LOCUS       PD0011_Luc_Zeo_humanCodonOptimized       10286 bp    DNA     circular UNA 17-NOV-2017
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...6u-bsz22ph
VERSION     urn.local...6u-bsz22ph
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    24..612
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /label="WPRE"
     misc_feature    complement(77..97)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="2291476385411748374137-fe74-4c4a-b24b-5
                     e86429cdeac"
                     /%GC="42.9"
                     /Tm="56.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CATAGCGTAAAAGGAGCAACA"
                     /label="PCR_0257"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     99..118
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22914815352290a45ac923-3f26-4f2f-8695-2
                     98839c173a6"
                     /%GC="50.0"
                     /Tm="57.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="10.5"
                     /Sequence="GGATACGCTGCTTTAATGCC"
                     /label="PCR_0258"
     CDS             complement(495..506)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
                     /label="Factor Xa site"
     misc_feature    622..627
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     primer_bind     644..665
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22914879012362f5fe98b2-c3eb-4b4f-819d-2
                     fccbf10a57d"
                     /%GC="45.5"
                     /Tm="59.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACATGGAGCAATCACAAGTAGC"
                     /label="PCR_0259"
     misc_feature    775..780
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     primer_bind     996..1016
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="2291494028037413d42fee-3c7b-4202-9d6a-4
                     af4ccdc6753"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AATGAAGGAGAGAACACCCGC"
                     /label="PCR_0260"
     origin          1018^1019
                     /label="origin"
     LTR             1137..1317
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     polyA_signal    1349..1573
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     misc_feature    1593..1598
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     rep_origin      1619..2047
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     rep_origin      2221..2355
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        2417..2464
                     /note="synthetic bacterial promoter"
                     /label="EM7 promoter"
     CDS             2483..2857
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
                     /label="BleoR"
     polyA_signal    2987..3108
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     misc_feature    3124..3129
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     protein_bind    3181..3197
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3205..3235)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3250..3271
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     misc_feature    3320..3325
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     rep_origin      complement(3559..4147)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     CDS             complement(4318..5178)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     promoter        complement(5179..5283)
                     /gene="bla"
                     /label="AmpR promoter"
     misc_feature    5312..5317
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     primer_bind     5369..5389
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="2291444969107638b61729-afbf-4563-874f-4
                     a99900608dd"
                     /%GC="52.4"
                     /Tm="59.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.7"
                     /Sequence="CTGATGCCGCATAGTTAAGCC"
                     /label="PCR_0245"
     enhancer        5549..5928
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     primer_bind     5824..5844
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="229144878214720502b752-962e-4405-bc31-9
                     59b18862e3d"
                     /%GC="52.4"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTCAATGACGGTAAATGGCCC"
                     /label="PCR_0246"
     promoter        5930..6128
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     LTR             6146..6326
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    6373..6498
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /label="HIV-1 Psi"
     primer_bind     6465..6486
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="229145272081494476a0eb-3566-4381-9e3a-5
                     7f597642d3e"
                     /%GC="54.5"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGGCTAGAAGGAGAGAGATGGG"
                     /label="PCR_0247"
     primer_bind     6675..6695
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="229145666557513d2bd87d-c43c-4e3a-a7b3-2
                     e0bb27447ef"
                     /%GC="52.4"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACAACCATCCCTTCAGACAGG"
                     /label="PCR_0248"
     misc_feature    6991..7224
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to
                     thecytoplasm."
                     /label="RRE"
     primer_bind     7268..7288
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22914610682384de143bc3-98f3-4c82-b5b9-1
                     fcf7616fbf6"
                     /%GC="52.4"
                     /Tm="59.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTGCCTTGGAATGCTAGTTGG"
                     /label="PCR_0249"
     primer_bind     7590..7610
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22914663579191437ee27b-7fdb-4eac-a0a5-3
                     69b78dca000"
                     /%GC="52.4"
                     /Tm="59.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TATCGTTTCAGACCCACCTCC"
                     /label="PCR_0250"
     misc_feature    7751..7868
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        7961..8172
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
                     /label="EF-1-alpha core promoter"
     primer_bind     8056..8077
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="229147052405414e236af3-3f03-4dec-b8ef-4
                     2538f884e02"
                     /%GC="50.0"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAAAGTGATGTCGTGTACTGGC"
                     /label="PCR_0251"
     CDS             8197..>9846
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     Optimized_Codon 8200..8202
                     /Relative_Adaptiveness="1.0"
                     /Original="GAA"
                     /label="GAG"
     Optimized_Codon 8215..8217
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATC"
     Optimized_Codon 8227..8229
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     Optimized_Codon 8230..8232
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 8242..8244
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     Optimized_Codon 8260..8262
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     misc_feature    8269..8274
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     Optimized_Codon 8269..8271
                     /Relative_Adaptiveness="1.0"
                     /Original="CAA"
                     /label="CAG"
     Optimized_Codon 8281..8283
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     Optimized_Codon 8302..8304
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 8317..8319
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     Optimized_Codon 8329..8331
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCC"
     Optimized_Codon 8350..8352
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 8356..8358
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     primer_bind     8361..8381
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="22914995024705b20027be-e7e5-45ac-b450-6
                     a4b298c2bfe"
                     /%GC="52.4"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTACTTCGAAATGTCCGTGCG"
                     /label="PCR_0261"
     Optimized_Codon 8377..8379
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 8383..8385
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 8386..8388
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCC"
     Optimized_Codon 8392..8394
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     Optimized_Codon 8401..8403
                     /Relative_Adaptiveness="1.0"
                     /Original="CGA"
                     /label="CGG"
     Optimized_Codon 8431..8433
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 8434..8436
                     /Relative_Adaptiveness="1.0"
                     /Original="GTA"
                     /label="GTG"
     Optimized_Codon 8440..8442
                     /Relative_Adaptiveness="1.0"
                     /Original="AGT"
                     /label="AGC"
     Optimized_Codon 8452..8454
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 8455..8457
                     /Relative_Adaptiveness="1.0"
                     /Original="CAA"
                     /label="CAG"
     Optimized_Codon 8467..8469
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     Optimized_Codon 8473..8475
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 8479..8481
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 8482..8484
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 8494..8496
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 8497..8499
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCC"
     Optimized_Codon 8500..8502
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 8503..8505
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 8509..8511
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 8530..8532
                     /Relative_Adaptiveness="1.0"
                     /Original="CGT"
                     /label="CGG"
     Optimized_Codon 8536..8538
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 8539..8541
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 8545..8547
                     /Relative_Adaptiveness="1.0"
                     /Original="AGT"
                     /label="AGC"
     Optimized_Codon 8557..8559
                     /Relative_Adaptiveness="1.0"
                     /Original="TCG"
                     /label="AGC"
     Optimized_Codon 8578..8580
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 8593..8595
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 8596..8598
                     /Relative_Adaptiveness="1.0"
                     /Original="CAA"
                     /label="CAG"
     Optimized_Codon 8605..8607
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 8614..8616
                     /Relative_Adaptiveness="1.0"
                     /Original="CAA"
                     /label="CAG"
     Optimized_Codon 8623..8625
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 8635..8637
                     /Relative_Adaptiveness="1.0"
                     /Original="CAA"
                     /label="CAG"
     Optimized_Codon 8662..8664
                     /Relative_Adaptiveness="1.0"
                     /Original="ACG"
                     /label="ACC"
     Optimized_Codon 8683..8685
                     /Relative_Adaptiveness="1.0"
                     /Original="TCG"
                     /label="AGC"
     Optimized_Codon 8692..8694
                     /Relative_Adaptiveness="1.0"
                     /Original="ACG"
                     /label="ACC"
     Optimized_Codon 8698..8700
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 8710..8712
                     /Relative_Adaptiveness="1.0"
                     /Original="CTA"
                     /label="CTG"
     Optimized_Codon 8719..8721
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 8773..8775
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCC"
     Optimized_Codon 8800..8802
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 8803..8805
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 8815..8817
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 8818..8820
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 8821..8823
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     Optimized_Codon 8836..8838
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     primer_bind     8850..8870
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="2291504320095085574612-9913-4801-bed0-d
                     3b3b9f74c4a"
                     /%GC="52.4"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATTCAGCCATGCCAGAGATCC"
                     /label="PCR_0262"
     Optimized_Codon 8854..8856
                     /Relative_Adaptiveness="1.0"
                     /Original="TCG"
                     /label="AGC"
     Optimized_Codon 8884..8886
                     /Relative_Adaptiveness="1.0"
                     /Original="CAA"
                     /label="CAG"
     Optimized_Codon 8893..8895
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     Optimized_Codon 8899..8901
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 8902..8904
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 8908..8910
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 8911..8913
                     /Relative_Adaptiveness="1.0"
                     /Original="AGT"
                     /label="AGC"
     Optimized_Codon 8914..8916
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 8917..8919
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 8932..8934
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 8947..8949
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 8953..8955
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 8962..8964
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 8965..8967
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATC"
     Optimized_Codon 8977..8979
                     /Relative_Adaptiveness="1.0"
                     /Original="CGA"
                     /label="CGG"
     Optimized_Codon 8980..8982
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 8983..8985
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 8986..8988
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 9025..9027
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 9043..9045
                     /Relative_Adaptiveness="1.0"
                     /Original="CAA"
                     /label="CAG"
     Optimized_Codon 9046..9048
                     /Relative_Adaptiveness="1.0"
                     /Original="AGT"
                     /label="AGC"
     Optimized_Codon 9049..9051
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 9067..9069
                     /Relative_Adaptiveness="1.0"
                     /Original="CTA"
                     /label="CTG"
     Optimized_Codon 9091..9093
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 9112..9114
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 9121..9123
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 9133..9135
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     Optimized_Codon 9139..9141
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 9145..9147
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     Optimized_Codon 9151..9153
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 9163..9165
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 9172..9174
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 9175..9177
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 9199..9201
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 9208..9210
                     /Relative_Adaptiveness="1.0"
                     /Original="CAA"
                     /label="CAG"
     Optimized_Codon 9220..9222
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 9223..9225
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 9229..9231
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 9235..9237
                     /Relative_Adaptiveness="1.0"
                     /Original="TCA"
                     /label="AGC"
     Optimized_Codon 9238..9240
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     Optimized_Codon 9271..9273
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     Optimized_Codon 9277..9279
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 9280..9282
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 9283..9285
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 9289..9291
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 9292..9294
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 9307..9309
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     primer_bind     9310..9330
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="229150973120934bd4ac7d-8129-434d-af8f-9
                     0a057aabdeb"
                     /%GC="52.4"
                     /Tm="58.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AAGGTGGTGGATCTGGATACC"
                     /label="PCR_0263"
     Optimized_Codon 9313..9315
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 9337..9339
                     /Relative_Adaptiveness="1.0"
                     /Original="ACG"
                     /label="ACC"
     Optimized_Codon 9346..9348
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 9352..9354
                     /Relative_Adaptiveness="1.0"
                     /Original="CAA"
                     /label="CAG"
     Optimized_Codon 9376..9378
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 9394..9396
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 9400..9402
                     /Relative_Adaptiveness="1.0"
                     /Original="GTA"
                     /label="GTG"
     Optimized_Codon 9409..9411
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     Optimized_Codon 9415..9417
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 9427..9429
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 9448..9450
                     /Relative_Adaptiveness="1.0"
                     /Original="CTA"
                     /label="CTG"
     Optimized_Codon 9463..9465
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATC"
     Optimized_Codon 9466..9468
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     Optimized_Codon 9478..9480
                     /Relative_Adaptiveness="1.0"
                     /Original="GAA"
                     /label="GAG"
     Optimized_Codon 9499..9501
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 9544..9546
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     Optimized_Codon 9550..9552
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCC"
     Optimized_Codon 9556..9558
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 9568..9570
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 9571..9573
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 9574..9576
                     /Relative_Adaptiveness="1.0"
                     /Original="CAA"
                     /label="CAG"
     Optimized_Codon 9595..9597
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCC"
     Optimized_Codon 9598..9600
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 9601..9603
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 9604..9606
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCC"
     Optimized_Codon 9607..9609
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 9610..9612
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 9628..9630
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 9634..9636
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 9646..9648
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 9649..9651
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 9652..9654
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 9655..9657
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 9670..9672
                     /Relative_Adaptiveness="1.0"
                     /Original="ACG"
                     /label="ACC"
     Optimized_Codon 9676..9678
                     /Relative_Adaptiveness="1.0"
                     /Original="ACG"
                     /label="ACC"
     Optimized_Codon 9700..9702
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 9706..9708
                     /Relative_Adaptiveness="1.0"
                     /Original="AGT"
                     /label="AGC"
     Optimized_Codon 9709..9711
                     /Relative_Adaptiveness="1.0"
                     /Original="CAA"
                     /label="CAG"
     Optimized_Codon 9712..9714
                     /Relative_Adaptiveness="1.0"
                     /Original="GTA"
                     /label="GTG"
     Optimized_Codon 9721..9723
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 9730..9732
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 9742..9744
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 9760..9762
                     /Relative_Adaptiveness="1.0"
                     /Original="GTA"
                     /label="GTG"
     Optimized_Codon 9763..9765
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     Optimized_Codon 9769..9771
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 9772..9774
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 9784..9786
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 9790..9792
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCC"
     Optimized_Codon 9811..9813
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 9814..9816
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATC"
     misc_feature    9847..9912
                     /created_by="User"
                     /Sequence="GGATCCGGCGCAACAAACTTCTCTCTGCTGAAACAAGCCGGAGATGT
                     CGAAGAGAATCCTGGACCG"
                     /Color="#c72c6e"
                     /modified_by="User"
                     /label="(GSG)_P2A"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     9875..9895
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="229151377326942d7c0e91-594e-45ad-bb1a-c
                     6262e5a9a32"
                     /%GC="52.4"
                     /Tm="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.1"
                     /Sequence="TGAAACAAGCCGGAGATGTCG"
                     /label="PCR_0266"
     misc_feature    9913..1
                     /Sequence="ATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGT
                     CGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAG
                     GACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGG
                     ACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTTGGTGCGCGGCCTGGACGAGCT
                     GTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCC
                     ATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCG
                     GCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGA"
                     /created_by="User"
                     /Transferred_From="Zeocin"
                     /Transferred_Similarity="99.73%"
                     /label="Zeocin"
                     /note="Geneious type: primer_bind_reverse"
     Optimized_Codon 9922..9924
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 9928..9930
                     /Relative_Adaptiveness="1.0"
                     /Original="AGT"
                     /label="AGC"
     Optimized_Codon 9934..9936
                     /Relative_Adaptiveness="1.0"
                     /Original="GTT"
                     /label="GTG"
     Optimized_Codon 9937..9939
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     Optimized_Codon 9943..9945
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 9949..9951
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 9958..9960
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 9967..9969
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 9970..9972
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 9991..9993
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 10000..10002
                     /Relative_Adaptiveness="1.0"
                     /Original="TCC"
                     /label="AGC"
     Optimized_Codon 10030..10032
                     /Relative_Adaptiveness="1.0"
                     /Original="GGT"
                     /label="GGC"
     Optimized_Codon 10036..10038
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 10066..10068
                     /Relative_Adaptiveness="1.0"
                     /Original="GCG"
                     /label="GCC"
     Optimized_Codon 10069..10071
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 10087..10089
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     Optimized_Codon 10147..10149
                     /Relative_Adaptiveness="1.0"
                     /Original="TCG"
                     /label="AGC"
     Optimized_Codon 10153..10155
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTG"
     Optimized_Codon 10162..10164
                     /Relative_Adaptiveness="1.0"
                     /Original="ACG"
                     /label="ACC"
     Optimized_Codon 10186..10188
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     Optimized_Codon 10213..10215
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     Optimized_Codon 10243..10245
                     /Relative_Adaptiveness="1.0"
                     /Original="CCG"
                     /label="CCC"
     misc_feature    complement(10270..1)
                     /Mismatches=0
                     /Extension="TTGATTACCGATAAGCTTGATATCGAAT"
                     /created_by="primer3"
                     /annotation_group="2092367944128788a4625a-f5c2-4597-85a7-3
                     eadb98920b6"
                     /%GC="66.7"
                     /Tm="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCAGTCCTGCTCCTCGGC"
                     /label="GA_REV"
                     /note="Geneious type: primer_bind_reverse"
ORIGIN      
        1 aattcgatat caagcttatc ggtaatcaac ctctggatta caaaatttgt gaaagattga
       61 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
      121 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
      181 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
      241 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
      301 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
      361 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
      421 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
      481 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
      541 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
      601 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
      661 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
      721 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
      781 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
      841 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
      901 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
      961 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     1021 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     1081 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     1141 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     1201 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     1261 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     1321 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1381 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1441 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1501 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1561 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     1621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1681 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     1801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     1861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     1921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     1981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2041 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2101 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2161 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2221 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2281 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     2341 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     2401 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     2461 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     2521 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     2581 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     2641 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     2701 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     2761 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     2821 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     2881 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     2941 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     3001 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3061 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     3121 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3181 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3241 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3301 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3361 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3421 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3481 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3961 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4141 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4201 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4261 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4321 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4381 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4441 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4501 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4561 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4621 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4681 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4741 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4801 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4861 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4921 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4981 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5041 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5101 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5161 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5221 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5281 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     5341 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     5401 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     5461 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     5521 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     5581 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     5641 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     5701 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     5761 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     5821 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     5881 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     5941 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     6001 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     6061 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     6121 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     6181 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6241 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6301 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     6361 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     6421 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     6481 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     6541 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     6601 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     6661 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     6721 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     6781 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     6841 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     6901 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     6961 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     7021 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     7081 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     7141 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     7201 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     7261 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     7321 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     7381 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     7441 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     7501 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     7561 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     7621 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     7681 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
     7741 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     7801 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     7861 aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt agctagctag
     7921 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
     7981 acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta gagaaggtgg
     8041 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     8101 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     8161 gccagaacac aggaccggtt ctagagcgct gccaccatgg aggacgccaa aaacatcaag
     8221 aaaggccccg ccccattcta tcccctggaa gatggaaccg ccggagagca gctgcataag
     8281 gccatgaaga gatacgccct ggtgcctgga acaattgcct ttacagatgc ccatatcgag
     8341 gtggacatca cctacgccga gtacttcgaa atgtccgtgc ggctggccga agccatgaaa
     8401 cggtatgggc tgaatacaaa tcacagaatc gtggtgtgca gcgaaaactc tctgcagttc
     8461 tttatgcccg tgctgggcgc cctgtttatc ggagtggccg tggcccccgc caacgacatt
     8521 tataatgaac gggaactgct gaacagcatg ggcattagcc agcctaccgt ggtgttcgtg
     8581 tccaaaaagg ggctgcagaa aattctgaac gtgcagaaaa agctgccaat catccagaaa
     8641 attattatca tggattctaa aaccgattac cagggatttc agagcatgta caccttcgtg
     8701 acatctcatc tgcctcccgg ctttaatgaa tacgattttg tgccagagtc cttcgatagg
     8761 gacaagacaa ttgccctgat catgaactcc tctggatcta ccggcctgcc taaaggcgtg
     8821 gccctgcctc atagaaccgc ctgcgtgaga ttcagccatg ccagagatcc tatttttggc
     8881 aatcagatca ttcccgatac cgccattctg agcgtggtgc cattccatca cggctttgga
     8941 atgtttacca cactgggata tctgatctgt ggatttcggg tggtgctgat gtatagattt
     9001 gaagaagagc tgtttctgag gagcctgcag gattacaaga ttcagagcgc cctgctggtg
     9061 ccaaccctgt tctccttctt cgccaaaagc accctgattg acaaatacga tctgtctaat
     9121 ctgcacgaaa ttgcctctgg cggcgccccc ctgtctaagg aagtggggga agccgtggcc
     9181 aagaggttcc atctgccagg catcaggcag ggatatgggc tgaccgagac cacaagcgcc
     9241 attctgatta cacccgaggg ggatgataaa cccggcgccg tgggcaaagt ggtgccattt
     9301 tttgaagcca aggtggtgga tctggatacc gggaaaaccc tgggcgtgaa tcagagaggc
     9361 gaactgtgtg tgagaggccc tatgattatg tccggctatg tgaacaatcc cgaagccacc
     9421 aacgccctga ttgacaagga tggatggctg cattctggag acatcgccta ctgggacgag
     9481 gacgaacact tcttcatcgt ggaccgcctg aagtctctga ttaagtacaa aggctatcag
     9541 gtggcccccg ccgaactgga atccatcctg ctgcagcacc ccaacatctt cgacgccggc
     9601 gtggccggcc tgcccgacga tgacgccggc gaactgcccg ccgccgtggt ggtgctggag
     9661 cacggaaaga ccatgaccga aaaagagatc gtggattacg tggccagcca ggtgacaacc
     9721 gccaaaaagc tgcgcggagg agtggtgttt gtggacgaag tgcccaaagg cctgaccgga
     9781 aaactggacg ccagaaaaat cagagagatc ctgatcaagg ccaagaaggg cggaaagatc
     9841 gccgtgggat ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag
     9901 aatcctggac cgatggccaa gctgaccagc gccgtgcccg tgctgaccgc ccgcgacgtg
     9961 gccggagccg tggagttctg gaccgaccgg ctggggttca gccgggactt cgtggaggac
    10021 gacttcgccg gcgtggtgcg ggacgacgtg accctgttca tcagcgccgt gcaggaccag
    10081 gtggtgcccg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
    10141 gagtggagcg aggtggtgtc caccaacttc cgggacgcct ccgggcccgc catgaccgag
    10201 atcggcgagc agccctgggg gcgggagttc gccctgcgcg accccgccgg caactgcgtg
    10261 cacttcgtgg ccgaggagca ggactg
//
