LOCUS       pEP0003_pIVTRup(BsmBI)_ABEmax        9618 bp    DNA     circular UNA 01-JUL-2019
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...3j-br7smtl
VERSION     urn.local...3j-br7smtl
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..4155
                     /Source="referenceToDocument: urn:local:.:sc-br7sn3q"
                     /label="PCR_01_Backbone Copy for Gibson  (concatenated
                     sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    0^1
                     /Original_Bases="ATCGCTGCCTTCTGCGGGGCATCGCTGCCTTCTGCGGGGCT
                     TGCCT"
                     /label="ATCGCTGCCTTCTGCGGGGCATCGCTGCCTTCTGCGGGGCTTGCCT"
                     /note="Geneious type: Editing History Deletion"
     cds             <1..81
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy; dCas9-VP64"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3, dCas9-VP64"
                     /Transferred_Similarity="100.00%"
                     /label="5'UTR"
     misc_feature    complement(59..81)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1940643251764a2282390-e0be-41e4-816a-c9
                     39f57d18ba"
                     /%GC="52.2"
                     /Tm="58.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTAGACCCTCACTTCCTACTCAG"
                     /label="pIVTRup_BsmBI_insert_REV"
                     /note="Geneious type: primer_bind_reverse"
     3'UTR           82..136
                     /created_by="User"
                     /label="polyA(55)"
     misc_feature    complement(138..143)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     protein_bind    282..298
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(306..336)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    351..372
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(660..1248)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     rep_origin      complement(3435..3863)
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4027..4045
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     4038..4056
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="194060538554291cd25bd-008e-4b0d-8310-94
                     3cf368075b"
                     /%GC="52.6"
                     /Tm="57.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.4"
                     /Sequence="ACTATAGGGCGAATTGGGC"
                     /label="pIVTRup_BsmBI_insert_FWD"
     promoter        4117..4135
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     cds             4134..>4155
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3"
                     /Transferred_Similarity="100.00%"
                     /label="3'UTR"
     misc_feature    4156..9618
                     /Source="referenceToDocument: urn:local:.:sb-br7sn3q"
                     /label="PCR_04_ABEmax (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    4155^4156
                     /Original_Bases="AGTAAGAAGAAATATAAGAGCCACC"
                     /label="AGTAAGAAGAAATATAAGAGCCACC"
                     /note="Geneious type: Editing History Deletion"
     CDS             4217..4237
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     primer_bind     4447..4467
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1940487817495652a2faa-bafa-4f22-a7e4-34
                     32bf80214e"
                     /%GC="52.4"
                     /Tm="59.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.5"
                     /Sequence="GAATTACCGCCTGATCGATGC"
                     /label="base_editing_valid50"
     primer_bind     4937..4958
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1940525158931be434c3b-3cc3-4303-b7e1-30
                     8e631aaf3d"
                     /%GC="50.0"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="17.2"
                     /Sequence="AACAATAGAGTGATCGGCGAGG"
                     /label="base_editing_valid51"
     primer_bind     5293..5314
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1940561545653e7f34152-7eb6-457d-bcab-c7
                     1b29912225"
                     /%GC="50.0"
                     /Tm="59.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTTCAATGCTCAGAAGAAGGCC"
                     /label="base_editing_valid52"
     CDS             5426..9526
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the
                      Streptococcus pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided single strand nicks in DNA due
                      to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
                     SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHM
                     IKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKS
                     RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDN
                     LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLL
                     KALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKL
                     NREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
                     VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
                     KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKED
                     YFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTL
                     FEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFL
                     KSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTV
                     KVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEH
                     PVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVL
                     TRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAG
                     FIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFY
                     KVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSM
                     PQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVA
                     KVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFE
                     LENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHK
                     HYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAP
                     AAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /label="Cas9(D10A)"
     primer_bind     5541..5561
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="193980902951204b5f157-f48a-4d40-b813-af
                     7c25b68e31"
                     /%GC="52.4"
                     /Tm="59.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGCACAGCATCAAGAAGAACC"
                     /label="base_editing_valid24"
     primer_bind     5621..5641
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="19401370365962b99cff2-da7d-4bf9-97b8-37
                     d2cc180823"
                     /%GC="52.4"
                     /Tm="59.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACCGCCAGAAGAAGATACACC"
                     /label="base_editing_valid37"
     primer_bind     6116..6137
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="19398477043933959c8f1-c6cb-4c74-8393-b1
                     1b211e839b"
                     /%GC="50.0"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.3"
                     /Sequence="GAGAAGAAGAATGGCCTGTTCG"
                     /label="base_editing_valid25"
     primer_bind     6118..6138
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="194017432028475ede663-e26c-4a36-8516-66
                     0b0882134e"
                     /%GC="52.4"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.3"
                     /Sequence="GAAGAAGAATGGCCTGTTCGG"
                     /label="base_editing_valid38"
     primer_bind     6591..6611
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1939887410231830a1a91-50e4-4b34-95e7-39
                     04f8066e4e"
                     /%GC="52.4"
                     /Tm="59.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.0"
                     /Sequence="TCGTGAAGCTGAACAGAGAGG"
                     /label="base_editing_valid26"
     primer_bind     6594..6614
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="19402157880293a8aa8d3-1f5d-4a42-8c49-6b
                     4e1afbaa4f"
                     /%GC="52.4"
                     /Tm="59.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.0"
                     /Sequence="TGAAGCTGAACAGAGAGGACC"
                     /label="base_editing_valid39"
     primer_bind     6915..6935
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="193992579025851d80b93-e04c-411f-88b4-7a
                     fd2b9de323"
                     /%GC="52.4"
                     /Tm="60.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCGATAAGAACCTGCCCAACG"
                     /label="base_editing_valid27"
     primer_bind     7071..7091
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1940255057642a34dbf78-e7a0-42bc-a162-d9
                     735d26127a"
                     /%GC="52.4"
                     /Tm="60.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACCTGCTGTTCAAGACCAACC"
                     /label="base_editing_valid40"
     primer_bind     7485..7505
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="193996840565450d9bdb7-5f26-4d39-8744-8b
                     e8943a0db4"
                     /%GC="47.6"
                     /Tm="59.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCGCCAACAGAAACTTCATGC"
                     /label="base_editing_valid28"
     primer_bind     7497..7517
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="194029260729332407f17-56b9-4cb6-b0ba-9c
                     ba2221e19e"
                     /%GC="52.4"
                     /Tm="61.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="20.4"
                     /Sequence="ACTTCATGCAGCTGATCCACG"
                     /label="base_editing_valid41"
     primer_bind     7971..7991
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="19403305920326dc65582-bcbf-41be-9626-7b
                     117c48fd14"
                     /%GC="52.4"
                     /Tm="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACTCCATCGACAACAAGGTGC"
                     /label="base_editing_valid42"
     primer_bind     8055..8075
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="19400079824524f36d674-3ff0-4f3e-8e5b-c6
                     9c2a90f53e"
                     /%GC="52.4"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGATGAAGAACTACTGGCGGC"
                     /label="base_editing_valid29"
     primer_bind     8457..8477
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1940369245456ef9b194e-3dca-4892-a92d-f4
                     2682fd3054"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="11.6"
                     /Sequence="ACTACAAGGTGTACGACGTGC"
                     /label="base_editing_valid43"
     primer_bind     8557..8579
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="19400530868323bddefa9-87dd-49a8-9228-83
                     79ecbadfdb"
                     /%GC="47.8"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTTCAAGACCGAGATTACCCTGG"
                     /label="base_editing_valid30"
     primer_bind     9015..9035
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1940410043268cbcd1cf9-670d-4f86-ab95-61
                     78a622b1c7"
                     /%GC="52.4"
                     /Tm="59.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGCCTAAGTACTCCCTGTTCG"
                     /label="base_editing_valid44"
     primer_bind     9118..9138
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="19400980178617e0a0d2b-649e-4fd0-b15a-4a
                     aaeb49e1d8"
                     /%GC="52.4"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGTGAACTTCCTGTACCTGGC"
                     /label="base_editing_valid31"
     primer_bind     9336..9356
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="19404486511113c50ef5f-484c-4bf6-82c5-0e
                     4031bcd7dc"
                     /%GC="52.4"
                     /Tm="59.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGGCCGAGAATATCATCCACC"
                     /label="base_editing_valid45"
     CDS             9569..9589
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
ORIGIN      
        1 tctggccatg cccttcttct ctcccttgca cctgtacctc ttggtctttg aataaagcct
       61 gagtaggaag tgagggtcta gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      121 aaaaaaaaaa aaaaaatgag acgcgatcaa taaccgacac tcctgcgtcg cctacgcgct
      181 acgatctgca gaattccagc acactggcgg ccgttactag tggatccgag ctcggtacca
      241 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      301 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      361 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      421 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      481 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      541 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      661 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      781 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      841 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      901 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      961 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1021 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1081 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1141 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1201 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1261 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1321 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1381 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     1441 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     1501 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     1561 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     1621 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     1681 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     1741 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1801 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     1861 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     1921 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     1981 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2041 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2101 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2161 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2221 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     2281 ctcttccttt ttcaattcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     2341 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     2401 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     2461 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     2521 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
     2581 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     2641 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     2701 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     2761 cggcaggagc aaggtgggat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     2821 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     2881 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
     2941 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     3001 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     3061 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     3121 cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     3181 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     3241 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
     3301 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc
     3361 agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc
     3421 ctgaattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3481 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3541 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3601 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3661 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3721 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3781 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3841 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg
     3901 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     3961 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     4021 gaattgtaat acgactcact atagggcgaa ttgggccctc tagatgcatg ctcgagcggc
     4081 cgccagtgtg atggatttgg accctcgtac agaagctaat acgactcact atagggaaat
     4141 aagagagaaa agaagagtaa gaagaaatat aagagccacc atgaaacgga cagccgacgg
     4201 aagcgagttc gagtcaccaa agaagaagcg gaaagtctct gaagtcgagt ttagccacga
     4261 gtattggatg aggcacgcac tgaccctggc aaagcgagca tgggatgaaa gagaagtccc
     4321 cgtgggcgcc gtgctggtgc acaacaatag agtgatcgga gagggatgga acaggccaat
     4381 cggccgccac gaccctaccg cacacgcaga gatcatggca ctgaggcagg gaggcctggt
     4441 catgcagaat taccgcctga tcgatgccac cctgtatgtg acactggagc catgcgtgat
     4501 gtgcgcagga gcaatgatcc acagcaggat cggaagagtg gtgttcggag cacgggacgc
     4561 caagaccggc gcagcaggct ccctgatgga tgtgctgcac caccccggca tgaaccaccg
     4621 ggtggagatc acagagggaa tcctggcaga cgagtgcgcc gccctgctga gcgatttctt
     4681 tagaatgcgg agacaggaga tcaaggccca gaagaaggca cagagctcca ccgactctgg
     4741 aggatctagc ggaggatcct ctggaagcga gacaccaggc acaagcgagt ccgccacacc
     4801 agagagctcc ggcggctcct ccggaggatc ctctgaggtg gagttttccc acgagtactg
     4861 gatgagacat gccctgaccc tggccaagag ggcacgcgat gagagggagg tgcctgtggg
     4921 agccgtgctg gtgctgaaca atagagtgat cggcgagggc tggaacagag ccatcggcct
     4981 gcacgaccca acagcccatg ccgaaattat ggccctgaga cagggcggcc tggtcatgca
     5041 gaactacaga ctgattgacg ccaccctgta cgtgacattc gagccttgcg tgatgtgcgc
     5101 cggcgccatg atccactcta ggatcggccg cgtggtgttt ggcgtgagga acgcaaaaac
     5161 cggcgccgca ggctccctga tggacgtgct gcactacccc ggcatgaatc accgcgtcga
     5221 aattaccgag ggaatcctgg cagatgaatg tgccgccctg ctgtgctatt tctttcggat
     5281 gcctagacag gtgttcaatg ctcagaagaa ggcccagagc tccaccgact ccggaggatc
     5341 tagcggaggc tcctctggct ctgagacacc tggcacaagc gagagcgcaa cacctgaaag
     5401 cagcgggggc agcagcgggg ggtcagacaa gaagtacagc atcggcctgg ccatcggcac
     5461 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     5521 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     5581 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     5641 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     5701 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     5761 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     5821 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     5881 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     5941 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     6001 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     6061 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     6121 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     6181 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     6241 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     6301 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     6361 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
     6421 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     6481 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     6541 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     6601 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
     6661 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     6721 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     6781 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     6841 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     6901 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     6961 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     7021 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     7081 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     7141 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     7201 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     7261 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     7321 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     7381 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     7441 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     7501 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     7561 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     7621 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     7681 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     7741 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     7801 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     7861 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     7921 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     7981 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     8041 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     8101 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     8161 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     8221 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     8281 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     8341 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     8401 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     8461 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     8521 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     8581 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     8641 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     8701 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     8761 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     8821 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     8881 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     8941 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     9001 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     9061 gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     9121 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     9181 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     9241 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     9301 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     9361 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     9421 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     9481 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa
     9541 aagaaccgcc gacggcagcg aattcgagcc caagaagaag aggaaagtct gaatcgctgc
     9601 cttctgcggg gcttgcct
//
