcatcaagg agttcatgag gttcaaggtg cacatggagg gcagcgtgaa cggccacgag
     5401 ttcgagatcg agggcgaggg cgagggcagg ccctacgagg gcacccagac cgccaagctg
     5461 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgagccc ccagttcatg
     5521 tacggcagca aggcctacgt gaagcacccc gccgacatcc ccgactacct gaagctgagc
     5581 ttccccgagg gcttcaactg ggagagggtg atgaacttcg aggacggcgg cgtggtgacc
     5641 gtgacccagg acagcagcct gcaggacggc gagttcatct acaaggtgaa gctgaggggc
     5701 accaacttcc ccagcgacgg ccccgtgatg cagtgcagga ccatgggctg ggaggccagc
     5761 accgagagga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     5821 ctgaaggacg gcggccacta cgacgccgag gtgaagacca cctacaaggc caagaagccc
     5881 gtgcagctgc ccggcgccta caacgtggac atcaagctgg acatcctgag ccacaacgag
     5941 gactacacca tcgtggagca gtacgagagg gccgagggca ggcacagcac cggcggcatg
     6001 gacgagctgt acaagtgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     6061 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6121 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6181 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6241 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6301 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6361 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6421 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6481 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6541 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6601 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     6661 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6721 gacaagattt caccattatc gtttcagacc cacctcccaa ccccgagggg acccagagag
     6781 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     6841 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     6901 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     6961 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     7021 aacaccggag acggttgtaa atgagcacac aaaatacaca tgctaaaata ttatattcta
     7081 tgacctttat aaaatcaacc aaaatcttct ttttaataac tttagtatca ataattagaa
     7141 tttttatgtt cctttttgca aacttttaat aaaaatgagc aaaataaaaa aacgctagtt
     7201 ttagtaactc gcgttgtttt cttcaccttt aataatagct actccaccac ttgttcctaa
     7261 gcggtcagct cctgcttcaa tcattttttg agcatcttca aatgttctaa ctccaccagc
     7321 tgctttaact aaagcattgt ctttaacaac tgacttcatt agtttaacat cttcaaatgt
     7381 tgcacctgat tttgaaaatc ctgttgatgt tttaacaaat tctaatccag cttcaacagc
     7441 tatttcacaa gctttcatga tttcttcttt tgttaataaa caattttcca taatacattt
     7501 aacaacatgt gatccagctg ctttttttac agctttcatg tcttctaaaa ctaattcata
     7561 atttttgtct tttaatgcac caatatttaa taccatatca atttctgttg caccatcttt
     7621 aattgcttca gaaacttcga atgcttttgt agctgttgtg catgcaccta gaggaaaacc
     7681 tacaacattt gttattccta catttgtgcc ttttaataat tctttacaat agcttgttca
     7741 atatgaatta acacaaactg ttgcaaaatc aaattcaatt gcttcatcac ataattgttt
     7801 aatttcagct ttcgtagcat cttgttttaa taatgtgtga tctatatatt tgtttagttt
     7861 cattttttct cctatatatt catttttaat tttaattctt taataatttc gtctacttta
     7921 actttagcgt tttgaacaga ttcaccaaca cctataaaat aaatttttag tttaggttca
     7981 gttccacttg ggcgaacagc aaatcatgac ttatcttcta aataaaattt tagtaagtct
     8041 tgtcctggca tattatacat tccatcgacg tctctgtttt agagctaggc caacatgagg
     8101 atcacccatg tctgcagggc ctagcaagtt aaaataaggc tagtccgtta tcaacttggc
     8161 caacatgagg atcacccatg tctgcagggc caagtggcac cgagtcggtg cttttttaag
     8221 cttggcgcac caagcttggc gtaactagat cttgagacac tgctttttgc ttgtactggg
     8281 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8341 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8401 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8461 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     8521 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8581 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8641 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccgttc
     8701 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8761 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     8821 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     8881 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8941 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     9001 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9061 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9121 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9181 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9241 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9301 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9361 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9421 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     9481 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9541 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9601 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9661 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9721 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9781 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9841 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9901 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9961 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    10021 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10081 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10141 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10201 cttcccgg
//
