LOCUS       PD0029_pIVTRup(BsmBI)_SpCas9        8388 bp    DNA     circular UNA 07-JAN-2020
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...50-bsz22p6
VERSION     urn.local...50-bsz22p6
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     origin          90^91
                     /label="origin"
     promoter        165..183
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     235..295
                     /Sequence="TTGGACCCTCGTACAGAAGCTAATACGACTCACTATAGGGAAATAAG
                     AGAGAAAAGAAGAG"
                     /Mismatches=0
                     /annotation_group="39127da7-3985-4fba-a322-fd27be1738dc"
                     /label="pIVTRup-BsmBI-FWD"
     promoter        255..273
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     cds             272..>301
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3"
                     /Transferred_Similarity="100.00%"
                     /label="3'UTR"
     misc_feature    302..4520
                     /Source="referenceToDocument: urn:local:.:wz-bsz22s9"
                     /label="52962_SpCas9 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    301^302
                     /Original_Bases="GAAATATAAGAGCCACCGAT"
                     /label="GAAATATAAGAGCCACCGAT"
                     /note="Geneious type: Editing History Deletion"
     CDS             322..4425
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /label="Cas9"
     primer_bind     322..346
                     /Mismatches=0
                     /Extension="GAAATATAAGAGCCACCGAT"
                     /created_by="primer3"
                     /annotation_group="243350792457124ecc1cce-69ce-460f-805b-e
                     4d233b898a5"
                     /%GC="52.0"
                     /Tm="65.1"
                     /Hairpin_Tm="46.4"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATGGACAAGAAGTACAGCATCGGCC"
                     /label="SpCas9_FWD"
     primer_bind     672..691
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="6705341217459b1f0eb0b-bb49-4f05-87a5-ad
                     6028e51052"
                     /%GC="55.0"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CATCTTCGGCAACATCGTGG"
                     /label="PCR_0268"
     primer_bind     1244..1264
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="67053900402627616d3e1-ee18-4154-b58b-20
                     06f5e8cf04"
                     /%GC="52.4"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGAACACCGAGATCACCAAGG"
                     /label="PCR_0269"
     primer_bind     1723..1743
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="670542775887745f53235-ccb5-44a7-afcd-b0
                     bf2be84cd1"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AAGAGCGAGGAAACCATCACC"
                     /label="PCR_0270"
     primer_bind     2183..2203
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="67054708610744c44978b-0cde-46ef-bccf-9c
                     afa22eda83"
                     /%GC="52.4"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGACCCTGACACTGTTTGAGG"
                     /label="PCR_0271"
     primer_bind     2675..2695
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="67055222714530714c55d-cd60-4526-bb48-71
                     4595a5bb5a"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.6"
                     /Sequence="AAGAGGGCATCAAAGAGCTGG"
                     /label="PCR_0272"
     primer_bind     3185..3205
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="67055645652374492ee1f-18de-4186-a1b6-80
                     2ad1969840"
                     /%GC="52.4"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGATCACCCTGAAGTCCAAGC"
                     /label="PCR_0273"
     primer_bind     3694..3715
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="67057308809471ee2e4a9-1074-4243-8557-92
                     f6522bcdc1"
                     /%GC="54.5"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="13.0"
                     /Sequence="GACTGGGACCCTAAGAAGTACG"
                     /label="PCR_0281"
     primer_bind     4232..4252
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="6705609527660964e7e26-e2e1-43e1-862c-ea
                     dee0b610fb"
                     /%GC="52.4"
                     /Tm="59.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGCAGGCCGAGAATATCATCC"
                     /label="PCR_0275"
     CDS             4426..4473
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /translation="KRPAATKKAGQAKKKK"
                     /label="nucleoplasmin NLS"
     misc_feature    complement(4471..4497)
                     /Mismatches=0
                     /Extension="GCCCCGCAGAAGGCAGCGATTTA"
                     /created_by="primer3"
                     /annotation_group="2433514319164137cc9d09-58e2-4676-bee9-a
                     d0a2d47e6b6"
                     /%GC="33.3"
                     /Tm="58.2"
                     /Hairpin_Tm="38.3"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTTATCGTCATCGTCTTTGTAATCTTT"
                     /label="SpCas9_REV"
                     /note="Geneious type: primer_bind_reverse"
     CDS             4474..4497
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase
                      cleavage site"
                     /translation="DYKDDDDK"
                     /label="FLAG"
     misc_feature    4521..301
                     /Source="referenceToDocument: urn:local:.:x0-bsz22s9"
                     /label="Digestion of 101362 - pIVTRup PCR BsmBI 2
                     (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    4520^4521
                     /Original_Bases="ATCGCTGCCTTCTGCGGGGC"
                     /label="ATCGCTGCCTTCTGCGGGGC"
                     /note="Geneious type: Editing History Deletion"
     cds             <4521..4607
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy; dCas9-VP64"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3, dCas9-VP64"
                     /Transferred_Similarity="100.00%"
                     /label="5'UTR"
     misc_feature    complement(4592..4662)
                     /Sequence="TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
                     TTTTTTTTCTAGACCCTCACTTCC"
                     /Mismatches=0
                     /annotation_group="e010886a-ee8c-4b16-bcf4-0ed857ebe4de"
                     /label="pIVTRup-BsmBI-REV"
                     /note="Geneious type: primer_bind_reverse"
     3'UTR           4608..4662
                     /created_by="User"
                     /label="polyA(55)"
     misc_feature    complement(4664..4669)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     protein_bind    4808..4824
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4832..4862)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4877..4898
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5186..5774)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     rep_origin      complement(7961..1)
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN      
        1 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
      181 agggcgaatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatttggac
      241 cctcgtacag aagctaatac gactcactat agggaaataa gagagaaaag aagagtaaga
      301 agaaatataa gagccaccga tatggacaag aagtacagca tcggcctgga catcggcacc
      361 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
      421 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
      481 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
      541 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
      601 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
      661 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
      721 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
      781 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
      841 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
      901 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
      961 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
     1021 aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
     1081 agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac
     1141 gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc
     1201 aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc
     1261 aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc
     1321 ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac
     1381 cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga agagttctac
     1441 aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg
     1501 aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag
     1561 atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg
     1621 aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc
     1681 cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc
     1741 accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag
     1801 cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg
     1861 ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga
     1921 atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc
     1981 aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag
     2041 tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca
     2101 taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag
     2161 gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag
     2221 gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg
     2281 cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag
     2341 cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc
     2401 atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg
     2461 tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt
     2521 aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg
     2581 cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga
     2641 cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc
     2701 cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg
     2761 tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg
     2821 tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga ctccatcgac
     2881 aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa
     2941 gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc
     3001 cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag
     3061 gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag
     3121 atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg
     3181 aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac
     3241 aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg
     3301 ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac
     3361 aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc
     3421 gccaagtact tcttctacag caacatcatg aactttttca agaccgagat taccctggcc
     3481 aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg
     3541 tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat
     3601 atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag
     3661 aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc
     3721 ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag
     3781 tccaagaaac tgaagagtgt gaaagagcta ctggggatca ccatcatgga aagaagcagc
     3841 ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac
     3901 ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg
     3961 ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg
     4021 aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag
     4081 cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc
     4141 agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc
     4201 tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt
     4261 accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg
     4321 aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc
     4381 ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacaagcg acctgccgcc
     4441 acaaagaagg ctggacaggc taagaagaag aaagattaca aagacgatga cgataagtaa
     4501 atcgctgcct tctgcggggc ttgccttctg gccatgccct tcttctctcc cttgcacctg
     4561 tacctcttgg tctttgaata aagcctgagt aggaagtgag ggtctagaaa aaaaaaaaaa
     4621 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aatgagacgc gatcaataac
     4681 cgacactcct gcgtcgccta cgcgctacga tctgcagaat tccagcacac tggcggccgt
     4741 tactagtgga tccgagctcg gtaccaagct tggcgtaatc atggtcatag ctgtttcctg
     4801 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4861 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     4921 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     4981 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5041 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5101 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5161 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5221 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5281 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5341 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5401 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5461 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5521 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5581 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5641 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5701 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5761 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5821 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5881 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5941 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6001 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6061 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6121 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6181 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6241 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6301 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6361 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6421 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6481 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6541 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6601 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6661 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6721 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6781 cgacacggaa atgttgaata ctcatactct tcctttttca attcagaaga actcgtcaag
     6841 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     6901 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     6961 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     7021 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
     7081 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     7141 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     7201 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     7261 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgggatgaca ggagatcctg
     7321 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     7381 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag
     7441 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     7501 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     7561 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg
     7621 aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg
     7681 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     7741 agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt
     7801 aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc
     7861 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt
     7921 ccgcttcctt tagcagccct tgcgccctga attttgttaa aattcgcgtt aaatttttgt
     7981 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     8041 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     8101 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     8161 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     8221 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     8281 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     8341 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcg
//
